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Protein

Alpha-amylase

Gene

amyA

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Displays a broad range of substrate specificity, with the capacity to hydrolyze carbohydrates as simple as maltotriose.

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

pH dependencei

Optimum pH is 6.5-7.5.

Temperature dependencei

Optimum temperature is 100 degrees Celsius. Inactive below 40 degrees Celsius. Extremely thermostable.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei124NucleophileBy similarity1
Active sitei215Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16304

Protein family/group databases

CAZyiGH57 Glycoside Hydrolase Family 57

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase (EC:3.2.1.1)
Gene namesi
Name:amyA
Ordered Locus Names:PF0272
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00001845732 – 649Alpha-amylaseAdd BLAST648

Proteomic databases

PRIDEiP49067

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi186497.PF0272

Structurei

3D structure databases

ProteinModelPortaliP49067
SMRiP49067
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 57 family.Curated

Phylogenomic databases

eggNOGiarCOG03280 Archaea
COG1449 LUCA
HOGENOMiHOG000223207
KOiK22451
OMAiHFMSAGL
OrthoDBiPOG093Z00N7

Family and domain databases

Gene3Di2.70.98.10, 1 hit
InterProiView protein in InterPro
IPR015179 A-amylase/a-glucTrfase_C
IPR015178 A-amylase/a-glucTrfase_central
IPR011013 Gal_mutarotase_sf_dom
IPR014718 GH-type_carb-bd
IPR011330 Glyco_hydro/deAcase_b/a-brl
IPR028995 Glyco_hydro_57/38_cen_sf
IPR004300 Glyco_hydro_57_N
PfamiView protein in Pfam
PF09094 DUF1925, 1 hit
PF09095 DUF1926, 1 hit
PF03065 Glyco_hydro_57, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD014405 DUF1925, 1 hit
SUPFAMiSSF74650 SSF74650, 1 hit
SSF88688 SSF88688, 1 hit
SSF88713 SSF88713, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49067-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGDKINFIFG IHNHQPLGNF GWVFEEAYEK CYWPFLETLE EYPNMKVAIH
60 70 80 90 100
TSGPLIEWLQ DNRPEYIDLL RSLVKRGQVE IVVAGFYEPV LASIPKEDRI
110 120 130 140 150
EQIRLMKEWA KSIGFDARGV WLTERVWQPE LVKTLKESGI DYVIVDDYHF
160 170 180 190 200
MSAGLSKEEL YWPYYTEDGG EVIAVFPIDE KLRYLIPFRP VDKVLEYLHS
210 220 230 240 250
LIDGDESKVA VFHDDGEKFG IWPGTYEWVY EKGWLREFFD RISSDEKINL
260 270 280 290 300
MLYTEYLEKY KPRGLVYLPI ASYFEMSEWS LPAKQARLFV EFVNELKVKG
310 320 330 340 350
IFEKYRVFVR GGIWKNFFYK YPESNYMHKR MLMVSKLVRN NPEARKYLLR
360 370 380 390 400
AQCNDAYWHG LFGGVYLPHL RRAIWNNLIK ANSYVSLGKV IRDIDYDGFE
410 420 430 440 450
EVLIENDNFY AVFKPSYGGS LVEFSSKNRL VNYVDVLARR WEHYHGYVES
460 470 480 490 500
QFDGVASIHE LEKKIPDEIR KEVAYDKYRR FMLQDHVVPL GTTLEDFMFS
510 520 530 540 550
RQQEIGEFPR VPYSYELLDG GIRLKREHLG IEVEKTVKLV NDGFEVEYIV
560 570 580 590 600
NNKTGNPVLF AVELNVAVQS IMESPGVLRG KEIVVDDKYA VGKFALKFED
610 620 630 640
EMEVWKYPVK TLSQSESGWD LIQQGVSYIV PIRLEDKIRF KLKFEEASG
Length:649
Mass (Da):76,309
Last modified:January 23, 2007 - v2
Checksum:iB75840D33D17146F
GO

Sequence cautioni

The sequence AAL80396 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22346 Unassigned DNA Translation: AAA72035.1
AE009950 Genomic DNA Translation: AAL80396.1 Different initiation.
PIRiA49512

Genome annotation databases

EnsemblBacteriaiAAL80396; AAL80396; PF0272
KEGGipfu:PF0272
PATRICifig|186497.12.peg.284

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22346 Unassigned DNA Translation: AAA72035.1
AE009950 Genomic DNA Translation: AAL80396.1 Different initiation.
PIRiA49512

3D structure databases

ProteinModelPortaliP49067
SMRiP49067
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF0272

Protein family/group databases

CAZyiGH57 Glycoside Hydrolase Family 57

Proteomic databases

PRIDEiP49067

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL80396; AAL80396; PF0272
KEGGipfu:PF0272
PATRICifig|186497.12.peg.284

Phylogenomic databases

eggNOGiarCOG03280 Archaea
COG1449 LUCA
HOGENOMiHOG000223207
KOiK22451
OMAiHFMSAGL
OrthoDBiPOG093Z00N7

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16304

Family and domain databases

Gene3Di2.70.98.10, 1 hit
InterProiView protein in InterPro
IPR015179 A-amylase/a-glucTrfase_C
IPR015178 A-amylase/a-glucTrfase_central
IPR011013 Gal_mutarotase_sf_dom
IPR014718 GH-type_carb-bd
IPR011330 Glyco_hydro/deAcase_b/a-brl
IPR028995 Glyco_hydro_57/38_cen_sf
IPR004300 Glyco_hydro_57_N
PfamiView protein in Pfam
PF09094 DUF1925, 1 hit
PF09095 DUF1926, 1 hit
PF03065 Glyco_hydro_57, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD014405 DUF1925, 1 hit
SUPFAMiSSF74650 SSF74650, 1 hit
SSF88688 SSF88688, 1 hit
SSF88713 SSF88713, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAMYA_PYRFU
AccessioniPrimary (citable) accession number: P49067
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 101 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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Main funding by: National Institutes of Health

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