UniProtKB - P48500 (TPIS_RAT)
Protein
Triosephosphate isomerase
Gene
Tpi1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Triosephosphate isomerase is an extremely efficient metabolic enzyme that catalyzes the interconversion between dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (G3P) in glycolysis and gluconeogenesis.By similarity
It is also responsible for the non-negligible production of methylglyoxal a reactive cytotoxic side-product that modifies and can alter proteins, DNA and lipids.By similarity
Catalytic activityi
: glycolysis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate.PROSITE-ProRule annotationProteins known to be involved in this subpathway in this organism are:
- Triosephosphate isomerase (Tpi1), Triosephosphate isomerase (LOC500959), Triosephosphate isomerase (LOC246267), Triosephosphate isomerase (LOC100911515), Triosephosphate isomerase
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate, the pathway glycolysis and in Carbohydrate degradation.
Pathwayi: gluconeogenesis
This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.PROSITE-ProRule annotationView all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 12 | SubstratePROSITE-ProRule annotation | 1 | |
Binding sitei | 14 | SubstratePROSITE-ProRule annotation | 1 | |
Active sitei | 96 | ElectrophilePROSITE-ProRule annotation | 1 | |
Active sitei | 166 | Proton acceptorPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- isomerase activity Source: RGD
- methylglyoxal synthase activity Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
- triose-phosphate isomerase activity Source: RGD
- ubiquitin protein ligase binding Source: RGD
GO - Biological processi
- carbohydrate metabolic process Source: RGD
- gluconeogenesis Source: GO_Central
- glucose metabolic process Source: RGD
- glyceraldehyde-3-phosphate biosynthetic process Source: UniProtKB
- glyceraldehyde-3-phosphate metabolic process Source: RGD
- glycerol catabolic process Source: GO_Central
- glycolytic process Source: GO_Central
- methylglyoxal biosynthetic process Source: UniProtKB
- multicellular organism development Source: RGD
Keywordsi
Molecular function | Isomerase, Lyase |
Biological process | Gluconeogenesis, Glycolysis |
Enzyme and pathway databases
Reactomei | R-RNO-70171, Glycolysis R-RNO-70263, Gluconeogenesis |
SABIO-RKi | P48500 |
UniPathwayi | UPA00109;UER00189 UPA00138 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Tpi1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3896, Tpi1 |
Subcellular locationi
Other locations
- Cytoplasm PROSITE-ProRule annotation
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000090120 | 2 – 249 | Triosephosphate isomeraseAdd BLAST | 248 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 14 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 68 | 3'-nitrotyrosineBy similarity | 1 | |
Modified residuei | 80 | PhosphoserineBy similarity | 1 | |
Modified residuei | 106 | PhosphoserineCombined sources | 1 | |
Cross-linki | 142 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity | ||
Modified residuei | 149 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 156 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 156 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 173 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 194 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 194 | N6-methyllysine; alternateBy similarity | 1 | |
Modified residuei | 194 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 198 | PhosphoserineCombined sources | 1 | |
Modified residuei | 209 | 3'-nitrotyrosineBy similarity | 1 | |
Modified residuei | 212 | PhosphoserineBy similarity | 1 | |
Modified residuei | 214 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 223 | PhosphoserineBy similarity | 1 | |
Modified residuei | 238 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
Acetylation, Isopeptide bond, Methylation, Nitration, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | P48500 |
PaxDbi | P48500 |
PRIDEi | P48500 |
2D gel databases
World-2DPAGEi | 0004:P48500 |
PTM databases
iPTMneti | P48500 |
PhosphoSitePlusi | P48500 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000015290, Expressed in skeletal muscle tissue and 21 other tissues |
ExpressionAtlasi | P48500, baseline |
Genevisiblei | P48500, RN |
Interactioni
Subunit structurei
Homodimer.
PROSITE-ProRule annotationGO - Molecular functioni
- protein homodimerization activity Source: UniProtKB
- ubiquitin protein ligase binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 246967, 5 interactors |
CORUMi | P48500 |
IntActi | P48500, 2 interactors |
STRINGi | 10116.ENSRNOP00000067409 |
Family & Domainsi
Sequence similaritiesi
Belongs to the triosephosphate isomerase family.Curated
Phylogenomic databases
eggNOGi | KOG1643, Eukaryota |
GeneTreei | ENSGT00390000013354 |
HOGENOMi | CLU_024251_2_0_1 |
InParanoidi | P48500 |
OMAi | IEKNGTM |
OrthoDBi | 1272577at2759 |
PhylomeDBi | P48500 |
TreeFami | TF300829 |
Family and domain databases
CDDi | cd00311, TIM, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_00147_B, TIM_B, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR035990, TIM_sf IPR022896, TrioseP_Isoase_bac/euk IPR000652, Triosephosphate_isomerase IPR020861, Triosephosphate_isomerase_AS |
PANTHERi | PTHR21139, PTHR21139, 1 hit |
Pfami | View protein in Pfam PF00121, TIM, 1 hit |
SUPFAMi | SSF51351, SSF51351, 1 hit |
TIGRFAMsi | TIGR00419, tim, 1 hit |
PROSITEi | View protein in PROSITE PS00171, TIM_1, 1 hit PS51440, TIM_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P48500-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAPSRKFFVG GNWKMNGRKK CLGELICTLN AAKLPADTEV VCAPPTAYID
60 70 80 90 100
FARQKLDPKI AVAAQNCYKV TNGAFTGEIS PGMIKDLGAT WVVLGHSERR
110 120 130 140 150
HIFGESDELI GQKVNHALSE GLGVIACIGE KLDEREAGIT EKVVFEQTKA
160 170 180 190 200
IADNVKDWCK VVLAYEPVWA IGTGKTATPQ QAQEVHEKLR GWLKCNVSEG
210 220 230 240
VAQCTRIIYG GSVTGATCKE LASQPDVDGF LVGGASLKPE FVDIINAKQ
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JWJ4 | A0A0G2JWJ4_RAT | Triosephosphate isomerase | LOC100911515 Tpi1 | 263 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 123 | G → E in AAA42278 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L36250 mRNA Translation: AAA42278.1 BC061781 mRNA Translation: AAH61781.1 BC126087 mRNA Translation: AAI26088.1 |
RefSeqi | NP_075211.2, NM_022922.2 |
Genome annotation databases
Ensembli | ENSRNOT00000020647; ENSRNOP00000020647; ENSRNOG00000015290 ENSRNOT00000071439; ENSRNOP00000067409; ENSRNOG00000050669 |
GeneIDi | 24849 |
KEGGi | rno:24849 |
UCSCi | RGD:3896, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L36250 mRNA Translation: AAA42278.1 BC061781 mRNA Translation: AAH61781.1 BC126087 mRNA Translation: AAI26088.1 |
RefSeqi | NP_075211.2, NM_022922.2 |
3D structure databases
SMRi | P48500 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 246967, 5 interactors |
CORUMi | P48500 |
IntActi | P48500, 2 interactors |
STRINGi | 10116.ENSRNOP00000067409 |
Chemistry databases
ChEMBLi | CHEMBL2176801 |
PTM databases
iPTMneti | P48500 |
PhosphoSitePlusi | P48500 |
2D gel databases
World-2DPAGEi | 0004:P48500 |
Proteomic databases
jPOSTi | P48500 |
PaxDbi | P48500 |
PRIDEi | P48500 |
Genome annotation databases
Ensembli | ENSRNOT00000020647; ENSRNOP00000020647; ENSRNOG00000015290 ENSRNOT00000071439; ENSRNOP00000067409; ENSRNOG00000050669 |
GeneIDi | 24849 |
KEGGi | rno:24849 |
UCSCi | RGD:3896, rat |
Organism-specific databases
CTDi | 7167 |
RGDi | 3896, Tpi1 |
Phylogenomic databases
eggNOGi | KOG1643, Eukaryota |
GeneTreei | ENSGT00390000013354 |
HOGENOMi | CLU_024251_2_0_1 |
InParanoidi | P48500 |
OMAi | IEKNGTM |
OrthoDBi | 1272577at2759 |
PhylomeDBi | P48500 |
TreeFami | TF300829 |
Enzyme and pathway databases
UniPathwayi | UPA00109;UER00189 UPA00138 |
Reactomei | R-RNO-70171, Glycolysis R-RNO-70263, Gluconeogenesis |
SABIO-RKi | P48500 |
Miscellaneous databases
PROi | PR:P48500 |
Gene expression databases
Bgeei | ENSRNOG00000015290, Expressed in skeletal muscle tissue and 21 other tissues |
ExpressionAtlasi | P48500, baseline |
Genevisiblei | P48500, RN |
Family and domain databases
CDDi | cd00311, TIM, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_00147_B, TIM_B, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR035990, TIM_sf IPR022896, TrioseP_Isoase_bac/euk IPR000652, Triosephosphate_isomerase IPR020861, Triosephosphate_isomerase_AS |
PANTHERi | PTHR21139, PTHR21139, 1 hit |
Pfami | View protein in Pfam PF00121, TIM, 1 hit |
SUPFAMi | SSF51351, SSF51351, 1 hit |
TIGRFAMsi | TIGR00419, tim, 1 hit |
PROSITEi | View protein in PROSITE PS00171, TIM_1, 1 hit PS51440, TIM_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TPIS_RAT | |
Accessioni | P48500Primary (citable) accession number: P48500 Secondary accession number(s): A0JN18, Q6P793 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1996 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 157 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families