UniProtKB - P48616 (VIME_BOVIN)
Vimentin
VIM
Functioni
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
By similaritySites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 351 | StutterBy similarity | 1 |
GO - Molecular functioni
- double-stranded RNA binding Source: Ensembl
- identical protein binding Source: Ensembl
- keratin filament binding Source: Ensembl
- protein domain specific binding Source: Ensembl
- scaffold protein binding Source: Ensembl
- structural constituent of cytoskeleton Source: UniProtKB
- structural constituent of eye lens Source: Ensembl
GO - Biological processi
- astrocyte development Source: Ensembl
- Bergmann glial cell differentiation Source: Ensembl
- cellular response to interferon-gamma Source: Ensembl
- cellular response to lipopolysaccharide Source: UniProtKB
- cellular response to muramyl dipeptide Source: UniProtKB
- intermediate filament organization Source: UniProtKB
- in utero embryonic development Source: AgBase
- lens fiber cell development Source: Ensembl
- negative regulation of neuron projection development Source: Ensembl
- positive regulation of collagen biosynthetic process Source: Ensembl
- positive regulation of superoxide anion generation Source: ARUK-UCL
- positive regulation of translation Source: Ensembl
- regulation of mRNA stability Source: Ensembl
- SMAD protein signal transduction Source: Ensembl
Enzyme and pathway databases
Reactomei | R-BTA-264870, Caspase-mediated cleavage of cytoskeletal proteins R-BTA-390522, Striated Muscle Contraction |
Names & Taxonomyi
Protein namesi | Recommended name: Vimentin |
Gene namesi | Name:VIM |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | HostDB:ENSBTAG00000018463 |
VGNCi | VGNC:36796, VIM |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Plasma membrane
- Cell membrane By similarity
Cytoskeleton
- cytoskeleton By similarity
Nucleus
- Nucleus matrix By similarity
Cytoskeleton
- intermediate filament Source: UniProtKB
Cytosol
- cytosol Source: Ensembl
Nucleus
- nuclear matrix Source: UniProtKB-SubCell
- nucleus Source: AgBase
Peroxisome
- peroxisome Source: Ensembl
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- cell leading edge Source: Ensembl
- cytoplasm Source: UniProtKB
- neuron projection Source: Ensembl
- phagocytic vesicle Source: Ensembl
- polysome Source: Ensembl
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Cytoskeleton, Intermediate filament, Membrane, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000063751 | 2 – 466 | VimentinAdd BLAST | 465 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Modified residuei | 5 | PhosphoserineBy similarity | 1 | |
Modified residuei | 7 | Phosphoserine; by PKA and PKC; alternateBy similarity | 1 | |
Glycosylationi | 7 | O-linked (GlcNAc) serine; alternateBy similarity | 1 | |
Modified residuei | 8 | PhosphoserineBy similarity | 1 | |
Modified residuei | 9 | Phosphoserine; by PKCBy similarity | 1 | |
Modified residuei | 10 | Phosphoserine; by PKCBy similarity | 1 | |
Modified residuei | 20 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 25 | Phosphoserine; by PKA and PKCBy similarity | 1 | |
Modified residuei | 26 | Phosphoserine; by PKCBy similarity | 1 | |
Glycosylationi | 33 | O-linked (GlcNAc) threonineBy similarity | 1 | |
Modified residuei | 34 | Phosphoserine; by PKC; alternateBy similarity | 1 | |
Glycosylationi | 34 | O-linked (GlcNAc) serine; alternateBy similarity | 1 | |
Modified residuei | 39 | Phosphoserine; by CaMK2, PKA, PKC and ROCK2By similarity | 1 | |
Modified residuei | 42 | Phosphoserine; by PKCBy similarity | 1 | |
Modified residuei | 49 | PhosphoserineBy similarity | 1 | |
Modified residuei | 53 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 55 | PhosphoserineBy similarity | 1 | |
Modified residuei | 56 | Phosphoserine; by CDK5 and CDK1By similarity | 1 | |
Modified residuei | 61 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 66 | Phosphoserine; by PKA and PKCBy similarity | 1 | |
Modified residuei | 72 | Phosphoserine; by AURKB and ROCK2By similarity | 1 | |
Modified residuei | 83 | Phosphoserine; by CaMK2By similarity | 1 | |
Modified residuei | 87 | PhosphoserineBy similarity | 1 | |
Cross-linki | 104 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 117 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 120 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 120 | N6-succinyllysine; alternateBy similarity | 1 | |
Cross-linki | 120 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 129 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 129 | N6-succinyllysine; alternateBy similarity | 1 | |
Cross-linki | 129 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 139 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 139 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 144 | PhosphoserineBy similarity | 1 | |
Modified residuei | 168 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 188 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 188 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 214 | PhosphoserineBy similarity | 1 | |
Modified residuei | 223 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 223 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 226 | PhosphoserineBy similarity | 1 | |
Modified residuei | 235 | N6-acetyllysineBy similarity | 1 | |
Cross-linki | 262 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 294 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 294 | N6-succinyllysine; alternateBy similarity | 1 | |
Cross-linki | 294 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 299 | PhosphoserineBy similarity | 1 | |
Cross-linki | 313 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 373 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 373 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 409 | PhosphoserineBy similarity | 1 | |
Modified residuei | 412 | PhosphoserineBy similarity | 1 | |
Modified residuei | 419 | PhosphoserineBy similarity | 1 | |
Modified residuei | 420 | PhosphoserineBy similarity | 1 | |
Modified residuei | 426 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 430 | PhosphoserineBy similarity | 1 | |
Modified residuei | 436 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 438 | PhosphoserineBy similarity | 1 | |
Cross-linki | 439 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 445 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 445 | N6-succinyllysine; alternateBy similarity | 1 | |
Cross-linki | 445 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity | ||
Cross-linki | 445 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 446 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 458 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 459 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Glycoprotein, Isopeptide bond, Phosphoprotein, S-nitrosylation, Ubl conjugationProteomic databases
PaxDbi | P48616 |
PeptideAtlasi | P48616 |
PRIDEi | P48616 |
PTM databases
iPTMneti | P48616 |
Expressioni
Gene expression databases
Bgeei | ENSBTAG00000018463, Expressed in subcutaneous adipose tissue and 96 other tissues |
Interactioni
Subunit structurei
Homomer assembled from elementary dimers (By similarity). Identified in complexes that contain VIM, EZR, AHNAK, BFSP1, BFSP2, ANK2, PLEC, PRX and spectrin (By similarity).
Interacts with BCAS3 (By similarity).
Interacts with LGSN (By similarity).
Interacts with SYNM (By similarity).
Interacts (via rod region) with PLEC (via CH 1 domain) (By similarity).
Interacts with STK33 (By similarity).
Interacts with LARP6 (By similarity).
Interacts with RAB8B (By similarity).
Interacts with TOR1A; the interaction associates TOR1A with the cytoskeleton.
Interacts with TOR1AIP1 (By similarity).
Interacts with TOR1AIP1 (By similarity).
Interacts with DIAPH1 (By similarity).
Interacts with EPPK1; interaction is dependent of higher-order structure of intermediate filament (By similarity).
Interacts with the non-receptor tyrosine kinase SRMS; the interaction leads to phosphorylation of VIM (By similarity).
Interacts with NOD2 (By similarity).
Interacts (via head region) with CORO1C (By similarity).
Interacts with HDGF (By similarity).
Interacts with PRKCE (via phorbol-ester/DAG-type 2 domain) (By similarity).
Interacts with BFSP2 (By similarity).
Interacts with PPL (By similarity).
By similarityBinary interactionsi
P48616
With | #Exp. | IntAct |
---|---|---|
TGFB1 [P18341] | 2 | EBI-1221453,EBI-8537762 |
GO - Molecular functioni
- identical protein binding Source: Ensembl
- protein domain specific binding Source: Ensembl
- scaffold protein binding Source: Ensembl
Protein-protein interaction databases
IntActi | P48616, 3 interactors |
MINTi | P48616 |
STRINGi | 9913.ENSBTAP00000024572 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 103 – 411 | IF rodPROSITE-ProRule annotationAdd BLAST | 309 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 31 | DisorderedSequence analysisAdd BLAST | 31 | |
Regioni | 2 – 95 | HeadAdd BLAST | 94 | |
Regioni | 96 – 131 | Coil 1AAdd BLAST | 36 | |
Regioni | 132 – 153 | Linker 1Add BLAST | 22 | |
Regioni | 154 – 245 | Coil 1BAdd BLAST | 92 | |
Regioni | 246 – 268 | Linker 12Add BLAST | 23 | |
Regioni | 269 – 407 | Coil 2Add BLAST | 139 | |
Regioni | 408 – 466 | TailAdd BLAST | 59 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 96 – 131 | Add BLAST | 36 | |
Coiled coili | 154 – 245 | Add BLAST | 92 | |
Coiled coili | 303 – 407 | Add BLAST | 105 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 326 – 329 | [IL]-x-C-x-x-[DE] motifBy similarity | 4 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG0977, Eukaryota |
GeneTreei | ENSGT00940000156146 |
HOGENOMi | CLU_012560_7_4_1 |
InParanoidi | P48616 |
OMAi | PPMRLHD |
OrthoDBi | 655109at2759 |
TreeFami | TF330122 |
Family and domain databases
InterProi | View protein in InterPro IPR018039, IF_conserved IPR039008, IF_rod_dom IPR006821, Intermed_filament_DNA-bd IPR027699, Vimentin |
PANTHERi | PTHR45652:SF5, PTHR45652:SF5, 1 hit |
Pfami | View protein in Pfam PF00038, Filament, 1 hit PF04732, Filament_head, 1 hit |
SMARTi | View protein in SMART SM01391, Filament, 1 hit |
PROSITEi | View protein in PROSITE PS00226, IF_ROD_1, 1 hit PS51842, IF_ROD_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MSTRSVSSSS YRRMFGGPGT ASRPSSTRSY VTTSTRTYSL GSALRPTTSR
60 70 80 90 100
TLYTSSPGGV YATRSSAVRL RSGVPGVRLL QDSVDFSLAD AINTEFKNTR
110 120 130 140 150
TNEKVELQEL NDRFANYIDK VRFLEQQNKI LLAELEQLKG QGKSRLGDLY
160 170 180 190 200
EEEMRELRRQ VDQLTNDKAR VEVERDNLAE DIMRLREKLQ EEMLQREEAE
210 220 230 240 250
STLQSFRQDV DNASLARLDL ERKVESLQEE IAFLKKLHDE EIQELQAQIQ
260 270 280 290 300
EQHVQIDMDV SKPDLTAALR DVRQQYESVA AKNLQEAEEW YKSKFADLSE
310 320 330 340 350
AANRNNDALR QAKQESNEYR RQVQTLTCEV DALKGTNESL ERQMREMEEN
360 370 380 390 400
FSVEAANYQD TIGRLQDEIQ NMKEEMARHL REYQDLLNVK MALDIEIATY
410 420 430 440 450
RKLLEGEESR ISLPLPNFSS LNLRETNLDS LPLVDTHSKR TLLIKTVETR
460
DGQVINETSQ HHDDLE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 148 | D → Q in AAA53661 (PubMed:8144034).Curated | 1 | |
Sequence conflicti | 171 | V → A in AAA53661 (PubMed:8144034).Curated | 1 | |
Sequence conflicti | 176 | D → H in AAA53661 (PubMed:8144034).Curated | 1 | |
Sequence conflicti | 240 | E → A in AAA53661 (PubMed:8144034).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L13263 mRNA Translation: AAA53661.1 BC118269 mRNA Translation: AAI18270.1 |
RefSeqi | NP_776394.2, NM_173969.3 |
Genome annotation databases
Ensembli | ENSBTAT00000024572; ENSBTAP00000024572; ENSBTAG00000018463 |
GeneIDi | 280955 |
KEGGi | bta:280955 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L13263 mRNA Translation: AAA53661.1 BC118269 mRNA Translation: AAI18270.1 |
RefSeqi | NP_776394.2, NM_173969.3 |
3D structure databases
AlphaFoldDBi | P48616 |
SMRi | P48616 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P48616, 3 interactors |
MINTi | P48616 |
STRINGi | 9913.ENSBTAP00000024572 |
PTM databases
iPTMneti | P48616 |
Proteomic databases
PaxDbi | P48616 |
PeptideAtlasi | P48616 |
PRIDEi | P48616 |
Genome annotation databases
Ensembli | ENSBTAT00000024572; ENSBTAP00000024572; ENSBTAG00000018463 |
GeneIDi | 280955 |
KEGGi | bta:280955 |
Organism-specific databases
CTDi | 7431 |
VEuPathDBi | HostDB:ENSBTAG00000018463 |
VGNCi | VGNC:36796, VIM |
Phylogenomic databases
eggNOGi | KOG0977, Eukaryota |
GeneTreei | ENSGT00940000156146 |
HOGENOMi | CLU_012560_7_4_1 |
InParanoidi | P48616 |
OMAi | PPMRLHD |
OrthoDBi | 655109at2759 |
TreeFami | TF330122 |
Enzyme and pathway databases
Reactomei | R-BTA-264870, Caspase-mediated cleavage of cytoskeletal proteins R-BTA-390522, Striated Muscle Contraction |
Gene expression databases
Bgeei | ENSBTAG00000018463, Expressed in subcutaneous adipose tissue and 96 other tissues |
Family and domain databases
InterProi | View protein in InterPro IPR018039, IF_conserved IPR039008, IF_rod_dom IPR006821, Intermed_filament_DNA-bd IPR027699, Vimentin |
PANTHERi | PTHR45652:SF5, PTHR45652:SF5, 1 hit |
Pfami | View protein in Pfam PF00038, Filament, 1 hit PF04732, Filament_head, 1 hit |
SMARTi | View protein in SMART SM01391, Filament, 1 hit |
PROSITEi | View protein in PROSITE PS00226, IF_ROD_1, 1 hit PS51842, IF_ROD_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | VIME_BOVIN | |
Accessioni | P48616Primary (citable) accession number: P48616 Secondary accession number(s): Q17QM7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1996 |
Last sequence update: | May 1, 2007 | |
Last modified: | May 25, 2022 | |
This is version 160 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families