UniProtKB - P48725 (PCNT_MOUSE)
Pericentrin
Pcnt
Functioni
GO - Molecular functioni
- calmodulin binding Source: UniProtKB-KW
- molecular adaptor activity Source: InterPro
GO - Biological processi
- brain morphogenesis Source: MGI
- camera-type eye development Source: MGI
- cerebellar cortex morphogenesis Source: MGI
- cilium assembly Source: MGI
- embryonic brain development Source: MGI
- embryonic heart tube development Source: MGI
- head development Source: MGI
- in utero embryonic development Source: MGI
- kidney development Source: MGI
- limb morphogenesis Source: MGI
- microtubule cytoskeleton organization Source: UniProtKB
- mitotic spindle organization Source: MGI
- multicellular organism growth Source: MGI
- negative regulation of apoptotic process Source: MGI
- neural precursor cell proliferation Source: MGI
- neuron migration Source: MGI
- olfactory bulb development Source: MGI
- positive regulation of intracellular protein transport Source: UniProtKB
- signal transduction Source: InterPro
- spindle organization Source: MGI
- vasculature development Source: MGI
Keywordsi
Molecular function | Calmodulin-binding |
Enzyme and pathway databases
Reactomei | R-MMU-2565942, Regulation of PLK1 Activity at G2/M Transition R-MMU-380259, Loss of Nlp from mitotic centrosomes R-MMU-380270, Recruitment of mitotic centrosome proteins and complexes R-MMU-380284, Loss of proteins required for interphase microtubule organization from the centrosome R-MMU-380320, Recruitment of NuMA to mitotic centrosomes R-MMU-5620912, Anchoring of the basal body to the plasma membrane R-MMU-8854518, AURKA Activation by TPX2 R-MMU-9646399, Aggrephagy |
Names & Taxonomyi
Protein namesi | Recommended name: Pericentrin |
Gene namesi | Name:Pcnt Synonyms:Pcnt2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:102722, Pcnt |
Subcellular locationi
Cytoskeleton
- centrosome By similarity
Note: Centrosomal at all stages of the cell cycle. Remains associated with centrosomes following microtubule depolymerization. Colocalized with DISC1 at the centrosome.By similarity
Cytoskeleton
- centriolar satellite Source: UniProtKB
- centriole Source: MGI
- centrosome Source: MGI
- ciliary basal body Source: MGI
- microtubule Source: UniProtKB-KW
- microtubule organizing center Source: MGI
- pericentriolar material Source: MGI
Extracellular region or secreted
- intercellular bridge Source: MGI
Other locations
- cytoplasm Source: UniProtKB-KW
- motile cilium Source: MGI
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, MicrotubulePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000058258 | 1 – 2898 | PericentrinAdd BLAST | 2898 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 44 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1022 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1437 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1828 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1859 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1860 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1959 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1987 | PhosphoserineBy similarity | 1 | |
Modified residuei | 2128 | PhosphoserineBy similarity | 1 | |
Modified residuei | 2865 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | P48725 |
jPOSTi | P48725 |
MaxQBi | P48725 |
PaxDbi | P48725 |
PeptideAtlasi | P48725 |
PRIDEi | P48725 |
PTM databases
iPTMneti | P48725 |
PhosphoSitePlusi | P48725 |
Expressioni
Tissue specificityi
Developmental stagei
Interactioni
Subunit structurei
Interacts with DISC1 and PCM1. Binds calmodulin.
Interacts with CEP131 (By similarity).
Interacts with CDK5RAP2; the interaction is leading to centrosomal localization of PCNT and CDK5RAP2 (PubMed:20471352).
Interacts with CHD3 (PubMed:17626165).
Interacts with CHD4; the interaction regulates centrosome integrity (PubMed:17626165).
Interacts with NEK2 (By similarity).
Interacts with CCDC13 (By similarity).
Interacts with CEP68 (By similarity).
Interacts with ATF5; the ATF5:PCNT:polyglutamylated tubulin (PGT) tripartite unites the mother centriole and the pericentriolar material (PCM) in the centrosome (By similarity).
By similarity2 PublicationsBinary interactionsi
P48725
With | #Exp. | IntAct |
---|---|---|
Cep290 [Q6A078] | 2 | EBI-2290976,EBI-1811999 |
GO - Molecular functioni
- calmodulin binding Source: UniProtKB-KW
Protein-protein interaction databases
BioGRIDi | 202052, 7 interactors |
CORUMi | P48725 |
IntActi | P48725, 3 interactors |
STRINGi | 10090.ENSMUSP00000001179 |
Miscellaneous databases
RNActi | P48725, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1801 – 1822 | Interaction with CDK5RAP21 PublicationAdd BLAST | 22 | |
Regioni | 2545 – 2810 | Interaction with NEK2By similarityAdd BLAST | 266 | |
Regioni | 2758 – 2771 | Calmodulin-bindingBy similarityAdd BLAST | 14 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 127 – 343 | Sequence analysisAdd BLAST | 217 | |
Coiled coili | 382 – 434 | Sequence analysisAdd BLAST | 53 | |
Coiled coili | 468 – 527 | Sequence analysisAdd BLAST | 60 | |
Coiled coili | 611 – 696 | Sequence analysisAdd BLAST | 86 | |
Coiled coili | 727 – 787 | Sequence analysisAdd BLAST | 61 | |
Coiled coili | 872 – 939 | Sequence analysisAdd BLAST | 68 | |
Coiled coili | 1069 – 1383 | Sequence analysisAdd BLAST | 315 | |
Coiled coili | 1429 – 1482 | Sequence analysisAdd BLAST | 54 | |
Coiled coili | 1529 – 1593 | Sequence analysisAdd BLAST | 65 | |
Coiled coili | 2211 – 2403 | Sequence analysisAdd BLAST | 193 | |
Coiled coili | 2429 – 2590 | Sequence analysisAdd BLAST | 162 |
Domaini
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG502QV16, Eukaryota |
InParanoidi | P48725 |
OrthoDBi | 8180at2759 |
PhylomeDBi | P48725 |
Family and domain databases
InterProi | View protein in InterPro IPR028745, AKAP9/Pericentrin IPR019528, PACT_domain |
PANTHERi | PTHR44981, PTHR44981, 2 hits |
Pfami | View protein in Pfam PF10495, PACT_coil_coil, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEDEQEQRRR KVEAGRAKLA NFRQRKTKGD CPNSKKKTAK RKGSAVHASV
60 70 80 90 100
QEEGSVATPN SELPQGGAVF ESPSCSNTLE GTRGASAAQE QEDCELDVTD
110 120 130 140 150
LQGQQQTQPP PPQTAHSLEL EALRLSLNNM HTAQLELTQA NLQKEKETAL
160 170 180 190 200
TELREMLNGR RAQELALLQS RQQCELELLR EQHAREKEEM ALRSGQEAAE
210 220 230 240 250
LKEKLRSEME KNAQTIETLK QDWESERELC LENLRQELSL KHQSEMEGLQ
260 270 280 290 300
SQFQKELSEQ KVELEKIFQA KHEAEVSLKN LEAQHQAAIK KLQEDLQSEH
310 320 330 340 350
CQYLQDLEQK FREKEKAKEL ELETLQASYE DLKAQSQEEI RLLWSQLESM
360 370 380 390 400
KTNREELNGS WDPVLAQASH LEELEHLRSG FAQQQQQERA QHESELEHLR
410 420 430 440 450
VYFEKKLKDA EKTYQEDLTV FQQRLQEARE DSLESTEISS SCVLPEETSG
460 470 480 490 500
REGKEPPDPL DLQLGQPKVQ ESLVEDCQVK LSKAEEKIQQ MKEEFQKKEA
510 520 530 540 550
EWELSREELK REAEERLASM FLELREKAES EKLSIISRFE HRESSMRHLQ
560 570 580 590 600
DQQAAQILDL ERSLMEQQGH LRQLEQELTR DDLLPCSQCG QEPAMAQEEK
610 620 630 640 650
NGALLREKED CALQLLMAQN RFLEERKEIM EKFAKEQDAF LRDAQEKHNH
660 670 680 690 700
ELQLLQQGHQ QQLLALRMEL ETKHRSELTE QLASSESRRQ ALLETHVAEL
710 720 730 740 750
QVKHNAEISA LEKRHLSNLD ELESCYVADV QTIRDEHQQA LELLRAELEE
760 770 780 790 800
QLQKKESCHR EMLTQELENL KRQHAEELQS VRDSLRMEMS AQHIENGKGP
810 820 830 840 850
AADLQGAHQQ DPAMALHNEG HLLVEDGDAV LRSVDAEGLL HQAGPQELGD
860 870 880 890 900
AHTVEMQKSQ AELAKPQELQ ASQDQVAQVR DKVFLLNREL EECRAELEQL
910 920 930 940 950
QQRRERENQE GTTLICMLRA DLELAQGEGK ALRDALRRLL DLFGDTLKAA
960 970 980 990 1000
VTLKSRISER AGLLLDHEDA ADTSDARLAA AALGDMWSDE GLLEIDRTLP
1010 1020 1030 1040 1050
EGAETSSVCE ISSHVCESFF ISPENTLDCE QPIRRVYQSL STAVEGLLEM
1060 1070 1080 1090 1100
ALDSSKQLEE ARQLHRCVER EFRHRNEEMA QAMQKQQELL ERLREESAAK
1110 1120 1130 1140 1150
DRLALELHTA KGLLEGFKVE KVDLQEALGK KEESEQQLIL ELEDLRKQLQ
1160 1170 1180 1190 1200
QAARELLTLK EEKSVLWNQK ETLTNEAKER EAALQEEVES LTRVQWESRK
1210 1220 1230 1240 1250
QSEKDRATLL SQMRVLESEL EDQLVQHRGC AQLAEEVATL KQQLAALDKH
1260 1270 1280 1290 1300
LRSQRQFMDD QAAEREHERE EFQQEIQRLE GQLRQAARPR PPGPRDSQCV
1310 1320 1330 1340 1350
QLDEEVELLQ EKLREKLDGF NELVIKKDFA DQQLLIQEEE IKRLEETNAS
1360 1370 1380 1390 1400
IQRQMVQLQE ELEKQKKSME ELKEKEILKQ ENMGDLLLTT VSRSGLDEAG
1410 1420 1430 1440 1450
CPMLPQGSSS RGPEAQPDVT ERALLQHENE VVHRRNSEID ELKSLIENLQ
1460 1470 1480 1490 1500
ENQRQLQKDK AEEIEQLHEV IEKLQSELSL MGPKVHEVSD PQAGSLHSEL
1510 1520 1530 1540 1550
ACLRGEGLGG QALRSELQAA QAAKEVFGQL LANQAHGHSQ ALEALQQRLQ
1560 1570 1580 1590 1600
DAEEVAARHL AELEHCVALR EAEVEAMASQ IQEFAATLKA KEAIIEQRDL
1610 1620 1630 1640 1650
EIDAVNKWKV SHSLELEAIL LALAHFRHAL EQQTCATPDE PPELRQLRVQ
1660 1670 1680 1690 1700
CARLSHQLQV LYRPFLKCRM QLDQHQPHVA SIGCANPCAD DELEQEGVSN
1710 1720 1730 1740 1750
RLALAPHSLA AQAKEELEDC PLGKANLMAQ VRQLQEELDH RVHSVASRDT
1760 1770 1780 1790 1800
NSETCKLQQP NLSENGPRNH CCNGEESKPS PPDDVLNIAK TTWDVIDIIK
1810 1820 1830 1840 1850
NQDLLVQVEM PDFPTQEKLT SQGGPFSSQA SGHSGSLLPE EAAEPQQDPV
1860 1870 1880 1890 1900
RALDLSSWSS PEVVRKDPSL EPQHSLPLTP GVGTVSLHSV DISPDWTDPL
1910 1920 1930 1940 1950
LQADVSGLLC YPGKSASGQA PLWAVAPSAG KHHAERTATE KDVEDFIVTS
1960 1970 1980 1990 2000
FDSQELLTSP SHELARRSDG SRKSDGPDIA MMLTLGSEGS ETPTTDLVAA
2010 2020 2030 2040 2050
AAAAVPFSRR FVQSPGAMKE KEIHAKQMKA LLQMVFDESH QILALSESQD
2060 2070 2080 2090 2100
PSSALNKGEP RDPLDGFPRD SQALSEVTTD KGEKESLETH LTWSEELLRA
2110 2120 2130 2140 2150
IQEVFAREQE KAELQPRPYG SNLGDYNSLV QRLEKVIQEQ GDPQKVQDHL
2160 2170 2180 2190 2200
CLSDRSSLLA EIQALRAQLR MTHLQNQEKL QQLCAALTST EARGSQREHQ
2210 2220 2230 2240 2250
LRRQVELLAY KVEQEKCIAN ELQKTLSKEQ ETASDVRKRL VVEQNAVQDL
2260 2270 2280 2290 2300
KSELHACKQE NTSLLESLDK VQQEVLRLRA VLDGKEKELK VVLEELESER
2310 2320 2330 2340 2350
GKGQALQAQQ EEQQLRYLQR EGQSSRALEE LKLSLEKQLA QNNQLCVALK
2360 2370 2380 2390 2400
HERAAKDNLQ KELQIEASRC EALLAQEKGQ LSELQKSLEA ERSRSLELSE
2410 2420 2430 2440 2450
ALQHERLLTE QLSRNSQEAC ARQETQVQHA LLRKLKAEKT RALELEAMLE
2460 2470 2480 2490 2500
KVQKQAAHTQ QQLEAQAQER CVELRREKER ELEIQRQRDE HKIEQLQRLV
2510 2520 2530 2540 2550
RELRWKEEVS GGNGPCRGSP GRGSLERDQF QEQQQELEKI RQQLLCAAGL
2560 2570 2580 2590 2600
LTSFTNHTVD RTIKDWTSSN EKAVSSLMRT LEELKSELSM PTSFQKKMTA
2610 2620 2630 2640 2650
ELQVQLMNEL LSDNDALTKA VGMATREKAE LCRTVSRLEK TLKHHTQKGC
2660 2670 2680 2690 2700
VLNRQSKSSL KQDGTDLQSS LRHSDPEWHS QTTSGDTNTC NIKMEKLYLH
2710 2720 2730 2740 2750
YLRAESFRKA LIYQKKYLLL LIGGFQDSEQ ETLSMIAHLG VFPSKADKKI
2760 2770 2780 2790 2800
TMSRPFTKFR TAVRVVIAVL RLRFLVKKWQ EVDRKGALVH PKSTRHGHRT
2810 2820 2830 2840 2850
SQRQRSPSGP RASLPTRDTS SGPTKASRHS PRSAAAGSPG KERSTSTPSS
2860 2870 2880 2890
RLERSLTASQ DPEHSLTEYI HHLEMIQQRL GGLPPDSTQK SCHQKIKQ
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF8VPV0 | F8VPV0_MOUSE | Pericentrin | Pcnt | 2,916 | Annotation score: | ||
A0A1W2P737 | A0A1W2P737_MOUSE | Pericentrin | Pcnt | 2,898 | Annotation score: | ||
A0A1W2P8A6 | A0A1W2P8A6_MOUSE | Pericentrin | Pcnt | 156 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 652 | Missing in AAA17886 (PubMed:8124707).Curated | 1 | |
Sequence conflicti | 662 | Missing in AAA17886 (PubMed:8124707).Curated | 1 | |
Sequence conflicti | 896 – 897 | EL → DV in AAA17886 (PubMed:8124707).Curated | 2 | |
Sequence conflicti | 960 | R → G in AAA17886 (PubMed:8124707).Curated | 1 | |
Sequence conflicti | 982 | A → P in AAA17886 (PubMed:8124707).Curated | 1 | |
Sequence conflicti | 1022 | S → R in AAA17886 (PubMed:8124707).Curated | 1 | |
Sequence conflicti | 1150 | Q → E in AAA17886 (PubMed:8124707).Curated | 1 | |
Sequence conflicti | 1266 – 1267 | EH → DD in AAA17886 (PubMed:8124707).Curated | 2 | |
Sequence conflicti | 1533 | N → D in AAA17886 (PubMed:8124707).Curated | 1 | |
Sequence conflicti | 1548 | R → G in AAA17886 (PubMed:8124707).Curated | 1 | |
Sequence conflicti | 1726 | N → G in AAA17886 (PubMed:8124707).Curated | 1 | |
Sequence conflicti | 1736 | E → Q in AAA17886 (PubMed:8124707).Curated | 1 | |
Sequence conflicti | 1772 | C → S in AAA17886 (PubMed:8124707).Curated | 1 | |
Sequence conflicti | 2040 | H → L in AAA17886 (PubMed:8124707).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_038927 | 1 – 916 | Missing in isoform 2. 1 PublicationAdd BLAST | 916 | |
Alternative sequenceiVSP_038928 | 2044 – 2050 | ALSESQD → GNSRLLF in isoform 3. 1 Publication | 7 | |
Alternative sequenceiVSP_038929 | 2501 – 2898 | Missing in isoform 3. 1 PublicationAdd BLAST | 398 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U05823 mRNA Translation: AAA17886.1 Sequence problems. AB207233 mRNA Translation: BAF36559.1 AB207234 mRNA Translation: BAF36560.1 |
CCDSi | CCDS88027.1 [P48725-1] |
PIRi | A53188 |
RefSeqi | NP_001269921.1, NM_001282992.1 NP_032813.3, NM_008787.3 |
Genome annotation databases
GeneIDi | 18541 |
KEGGi | mmu:18541 |
UCSCi | uc007fuj.1, mouse [P48725-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U05823 mRNA Translation: AAA17886.1 Sequence problems. AB207233 mRNA Translation: BAF36559.1 AB207234 mRNA Translation: BAF36560.1 |
CCDSi | CCDS88027.1 [P48725-1] |
PIRi | A53188 |
RefSeqi | NP_001269921.1, NM_001282992.1 NP_032813.3, NM_008787.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 202052, 7 interactors |
CORUMi | P48725 |
IntActi | P48725, 3 interactors |
STRINGi | 10090.ENSMUSP00000001179 |
PTM databases
iPTMneti | P48725 |
PhosphoSitePlusi | P48725 |
Proteomic databases
EPDi | P48725 |
jPOSTi | P48725 |
MaxQBi | P48725 |
PaxDbi | P48725 |
PeptideAtlasi | P48725 |
PRIDEi | P48725 |
Genome annotation databases
GeneIDi | 18541 |
KEGGi | mmu:18541 |
UCSCi | uc007fuj.1, mouse [P48725-1] |
Organism-specific databases
CTDi | 5116 |
MGIi | MGI:102722, Pcnt |
Phylogenomic databases
eggNOGi | ENOG502QV16, Eukaryota |
InParanoidi | P48725 |
OrthoDBi | 8180at2759 |
PhylomeDBi | P48725 |
Enzyme and pathway databases
Reactomei | R-MMU-2565942, Regulation of PLK1 Activity at G2/M Transition R-MMU-380259, Loss of Nlp from mitotic centrosomes R-MMU-380270, Recruitment of mitotic centrosome proteins and complexes R-MMU-380284, Loss of proteins required for interphase microtubule organization from the centrosome R-MMU-380320, Recruitment of NuMA to mitotic centrosomes R-MMU-5620912, Anchoring of the basal body to the plasma membrane R-MMU-8854518, AURKA Activation by TPX2 R-MMU-9646399, Aggrephagy |
Miscellaneous databases
BioGRID-ORCSi | 18541, 4 hits in 17 CRISPR screens |
ChiTaRSi | Pcnt, mouse |
PROi | PR:P48725 |
RNActi | P48725, protein |
SOURCEi | Search... |
Family and domain databases
InterProi | View protein in InterPro IPR028745, AKAP9/Pericentrin IPR019528, PACT_domain |
PANTHERi | PTHR44981, PTHR44981, 2 hits |
Pfami | View protein in Pfam PF10495, PACT_coil_coil, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PCNT_MOUSE | |
Accessioni | P48725Primary (citable) accession number: P48725 Secondary accession number(s): A0JBT0, A0JBT1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1996 |
Last sequence update: | April 20, 2010 | |
Last modified: | December 2, 2020 | |
This is version 143 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot