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Protein

Stress-70 protein, mitochondrial

Gene

Hspa9

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Chaperone protein which plays an important role in mitochondrial iron-sulfur cluster (ISC) biogenesis. Interacts with and stabilizes ISC cluster assembly proteins FXN, NFU1, NFS1 and ISCU. Regulates erythropoiesis via stabilization of ISC assembly. May play a role in the control of cell proliferation and cellular aging.By similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • chaperone binding Source: RGD
  • fibroblast growth factor binding Source: RGD
  • heat shock protein binding Source: RGD
  • unfolded protein binding Source: InterPro

GO - Biological processi

  • cellular response to interleukin-1 Source: RGD
  • erythrocyte differentiation Source: UniProtKB
  • iron-sulfur cluster assembly Source: UniProtKB
  • negative regulation of cell death Source: RGD
  • negative regulation of erythrocyte differentiation Source: UniProtKB
  • negative regulation of hematopoietic stem cell differentiation Source: UniProtKB
  • negative regulation of hemopoiesis Source: UniProtKB
  • protein autophosphorylation Source: RGD
  • protein folding Source: InterPro
  • regulation of erythrocyte differentiation Source: UniProtKB
  • response to folic acid Source: RGD
  • response to thyroxine Source: RGD
  • response to toxic substance Source: RGD

Keywordsi

Molecular functionChaperone
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Stress-70 protein, mitochondrial
Alternative name(s):
75 kDa glucose-regulated protein
Short name:
GRP-75
Heat shock 70 kDa protein 9
Mortalin
Peptide-binding protein 74
Short name:
PBP74
mtHSP70
Gene namesi
Name:Hspa9
Synonyms:Grp75, Hspa9a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1311806 Hspa9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 46MitochondrionBy similarityAdd BLAST46
ChainiPRO_000001356547 – 679Stress-70 protein, mitochondrialAdd BLAST633

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei76N6-acetyllysineBy similarity1
Modified residuei87PhosphothreonineBy similarity1
Modified residuei135N6-acetyllysine; alternateBy similarity1
Modified residuei135N6-succinyllysine; alternateBy similarity1
Modified residuei138N6-acetyllysine; alternateBy similarity1
Modified residuei138N6-succinyllysine; alternateBy similarity1
Modified residuei143N6-acetyllysineBy similarity1
Modified residuei206N6-acetyllysine; alternateBy similarity1
Modified residuei206N6-malonyllysine; alternateBy similarity1
Modified residuei206N6-succinyllysine; alternateBy similarity1
Modified residuei234N6-acetyllysineBy similarity1
Modified residuei288N6-acetyllysineBy similarity1
Modified residuei300N6-acetyllysine; alternateBy similarity1
Modified residuei300N6-succinyllysine; alternateBy similarity1
Modified residuei360N6-acetyllysine; alternateBy similarity1
Modified residuei360N6-succinyllysine; alternateBy similarity1
Modified residuei368N6-succinyllysineBy similarity1
Modified residuei394N6-succinyllysineBy similarity1
Modified residuei408PhosphoserineBy similarity1
Modified residuei513Omega-N-methylarginineBy similarity1
Modified residuei567N6-acetyllysine; alternateBy similarity1
Modified residuei567N6-succinyllysine; alternateBy similarity1
Modified residuei600N6-acetyllysine; alternateBy similarity1
Modified residuei600N6-succinyllysine; alternateBy similarity1
Modified residuei610N6-succinyllysineBy similarity1
Modified residuei612N6-acetyllysineBy similarity1
Modified residuei646N6-acetyllysine; alternateBy similarity1
Modified residuei646N6-succinyllysine; alternateBy similarity1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

PaxDbiP48721
PRIDEiP48721

2D gel databases

World-2DPAGEi0004:P48721

PTM databases

CarbonylDBiP48721
iPTMnetiP48721
PhosphoSitePlusiP48721
SwissPalmiP48721

Interactioni

Subunit structurei

Interacts strongly with the intermediate form of FXN and weakly with its mature form. Interacts with HSCB. Associates with the mitochondrial contact site and cristae organizing system (MICOS) complex, composed of at least MINOS1/MIC10, CHCHD3/MIC19, CHCHD6/MIC25, APOOL/MIC27, IMMT/MIC60, APOO/MIC23/MIC26 and QIL1/MIC13. This complex was also known under the names MINOS or MitOS complex. The MICOS complex associates with mitochondrial outer membrane proteins SAMM50, MTX1, MTX2 and DNAJC11, mitochondrial inner membrane protein TMEM11 and with HSPA9. Interacts with DNLZ, the interaction is required to prevent self-aggregation. Interacts with TESPA1. Interacts with PDPN. Interacts with NFU1, NFS1 and ISCU.By similarity

GO - Molecular functioni

  • chaperone binding Source: RGD
  • fibroblast growth factor binding Source: RGD
  • heat shock protein binding Source: RGD
  • unfolded protein binding Source: InterPro

Protein-protein interaction databases

IntActiP48721, 4 interactors
MINTiP48721
STRINGi10116.ENSRNOP00000026696

Structurei

3D structure databases

ProteinModelPortaliP48721
SMRiP48721
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 432Interaction with NFS1By similarityAdd BLAST432
Regioni432 – 679Interaction with FXN and ISCUBy similarityAdd BLAST248

Sequence similaritiesi

Belongs to the heat shock protein 70 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0102 Eukaryota
COG0443 LUCA
HOGENOMiHOG000228136
HOVERGENiHBG051845
InParanoidiP48721
PhylomeDBiP48721

Family and domain databases

Gene3Di1.20.1270.10, 1 hit
2.60.34.10, 1 hit
HAMAPiMF_00332 DnaK, 1 hit
InterProiView protein in InterPro
IPR012725 Chaperone_DnaK
IPR018181 Heat_shock_70_CS
IPR029048 HSP70_C_sf
IPR029047 HSP70_peptide-bd_sf
IPR013126 Hsp_70_fam
PANTHERiPTHR19375 PTHR19375, 1 hit
PfamiView protein in Pfam
PF00012 HSP70, 1 hit
PRINTSiPR00301 HEATSHOCK70
SUPFAMiSSF100920 SSF100920, 1 hit
TIGRFAMsiTIGR02350 prok_dnaK, 1 hit
PROSITEiView protein in PROSITE
PS00297 HSP70_1, 1 hit
PS00329 HSP70_2, 1 hit
PS01036 HSP70_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48721-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISASRAAAA RLVGTTASRS PAAARHQDGW NGLSHEVFRF VSRRDYASEA
60 70 80 90 100
IKGAVVGIDL GTTNSCVAVM EGKQAKVLEN AEGARTTPSV VAFTPDGERL
110 120 130 140 150
VGMPAKRQAV TNPNNTFYAT KRLIGRRYDD PEVQKDTKNV PFKIVRASNG
160 170 180 190 200
DAWVEAHGKL YSPSQIGAFV LMKMKETAEN YLGHTAKNAV ITVPAYFNDS
210 220 230 240 250
QRQATKDAGQ ISGLNVLRVI NEPTAAALAY GLDKSEDKVI AVYDLGGGTF
260 270 280 290 300
DISILEIQKG VFEVKSTNGD TFLGGEDFDQ ALLRHIVKEF KRETGVDLTK
310 320 330 340 350
DNMALQRVRE AAEKAKCELS SSVQTDINLP YLTMDASGPK HLNMKLTRAQ
360 370 380 390 400
FEGIVTDLIK RTIAPCQKAM QDREVSKSDI GEVILVGGMT RMPKVQQTVQ
410 420 430 440 450
DLFGRAPSKA VNPDEAVAIG AAIQGGVLAG DVTDVLLLDV TPLSLGIETL
460 470 480 490 500
GGVFTKLINR NTTIPTKKSQ VFSTAADGQT QVEIKVCQGE REMAGDNKLL
510 520 530 540 550
GQFTLIGIPP APRGVPQIEV TFDIDANGIV HVSAKDKGTG REQQIVIQSS
560 570 580 590 600
GGLSKDDIEN MVKNAEKYAE EDRRKKERVE AVNMAEGIIH DTETKMEEFK
610 620 630 640 650
DQLPADECNK LKEEISKMRE LLARKDSETG ENIRQAASSL QQASLKLFEM
660 670
AYKKMASERE GSGSSSTGEQ KEDQKEEKQ
Length:679
Mass (Da):73,858
Last modified:October 17, 2006 - v3
Checksum:i4616EDE5307691A4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti37V → A in AAB34982 (PubMed:7629893).Curated1
Sequence conflicti81A → S in AAB33049 (PubMed:7811387).Curated1
Sequence conflicti373R → A in AAB34982 (PubMed:7629893).Curated1
Sequence conflicti487C → T AA sequence (PubMed:2372296).Curated1
Sequence conflicti493M → Q AA sequence (PubMed:2372296).Curated1
Sequence conflicti589I → V in AAB33049 (PubMed:7811387).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S75280 mRNA Translation: AAB33049.1
S78556 mRNA Translation: AAB34982.1
PIRiI56581
UniGeneiRn.7535

Genome annotation databases

UCSCiRGD:1311806 rat

Similar proteinsi

Entry informationi

Entry nameiGRP75_RAT
AccessioniPrimary (citable) accession number: P48721
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 17, 2006
Last modified: June 20, 2018
This is version 141 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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