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Protein

L-ascorbate peroxidase, cytosolic

Gene

APX1

Organism
Pisum sativum (Garden pea)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a key role in hydrogen peroxide removal.

Miscellaneous

Binds one cation per subunit; probably K+, but might also be Ca2+.

Catalytic activityi

2 L-ascorbate + H2O2 + 2 H+ = L-ascorbate + L-dehydroascorbate + 2 H2O.

Cofactori

heme bNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei38Transition state stabilizer1
Active sitei42Proton acceptor1
Metal bindingi163Iron (heme axial ligand)PROSITE-ProRule annotation1 Publication1
Metal bindingi164Potassium or calcium1
Metal bindingi180Potassium or calcium1
Metal bindingi182Potassium or calcium1
Metal bindingi185Potassium or calcium; via carbonyl oxygen1
Metal bindingi187Potassium or calcium1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase, Peroxidase
Biological processHydrogen peroxide, Stress response
LigandCalcium, Heme, Iron, Metal-binding, Potassium

Enzyme and pathway databases

SABIO-RKiP48534

Protein family/group databases

PeroxiBasei2462 PsAPx01

Names & Taxonomyi

Protein namesi
Recommended name:
L-ascorbate peroxidase, cytosolic (EC:1.11.1.11)
Short name:
AP
Alternative name(s):
PsAPx01
Gene namesi
Name:APX1
Synonyms:APPX1
OrganismiPisum sativum (Garden pea)
Taxonomic identifieri3888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideae50 kb inversion cladeNPAAA cladeHologaleginaIRL cladeFabeaePisum

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00000555972 – 250L-ascorbate peroxidase, cytosolicAdd BLAST249

Proteomic databases

PRIDEiP48534

Expressioni

Inductioni

By stress.1 Publication

Structurei

Secondary structure

1250
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP48534
SMRiP48534
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP48534

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiView protein in InterPro
IPR010255 Haem_peroxidase
IPR002016 Haem_peroxidase_pln/fun/bac
IPR002207 Peroxidase_I
IPR019794 Peroxidases_AS
IPR019793 Peroxidases_heam-ligand_BS
PfamiView protein in Pfam
PF00141 peroxidase, 1 hit
PRINTSiPR00459 ASPEROXIDASE
PR00458 PEROXIDASE
SUPFAMiSSF48113 SSF48113, 1 hit
PROSITEiView protein in PROSITE
PS00435 PEROXIDASE_1, 1 hit
PS00436 PEROXIDASE_2, 1 hit
PS50873 PEROXIDASE_4, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48534-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGKSYPTVSP DYQKAIEKAK RKLRGFIAEK KCAPLILRLA WHSAGTFDSK
60 70 80 90 100
TKTGGPFGTI KHQAELAHGA NNGLDIAVRL LEPIKEQFPI VSYADFYQLA
110 120 130 140 150
GVVAVEITGG PEVPFHPGRE DKPEPPPEGR LPDATKGSDH LRDVFGKAMG
160 170 180 190 200
LSDQDIVALS GGHTIGAAHK ERSGFEGPWT SNPLIFDNSY FTELLTGEKD
210 220 230 240 250
GLLQLPSDKA LLTDSVFRPL VEKYAADEDV FFADYAEAHL KLSELGFAEA
Length:250
Mass (Da):27,193
Last modified:January 23, 2007 - v2
Checksum:i6F51006D0A13B42C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93051 Genomic DNA Translation: AAA33645.1
X62077 mRNA Translation: CAA43992.1
PIRiA45116

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93051 Genomic DNA Translation: AAA33645.1
X62077 mRNA Translation: CAA43992.1
PIRiA45116

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1APXX-ray2.20A/B/C/D2-250[»]
ProteinModelPortaliP48534
SMRiP48534
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

PeroxiBasei2462 PsAPx01

Proteomic databases

PRIDEiP48534

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKiP48534

Miscellaneous databases

EvolutionaryTraceiP48534

Family and domain databases

InterProiView protein in InterPro
IPR010255 Haem_peroxidase
IPR002016 Haem_peroxidase_pln/fun/bac
IPR002207 Peroxidase_I
IPR019794 Peroxidases_AS
IPR019793 Peroxidases_heam-ligand_BS
PfamiView protein in Pfam
PF00141 peroxidase, 1 hit
PRINTSiPR00459 ASPEROXIDASE
PR00458 PEROXIDASE
SUPFAMiSSF48113 SSF48113, 1 hit
PROSITEiView protein in PROSITE
PS00435 PEROXIDASE_1, 1 hit
PS00436 PEROXIDASE_2, 1 hit
PS50873 PEROXIDASE_4, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAPX1_PEA
AccessioniPrimary (citable) accession number: P48534
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: September 12, 2018
This is version 104 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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