UniProtKB - P49028 (MGN_DROME)
Protein
Protein mago nashi
Gene
mago
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs (PubMed:14973490, PubMed:24967911). Involved in exon definition of genes containing long introns, including the rolled/MAPK gene (PubMed:20946982, PubMed:20946983). The mago-tsu heterodimer interacts with the EJC key regulator Pym leading to EJC disassembly in the cytoplasm (PubMed:24967911). Has a role in oskar mRNA localization to the posterior pole of the developing oocyte, and may also be involved in polarization of the oocyte microtubule cytoskeleton (PubMed:8026338, PubMed:9272960).5 Publications
Miscellaneous
'Mago nashi' means 'without grandchildren' in Japanese.
GO - Molecular functioni
- RNA binding Source: UniProtKB-KW
GO - Biological processi
- maintenance of pole plasm mRNA location Source: FlyBase
- microtubule cytoskeleton organization Source: FlyBase
- mRNA splicing, via spliceosome Source: FlyBase
- mRNA transport Source: UniProtKB-KW
- negative regulation of transposition, DNA-mediated Source: FlyBase
- oocyte microtubule cytoskeleton organization Source: FlyBase
- oocyte microtubule cytoskeleton polarization Source: FlyBase
- oogenesis Source: FlyBase
- pole plasm assembly Source: FlyBase
- pole plasm oskar mRNA localization Source: FlyBase
- positive regulation of gene expression Source: FlyBase
- protein localization Source: FlyBase
- regulation of pole plasm oskar mRNA localization Source: FlyBase
- RNA splicing Source: FlyBase
Keywordsi
Molecular function | Developmental protein, RNA-binding |
Biological process | mRNA processing, mRNA splicing, mRNA transport, Transport |
Enzyme and pathway databases
Reactomei | R-DME-159236, Transport of Mature mRNA derived from an Intron-Containing Transcript R-DME-72163, mRNA Splicing - Major Pathway R-DME-72187, mRNA 3'-end processing R-DME-73856, RNA Polymerase II Transcription Termination R-DME-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
Names & Taxonomyi
Protein namesi | Recommended name: Protein mago nashi |
Gene namesi | ORF Names:CG9401 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0002736, mago |
Subcellular locationi
Nucleus
- catalytic step 2 spliceosome Source: FlyBase
- exon-exon junction complex Source: FlyBase
- nucleus Source: FlyBase
- precatalytic spliceosome Source: FlyBase
Other locations
- cytoplasm Source: FlyBase
- pole plasm Source: FlyBase
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 19 | G → R in allele mago-1; zygotic lethal. 1 Publication | 1 | |
Mutagenesisi | 21 | E → K in allele mago-93D; expression of rolled/MAPK in developing eye and wing tissue is severely reduced, and photoreceptor differentiation is impaired. 1 Publication | 1 | |
Mutagenesisi | 91 | I → T in allele mago-WE7; zygotic lethal. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000174144 | 1 – 147 | Protein mago nashiAdd BLAST | 147 |
Proteomic databases
PaxDbi | P49028 |
PRIDEi | P49028 |
Expressioni
Developmental stagei
Expressed both maternally and zygotically (PubMed:8026338). Localizes to the posterior pole of the oocyte during stage 9 of oogenesis (PubMed:14973490).2 Publications
Gene expression databases
Bgeei | FBgn0002736, Expressed in embryo and 44 other tissues |
ExpressionAtlasi | P49028, baseline and differential |
Genevisiblei | P49028, DM |
Interactioni
Subunit structurei
Heterodimer with tsu/RBM8A (PubMed:12704080, PubMed:12730685, PubMed:14968132). Part of the mRNA splicing-dependent exon junction complex (EJC) complex; the core complex contains btz/CASC3, eIF4AIII, mago and tsu/RBM8A (PubMed:14973490).
Interacts with Pym (via N-terminus); the interaction is direct (PubMed:24967911, PubMed:14968132).
Interacts with eIF4AIII (PubMed:14973490).
4 PublicationsBinary interactionsi
P49028
With | #Exp. | IntAct |
---|---|---|
tsu [Q9V535] | 6 | EBI-159609,EBI-172458 |
Protein-protein interaction databases
BioGRIDi | 63039, 19 interactors |
ComplexPortali | CPX-3100, mago-y14 exon-exon junction subcomplex CPX-3147, PYM-mago-Y14 complex CPX-3171, cdm-mago-Y14 complex |
DIPi | DIP-23439N |
IntActi | P49028, 6 interactors |
STRINGi | 7227.FBpp0071497 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P49028 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P49028 |
Family & Domainsi
Sequence similaritiesi
Belongs to the mago nashi family.Curated
Phylogenomic databases
eggNOGi | KOG3392, Eukaryota |
GeneTreei | ENSGT00390000003156 |
HOGENOMi | CLU_109497_1_1_1 |
InParanoidi | P49028 |
OMAi | EDDTNWP |
OrthoDBi | 1373136at2759 |
PhylomeDBi | P49028 |
Family and domain databases
CDDi | cd11295, Mago_nashi, 1 hit |
Gene3Di | 3.30.1560.10, 1 hit |
InterProi | View protein in InterPro IPR004023, Mago_nashi IPR036605, Mago_nashi_sf |
PANTHERi | PTHR12638, PTHR12638, 1 hit |
Pfami | View protein in Pfam PF02792, Mago_nashi, 1 hit |
SUPFAMi | SSF89817, SSF89817, 1 hit |
i Sequence
Sequence statusi: Complete.
P49028-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSTEDFYLRY YVGHKGKFGH EFLEFEFRPD GKLRYANNSN YKNDTMIRKE
60 70 80 90 100
AFVHQSVMEE LKRIIIDSEI MQEDDLPWPP PDRVGRQELE IVIGDEHISF
110 120 130 140
TTSKTGSLVD VNRSKDPEGL RCFYYLVQDL KCLVFSLIGL HFKIKPI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U03559 Genomic DNA Translation: AAC13746.1 AE013599 Genomic DNA Translation: AAF46677.1 AY071060 mRNA Translation: AAL48682.1 |
RefSeqi | NP_476636.1, NM_057288.4 |
Genome annotation databases
EnsemblMetazoai | FBtr0071569; FBpp0071497; FBgn0002736 |
GeneIDi | 37402 |
KEGGi | dme:Dmel_CG9401 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U03559 Genomic DNA Translation: AAC13746.1 AE013599 Genomic DNA Translation: AAF46677.1 AY071060 mRNA Translation: AAL48682.1 |
RefSeqi | NP_476636.1, NM_057288.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1HL6 | X-ray | 2.50 | B/D | 1-147 | [»] | |
1OO0 | X-ray | 1.85 | A | 1-147 | [»] | |
1RK8 | X-ray | 1.90 | B | 1-147 | [»] | |
2X1G | X-ray | 3.35 | B/D | 1-147 | [»] | |
SMRi | P49028 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 63039, 19 interactors |
ComplexPortali | CPX-3100, mago-y14 exon-exon junction subcomplex CPX-3147, PYM-mago-Y14 complex CPX-3171, cdm-mago-Y14 complex |
DIPi | DIP-23439N |
IntActi | P49028, 6 interactors |
STRINGi | 7227.FBpp0071497 |
Proteomic databases
PaxDbi | P49028 |
PRIDEi | P49028 |
Genome annotation databases
EnsemblMetazoai | FBtr0071569; FBpp0071497; FBgn0002736 |
GeneIDi | 37402 |
KEGGi | dme:Dmel_CG9401 |
Organism-specific databases
CTDi | 37402 |
FlyBasei | FBgn0002736, mago |
Phylogenomic databases
eggNOGi | KOG3392, Eukaryota |
GeneTreei | ENSGT00390000003156 |
HOGENOMi | CLU_109497_1_1_1 |
InParanoidi | P49028 |
OMAi | EDDTNWP |
OrthoDBi | 1373136at2759 |
PhylomeDBi | P49028 |
Enzyme and pathway databases
Reactomei | R-DME-159236, Transport of Mature mRNA derived from an Intron-Containing Transcript R-DME-72163, mRNA Splicing - Major Pathway R-DME-72187, mRNA 3'-end processing R-DME-73856, RNA Polymerase II Transcription Termination R-DME-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
Miscellaneous databases
BioGRID-ORCSi | 37402, 0 hits in 3 CRISPR screens |
EvolutionaryTracei | P49028 |
GenomeRNAii | 37402 |
PROi | PR:P49028 |
Gene expression databases
Bgeei | FBgn0002736, Expressed in embryo and 44 other tissues |
ExpressionAtlasi | P49028, baseline and differential |
Genevisiblei | P49028, DM |
Family and domain databases
CDDi | cd11295, Mago_nashi, 1 hit |
Gene3Di | 3.30.1560.10, 1 hit |
InterProi | View protein in InterPro IPR004023, Mago_nashi IPR036605, Mago_nashi_sf |
PANTHERi | PTHR12638, PTHR12638, 1 hit |
Pfami | View protein in Pfam PF02792, Mago_nashi, 1 hit |
SUPFAMi | SSF89817, SSF89817, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MGN_DROME | |
Accessioni | P49028Primary (citable) accession number: P49028 Secondary accession number(s): Q9W2L3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1996 |
Last sequence update: | February 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 172 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families