Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Sodium/calcium exchanger 2

Gene

Slc8a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates the electrogenic exchange of Ca2+ against Na+ ions across the cell membrane, and thereby contributes to the regulation of cytoplasmic Ca2+ levels and Ca2+-dependent cellular processes (PubMed:8021246, PubMed:9486131). Contributes to cellular Ca2+ homeostasis in excitable cells. Contributes to the rapid decrease of cytoplasmic Ca2+ levels back to baseline after neuronal activation, and thereby contributes to modulate synaptic plasticity, learning and memory. Plays a role in regulating urinary Ca2+ and Na+ excretion.By similarity2 Publications

Activity regulationi

Calcium transport is down-regulated by Na+ and stimulated by Ca2+.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi407Calcium 1By similarity1
Metal bindingi407Calcium 2By similarity1
Metal bindingi407Calcium 3By similarity1
Metal bindingi443Calcium 1By similarity1
Metal bindingi443Calcium 4By similarity1
Metal bindingi468Calcium 2By similarity1
Metal bindingi469Calcium 1By similarity1
Metal bindingi469Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi469Calcium 3By similarity1
Metal bindingi469Calcium 4By similarity1
Metal bindingi471Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi473Calcium 1By similarity1
Metal bindingi473Calcium 3By similarity1
Metal bindingi473Calcium 4By similarity1
Metal bindingi476Calcium 4By similarity1
Metal bindingi518Calcium 3By similarity1
Metal bindingi519Calcium 2By similarity1
Metal bindingi520Calcium 2By similarity1
Metal bindingi520Calcium 3By similarity1
Metal bindingi536Calcium 5By similarity1
Metal bindingi598Calcium 5By similarity1
Metal bindingi598Calcium 6By similarity1
Metal bindingi599Calcium 6By similarity1
Metal bindingi600Calcium 5; via carbonyl oxygenBy similarity1
Metal bindingi600Calcium 6By similarity1
Metal bindingi665Calcium 5By similarity1

GO - Molecular functioni

  • calcium:sodium antiporter activity Source: RGD
  • calmodulin binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionCalmodulin-binding
Biological processAntiport, Calcium transport, Ion transport, Sodium transport, Transport
LigandCalcium, Metal-binding, Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/calcium exchanger 2
Alternative name(s):
Na(+)/Ca(2+)-exchange protein 2
Solute carrier family 8 member 2
Gene namesi
Name:Slc8a2
Synonyms:Ncx2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620194 Slc8a2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 68ExtracellularSequence analysisAdd BLAST48
Transmembranei69 – 90HelicalSequence analysisAdd BLAST22
Topological domaini91 – 130CytoplasmicSequence analysisAdd BLAST40
Transmembranei131 – 152HelicalSequence analysisAdd BLAST22
Topological domaini153 – 164ExtracellularSequence analysisAdd BLAST12
Transmembranei165 – 185HelicalSequence analysisAdd BLAST21
Topological domaini186 – 196CytoplasmicSequence analysisAdd BLAST11
Transmembranei197 – 219HelicalSequence analysisAdd BLAST23
Topological domaini220 – 222ExtracellularSequence analysis3
Transmembranei223 – 246HelicalSequence analysisAdd BLAST24
Topological domaini247 – 720CytoplasmicSequence analysisAdd BLAST474
Transmembranei721 – 740HelicalSequence analysisAdd BLAST20
Topological domaini741 – 747ExtracellularSequence analysis7
Transmembranei748 – 770HelicalSequence analysisAdd BLAST23
Topological domaini771 – 772CytoplasmicSequence analysis2
Transmembranei773 – 791HelicalSequence analysisAdd BLAST19
Topological domaini792 – 822ExtracellularSequence analysisAdd BLAST31
Transmembranei823 – 843HelicalSequence analysisAdd BLAST21
Topological domaini844 – 854CytoplasmicSequence analysisAdd BLAST11
Transmembranei855 – 875HelicalSequence analysisAdd BLAST21
Topological domaini876 – 892ExtracellularSequence analysisAdd BLAST17
Transmembranei893 – 909HelicalSequence analysisAdd BLAST17
Topological domaini910 – 921CytoplasmicSequence analysisAdd BLAST12

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000001938321 – 921Sodium/calcium exchanger 2Add BLAST901

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi34N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei622PhosphoserineBy similarity1
Glycosylationi817N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP48768
PRIDEiP48768

PTM databases

iPTMnetiP48768
SwissPalmiP48768

Expressioni

Tissue specificityi

Detected in neocortex and hippocampus on pyramidal cells, astrocyte processes and dendrites (at protein level) (PubMed:16914199). Brain and skeletal muscle.2 Publications

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi250785, 1 interactor
IntActiP48768, 1 interactor
MINTiP48768
STRINGi10116.ENSRNOP00000042012

Structurei

3D structure databases

ProteinModelPortaliP48768
SMRiP48768
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati135 – 175Alpha-1Add BLAST41
Domaini384 – 483Calx-beta 1Add BLAST100
Domaini512 – 612Calx-beta 2Add BLAST101
Repeati790 – 826Alpha-2Add BLAST37

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni248 – 267Putative calmodulin-binding regionBy similarityAdd BLAST20

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi29 – 32Poly-Pro4
Compositional biasi638 – 641Poly-Glu4

Domaini

The cytoplasmic Calx-beta domains bind the regulatory Ca2+. The first Calx-beta domain can bind up to four Ca2+ ions. The second domain can bind another two Ca2+ ions that are essential for calcium-regulated ion exchange.By similarity

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1306 Eukaryota
ENOG410XPJP LUCA
HOGENOMiHOG000266971
HOVERGENiHBG006441
InParanoidiP48768
KOiK05849

Family and domain databases

Gene3Di2.60.40.2030, 2 hits
InterProiView protein in InterPro
IPR038081 CalX-like_sf
IPR003644 Calx_beta
IPR004836 Na_Ca_Ex
IPR032452 Na_Ca_Ex_C-exten
IPR004837 NaCa_Exmemb
PfamiView protein in Pfam
PF03160 Calx-beta, 2 hits
PF01699 Na_Ca_ex, 2 hits
PF16494 Na_Ca_ex_C, 1 hit
PRINTSiPR01259 NACAEXCHNGR
SMARTiView protein in SMART
SM00237 Calx_beta, 2 hits
SUPFAMiSSF141072 SSF141072, 2 hits
TIGRFAMsiTIGR00845 caca, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

P48768-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAPLALVGVA LLLGAPHCLG EATPTPSLPP PPANDSDASP GGCQGSYRCQ
60 70 80 90 100
PGVLLPVWEP DDPSLGDKAA RAVVYFVAMV YMFLGLSIIA DRFMASIEVI
110 120 130 140 150
TSKEKEITIT KANGETSVGT VRIWNETVSN LTLMALGSSA PEILLSVIEV
160 170 180 190 200
CGHNFQAGEL GPGTIVGSAA FNMFVVIAVC VYVIPAGESR KIKHLRVFFV
210 220 230 240 250
TASWSIFAYV WLYLILAVFS PGVVQVWEAL LTLVFFPVCV VFAWMADKRL
260 270 280 290 300
LFYKYVYKRY RTDPRSGIII GAEGDPPKSI ELDGTFVGTE VPGELGALGT
310 320 330 340 350
GPAEARELDA SRREVIQILK DLKQKHPDKD LEQLVGIAKY YALLHQQKSR
360 370 380 390 400
AFYRIQATRL MTGAGNVLRR HAADAARRPG ANDGAPDDED DGASRIFFEP
410 420 430 440 450
SLYHCLENCG SVLLSVACQG GEGNSTFYVD YRTEDGSAKA GSDYEYSEGT
460 470 480 490 500
LVFKPGETQK ELRIGIIDDD IFEEDEHFFV RLLNLRVGDA QGMFEPDGGG
510 520 530 540 550
RPKGRLVAPL LATVTILDDD HAGIFSFQDR LLHVSECMGT VDVRVVRSSG
560 570 580 590 600
ARGTVRLPYR TVDGTARGGG VHYEDACGEL EFGDDETMKT LQVKIVDDEE
610 620 630 640 650
YEKKDNFFIE LGQPQWLKRG ISALLLNQGD GDRKLTAEEE EAQRIAEMGK
660 670 680 690 700
PVLGENCRLE VIIEESYDFK NTVDKLIKKT NLALVIGTHS WREQFLEAVT
710 720 730 740 750
VSAGDEEEDE DGSREERLPS CFDYVMHFLT VFWKVLFACL PPTEYCHGWA
760 770 780 790 800
CFGVCILVIG LLTALIGDLA SHFGCTVGLK DSVNAVVFVA LGTSIPDTFA
810 820 830 840 850
SKVAALQDQC ADASIGNVTG SNAVNVFLGL GVAWSVAAVY WAVQGRPFEV
860 870 880 890 900
RTGTLAFSVT LFTVFAFVGI AVLLYRRRPH IGGELGGPRG PKLATTALFL
910 920
GLWFLYILFA SLEAYCHIRG F
Length:921
Mass (Da):100,523
Last modified:February 1, 1996 - v1
Checksum:i0CDB26BEACBCF6B1
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F1M9A2F1M9A2_RAT
Sodium/calcium exchanger 2
Slc8a2
919Annotation score:
A0A0G2JZK7A0A0G2JZK7_RAT
Sodium/calcium exchanger 2
Slc8a2
876Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08141 mRNA Translation: AAA19920.1
PIRiA54139
RefSeqiNP_511174.1, NM_078619.1
UniGeneiRn.10305

Genome annotation databases

GeneIDi140447
KEGGirno:140447
UCSCiRGD:620194 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08141 mRNA Translation: AAA19920.1
PIRiA54139
RefSeqiNP_511174.1, NM_078619.1
UniGeneiRn.10305

3D structure databases

ProteinModelPortaliP48768
SMRiP48768
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250785, 1 interactor
IntActiP48768, 1 interactor
MINTiP48768
STRINGi10116.ENSRNOP00000042012

PTM databases

iPTMnetiP48768
SwissPalmiP48768

Proteomic databases

PaxDbiP48768
PRIDEiP48768

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi140447
KEGGirno:140447
UCSCiRGD:620194 rat

Organism-specific databases

CTDi6543
RGDi620194 Slc8a2

Phylogenomic databases

eggNOGiKOG1306 Eukaryota
ENOG410XPJP LUCA
HOGENOMiHOG000266971
HOVERGENiHBG006441
InParanoidiP48768
KOiK05849

Miscellaneous databases

PROiPR:P48768

Family and domain databases

Gene3Di2.60.40.2030, 2 hits
InterProiView protein in InterPro
IPR038081 CalX-like_sf
IPR003644 Calx_beta
IPR004836 Na_Ca_Ex
IPR032452 Na_Ca_Ex_C-exten
IPR004837 NaCa_Exmemb
PfamiView protein in Pfam
PF03160 Calx-beta, 2 hits
PF01699 Na_Ca_ex, 2 hits
PF16494 Na_Ca_ex_C, 1 hit
PRINTSiPR01259 NACAEXCHNGR
SMARTiView protein in SMART
SM00237 Calx_beta, 2 hits
SUPFAMiSSF141072 SSF141072, 2 hits
TIGRFAMsiTIGR00845 caca, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNAC2_RAT
AccessioniPrimary (citable) accession number: P48768
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 10, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again