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Protein

T-complex protein 1 subunit epsilon

Gene

CCT5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.1 Publication

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • beta-tubulin binding Source: UniProtKB
  • G-protein beta-subunit binding Source: UniProtKB
  • mRNA 3'-UTR binding Source: CAFA
  • mRNA 5'-UTR binding Source: CAFA
  • protein binding involved in protein folding Source: GO_Central
  • unfolded protein binding Source: GO_Central

GO - Biological processi

  • 'de novo' protein folding Source: GO_Central
  • binding of sperm to zona pellucida Source: Ensembl
  • chaperone-mediated protein folding Source: GO_Central
  • positive regulation of establishment of protein localization to telomere Source: BHF-UCL
  • positive regulation of protein localization to Cajal body Source: BHF-UCL
  • positive regulation of telomerase RNA localization to Cajal body Source: BHF-UCL
  • positive regulation of telomere maintenance via telomerase Source: BHF-UCL
  • protein folding Source: Reactome
  • protein stabilization Source: BHF-UCL
  • response to virus Source: UniProtKB
  • toxin transport Source: Ensembl

Keywordsi

Molecular functionChaperone
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-389957 Prefoldin mediated transfer of substrate to CCT/TriC
R-HSA-389960 Formation of tubulin folding intermediates by CCT/TriC
R-HSA-390450 Folding of actin by CCT/TriC
R-HSA-390471 Association of TriC/CCT with target proteins during biosynthesis
R-HSA-5620922 BBSome-mediated cargo-targeting to cilium
R-HSA-6814122 Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding

Names & Taxonomyi

Protein namesi
Recommended name:
T-complex protein 1 subunit epsilon
Short name:
TCP-1-epsilon
Alternative name(s):
CCT-epsilon
Gene namesi
Name:CCT5
Synonyms:CCTE, KIAA0098
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000150753.11
HGNCiHGNC:1618 CCT5
MIMi610150 gene
neXtProtiNX_P48643

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Involvement in diseasei

Neuropathy, hereditary sensory, with spastic paraplegia, autosomal recessive (HSNSP)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA disease characterized by spastic paraplegia and progressive distal sensory neuropathy leading to mutilating ulcerations of the upper and lower limbs.
See also OMIM:256840
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_030658147H → R in HSNSP. 1 PublicationCorresponds to variant dbSNP:rs118203986EnsemblClinVar.1

Keywords - Diseasei

Disease mutation, Neuropathy

Organism-specific databases

DisGeNETi22948
MalaCardsiCCT5
MIMi256840 phenotype
OpenTargetsiENSG00000150753
Orphaneti139578 Hereditary sensory and autonomic neuropathy with spastic paraplegia
PharmGKBiPA26182

Polymorphism and mutation databases

BioMutaiCCT5
DMDMi1351211

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources2 Publications
ChainiPRO_00001283462 – 541T-complex protein 1 subunit epsilonAdd BLAST540

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources2 Publications1
Cross-linki20Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei26PhosphoserineCombined sources1
Cross-linki210Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki214Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki265Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki275Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki279Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei346PhosphoserineCombined sources1
Cross-linki392Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei539PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP48643
PaxDbiP48643
PeptideAtlasiP48643
PRIDEiP48643
ProteomicsDBi55918

2D gel databases

OGPiP48643
REPRODUCTION-2DPAGEiIPI00010720
SWISS-2DPAGEiP48643

PTM databases

iPTMnetiP48643
PhosphoSitePlusiP48643
SwissPalmiP48643

Expressioni

Inductioni

Down-regulated in response to enterovirus 71 (EV71) infection (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000150753
CleanExiHS_CCT5
ExpressionAtlasiP48643 baseline and differential
GenevisibleiP48643 HS

Organism-specific databases

HPAiCAB006271
HPA002238
HPA005958

Interactioni

Subunit structurei

Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter. Interacts with PACRG. Component of the BBS/CCT complex composed at least of MKKS, BBS10, BBS12, TCP1, CCT2, CCT3, CCT4, CCT5 AND CCT8. Interacts with DNAAF4 (By similarity).By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

  • beta-tubulin binding Source: UniProtKB
  • G-protein beta-subunit binding Source: UniProtKB
  • protein binding involved in protein folding Source: GO_Central
  • unfolded protein binding Source: GO_Central

Protein-protein interaction databases

BioGridi116603, 219 interactors
CORUMiP48643
DIPiDIP-31181N
IntActiP48643, 132 interactors
MINTiP48643
STRINGi9606.ENSP00000280326

Structurei

Secondary structure

1541
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi32 – 49Combined sources18
Helixi74 – 80Combined sources7
Helixi86 – 102Combined sources17
Helixi107 – 125Combined sources19
Helixi130 – 151Combined sources22
Beta strandi158 – 161Combined sources4
Helixi164 – 169Combined sources6
Helixi183 – 195Combined sources13
Beta strandi200 – 202Combined sources3
Beta strandi208 – 213Combined sources6
Helixi219 – 221Combined sources3
Beta strandi226 – 230Combined sources5
Beta strandi241 – 246Combined sources6
Beta strandi248 – 251Combined sources4
Helixi271 – 293Combined sources23
Beta strandi298 – 301Combined sources4
Helixi308 – 315Combined sources8
Turni316 – 318Combined sources3
Turni328 – 330Combined sources3
Helixi331 – 336Combined sources6
Helixi350 – 352Combined sources3
Beta strandi356 – 364Combined sources9
Beta strandi367 – 374Combined sources8
Beta strandi383 – 387Combined sources5
Turni391 – 393Combined sources3
Helixi394 – 414Combined sources21
Turni421 – 423Combined sources3
Helixi424 – 438Combined sources15
Helixi444 – 455Combined sources12
Helixi457 – 464Combined sources8
Turni465 – 467Combined sources3
Helixi470 – 484Combined sources15
Helixi503 – 505Combined sources3
Helixi510 – 528Combined sources19

3D structure databases

ProteinModelPortaliP48643
SMRiP48643
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TCP-1 chaperonin family.Curated

Phylogenomic databases

eggNOGiKOG0357 Eukaryota
COG0459 LUCA
GeneTreeiENSGT00550000074988
HOGENOMiHOG000226735
HOVERGENiHBG106507
InParanoidiP48643
KOiK09497
OMAiYATDEYG
OrthoDBiEOG091G05G4
PhylomeDBiP48643
TreeFamiTF105638

Family and domain databases

CDDicd03339 TCP1_epsilon, 1 hit
Gene3Di1.10.560.10, 2 hits
3.30.260.10, 2 hits
3.50.7.10, 1 hit
InterProiView protein in InterPro
IPR012718 Chap_CCT_epsi
IPR017998 Chaperone_TCP-1
IPR002194 Chaperonin_TCP-1_CS
IPR002423 Cpn60/TCP-1
IPR027409 GroEL-like_apical_dom_sf
IPR027413 GROEL-like_equatorial_sf
IPR027410 TCP-1-like_intermed_sf
PANTHERiPTHR11353:SF178 PTHR11353:SF178, 1 hit
PfamiView protein in Pfam
PF00118 Cpn60_TCP1, 1 hit
PRINTSiPR00304 TCOMPLEXTCP1
SUPFAMiSSF48592 SSF48592, 2 hits
SSF52029 SSF52029, 1 hit
SSF54849 SSF54849, 2 hits
TIGRFAMsiTIGR02343 chap_CCT_epsi, 1 hit
PROSITEiView protein in PROSITE
PS00750 TCP1_1, 1 hit
PS00751 TCP1_2, 1 hit
PS00995 TCP1_3, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P48643-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASMGTLAFD EYGRPFLIIK DQDRKSRLMG LEALKSHIMA AKAVANTMRT
60 70 80 90 100
SLGPNGLDKM MVDKDGDVTV TNDGATILSM MDVDHQIAKL MVELSKSQDD
110 120 130 140 150
EIGDGTTGVV VLAGALLEEA EQLLDRGIHP IRIADGYEQA ARVAIEHLDK
160 170 180 190 200
ISDSVLVDIK DTEPLIQTAK TTLGSKVVNS CHRQMAEIAV NAVLTVADME
210 220 230 240 250
RRDVDFELIK VEGKVGGRLE DTKLIKGVIV DKDFSHPQMP KKVEDAKIAI
260 270 280 290 300
LTCPFEPPKP KTKHKLDVTS VEDYKALQKY EKEKFEEMIQ QIKETGANLA
310 320 330 340 350
ICQWGFDDEA NHLLLQNNLP AVRWVGGPEI ELIAIATGGR IVPRFSELTA
360 370 380 390 400
EKLGFAGLVQ EISFGTTKDK MLVIEQCKNS RAVTIFIRGG NKMIIEEAKR
410 420 430 440 450
SLHDALCVIR NLIRDNRVVY GGGAAEISCA LAVSQEADKC PTLEQYAMRA
460 470 480 490 500
FADALEVIPM ALSENSGMNP IQTMTEVRAR QVKEMNPALG IDCLHKGTND
510 520 530 540
MKQQHVIETL IGKKQQISLA TQMVRMILKI DDIRKPGESE E
Length:541
Mass (Da):59,671
Last modified:February 1, 1996 - v1
Checksum:i164168BB80EF022A
GO
Isoform 2 (identifier: P48643-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-38: Missing.
     56-110: Missing.

Note: No experimental confirmation available.
Show »
Length:448
Mass (Da):49,526
Checksum:i9B4A960C92BF4327
GO

Sequence cautioni

The sequence BAA07894 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti55N → D in BAF83082 (PubMed:14702039).Curated1
Sequence conflicti512G → D in BAF83082 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052267146E → V. Corresponds to variant dbSNP:rs11557652EnsemblClinVar.1
Natural variantiVAR_030658147H → R in HSNSP. 1 PublicationCorresponds to variant dbSNP:rs118203986EnsemblClinVar.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0540051 – 38Missing in isoform 2. 1 PublicationAdd BLAST38
Alternative sequenceiVSP_05400656 – 110Missing in isoform 2. 1 PublicationAdd BLAST55

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D43950 mRNA Translation: BAA07894.2 Different initiation.
AK289353 mRNA Translation: BAF82042.1
AK290393 mRNA Translation: BAF83082.1
AK302383 mRNA Translation: BAG63700.1
AC012640 Genomic DNA No translation available.
CH471102 Genomic DNA Translation: EAX08072.1
BC006543 mRNA Translation: AAH06543.1
BC035499 mRNA Translation: AAH35499.1
CCDSiCCDS3877.1 [P48643-1]
CCDS82990.1 [P48643-2]
RefSeqiNP_001293084.1, NM_001306155.1 [P48643-2]
NP_036205.1, NM_012073.4 [P48643-1]
UniGeneiHs.1600
Hs.740762

Genome annotation databases

EnsembliENST00000280326; ENSP00000280326; ENSG00000150753 [P48643-1]
ENST00000506600; ENSP00000423052; ENSG00000150753 [P48643-2]
GeneIDi22948
KEGGihsa:22948
UCSCiuc011cmt.3 human [P48643-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiTCPE_HUMAN
AccessioniPrimary (citable) accession number: P48643
Secondary accession number(s): A8JZY8, A8K2X8, B4DYD8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: July 18, 2018
This is version 186 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

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