UniProtKB - P48818 (ACADV_BOVIN)
Very long-chain specific acyl-CoA dehydrogenase, mitochondrial
ACADVL
Functioni
Very long-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA. Among the different mitochondrial acyl-CoA dehydrogenases, very long-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 12 to 24 carbons long primary chains.
By similarityCatalytic activityi
- a very-long-chain 2,3-saturated fatty acyl-CoA + H+ + oxidized [electron-transfer flavoprotein] = a very-long-chain (2E)-enoyl-CoA + reduced [electron-transfer flavoprotein]By similarityEC:1.3.8.9By similarityThis reaction proceeds in the forwardBy similarity direction.
- dodecanoyl-CoA + H+ + oxidized [electron-transfer flavoprotein] = (2E)-dodecenoyl-CoA + reduced [electron-transfer flavoprotein]By similarityThis reaction proceeds in the forwardBy similarity direction.
- H+ + oxidized [electron-transfer flavoprotein] + tetradecanoyl-CoA = (2E)-tetradecenoyl-CoA + reduced [electron-transfer flavoprotein]By similarityThis reaction proceeds in the forwardBy similarity direction.
- H+ + hexadecanoyl-CoA + oxidized [electron-transfer flavoprotein] = (2E)-hexadecenoyl-CoA + reduced [electron-transfer flavoprotein]By similarityThis reaction proceeds in the forwardBy similarity direction.
- H+ + octadecanoyl-CoA + oxidized [electron-transfer flavoprotein] = (2E)-octadecenoyl-CoA + reduced [electron-transfer flavoprotein]By similarityThis reaction proceeds in the forwardBy similarity direction.
- eicosanoyl-CoA + H+ + oxidized [electron-transfer flavoprotein] = (2E)-eicosenoyl-CoA + reduced [electron-transfer flavoprotein]By similarityThis reaction proceeds in the forwardBy similarity direction.
- docosanoyl-CoA + H+ + oxidized [electron-transfer flavoprotein] = (2E)-docosenoyl-CoA + reduced [electron-transfer flavoprotein]By similarityThis reaction proceeds in the forwardBy similarity direction.
- H+ + oxidized [electron-transfer flavoprotein] + tetracosanoyl-CoA = (2E)-tetracosenoyl-CoA + reduced [electron-transfer flavoprotein]By similarityThis reaction proceeds in the forwardBy similarity direction.
Cofactori
: mitochondrial fatty acid beta-oxidation Pathwayi
This protein is involved in the pathway mitochondrial fatty acid beta-oxidation, which is part of Lipid metabolism.By similarityView all proteins of this organism that are known to be involved in the pathway mitochondrial fatty acid beta-oxidation and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 462 | Proton acceptorBy similarity | 1 | |
Binding sitei | 562 | FADBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 214 – 223 | FADBy similarity | 10 | |
Nucleotide bindingi | 249 – 251 | FADBy similarity | 3 | |
Nucleotide bindingi | 464 – 466 | FADBy similarity | 3 |
GO - Molecular functioni
- acyl-CoA dehydrogenase activity Source: UniProtKB
- fatty-acyl-CoA binding Source: GO_Central
- flavin adenine dinucleotide binding Source: UniProtKB
- identical protein binding Source: UniProtKB
- very-long-chain-acyl-CoA dehydrogenase activity Source: UniProtKB
GO - Biological processi
- epithelial cell differentiation Source: Ensembl
- fatty acid beta-oxidation using acyl-CoA dehydrogenase Source: UniProtKB
- negative regulation of fatty acid biosynthetic process Source: Ensembl
- negative regulation of fatty acid oxidation Source: Ensembl
- regulation of cholesterol metabolic process Source: Ensembl
- response to cold Source: Ensembl
- temperature homeostasis Source: Ensembl
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Fatty acid metabolism, Lipid metabolism |
Ligand | FAD, Flavoprotein |
Enzyme and pathway databases
UniPathwayi | UPA00660 |
Names & Taxonomyi
Protein namesi | Recommended name: Very long-chain specific acyl-CoA dehydrogenase, mitochondrialCurated (EC:1.3.8.9By similarity)Short name: VLCADCurated |
Gene namesi | |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | HostDB:ENSBTAG00000003072 |
VGNCi | VGNC:50246, ACADVL |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane By similarity; Peripheral membrane protein By similarity
Mitochondrion
- extrinsic component of mitochondrial inner membrane Source: UniProtKB
- mitochondrial nucleoid Source: Ensembl
- mitochondrion Source: GO_Central
Nucleus
- nucleolus Source: Ensembl
- nucleoplasm Source: Ensembl
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 40 | MitochondrionBy similarityAdd BLAST | 40 | |
ChainiPRO_0000000514 | 41 – 655 | Very long-chain specific acyl-CoA dehydrogenase, mitochondrialAdd BLAST | 615 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 71 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 71 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 195 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 237 | S-nitrosocysteineBy similarity | 1 | |
Modified residuei | 239 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 239 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 276 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 276 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 278 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 278 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 298 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 331 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 331 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 372 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 482 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 482 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 517 | PhosphoserineBy similarity | 1 | |
Modified residuei | 522 | PhosphoserineBy similarity | 1 | |
Modified residuei | 550 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 556 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 556 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 639 | N6-succinyllysineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Phosphoprotein, S-nitrosylationProteomic databases
PaxDbi | P48818 |
PeptideAtlasi | P48818 |
PRIDEi | P48818 |
Expressioni
Gene expression databases
Bgeei | ENSBTAG00000003072, Expressed in corpus luteum and 96 other tissues |
ExpressionAtlasi | P48818, baseline and differential |
Interactioni
Subunit structurei
Homodimer. Homodimerizes after import into the mitochondrion.
By similarityGO - Molecular functioni
- identical protein binding Source: UniProtKB
Protein-protein interaction databases
IntActi | P48818, 35 interactors |
STRINGi | 9913.ENSBTAP00000003999 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 22 – 66 | DisorderedSequence analysisAdd BLAST | 45 | |
Regioni | 41 – 482 | CatalyticBy similarityAdd BLAST | 442 | |
Regioni | 461 – 463 | Substrate bindingBy similarity | 3 | |
Regioni | 483 – 516 | Membrane-anchoringBy similarityAdd BLAST | 34 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 47 – 66 | Polar residuesSequence analysisAdd BLAST | 20 |
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG0137, Eukaryota |
GeneTreei | ENSGT00940000158535 |
HOGENOMi | CLU_018204_11_2_1 |
InParanoidi | P48818 |
OMAi | NAFMGLR |
OrthoDBi | 819314at2759 |
TreeFami | TF105053 |
Family and domain databases
Gene3Di | 1.10.540.10, 1 hit |
InterProi | View protein in InterPro IPR006089, Acyl-CoA_DH_CS IPR006091, Acyl-CoA_Oxase/DH_cen-dom IPR036250, AcylCo_DH-like_C IPR009075, AcylCo_DH/oxidase_C IPR013786, AcylCoA_DH/ox_N IPR037069, AcylCoA_DH/ox_N_sf IPR009100, AcylCoA_DH/oxidase_NM_dom |
Pfami | View protein in Pfam PF00441, Acyl-CoA_dh_1, 1 hit PF02770, Acyl-CoA_dh_M, 1 hit PF02771, Acyl-CoA_dh_N, 1 hit |
SUPFAMi | SSF47203, SSF47203, 2 hits SSF56645, SSF56645, 1 hit |
PROSITEi | View protein in PROSITE PS00072, ACYL_COA_DH_1, 1 hit PS00073, ACYL_COA_DH_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MRAARMAQST GRQLLRLRGV SSWPGELLGQ PRPGPARRPY ASGVAQAAVD
60 70 80 90 100
QSDSQPSEAS TREKRANSVS KSFAVGTFKG QLTTDQVFPY PSVLNEDQTQ
110 120 130 140 150
FLKELVGPVT RFFEEVNDAA KNDMLERVEE TTMQGLKELG AFGLQVPNEL
160 170 180 190 200
GGVGLCNTQY ARLVEIVGMY DLGVGIVLGA HQSIGFKGIL LFGTKAQKEK
210 220 230 240 250
YLPKLASGET IAAFCLTEPS SGSDAASIRS SAVPSPCGKY YTLNGSKIWI
260 270 280 290 300
SNGGLADIFT VFAKTPVTDT ATGAVKEKIT AFVVERSFGG VTHGPPEKKM
310 320 330 340 350
GIKASNTAEV YFDGVRVPAE NVLGEVGGGF KVAMHILNNG RFGMAAALAG
360 370 380 390 400
TMKGIIAKAV DHAANRTQFG EKIHNFGLIQ EKLARMAMLQ YVTESMAYMV
410 420 430 440 450
SANMDQGSTD FQIEAAISKI FGSEAAWKVT DECIQIMGGM GFMKEPGVER
460 470 480 490 500
VLRDLRIFRI FEGTNDILRL FVALQGCMDK GKELSGLGNA LKNPFGNAGL
510 520 530 540 550
LLGEAGKQLR RRAGLGSGLS LSGIVHQELS RSGELAVQAL EQFATVVEAK
560 570 580 590 600
LIKHKKDIIN EQFLLQRLAD SAIDLYAMVV VLSRASRSLS EGHPTAQHEK
610 620 630 640 650
MLCDSWCIEA AARIRENMTA LQSDPQQQEL FRNFKSISKA LVERGGVVTS
NPLGF
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A3Q1LQ76 | A0A3Q1LQ76_BOVIN | Very long-chain-specific acyl-CoA d... | ACADVL | 634 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 2 | R → Q in AAA74051 (Ref. 1) Curated | 1 | |
Sequence conflicti | 7 – 8 | AQ → TA in AAA74051 (Ref. 1) Curated | 2 | |
Sequence conflicti | 10 | T → L in AAA74051 (Ref. 1) Curated | 1 | |
Sequence conflicti | 13 | Q → T in AAA74051 (Ref. 1) Curated | 1 | |
Sequence conflicti | 37 | R → P in AAA74051 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U30817 mRNA Translation: AAA74051.1 BT030546 mRNA Translation: ABQ12986.1 BC103104 mRNA Translation: AAI03105.1 |
RefSeqi | NP_776919.1, NM_174494.2 |
Genome annotation databases
Ensembli | ENSBTAT00000003999; ENSBTAP00000003999; ENSBTAG00000003072 |
GeneIDi | 282130 |
KEGGi | bta:282130 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U30817 mRNA Translation: AAA74051.1 BT030546 mRNA Translation: ABQ12986.1 BC103104 mRNA Translation: AAI03105.1 |
RefSeqi | NP_776919.1, NM_174494.2 |
3D structure databases
AlphaFoldDBi | P48818 |
SMRi | P48818 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P48818, 35 interactors |
STRINGi | 9913.ENSBTAP00000003999 |
Proteomic databases
PaxDbi | P48818 |
PeptideAtlasi | P48818 |
PRIDEi | P48818 |
Genome annotation databases
Ensembli | ENSBTAT00000003999; ENSBTAP00000003999; ENSBTAG00000003072 |
GeneIDi | 282130 |
KEGGi | bta:282130 |
Organism-specific databases
CTDi | 37 |
VEuPathDBi | HostDB:ENSBTAG00000003072 |
VGNCi | VGNC:50246, ACADVL |
Phylogenomic databases
eggNOGi | KOG0137, Eukaryota |
GeneTreei | ENSGT00940000158535 |
HOGENOMi | CLU_018204_11_2_1 |
InParanoidi | P48818 |
OMAi | NAFMGLR |
OrthoDBi | 819314at2759 |
TreeFami | TF105053 |
Enzyme and pathway databases
UniPathwayi | UPA00660 |
Gene expression databases
Bgeei | ENSBTAG00000003072, Expressed in corpus luteum and 96 other tissues |
ExpressionAtlasi | P48818, baseline and differential |
Family and domain databases
Gene3Di | 1.10.540.10, 1 hit |
InterProi | View protein in InterPro IPR006089, Acyl-CoA_DH_CS IPR006091, Acyl-CoA_Oxase/DH_cen-dom IPR036250, AcylCo_DH-like_C IPR009075, AcylCo_DH/oxidase_C IPR013786, AcylCoA_DH/ox_N IPR037069, AcylCoA_DH/ox_N_sf IPR009100, AcylCoA_DH/oxidase_NM_dom |
Pfami | View protein in Pfam PF00441, Acyl-CoA_dh_1, 1 hit PF02770, Acyl-CoA_dh_M, 1 hit PF02771, Acyl-CoA_dh_N, 1 hit |
SUPFAMi | SSF47203, SSF47203, 2 hits SSF56645, SSF56645, 1 hit |
PROSITEi | View protein in PROSITE PS00072, ACYL_COA_DH_1, 1 hit PS00073, ACYL_COA_DH_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ACADV_BOVIN | |
Accessioni | P48818Primary (citable) accession number: P48818 Secondary accession number(s): A5D9C7, Q3SZ66 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1996 |
Last sequence update: | November 13, 2007 | |
Last modified: | May 25, 2022 | |
This is version 154 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families