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Protein

Arachidonate 5-lipoxygenase

Gene

Alox5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.1 Publication

Catalytic activityi

Arachidonate + O2 = leukotriene A4 + H2O.

Cofactori

Protein has several cofactor binding sites:
  • Fe cationPROSITE-ProRule annotationNote: Binds 1 Fe cation per subunit.PROSITE-ProRule annotation
  • Ca2+By similarityNote: Binds 2 calcium ions per subunit.By similarity

Pathwayi: leukotriene A4 biosynthesis

This protein is involved in the pathway leukotriene A4 biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway leukotriene A4 biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi17Calcium 1; via carbonyl oxygen; structuralBy similarity1
Metal bindingi18Calcium 2; via carbonyl oxygen; structuralBy similarity1
Metal bindingi19Calcium 2; structuralBy similarity1
Metal bindingi44Calcium 2; structuralBy similarity1
Metal bindingi45Calcium 2; via carbonyl oxygen; structuralBy similarity1
Metal bindingi47Calcium 2; structuralBy similarity1
Metal bindingi79Calcium 1; via carbonyl oxygen; structuralBy similarity1
Metal bindingi80Calcium 1; via carbonyl oxygen; structuralBy similarity1
Sitei103Essential for stabilizing binding to COTL1By similarity1
Metal bindingi368Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi373Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi551Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi555Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi674Iron; via carboxylate; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • acute inflammatory response Source: MGI
  • arachidonic acid metabolic process Source: MGI
  • inflammatory response Source: MGI
  • leukotriene biosynthetic process Source: MGI
  • leukotriene metabolic process Source: MGI
  • leukotriene production involved in inflammatory response Source: MGI
  • lipoxygenase pathway Source: MGI
  • positive regulation of vasoconstriction Source: MGI
  • sensory perception of pain Source: MGI

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processLeukotriene biosynthesis
LigandCalcium, Iron, Metal-binding

Enzyme and pathway databases

BRENDAi1.13.11.34 3474
ReactomeiR-MMU-2142688 Synthesis of 5-eicosatetraenoic acids
R-MMU-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)
R-MMU-2142700 Synthesis of Lipoxins (LX)
R-MMU-6798695 Neutrophil degranulation
R-MMU-9018676 Biosynthesis of D-series resolvins
R-MMU-9018682 Biosynthesis of maresins
R-MMU-9018896 Biosynthesis of E-series 18(S)-resolvins
R-MMU-9020265 Biosynthesis of aspirin-triggered D-series resolvins
R-MMU-9023661 Biosynthesis of E-series 18(R)-resolvins
R-MMU-9026286 Biosynthesis of DPAn-3-derived protectins and resolvins
R-MMU-9026290 Biosynthesis of DPAn-3-derived maresins
R-MMU-9026403 Biosynthesis of DPAn-3-derived 13-series resolvins
R-MMU-9027604 Biosynthesis of electrophilic Omega-3 PUFA oxo-derivatives
UniPathwayiUPA00877

Names & Taxonomyi

Protein namesi
Recommended name:
Arachidonate 5-lipoxygenase (EC:1.13.11.34)
Short name:
5-LO
Short name:
5-lipoxygenase
Gene namesi
Name:Alox5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:87999 Alox5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi672V → M: Loss of activity. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL5211

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002206951 – 674Arachidonate 5-lipoxygenaseAdd BLAST674

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei272PhosphoserineBy similarity1
Modified residuei524PhosphoserineBy similarity1

Post-translational modificationi

Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-524 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP48999
PaxDbiP48999
PeptideAtlasiP48999
PRIDEiP48999

PTM databases

iPTMnetiP48999
PhosphoSitePlusiP48999

Expressioni

Gene expression databases

BgeeiENSMUSG00000025701
CleanExiMM_ALOX5
ExpressionAtlasiP48999 baseline and differential
GenevisibleiP48999 MM

Interactioni

Subunit structurei

Interacts with ALOX5AP and LTC4S. Interacts with COTL1, the interaction is required for stability and efficient catalytic activity (By similarity).By similarity

Protein-protein interaction databases

BioGridi198076, 2 interactors
STRINGi10090.ENSMUSP00000026795

Chemistry databases

BindingDBiP48999

Structurei

3D structure databases

ProteinModelPortaliP48999
SMRiP48999
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 118PLATPROSITE-ProRule annotationAdd BLAST117
Domaini119 – 674LipoxygenasePROSITE-ProRule annotationAdd BLAST556

Sequence similaritiesi

Belongs to the lipoxygenase family.Curated

Phylogenomic databases

eggNOGiENOG410IF0U Eukaryota
ENOG410YN4N LUCA
GeneTreeiENSGT00550000074415
HOGENOMiHOG000234358
HOVERGENiHBG005150
InParanoidiP48999
KOiK00461
OMAiCYRWITG
OrthoDBiEOG091G04A4
TreeFamiTF105320

Family and domain databases

InterProiView protein in InterPro
IPR000907 LipOase
IPR013819 LipOase_C
IPR036226 LipOase_C_sf
IPR020834 LipOase_CS
IPR020833 LipOase_Fe_BS
IPR001885 LipOase_mml
IPR001024 PLAT/LH2_dom
IPR036392 PLAT/LH2_dom_sf
PANTHERiPTHR11771 PTHR11771, 1 hit
PfamiView protein in Pfam
PF00305 Lipoxygenase, 1 hit
PF01477 PLAT, 1 hit
PRINTSiPR00087 LIPOXYGENASE
PR00467 MAMLPOXGNASE
SMARTiView protein in SMART
SM00308 LH2, 1 hit
SUPFAMiSSF48484 SSF48484, 1 hit
SSF49723 SSF49723, 1 hit
PROSITEiView protein in PROSITE
PS00711 LIPOXYGENASE_1, 1 hit
PS00081 LIPOXYGENASE_2, 1 hit
PS51393 LIPOXYGENASE_3, 1 hit
PS50095 PLAT, 1 hit

Sequencei

Sequence statusi: Complete.

P48999-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSYTVTVAT GSQWFAGTDD YIYLSLIGSA GCSEKHLLDK AFYNDFERGA
60 70 80 90 100
VDSYDVTVDE ELGEIYLVKI EKRKYWLHDD WYLKYITLKT PHGDYIEFPC
110 120 130 140 150
YRWITGEGEI VLRDGRAKLA RDDQIHILKQ HRRKELEARQ KQYRWMEWNP
160 170 180 190 200
GFPLSIDAKC HKDLPRDIQF DSEKGVDFVL NYSKAMENLF INRFMHMFQS
210 220 230 240 250
SWHDFADFEK IFVKISNTIS ERVKNHWQED LMFGYQFLNG CNPVLIKRCT
260 270 280 290 300
ALPPKLPVTT EMVECSLERQ LSLEQEVQEG NIFIVDYELL DGIDANKTDP
310 320 330 340 350
CTHQFLAAPI CLLYKNLANK IVPIAIQLNQ TPGESNPIFL PTDSKYDWLL
360 370 380 390 400
AKIWVRSSDF HVHQTITHLL RTHLVSEVFG IAMYRQLPAV HPLFKLLVAH
410 420 430 440 450
VRFTIAINTK AREQLICEYG LFDKANATGG GGHVQMVQRA VQDLTYSSLC
460 470 480 490 500
FPEAIKARGM DSTEDIPFYF YRDDGLLVWE AIQSFTMEVV SIYYENDQVV
510 520 530 540 550
EEDQELQDFV KDVYVYGMRG KKASGFPKSI KSREKLSEYL TVVIFTASAQ
560 570 580 590 600
HAAVNFGQYD WCSWIPNAPP TMRAPPPTAK GVVTIEQIVD TLPDRGRSCW
610 620 630 640 650
HLGAVWALSQ FQENELFLGM YPEEHFIEKP VKEAMIRFRK NLEAIVSVIA
660 670
ERNKNKKLPY YYLSPDRIPN SVAI
Length:674
Mass (Da):77,967
Last modified:July 27, 2011 - v3
Checksum:i130F27F9A77A3D88
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti466I → M in AAC37673 (PubMed:7629107).Curated1
Sequence conflicti646V → I in AAC37673 (PubMed:7629107).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42198 mRNA Translation: AAC37673.1
AK137481 mRNA Translation: BAE23373.1
AK171413 mRNA Translation: BAE42439.1
BC139102 mRNA Translation: AAI39103.1
BC141213 mRNA Translation: AAI41214.1
CCDSiCCDS20452.1
PIRiI49479
RefSeqiNP_033792.1, NM_009662.2
UniGeneiMm.41072

Genome annotation databases

EnsembliENSMUST00000026795; ENSMUSP00000026795; ENSMUSG00000025701
GeneIDi11689
KEGGimmu:11689
UCSCiuc009dkd.1 mouse

Similar proteinsi

Entry informationi

Entry nameiLOX5_MOUSE
AccessioniPrimary (citable) accession number: P48999
Secondary accession number(s): Q3TB75
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: July 27, 2011
Last modified: June 20, 2018
This is version 148 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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