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Protein

Troponin I, cardiac muscle

Gene

Tnni3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei81Involved in TNI-TNT interactions1
Sitei98Involved in TNI-TNT interactions1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi138 – 149By similarityAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding, Muscle protein
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-390522 Striated Muscle Contraction
R-MMU-5578775 Ion homeostasis

Names & Taxonomyi

Protein namesi
Recommended name:
Troponin I, cardiac muscle
Alternative name(s):
Cardiac troponin I
Gene namesi
Name:Tnni3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:98783 Tnni3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001861532 – 211Troponin I, cardiac muscleAdd BLAST210

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei5PhosphoserineBy similarity1
Modified residuei6PhosphoserineBy similarity1
Modified residuei23Phosphoserine; by PKA and PKD/PRKD11 Publication1
Modified residuei24Phosphoserine; by PKA and PKD/PRKD11 Publication1
Modified residuei27PhosphotyrosineBy similarity1
Modified residuei32Phosphothreonine; by STK4/MST1By similarity1
Modified residuei43Phosphoserine; by PKC/PRKCE1 Publication1
Modified residuei45Phosphoserine; by PKC/PRKCE1 Publication1
Modified residuei52Phosphothreonine; by STK4/MST1By similarity1
Modified residuei79PhosphothreonineBy similarity1
Modified residuei130Phosphothreonine; by STK4/MST1By similarity1
Modified residuei144Phosphothreonine; by STK4/MST1By similarity1
Modified residuei151PhosphoserineBy similarity1
Modified residuei167PhosphoserineBy similarity1
Modified residuei200PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated at Ser-23 and Ser-24 by PRKD1; phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-32. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T) (By similarity). Phosphorylated at Ser-43 and Ser-45 by PRKCE; phosphorylation increases myocardium contractile dysfunction.By similarity2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP48787
PRIDEiP48787

PTM databases

iPTMnetiP48787
PhosphoSitePlusiP48787

Expressioni

Gene expression databases

BgeeiENSMUSG00000035458 Expressed in 116 organ(s), highest expression level in heart
CleanExiMM_TNNI3
ExpressionAtlasiP48787 baseline and differential
GenevisibleiP48787 MM

Interactioni

Subunit structurei

Interacts with TRIM63 (By similarity). Binds to actin and tropomyosin. Interacts with STK4/MST1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204266, 3 interactors
ComplexPortaliCPX-16 Cardiac Troponin complex
IntActiP48787, 6 interactors
MINTiP48787
STRINGi10090.ENSMUSP00000096458

Structurei

Secondary structure

1211
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP48787
SMRiP48787
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP48787

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni33 – 80Involved in binding TNCAdd BLAST48
Regioni130 – 151Involved in binding TNC and actinAdd BLAST22

Sequence similaritiesi

Belongs to the troponin I family.Curated

Phylogenomic databases

eggNOGiKOG3977 Eukaryota
ENOG410Y9IX LUCA
GeneTreeiENSGT00390000002746
HOGENOMiHOG000293300
HOVERGENiHBG052737
InParanoidiP48787
KOiK12044
OMAiHTRVDKV
OrthoDBiEOG091G0NOD
PhylomeDBiP48787
TreeFamiTF313374

Family and domain databases

Gene3Di1.20.5.350, 1 hit
InterProiView protein in InterPro
IPR001978 Troponin
IPR021666 Troponin-I_N
IPR038077 Troponin_sf
PfamiView protein in Pfam
PF00992 Troponin, 1 hit
PF11636 Troponin-I_N, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

P48787-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MADESSDAAG EPQPAPAPVR RRSSANYRAY ATEPHAKKKS KISASRKLQL
60 70 80 90 100
KTLMLQIAKQ EMEREAEERR GEKGRVLRTR CQPLELDGLG FEELQDLCRQ
110 120 130 140 150
LHARVDKVDE ERYDVEAKVT KNITEIADLT QKIYDLRGKF KRPTLRRVRI
160 170 180 190 200
SADAMMQALL GTRAKESLDL RAHLKQVKKE DIEKENREVG DWRKNIDALS
210
GMEGRKKKFE G
Length:211
Mass (Da):24,259
Last modified:January 23, 2007 - v2
Checksum:iFB886B0E87B8D49D
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3YXP5D3YXP5_MOUSE
Troponin I, cardiac muscle
Tnni3
125Annotation score:
D3Z160D3Z160_MOUSE
Troponin I, cardiac muscle
Tnni3
112Annotation score:
A0A140LIV0A0A140LIV0_MOUSE
Troponin I, cardiac muscle
Tnni3
152Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22784 Genomic DNA Translation: CAA80459.1
U09181 mRNA Translation: AAA19657.1
BC061171 mRNA Translation: AAH61171.1
CCDSiCCDS39736.1
PIRiA53805
RefSeqiNP_033432.1, NM_009406.4
UniGeneiMm.27674

Genome annotation databases

EnsembliENSMUST00000098859; ENSMUSP00000096458; ENSMUSG00000035458
GeneIDi21954
KEGGimmu:21954
UCSCiuc009exv.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22784 Genomic DNA Translation: CAA80459.1
U09181 mRNA Translation: AAA19657.1
BC061171 mRNA Translation: AAH61171.1
CCDSiCCDS39736.1
PIRiA53805
RefSeqiNP_033432.1, NM_009406.4
UniGeneiMm.27674

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JPWNMR-A1-32[»]
ProteinModelPortaliP48787
SMRiP48787
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204266, 3 interactors
ComplexPortaliCPX-16 Cardiac Troponin complex
IntActiP48787, 6 interactors
MINTiP48787
STRINGi10090.ENSMUSP00000096458

PTM databases

iPTMnetiP48787
PhosphoSitePlusiP48787

Proteomic databases

PaxDbiP48787
PRIDEiP48787

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000098859; ENSMUSP00000096458; ENSMUSG00000035458
GeneIDi21954
KEGGimmu:21954
UCSCiuc009exv.1 mouse

Organism-specific databases

CTDi7137
MGIiMGI:98783 Tnni3

Phylogenomic databases

eggNOGiKOG3977 Eukaryota
ENOG410Y9IX LUCA
GeneTreeiENSGT00390000002746
HOGENOMiHOG000293300
HOVERGENiHBG052737
InParanoidiP48787
KOiK12044
OMAiHTRVDKV
OrthoDBiEOG091G0NOD
PhylomeDBiP48787
TreeFamiTF313374

Enzyme and pathway databases

ReactomeiR-MMU-390522 Striated Muscle Contraction
R-MMU-5578775 Ion homeostasis

Miscellaneous databases

EvolutionaryTraceiP48787
PROiPR:P48787
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000035458 Expressed in 116 organ(s), highest expression level in heart
CleanExiMM_TNNI3
ExpressionAtlasiP48787 baseline and differential
GenevisibleiP48787 MM

Family and domain databases

Gene3Di1.20.5.350, 1 hit
InterProiView protein in InterPro
IPR001978 Troponin
IPR021666 Troponin-I_N
IPR038077 Troponin_sf
PfamiView protein in Pfam
PF00992 Troponin, 1 hit
PF11636 Troponin-I_N, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTNNI3_MOUSE
AccessioniPrimary (citable) accession number: P48787
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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