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Entry version 176 (07 Oct 2020)
Sequence version 1 (01 Feb 1996)
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Protein

Homocitrate synthase, cytosolic isozyme

Gene

LYS20

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the aldol-type condensation of 2-oxoglutarate with acetyl-CoA to yield homocitrate. Carries out the first step of the alpha-aminoadipate (AAA) lysine biosynthesis pathway.By similarity

Miscellaneous

Present with 28100 molecules/cell in log phase SD medium.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mg2+By similarity, Mn2+By similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: L-lysine biosynthesis via AAA pathway

This protein is involved in step 1 of the subpathway that synthesizes L-alpha-aminoadipate from 2-oxoglutarate.By similarity
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Homocitrate synthase, cytosolic isozyme (LYS20), Homocitrate synthase, mitochondrial (LYS21)
  2. Homocitrate dehydratase, mitochondrial (ACO2)
  3. Homoaconitase, mitochondrial (LYS4)
  4. Homoisocitrate dehydrogenase, mitochondrial (LYS12)
  5. Aromatic/aminoadipate aminotransferase 1 (ARO8)
This subpathway is part of the pathway L-lysine biosynthesis via AAA pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-alpha-aminoadipate from 2-oxoglutarate, the pathway L-lysine biosynthesis via AAA pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei312-oxoglutarateBy similarity1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi32MagnesiumBy similarity1
Binding sitei912-oxoglutarateBy similarity1
Binding sitei1512-oxoglutarateBy similarity1
Binding sitei1852-oxoglutarateBy similarity1
Metal bindingi212Magnesium; via tele nitrogenBy similarity1
Metal bindingi214Magnesium; via tele nitrogenBy similarity1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei309Proton acceptorBy similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionTransferase
Biological processAmino-acid biosynthesis, Lysine biosynthesis
LigandMagnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
MetaCyc:YDL182W-MONOMER

BRENDA Comprehensive Enzyme Information System

More...
BRENDAi
2.3.3.14, 984

SABIO-RK: Biochemical Reaction Kinetics Database

More...
SABIO-RKi
P48570

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...
UniPathwayi
UPA00033;UER00028

Protein family/group databases

MoonProt database of moonlighting proteins

More...
MoonProti
P48570

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Homocitrate synthase, cytosolic isozyme (EC:2.3.3.14By similarity)
Short name:
HCS
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:LYS20
Ordered Locus Names:YDL182W
ORF Names:D1298
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome IV

Organism-specific databases

Eukaryotic Pathogen Database Resources

More...
EuPathDBi
FungiDB:YDL182W

Saccharomyces Genome Database

More...
SGDi
S000002341, LYS20

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001404541 – 428Homocitrate synthase, cytosolic isozymeAdd BLAST428

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei385PhosphoserineCombined sources1
Modified residuei396PhosphothreonineCombined sources1
Modified residuei401PhosphoserineCombined sources1
Modified residuei410PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase

More...
MaxQBi
P48570

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P48570

PRoteomics IDEntifications database

More...
PRIDEi
P48570

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
P48570

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
31862, 142 interactors

Protein interaction database and analysis system

More...
IntActi
P48570, 5 interactors

Molecular INTeraction database

More...
MINTi
P48570

STRING: functional protein association networks

More...
STRINGi
4932.YDL182W

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...
RNActi
P48570, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P48570

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini23 – 276Pyruvate carboxyltransferasePROSITE-ProRule annotationAdd BLAST254

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG2367, Eukaryota

Ensembl GeneTree

More...
GeneTreei
ENSGT00940000176819

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_022158_2_2_1

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P48570

KEGG Orthology (KO)

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KOi
K01655

Identification of Orthologs from Complete Genome Data

More...
OMAi
SNMFAHE

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.20.20.70, 1 hit

HAMAP database of protein families

More...
HAMAPi
MF_02222, Homocitr_synth_fung_arch, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR002034, AIPM/Hcit_synth_CS
IPR013785, Aldolase_TIM
IPR011872, Homocitrate_synth_fun/arc
IPR000891, PYR_CT

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00682, HMGL-like, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR02146, LysS_fung_arch, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00815, AIPM_HOMOCIT_SYNTH_1, 1 hit
PS00816, AIPM_HOMOCIT_SYNTH_2, 1 hit
PS50991, PYR_CT, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P48570-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTAAKPNPYA AKPGDYLSNV NNFQLIDSTL REGEQFANAF FDTEKKIEIA
60 70 80 90 100
RALDDFGVDY IELTSPVASE QSRKDCEAIC KLGLKAKILT HIRCHMDDAK
110 120 130 140 150
VAVETGVDGV DVVIGTSKFL RQYSHGKDMN YIAKSAVEVI EFVKSKGIEI
160 170 180 190 200
RFSSEDSFRS DLVDLLNIYK TVDKIGVNRV GIADTVGCAN PRQVYELIRT
210 220 230 240 250
LKSVVSCDIE CHFHNDTGCA IANAYTALEG GARLIDVSVL GIGERNGITP
260 270 280 290 300
LGGLMARMIV AAPDYVKSKY KLHKIRDIEN LVADAVEVNI PFNNPITGFC
310 320 330 340 350
AFTHKAGIHA KAILANPSTY EILDPHDFGM KRYIHFANRL TGWNAIKARV
360 370 380 390 400
DQLNLNLTDD QIKEVTAKIK KLGDVRSLNI DDVDSIIKNF HAEVSTPQVL
410 420
SAKKNKKNDS DVPELATIPA AKRTKPSA
Length:428
Mass (Da):47,099
Last modified:February 1, 1996 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i8774FF56FA678E5E
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
X83276 Genomic DNA Translation: CAA58264.1
Z67750 Genomic DNA Translation: CAA91564.1
Z74230 Genomic DNA Translation: CAA98757.1
Z74229 Genomic DNA Translation: CAA98756.1
BK006938 Genomic DNA Translation: DAA11680.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S58735

NCBI Reference Sequences

More...
RefSeqi
NP_010099.1, NM_001180242.1

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
YDL182W_mRNA; YDL182W; YDL182W

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
851346

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sce:YDL182W

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83276 Genomic DNA Translation: CAA58264.1
Z67750 Genomic DNA Translation: CAA91564.1
Z74230 Genomic DNA Translation: CAA98757.1
Z74229 Genomic DNA Translation: CAA98756.1
BK006938 Genomic DNA Translation: DAA11680.1
PIRiS58735
RefSeqiNP_010099.1, NM_001180242.1

3D structure databases

SMRiP48570
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi31862, 142 interactors
IntActiP48570, 5 interactors
MINTiP48570
STRINGi4932.YDL182W

Protein family/group databases

MoonProtiP48570

PTM databases

iPTMnetiP48570

Proteomic databases

MaxQBiP48570
PaxDbiP48570
PRIDEiP48570

Genome annotation databases

EnsemblFungiiYDL182W_mRNA; YDL182W; YDL182W
GeneIDi851346
KEGGisce:YDL182W

Organism-specific databases

EuPathDBiFungiDB:YDL182W
SGDiS000002341, LYS20

Phylogenomic databases

eggNOGiKOG2367, Eukaryota
GeneTreeiENSGT00940000176819
HOGENOMiCLU_022158_2_2_1
InParanoidiP48570
KOiK01655
OMAiSNMFAHE

Enzyme and pathway databases

UniPathwayiUPA00033;UER00028
BioCyciMetaCyc:YDL182W-MONOMER
BRENDAi2.3.3.14, 984
SABIO-RKiP48570

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P48570
RNActiP48570, protein

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_02222, Homocitr_synth_fung_arch, 1 hit
InterProiView protein in InterPro
IPR002034, AIPM/Hcit_synth_CS
IPR013785, Aldolase_TIM
IPR011872, Homocitrate_synth_fun/arc
IPR000891, PYR_CT
PfamiView protein in Pfam
PF00682, HMGL-like, 1 hit
TIGRFAMsiTIGR02146, LysS_fung_arch, 1 hit
PROSITEiView protein in PROSITE
PS00815, AIPM_HOMOCIT_SYNTH_1, 1 hit
PS00816, AIPM_HOMOCIT_SYNTH_2, 1 hit
PS50991, PYR_CT, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiHOSC_YEAST
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P48570
Secondary accession number(s): D6VRH0
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 7, 2020
This is version 176 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families
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