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Protein

Retinal dehydrogenase 1

Gene

ALDH1A1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Can convert/oxidize retinaldehyde to retinoic acid. Binds free retinal and cellular retinol-binding protein-bound retinal. May have a broader specificity and oxidize other aldehydes in vivo.1 Publication

Catalytic activityi

Retinal + NAD+ + H2O = retinoate + NADH.1 Publication

Kineticsi

Has a much higher catalytic efficiency toward all-trans retinal.1 Publication
  1. KM=9 µM for all-trans retinal1 Publication
  2. KM=600 µM for propionaldehyde1 Publication
  1. Vmax=106 pmol/min/mg enzyme with all-trans retinal1 Publication
  2. Vmax=4650 pmol/min/mg enzyme with all-trans propionaldehyde1 Publication

Pathwayi: retinol metabolism

This protein is involved in the pathway retinol metabolism, which is part of Cofactor metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway retinol metabolism and in Cofactor metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei170Transition state stabilizerBy similarity1
Active sitei269Proton acceptorPROSITE-ProRule annotation1
Active sitei303NucleophilePROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi167 – 170NADBy similarity4
Nucleotide bindingi193 – 196NADBy similarity4
Nucleotide bindingi226 – 227NADBy similarity2
Nucleotide bindingi246 – 247NADBy similarity2
Nucleotide bindingi269 – 271NADBy similarity3
Nucleotide bindingi349 – 353NADBy similarity5
Nucleotide bindingi400 – 402NADBy similarity3

GO - Molecular functioni

  • aldehyde dehydrogenase (NAD) activity Source: UniProtKB
  • benzaldehyde dehydrogenase (NAD+) activity Source: GO_Central
  • NAD binding Source: CAFA
  • retinal dehydrogenase activity Source: UniProtKB

GO - Biological processi

  • oxidation-reduction process Source: UniProtKB
  • retinoid metabolic process Source: UniProtKB
  • retinol metabolic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionOxidoreductase
LigandNAD

Enzyme and pathway databases

ReactomeiR-BTA-5365859 RA biosynthesis pathway
R-BTA-70350 Fructose catabolism
R-BTA-71384 Ethanol oxidation
UniPathwayiUPA00912

Names & Taxonomyi

Protein namesi
Recommended name:
Retinal dehydrogenase 1Curated (EC:1.2.1.-1 Publication, EC:1.2.1.361 Publication)
Short name:
RALDH 1Curated
Short name:
RalDH1Curated
Alternative name(s):
Aldehyde dehydrogenase, cytosolicCurated
Gene namesi
Name:ALDH1A1By similarity
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 8

Organism-specific databases

VGNCiVGNC:25808 ALDH1A1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000564132 – 501Retinal dehydrogenase 1Add BLAST500

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei91N6-acetyllysineBy similarity1
Modified residuei128N6-acetyllysineBy similarity1
Modified residuei252N6-acetyllysineBy similarity1
Modified residuei337PhosphothreonineBy similarity1
Modified residuei353N6-acetyllysineBy similarity1
Modified residuei367N6-acetyllysineBy similarity1
Modified residuei410N6-acetyllysineBy similarity1
Modified residuei413PhosphoserineBy similarity1
Modified residuei419N6-acetyllysineBy similarity1
Modified residuei495N6-acetyllysineBy similarity1

Post-translational modificationi

The N-terminus is blocked most probably by acetylation.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP48644
PeptideAtlasiP48644
PRIDEiP48644

Expressioni

Tissue specificityi

Expressed in muscle, liver, small intestine, kidney, brain, lung, heart but not detected in erythrocytes (at protein level).1 Publication

Gene expression databases

BgeeiENSBTAG00000008103

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000010661

Structurei

3D structure databases

ProteinModelPortaliP48644
SMRiP48644
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family.Curated

Phylogenomic databases

eggNOGiKOG2450 Eukaryota
COG1012 LUCA
GeneTreeiENSGT00760000118999
HOGENOMiHOG000271505
HOVERGENiHBG000097
InParanoidiP48644
KOiK07249
OMAiGEWVKPV
OrthoDBiEOG091G05E8
TreeFamiTF300455

Family and domain databases

Gene3Di3.40.309.10, 1 hit
3.40.605.10, 2 hits
InterProiView protein in InterPro
IPR016161 Ald_DH/histidinol_DH
IPR016163 Ald_DH_C
IPR016160 Ald_DH_CS_CYS
IPR029510 Ald_DH_CS_GLU
IPR016162 Ald_DH_N
IPR015590 Aldehyde_DH_dom
PfamiView protein in Pfam
PF00171 Aldedh, 1 hit
SUPFAMiSSF53720 SSF53720, 1 hit
PROSITEiView protein in PROSITE
PS00070 ALDEHYDE_DEHYDR_CYS, 1 hit
PS00687 ALDEHYDE_DEHYDR_GLU, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48644-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSAMPDVP APLTNLQFKY TKIFINNEWH SSVSGKKFPV FNPATEEKLC
60 70 80 90 100
EVEEGDKEDV DKAVKAARQA FQIGSPWRTM DASERGRLLN KLADLIERDH
110 120 130 140 150
LLLATMEAMN GGKLFSNAYL MDLGGCIKTL RYCAGWADKI QGRTIPMDGN
160 170 180 190 200
FFTYTRSEPV GVCGQIIPWN FPLLMFLWKI GPALSCGNTV VVKPAEQTPL
210 220 230 240 250
TALHMGSLIK EAGFPPGVVN IVPGYGPTAG AAISSHMDVD KVAFTGSTEV
260 270 280 290 300
GKLIKEAAGK SNLKRVSLEL GGKSPCIVFA DADLDNAVEF AHQGVFYHQG
310 320 330 340 350
QCCIAASRLF VEESIYDEFV RRSVERAKKY VLGNPLTPGV SQGPQIDKEQ
360 370 380 390 400
YEKILDLIES GKKEGAKLEC GGGPWGNKGY FIQPTVFSDV TDDMRIAKEE
410 420 430 440 450
IFGPVQQIMK FKSLDDVIKR ANNTFYGLSA GIFTNDIDKA ITVSSALQSG
460 470 480 490 500
TVWVNCYSVV SAQCPFGGFK MSGNGRELGE YGFHEYTEVK TVTIKISQKN

S
Length:501
Mass (Da):54,806
Last modified:November 13, 2007 - v3
Checksum:iC9025D7034BC7F95
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti67A → V in AAA74234 (PubMed:7786847).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36128 mRNA Translation: AAA74234.1
BT030667 mRNA Translation: ABS44983.1
BC105193 mRNA Translation: AAI05194.1
RefSeqiNP_776664.1, NM_174239.2
UniGeneiBt.4732

Genome annotation databases

EnsembliENSBTAT00000010661; ENSBTAP00000010661; ENSBTAG00000008103
GeneIDi281615
KEGGibta:281615

Similar proteinsi

Entry informationi

Entry nameiAL1A1_BOVIN
AccessioniPrimary (citable) accession number: P48644
Secondary accession number(s): A7E3P6, Q3MHL5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: November 13, 2007
Last modified: March 28, 2018
This is version 124 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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