UniProtKB - P48548 (KCNJ5_RAT)
Protein
G protein-activated inward rectifier potassium channel 4
Gene
Kcnj5
Organism
Rattus norvegicus (Rat)
Status
Functioni
This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium.3 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 179 | Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity | 1 |
GO - Molecular functioni
- G-protein activated inward rectifier potassium channel activity Source: InterPro
- inward rectifier potassium channel activity Source: GO_Central
- voltage-gated potassium channel activity involved in atrial cardiac muscle cell action potential repolarization Source: RGD
GO - Biological processi
- membrane repolarization during atrial cardiac muscle cell action potential Source: RGD
- potassium ion import across plasma membrane Source: RGD
- regulation of heart rate by cardiac conduction Source: RGD
- regulation of ion transmembrane transport Source: GO_Central
Keywordsi
Molecular function | Ion channel, Voltage-gated channel |
Biological process | Ion transport, Potassium transport, Transport |
Ligand | Potassium |
Enzyme and pathway databases
Reactomei | R-RNO-1296041, Activation of G protein gated Potassium channels R-RNO-997272, Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits |
Names & Taxonomyi
Protein namesi | Recommended name: G protein-activated inward rectifier potassium channel 4Short name: GIRK-4 Alternative name(s): Cardiac inward rectifier Short name: CIR Heart KATP channel Inward rectifier K(+) channel Kir3.4 KATP-1 Potassium channel, inwardly rectifying subfamily J member 5 |
Gene namesi | Name:Kcnj5 Synonyms:Girk4 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 61971, Kcnj5 |
Subcellular locationi
Other locations
- Membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Plasma Membrane
- external side of plasma membrane Source: RGD
- plasma membrane Source: GO_Central
- T-tubule Source: RGD
- voltage-gated potassium channel complex Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 86 | CytoplasmicBy similarityAdd BLAST | 86 | |
Transmembranei | 87 – 111 | Helical; Name=M1By similarityAdd BLAST | 25 | |
Topological domaini | 112 – 135 | ExtracellularBy similarityAdd BLAST | 24 | |
Intramembranei | 136 – 147 | Helical; Pore-forming; Name=H5By similarityAdd BLAST | 12 | |
Intramembranei | 148 – 154 | Pore-formingBy similarity | 7 | |
Topological domaini | 155 – 163 | ExtracellularBy similarity | 9 | |
Transmembranei | 164 – 185 | Helical; Name=M2By similarityAdd BLAST | 22 | |
Topological domaini | 186 – 419 | CytoplasmicBy similarityAdd BLAST | 234 |
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000154937 | 1 – 419 | G protein-activated inward rectifier potassium channel 4Add BLAST | 419 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 5 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P48548 |
PTM databases
iPTMneti | P48548 |
PhosphoSitePlusi | P48548 |
Expressioni
Tissue specificityi
Most abundant in heart tissue where it is found predominantly in atria. Also found in brain, kidney, liver, spleen, lung and thymus.2 Publications
Gene expression databases
Bgeei | ENSRNOG00000033796, Expressed in heart and 19 other tissues |
Genevisiblei | P48548, RN |
Interactioni
Subunit structurei
Associates with GIRK1 or GIRK2 to form a G-protein-activated heteromultimer pore-forming unit. The resulting inward current is much larger.
2 PublicationsProtein-protein interaction databases
ComplexPortali | CPX-3276, I(KACh) inward rectifier potassium channel complex |
STRINGi | 10116.ENSRNOP00000048134 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 149 – 154 | Selectivity filterBy similarity | 6 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 402 – 405 | Poly-Glu | 4 |
Sequence similaritiesi
Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ5 subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3827, Eukaryota |
GeneTreei | ENSGT01010000222432 |
HOGENOMi | CLU_022738_11_0_1 |
InParanoidi | P48548 |
OMAi | DSRIFMN |
OrthoDBi | 956263at2759 |
PhylomeDBi | P48548 |
Family and domain databases
Gene3Di | 2.60.40.1400, 1 hit |
InterProi | View protein in InterPro IPR014756, Ig_E-set IPR041647, IRK_C IPR016449, K_chnl_inward-rec_Kir IPR003277, K_chnl_inward-rec_Kir3.4 IPR013518, K_chnl_inward-rec_Kir_cyto IPR040445, Kir_TM |
PANTHERi | PTHR11767, PTHR11767, 1 hit |
Pfami | View protein in Pfam PF01007, IRK, 1 hit PF17655, IRK_C, 1 hit |
PIRSFi | PIRSF005465, GIRK_kir, 1 hit |
PRINTSi | PR01330, KIR34CHANNEL PR01320, KIRCHANNEL |
SUPFAMi | SSF81296, SSF81296, 1 hit |
i Sequence
Sequence statusi: Complete.
P48548-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAGDSRNAMN QDMEIGVTSQ DHKKIPKQAR DYIPIATDRT RLLPEGKKPR
60 70 80 90 100
QRYMEKTGKC NVHHGNVQET YRYLSDLFTT LVDLKWRFNL LVFTMVYTIT
110 120 130 140 150
WLFFGFIWWL IAYVRGDLDH VGDQEWIPCV ENLSGFVSAF LFSIETETTI
160 170 180 190 200
GYGFRVITEK CPEGIILLLV QAILGSIVNA FMVGCMFVKI SQPKKRAETL
210 220 230 240 250
MFSNNAVISM RDEKLCLMFR VGDLRNSHIV EASIRAKLIK SRQTKEGEFI
260 270 280 290 300
PLNQTDINVG FDTGDDRLFL VSPLIISHEI NEKSPFWEMS RAQLEQEEFE
310 320 330 340 350
VVVILEGMVE ATGMTCQARS SYMDTEVLWG HRFTPVLTLE KGFYEVDYNT
360 370 380 390 400
FHDTYETNTP SCCAKELAEM KRNGQLLQSL PSPPLLGGCA EAEKEAEAEH
410
DEEEEPNGLS VSRATRGSM
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 188 | V → I in CAA58567 (PubMed:8047164).Curated | 1 | |
Sequence conflicti | 375 | Q → E in CAA58567 (PubMed:8047164).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X83584 mRNA Translation: CAA58567.1 L35771 mRNA Translation: AAC42048.1 D50135 mRNA Translation: BAA08815.1 BC087022 mRNA Translation: AAH87022.1 |
PIRi | S48077 |
RefSeqi | NP_058993.1, NM_017297.2 |
Genome annotation databases
Ensembli | ENSRNOT00000041038; ENSRNOP00000048134; ENSRNOG00000033796 |
GeneIDi | 29713 |
KEGGi | rno:29713 |
UCSCi | RGD:61971, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X83584 mRNA Translation: CAA58567.1 L35771 mRNA Translation: AAC42048.1 D50135 mRNA Translation: BAA08815.1 BC087022 mRNA Translation: AAH87022.1 |
PIRi | S48077 |
RefSeqi | NP_058993.1, NM_017297.2 |
3D structure databases
SMRi | P48548 |
ModBasei | Search... |
Protein-protein interaction databases
ComplexPortali | CPX-3276, I(KACh) inward rectifier potassium channel complex |
STRINGi | 10116.ENSRNOP00000048134 |
Chemistry databases
ChEMBLi | CHEMBL3714708 |
GuidetoPHARMACOLOGYi | 437 |
PTM databases
iPTMneti | P48548 |
PhosphoSitePlusi | P48548 |
Proteomic databases
PaxDbi | P48548 |
Genome annotation databases
Ensembli | ENSRNOT00000041038; ENSRNOP00000048134; ENSRNOG00000033796 |
GeneIDi | 29713 |
KEGGi | rno:29713 |
UCSCi | RGD:61971, rat |
Organism-specific databases
CTDi | 3762 |
RGDi | 61971, Kcnj5 |
Phylogenomic databases
eggNOGi | KOG3827, Eukaryota |
GeneTreei | ENSGT01010000222432 |
HOGENOMi | CLU_022738_11_0_1 |
InParanoidi | P48548 |
OMAi | DSRIFMN |
OrthoDBi | 956263at2759 |
PhylomeDBi | P48548 |
Enzyme and pathway databases
Reactomei | R-RNO-1296041, Activation of G protein gated Potassium channels R-RNO-997272, Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits |
Miscellaneous databases
PROi | PR:P48548 |
Gene expression databases
Bgeei | ENSRNOG00000033796, Expressed in heart and 19 other tissues |
Genevisiblei | P48548, RN |
Family and domain databases
Gene3Di | 2.60.40.1400, 1 hit |
InterProi | View protein in InterPro IPR014756, Ig_E-set IPR041647, IRK_C IPR016449, K_chnl_inward-rec_Kir IPR003277, K_chnl_inward-rec_Kir3.4 IPR013518, K_chnl_inward-rec_Kir_cyto IPR040445, Kir_TM |
PANTHERi | PTHR11767, PTHR11767, 1 hit |
Pfami | View protein in Pfam PF01007, IRK, 1 hit PF17655, IRK_C, 1 hit |
PIRSFi | PIRSF005465, GIRK_kir, 1 hit |
PRINTSi | PR01330, KIR34CHANNEL PR01320, KIRCHANNEL |
SUPFAMi | SSF81296, SSF81296, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KCNJ5_RAT | |
Accessioni | P48548Primary (citable) accession number: P48548 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1996 |
Last sequence update: | November 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 151 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families