UniProtKB - P48508 (GSH0_RAT)
Protein
Glutamate--cysteine ligase regulatory subunit
Gene
Gclm
Organism
Rattus norvegicus (Rat)
Status
Functioni
: glutathione biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.Proteins known to be involved in the 2 steps of the subpathway in this organism are:
- GCS light chain (Gcln), Glutamate--cysteine ligase catalytic subunit (Gclc), Glutamate--cysteine ligase regulatory subunit (Gclm)
- Glutathione synthetase (Gss)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.
GO - Molecular functioni
- enzyme regulator activity Source: GO_Central
- glutamate-cysteine ligase activity Source: RGD
- glutamate-cysteine ligase catalytic subunit binding Source: RGD
- protein-containing complex binding Source: RGD
GO - Biological processi
- aging Source: RGD
- apoptotic mitochondrial changes Source: RGD
- cellular response to fibroblast growth factor stimulus Source: RGD
- cellular response to follicle-stimulating hormone stimulus Source: RGD
- cellular response to glucose stimulus Source: RGD
- cellular response to hepatocyte growth factor stimulus Source: RGD
- cellular response to leukemia inhibitory factor Source: RGD
- cellular response to thyroxine stimulus Source: RGD
- cysteine metabolic process Source: RGD
- glutamate metabolic process Source: UniProtKB
- glutathione biosynthetic process Source: RGD
- glutathione metabolic process Source: RGD
- hepatic stellate cell activation Source: RGD
- negative regulation of extrinsic apoptotic signaling pathway Source: RGD
- negative regulation of neuron apoptotic process Source: RGD
- positive regulation of glutamate-cysteine ligase activity Source: RGD
- regulation of blood vessel diameter Source: UniProtKB
- regulation of mitochondrial depolarization Source: RGD
- response to activity Source: RGD
- response to drug Source: UniProtKB
- response to human chorionic gonadotropin Source: RGD
- response to nitrosative stress Source: RGD
- response to nutrient Source: RGD
- response to oxidative stress Source: RGD
Keywordsi
Biological process | Glutathione biosynthesis |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-10025 |
BRENDAi | 6.3.2.2, 5301 |
Reactomei | R-RNO-174403, Glutathione synthesis and recycling |
SABIO-RKi | P48508 |
UniPathwayi | UPA00142;UER00209 |
Names & Taxonomyi
Protein namesi | Recommended name: Glutamate--cysteine ligase regulatory subunitAlternative name(s): GCS light chain Gamma-ECS regulatory subunit Gamma-glutamylcysteine synthetase regulatory subunit Glutamate--cysteine ligase modifier subunit |
Gene namesi | Name:Gclm Synonyms:Glclr |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 619871, Gclm |
Subcellular locationi
Other locations
- glutamate-cysteine ligase complex Source: RGD
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000192575 | 1 – 274 | Glutamate--cysteine ligase regulatory subunitAdd BLAST | 274 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 59 | PhosphoserineCombined sources | 1 | |
Modified residuei | 263 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | P48508 |
PaxDbi | P48508 |
PRIDEi | P48508 |
PTM databases
iPTMneti | P48508 |
PhosphoSitePlusi | P48508 |
Expressioni
Tissue specificityi
Most abundant in kidney. Also found in liver and testis.
Gene expression databases
Bgeei | ENSRNOG00000013409, Expressed in kidney and 21 other tissues |
Genevisiblei | P48508, RN |
Interactioni
Subunit structurei
Heterodimer of a catalytic heavy chain and a regulatory light chain.
GO - Molecular functioni
Protein-protein interaction databases
BioGRIDi | 248351, 1 interactor |
CORUMi | P48508 |
STRINGi | 10116.ENSRNOP00000018343 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3023, Eukaryota |
GeneTreei | ENSGT00510000047658 |
HOGENOMi | CLU_055657_1_0_1 |
InParanoidi | P48508 |
OMAi | PYWEELQ |
OrthoDBi | 1516283at2759 |
PhylomeDBi | P48508 |
TreeFami | TF105986 |
Family and domain databases
Gene3Di | 3.20.20.100, 1 hit |
InterProi | View protein in InterPro IPR032963, Gclm IPR023210, NADP_OxRdtase_dom IPR036812, NADP_OxRdtase_dom_sf |
PANTHERi | PTHR13295, PTHR13295, 1 hit |
Pfami | View protein in Pfam PF00248, Aldo_ket_red, 1 hit |
SUPFAMi | SSF51430, SSF51430, 1 hit |
i Sequence
Sequence statusi: Complete.
P48508-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGTDSRAAGA LLARASTLHL QTGNLLNWGR LRKKCPSTHS EELRDCIQKT
60 70 80 90 100
LNEWSSQISP DLVREFPDVL ECTMSHAVEK INPDEREEMK VSAKLFIVGS
110 120 130 140 150
NSSSSTRNAV DMACSVLGVA QLDSVIMASP PIEDGVNLSL EHLQPYWEEL
160 170 180 190 200
ENLVQSKKIV AIGTSDLDKT QLEQLYQWAQ VKPNSNQVNL ASCCVMPPDL
210 220 230 240 250
TAFAKQFDIQ LLTHNDPKEL LSEASFQEAL QESIPDIEAQ EWVPLWLLRY
260 270
SVIVKSRGII KSKGYILQAK RKGS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S65555 mRNA Translation: AAB28225.1 L22191 mRNA Translation: AAA41543.1 BC078867 mRNA Translation: AAH78867.1 |
PIRi | A48019 |
RefSeqi | NP_059001.1, NM_017305.2 |
Genome annotation databases
Ensembli | ENSRNOT00000018343; ENSRNOP00000018343; ENSRNOG00000013409 |
GeneIDi | 29739 |
KEGGi | rno:29739 |
UCSCi | RGD:619871, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S65555 mRNA Translation: AAB28225.1 L22191 mRNA Translation: AAA41543.1 BC078867 mRNA Translation: AAH78867.1 |
PIRi | A48019 |
RefSeqi | NP_059001.1, NM_017305.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 248351, 1 interactor |
CORUMi | P48508 |
STRINGi | 10116.ENSRNOP00000018343 |
PTM databases
iPTMneti | P48508 |
PhosphoSitePlusi | P48508 |
Proteomic databases
jPOSTi | P48508 |
PaxDbi | P48508 |
PRIDEi | P48508 |
Genome annotation databases
Ensembli | ENSRNOT00000018343; ENSRNOP00000018343; ENSRNOG00000013409 |
GeneIDi | 29739 |
KEGGi | rno:29739 |
UCSCi | RGD:619871, rat |
Organism-specific databases
CTDi | 2730 |
RGDi | 619871, Gclm |
Phylogenomic databases
eggNOGi | KOG3023, Eukaryota |
GeneTreei | ENSGT00510000047658 |
HOGENOMi | CLU_055657_1_0_1 |
InParanoidi | P48508 |
OMAi | PYWEELQ |
OrthoDBi | 1516283at2759 |
PhylomeDBi | P48508 |
TreeFami | TF105986 |
Enzyme and pathway databases
UniPathwayi | UPA00142;UER00209 |
BioCyci | MetaCyc:MONOMER-10025 |
BRENDAi | 6.3.2.2, 5301 |
Reactomei | R-RNO-174403, Glutathione synthesis and recycling |
SABIO-RKi | P48508 |
Miscellaneous databases
PROi | PR:P48508 |
Gene expression databases
Bgeei | ENSRNOG00000013409, Expressed in kidney and 21 other tissues |
Genevisiblei | P48508, RN |
Family and domain databases
Gene3Di | 3.20.20.100, 1 hit |
InterProi | View protein in InterPro IPR032963, Gclm IPR023210, NADP_OxRdtase_dom IPR036812, NADP_OxRdtase_dom_sf |
PANTHERi | PTHR13295, PTHR13295, 1 hit |
Pfami | View protein in Pfam PF00248, Aldo_ket_red, 1 hit |
SUPFAMi | SSF51430, SSF51430, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GSH0_RAT | |
Accessioni | P48508Primary (citable) accession number: P48508 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1996 |
Last sequence update: | February 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 136 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families