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UniProtKB - P48725 (PCNT_MOUSE)

Protein

Pericentrin

Gene

Pcnt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome.By similarity1 Publication

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionCalmodulin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Pericentrin
Gene namesi
Name:Pcnt
Synonyms:Pcnt2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:102722 Pcnt

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000582581 – 2898PericentrinAdd BLAST2898

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei44PhosphoserineBy similarity1
Modified residuei1022PhosphoserineBy similarity1
Modified residuei1437PhosphoserineCombined sources1
Modified residuei1828PhosphoserineBy similarity1
Modified residuei1859PhosphoserineCombined sources1
Modified residuei1860PhosphoserineCombined sources1
Modified residuei1959PhosphoserineBy similarity1
Modified residuei1987PhosphoserineBy similarity1
Modified residuei2128PhosphoserineBy similarity1
Modified residuei2865PhosphoserineBy similarity1

Post-translational modificationi

Cleaved during mitotis which leads to removal of CDK5RAP2 from the centrosome and promotes centriole disengagement and subsequent centriole separation. The C-terminal fragment is rapidly degraded following cleavage.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP48725
MaxQBiP48725
PaxDbiP48725
PeptideAtlasiP48725
PRIDEiP48725

PTM databases

iPTMnetiP48725
PhosphoSitePlusiP48725

Expressioni

Tissue specificityi

Expressed in heart and lung (at protein level). Expressed in kidney, thymus, liver, brain, muscle, testis, spleen, lung and heart.1 Publication

Developmental stagei

Expressed in embryo at 17 dpc (at protein level). Expressed in embryos at 7, 11, 15 and 17 dpc. Strongly expressed in brain tissues at early stages of development.2 Publications

Gene expression databases

CleanExiMM_PCNT

Interactioni

Subunit structurei

Interacts with DISC1 and PCM1. Binds calmodulin. Interacts with CEP131 (By similarity). Interacts with CDK5RAP2; the interaction is leading to centrosomal localization of PCNT and CDK5RAP2 (PubMed:20471352). Interacts with CHD3 (PubMed:17626165). Interacts with CHD4; the interaction regulates centrosome integrity (PubMed:17626165). Interacts with NEK2 (By similarity). Interacts with CCDC13 (By similarity). Interacts with CEP68 (By similarity). Interacts with ATF5; the ATF5:PCNT:polyglutamylated tubulin (PGT) tripartite unites the mother centriole and the pericentriolar material (PCM) in the centrosome (By similarity).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Cep290Q6A0782EBI-2290976,EBI-1811999

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi202052, 5 interactors
CORUMiP48725
IntActiP48725, 3 interactors
STRINGi10090.ENSMUSP00000001179

Structurei

3D structure databases

ProteinModelPortaliP48725
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1801 – 1822Interaction with CDK5RAP21 PublicationAdd BLAST22
Regioni2545 – 2810Interaction with NEK2By similarityAdd BLAST266
Regioni2758 – 2771Calmodulin-bindingBy similarityAdd BLAST14

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili127 – 343Sequence analysisAdd BLAST217
Coiled coili382 – 434Sequence analysisAdd BLAST53
Coiled coili468 – 527Sequence analysisAdd BLAST60
Coiled coili611 – 696Sequence analysisAdd BLAST86
Coiled coili727 – 787Sequence analysisAdd BLAST61
Coiled coili872 – 939Sequence analysisAdd BLAST68
Coiled coili1069 – 1383Sequence analysisAdd BLAST315
Coiled coili1429 – 1482Sequence analysisAdd BLAST54
Coiled coili1529 – 1593Sequence analysisAdd BLAST65
Coiled coili2211 – 2403Sequence analysisAdd BLAST193
Coiled coili2429 – 2590Sequence analysisAdd BLAST162

Domaini

Composed of a coiled-coil central region flanked by non-helical N- and C-terminals.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IJHT Eukaryota
ENOG4110FJZ LUCA
HOGENOMiHOG000168229
HOVERGENiHBG079443
InParanoidiP48725
KOiK16481
PhylomeDBiP48725

Family and domain databases

InterProiView protein in InterPro
IPR028745 AKAP9/Pericentrin
IPR019528 PACT_domain
PANTHERiPTHR44981 PTHR44981, 5 hits
PfamiView protein in Pfam
PF10495 PACT_coil_coil, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P48725-1) [UniParc]FASTAAdd to basket
Also known as: Pericentrin B, Pericentrin-360

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEDEQEQRRR KVEAGRAKLA NFRQRKTKGD CPNSKKKTAK RKGSAVHASV 
        60         70         80         90        100
QEEGSVATPN SELPQGGAVF ESPSCSNTLE GTRGASAAQE QEDCELDVTD 
       110        120        130        140        150
LQGQQQTQPP PPQTAHSLEL EALRLSLNNM HTAQLELTQA NLQKEKETAL 
       160        170        180        190        200
TELREMLNGR RAQELALLQS RQQCELELLR EQHAREKEEM ALRSGQEAAE 
       210        220        230        240        250
LKEKLRSEME KNAQTIETLK QDWESERELC LENLRQELSL KHQSEMEGLQ 
       260        270        280        290        300
SQFQKELSEQ KVELEKIFQA KHEAEVSLKN LEAQHQAAIK KLQEDLQSEH 
       310        320        330        340        350
CQYLQDLEQK FREKEKAKEL ELETLQASYE DLKAQSQEEI RLLWSQLESM 
       360        370        380        390        400
KTNREELNGS WDPVLAQASH LEELEHLRSG FAQQQQQERA QHESELEHLR 
       410        420        430        440        450
VYFEKKLKDA EKTYQEDLTV FQQRLQEARE DSLESTEISS SCVLPEETSG 
       460        470        480        490        500
REGKEPPDPL DLQLGQPKVQ ESLVEDCQVK LSKAEEKIQQ MKEEFQKKEA 
       510        520        530        540        550
EWELSREELK REAEERLASM FLELREKAES EKLSIISRFE HRESSMRHLQ 
       560        570        580        590        600
DQQAAQILDL ERSLMEQQGH LRQLEQELTR DDLLPCSQCG QEPAMAQEEK 
       610        620        630        640        650
NGALLREKED CALQLLMAQN RFLEERKEIM EKFAKEQDAF LRDAQEKHNH 
       660        670        680        690        700
ELQLLQQGHQ QQLLALRMEL ETKHRSELTE QLASSESRRQ ALLETHVAEL 
       710        720        730        740        750
QVKHNAEISA LEKRHLSNLD ELESCYVADV QTIRDEHQQA LELLRAELEE 
       760        770        780        790        800
QLQKKESCHR EMLTQELENL KRQHAEELQS VRDSLRMEMS AQHIENGKGP 
       810        820        830        840        850
AADLQGAHQQ DPAMALHNEG HLLVEDGDAV LRSVDAEGLL HQAGPQELGD 
       860        870        880        890        900
AHTVEMQKSQ AELAKPQELQ ASQDQVAQVR DKVFLLNREL EECRAELEQL 
       910        920        930        940        950
QQRRERENQE GTTLICMLRA DLELAQGEGK ALRDALRRLL DLFGDTLKAA 
       960        970        980        990       1000
VTLKSRISER AGLLLDHEDA ADTSDARLAA AALGDMWSDE GLLEIDRTLP 
      1010       1020       1030       1040       1050
EGAETSSVCE ISSHVCESFF ISPENTLDCE QPIRRVYQSL STAVEGLLEM 
      1060       1070       1080       1090       1100
ALDSSKQLEE ARQLHRCVER EFRHRNEEMA QAMQKQQELL ERLREESAAK 
      1110       1120       1130       1140       1150
DRLALELHTA KGLLEGFKVE KVDLQEALGK KEESEQQLIL ELEDLRKQLQ 
      1160       1170       1180       1190       1200
QAARELLTLK EEKSVLWNQK ETLTNEAKER EAALQEEVES LTRVQWESRK 
      1210       1220       1230       1240       1250
QSEKDRATLL SQMRVLESEL EDQLVQHRGC AQLAEEVATL KQQLAALDKH 
      1260       1270       1280       1290       1300
LRSQRQFMDD QAAEREHERE EFQQEIQRLE GQLRQAARPR PPGPRDSQCV 
      1310       1320       1330       1340       1350
QLDEEVELLQ EKLREKLDGF NELVIKKDFA DQQLLIQEEE IKRLEETNAS 
      1360       1370       1380       1390       1400
IQRQMVQLQE ELEKQKKSME ELKEKEILKQ ENMGDLLLTT VSRSGLDEAG 
      1410       1420       1430       1440       1450
CPMLPQGSSS RGPEAQPDVT ERALLQHENE VVHRRNSEID ELKSLIENLQ 
      1460       1470       1480       1490       1500
ENQRQLQKDK AEEIEQLHEV IEKLQSELSL MGPKVHEVSD PQAGSLHSEL 
      1510       1520       1530       1540       1550
ACLRGEGLGG QALRSELQAA QAAKEVFGQL LANQAHGHSQ ALEALQQRLQ 
      1560       1570       1580       1590       1600
DAEEVAARHL AELEHCVALR EAEVEAMASQ IQEFAATLKA KEAIIEQRDL 
      1610       1620       1630       1640       1650
EIDAVNKWKV SHSLELEAIL LALAHFRHAL EQQTCATPDE PPELRQLRVQ 
      1660       1670       1680       1690       1700
CARLSHQLQV LYRPFLKCRM QLDQHQPHVA SIGCANPCAD DELEQEGVSN 
      1710       1720       1730       1740       1750
RLALAPHSLA AQAKEELEDC PLGKANLMAQ VRQLQEELDH RVHSVASRDT 
      1760       1770       1780       1790       1800
NSETCKLQQP NLSENGPRNH CCNGEESKPS PPDDVLNIAK TTWDVIDIIK 
      1810       1820       1830       1840       1850
NQDLLVQVEM PDFPTQEKLT SQGGPFSSQA SGHSGSLLPE EAAEPQQDPV 
      1860       1870       1880       1890       1900
RALDLSSWSS PEVVRKDPSL EPQHSLPLTP GVGTVSLHSV DISPDWTDPL 
      1910       1920       1930       1940       1950
LQADVSGLLC YPGKSASGQA PLWAVAPSAG KHHAERTATE KDVEDFIVTS 
      1960       1970       1980       1990       2000
FDSQELLTSP SHELARRSDG SRKSDGPDIA MMLTLGSEGS ETPTTDLVAA 
      2010       2020       2030       2040       2050
AAAAVPFSRR FVQSPGAMKE KEIHAKQMKA LLQMVFDESH QILALSESQD 
      2060       2070       2080       2090       2100
PSSALNKGEP RDPLDGFPRD SQALSEVTTD KGEKESLETH LTWSEELLRA 
      2110       2120       2130       2140       2150
IQEVFAREQE KAELQPRPYG SNLGDYNSLV QRLEKVIQEQ GDPQKVQDHL 
      2160       2170       2180       2190       2200
CLSDRSSLLA EIQALRAQLR MTHLQNQEKL QQLCAALTST EARGSQREHQ 
      2210       2220       2230       2240       2250
LRRQVELLAY KVEQEKCIAN ELQKTLSKEQ ETASDVRKRL VVEQNAVQDL 
      2260       2270       2280       2290       2300
KSELHACKQE NTSLLESLDK VQQEVLRLRA VLDGKEKELK VVLEELESER 
      2310       2320       2330       2340       2350
GKGQALQAQQ EEQQLRYLQR EGQSSRALEE LKLSLEKQLA QNNQLCVALK 
      2360       2370       2380       2390       2400
HERAAKDNLQ KELQIEASRC EALLAQEKGQ LSELQKSLEA ERSRSLELSE 
      2410       2420       2430       2440       2450
ALQHERLLTE QLSRNSQEAC ARQETQVQHA LLRKLKAEKT RALELEAMLE 
      2460       2470       2480       2490       2500
KVQKQAAHTQ QQLEAQAQER CVELRREKER ELEIQRQRDE HKIEQLQRLV 
      2510       2520       2530       2540       2550
RELRWKEEVS GGNGPCRGSP GRGSLERDQF QEQQQELEKI RQQLLCAAGL 
      2560       2570       2580       2590       2600
LTSFTNHTVD RTIKDWTSSN EKAVSSLMRT LEELKSELSM PTSFQKKMTA 
      2610       2620       2630       2640       2650
ELQVQLMNEL LSDNDALTKA VGMATREKAE LCRTVSRLEK TLKHHTQKGC 
      2660       2670       2680       2690       2700
VLNRQSKSSL KQDGTDLQSS LRHSDPEWHS QTTSGDTNTC NIKMEKLYLH 
      2710       2720       2730       2740       2750
YLRAESFRKA LIYQKKYLLL LIGGFQDSEQ ETLSMIAHLG VFPSKADKKI 
      2760       2770       2780       2790       2800
TMSRPFTKFR TAVRVVIAVL RLRFLVKKWQ EVDRKGALVH PKSTRHGHRT 
      2810       2820       2830       2840       2850
SQRQRSPSGP RASLPTRDTS SGPTKASRHS PRSAAAGSPG KERSTSTPSS 
      2860       2870       2880       2890 
RLERSLTASQ DPEHSLTEYI HHLEMIQQRL GGLPPDSTQK SCHQKIKQ
Length:2,898
Mass (Da):329,465
Last modified:April 20, 2010 - v2
Checksum:i50E66D93F7E4E283
GO
Isoform 2 (identifier: P48725-2) [UniParc]FASTAAdd to basket
Also known as: Pericentrin S, Pericentrin-255

The sequence of this isoform differs from the canonical sequence as follows:
     1-916: Missing.

Show »
Length:1,982
Mass (Da):223,682
Checksum:i2781F4B76DB80818
GO
Isoform 3 (identifier: P48725-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     2044-2050: ALSESQD → GNSRLLF
     2501-2898: Missing.

Note: No experimental confirmation available.
Show »
Length:2,500
Mass (Da):284,589
Checksum:iF17397506865AAAE
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8VPV0F8VPV0_MOUSE
Pericentrin
Pcnt
2,916Annotation score:
A0A1W2P737A0A1W2P737_MOUSE
Pericentrin
Pcnt
2,898Annotation score:
A0A1W2P8A6A0A1W2P8A6_MOUSE
Pericentrin
Pcnt
156Annotation score:

Sequence cautioni

The sequence AAA17886 differs from that shown. Reason: Erroneous termination at position 1923. Translated as Trp.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti652Missing in AAA17886 (PubMed:8124707).Curated1
Sequence conflicti662Missing in AAA17886 (PubMed:8124707).Curated1
Sequence conflicti896 – 897EL → DV in AAA17886 (PubMed:8124707).Curated2
Sequence conflicti960R → G in AAA17886 (PubMed:8124707).Curated1
Sequence conflicti982A → P in AAA17886 (PubMed:8124707).Curated1
Sequence conflicti1022S → R in AAA17886 (PubMed:8124707).Curated1
Sequence conflicti1150Q → E in AAA17886 (PubMed:8124707).Curated1
Sequence conflicti1266 – 1267EH → DD in AAA17886 (PubMed:8124707).Curated2
Sequence conflicti1533N → D in AAA17886 (PubMed:8124707).Curated1
Sequence conflicti1548R → G in AAA17886 (PubMed:8124707).Curated1
Sequence conflicti1726N → G in AAA17886 (PubMed:8124707).Curated1
Sequence conflicti1736E → Q in AAA17886 (PubMed:8124707).Curated1
Sequence conflicti1772C → S in AAA17886 (PubMed:8124707).Curated1
Sequence conflicti2040H → L in AAA17886 (PubMed:8124707).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0389271 – 916Missing in isoform 2. 1 PublicationAdd BLAST916
Alternative sequenceiVSP_0389282044 – 2050ALSESQD → GNSRLLF in isoform 3. 1 Publication7
Alternative sequenceiVSP_0389292501 – 2898Missing in isoform 3. 1 PublicationAdd BLAST398

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U05823 mRNA Translation: AAA17886.1 Sequence problems.
AB207233 mRNA Translation: BAF36559.1
AB207234 mRNA Translation: BAF36560.1
PIRiA53188
RefSeqiNP_001269921.1, NM_001282992.1
NP_032813.3, NM_008787.3
UniGeneiMm.251794

Genome annotation databases

GeneIDi18541
KEGGimmu:18541
UCSCiuc007fuj.1 mouse [P48725-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U05823 mRNA Translation: AAA17886.1 Sequence problems.
AB207233 mRNA Translation: BAF36559.1
AB207234 mRNA Translation: BAF36560.1
PIRiA53188
RefSeqiNP_001269921.1, NM_001282992.1
NP_032813.3, NM_008787.3
UniGeneiMm.251794

3D structure databases

ProteinModelPortaliP48725
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202052, 5 interactors
CORUMiP48725
IntActiP48725, 3 interactors
STRINGi10090.ENSMUSP00000001179

PTM databases

iPTMnetiP48725
PhosphoSitePlusiP48725

Proteomic databases

EPDiP48725
MaxQBiP48725
PaxDbiP48725
PeptideAtlasiP48725
PRIDEiP48725

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi18541
KEGGimmu:18541
UCSCiuc007fuj.1 mouse [P48725-1]

Organism-specific databases

CTDi5116
MGIiMGI:102722 Pcnt

Phylogenomic databases

eggNOGiENOG410IJHT Eukaryota
ENOG4110FJZ LUCA
HOGENOMiHOG000168229
HOVERGENiHBG079443
InParanoidiP48725
KOiK16481
PhylomeDBiP48725

Miscellaneous databases

PROiPR:P48725
SOURCEiSearch...

Gene expression databases

CleanExiMM_PCNT

Family and domain databases

InterProiView protein in InterPro
IPR028745 AKAP9/Pericentrin
IPR019528 PACT_domain
PANTHERiPTHR44981 PTHR44981, 5 hits
PfamiView protein in Pfam
PF10495 PACT_coil_coil, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPCNT_MOUSE
AccessioniPrimary (citable) accession number: P48725
Secondary accession number(s): A0JBT0, A0JBT1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: April 20, 2010
Last modified: July 18, 2018
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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