UniProtKB - P48462 (PP1B_DROME)
Protein
Serine/threonine-protein phosphatase beta isoform
Gene
flw
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Required for cell adhesion in non-muscle tissues and in maintenance of muscle attachment. Vital for larval development.1 Publication
Catalytic activityi
Cofactori
Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 63 | Manganese 1By similarity | 1 | |
Metal bindingi | 65 | Manganese 1By similarity | 1 | |
Metal bindingi | 91 | Manganese 1By similarity | 1 | |
Metal bindingi | 91 | Manganese 2By similarity | 1 | |
Metal bindingi | 123 | Manganese 2By similarity | 1 | |
Active sitei | 124 | Proton donorBy similarity | 1 | |
Metal bindingi | 172 | Manganese 2By similarity | 1 | |
Metal bindingi | 247 | Manganese 2By similarity | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- myosin phosphatase activity Source: FlyBase
- protein serine/threonine phosphatase activity Source: GO_Central
- protein serine phosphatase activity Source: UniProtKB-EC
- protein threonine phosphatase activity Source: UniProtKB-EC
GO - Biological processi
- beta-catenin destruction complex disassembly Source: FlyBase
- cell adhesion Source: UniProtKB-KW
- chromosome segregation Source: FlyBase
- circadian rhythm Source: Reactome
- female germline ring canal formation Source: FlyBase
- imaginal disc-derived wing morphogenesis Source: FlyBase
- mesoderm development Source: FlyBase
- negative regulation of JNK cascade Source: FlyBase
- negative regulation of smoothened signaling pathway Source: FlyBase
- oocyte nucleus migration involved in oocyte dorsal/ventral axis specification Source: FlyBase
- ovarian nurse cell to oocyte transport Source: FlyBase
- positive regulation of canonical Wnt signaling pathway Source: FlyBase
- regulation of actomyosin contractile ring contraction Source: FlyBase
- striated muscle tissue development Source: FlyBase
Keywordsi
Molecular function | Hydrolase, Protein phosphatase |
Biological process | Cell adhesion |
Ligand | Manganese, Metal-binding |
Enzyme and pathway databases
Reactomei | R-DME-2565942, Regulation of PLK1 Activity at G2/M Transition R-DME-350416, Regulation of non-muscle Myosin II R-DME-350480, Activation of non-muscle Myosin II R-DME-538898, Dephosphorylation of TIM R-DME-5625740, RHO GTPases activate PKNs R-DME-5627123, RHO GTPases activate PAKs |
SignaLinki | P48462 |
Names & Taxonomyi
Protein namesi | Recommended name: Serine/threonine-protein phosphatase beta isoform (EC:3.1.3.161 Publication)Alternative name(s): Protein flap wing |
Gene namesi | Name:flw Synonyms:PP1-9C, PP1-BETA-9C ORF Names:CG2096 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0000711, flw |
Subcellular locationi
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 133 | Y → F: Semi lethal, adult escapers exhibit muscle and wing mutant phenotype; allele flw-6. 1 Publication | 1 | |
Mutagenesisi | 284 | V → A: Muscle and wing mutant phenotype; allele flw-1. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000058794 | 1 – 330 | Serine/threonine-protein phosphatase beta isoformAdd BLAST | 330 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 315 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 316 | Phosphothreonine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P48462 |
PRIDEi | P48462 |
PTM databases
iPTMneti | P48462 |
Expressioni
Gene expression databases
Bgeei | FBgn0000711, Expressed in head and 42 other tissues |
ExpressionAtlasi | P48462, baseline and differential |
Genevisiblei | P48462, DM |
Interactioni
Subunit structurei
Binary interactionsi
P48462
With | #Exp. | IntAct |
---|---|---|
Nop17l [Q0E9G3] | 3 | EBI-869621,EBI-150380 |
Protein-protein interaction databases
BioGRIDi | 68952, 130 interactors |
IntActi | P48462, 28 interactors |
STRINGi | 7227.FBpp0071381 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0374, Eukaryota |
GeneTreei | ENSGT00940000154644 |
HOGENOMi | CLU_004962_0_0_1 |
InParanoidi | P48462 |
OMAi | NVRIWKT |
OrthoDBi | 766640at2759 |
PhylomeDBi | P48462 |
Family and domain databases
Gene3Di | 3.60.21.10, 1 hit |
InterProi | View protein in InterPro IPR004843, Calcineurin-like_PHP_ApaH IPR029052, Metallo-depent_PP-like IPR006186, Ser/Thr-sp_prot-phosphatase IPR031675, STPPase_N |
Pfami | View protein in Pfam PF00149, Metallophos, 1 hit PF16891, STPPase_N, 1 hit |
PRINTSi | PR00114, STPHPHTASE |
SMARTi | View protein in SMART SM00156, PP2Ac, 1 hit |
PROSITEi | View protein in PROSITE PS00125, SER_THR_PHOSPHATASE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
P48462-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGDFDLNVDS LIQRLLEMRS CRTGKQVQMT EAEVRGLCLK SREIFLQQPI
60 70 80 90 100
LLELEAPLII CGDIHGQYTD LLRLFEYGGF PPAANYLFLG DYVDRGKQSL
110 120 130 140 150
ETICLLLAYK IKYPENFFLL RGNHECASIN RIYGFYDECK RRYNVKLWKT
160 170 180 190 200
FTDCFNCLPV AAIIDEKIFC CHGGLSPDLQ GMEQIRRLMR PTDVPDTGLL
210 220 230 240 250
CDLLWSDPDK DVQGWGENDR GVSFTFGVDV VSKFLNRHEL DLICRAHQVV
260 270 280 290 300
EDGYEFFARR QLVTLFSAPN YCGEFDNAGG MMTVDDTLMC SFQILKPSEK
310 320 330
KAKYLYSGMN SSRPTTPQRS APMLATNKKK
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH5V895 | H5V895_DROME | Serine/threonine-protein phosphatas... | flw anon-WO03040301.120, CG15305, Dmel\CG2096, DmPp1-9C, FLW | 330 | Annotation score: | ||
Q8T0U6 | Q8T0U6_DROME | Serine/threonine-protein phosphatas... | flw anon-WO03040301.120, CG15305, Dmel\CG2096, DmPp1-9C, FLW | 461 | Annotation score: | ||
A0A4D6K293 | A0A4D6K293_DROME | Serine/threonine-protein phosphatas... | flw anon-WO03040301.120, CG15305, Dmel\CG2096, DmPp1-9C, FLW | 339 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X56439 mRNA Translation: CAA39821.1 AJ249214, AJ249215 Genomic DNA Translation: CAB59732.1 AE014298 Genomic DNA Translation: AAF46583.2 |
PIRi | S13828 |
RefSeqi | NP_001259407.1, NM_001272478.1 NP_524738.1, NM_079999.3 |
Genome annotation databases
EnsemblMetazoai | FBtr0071447; FBpp0071382; FBgn0000711 FBtr0333305; FBpp0305497; FBgn0000711 |
GeneIDi | 44289 |
KEGGi | dme:Dmel_CG2096 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X56439 mRNA Translation: CAA39821.1 AJ249214, AJ249215 Genomic DNA Translation: CAB59732.1 AE014298 Genomic DNA Translation: AAF46583.2 |
PIRi | S13828 |
RefSeqi | NP_001259407.1, NM_001272478.1 NP_524738.1, NM_079999.3 |
3D structure databases
SMRi | P48462 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 68952, 130 interactors |
IntActi | P48462, 28 interactors |
STRINGi | 7227.FBpp0071381 |
PTM databases
iPTMneti | P48462 |
Proteomic databases
PaxDbi | P48462 |
PRIDEi | P48462 |
Genome annotation databases
EnsemblMetazoai | FBtr0071447; FBpp0071382; FBgn0000711 FBtr0333305; FBpp0305497; FBgn0000711 |
GeneIDi | 44289 |
KEGGi | dme:Dmel_CG2096 |
Organism-specific databases
CTDi | 44289 |
FlyBasei | FBgn0000711, flw |
Phylogenomic databases
eggNOGi | KOG0374, Eukaryota |
GeneTreei | ENSGT00940000154644 |
HOGENOMi | CLU_004962_0_0_1 |
InParanoidi | P48462 |
OMAi | NVRIWKT |
OrthoDBi | 766640at2759 |
PhylomeDBi | P48462 |
Enzyme and pathway databases
Reactomei | R-DME-2565942, Regulation of PLK1 Activity at G2/M Transition R-DME-350416, Regulation of non-muscle Myosin II R-DME-350480, Activation of non-muscle Myosin II R-DME-538898, Dephosphorylation of TIM R-DME-5625740, RHO GTPases activate PKNs R-DME-5627123, RHO GTPases activate PAKs |
SignaLinki | P48462 |
Miscellaneous databases
BioGRID-ORCSi | 44289, 1 hit in 3 CRISPR screens |
ChiTaRSi | flw, fly |
GenomeRNAii | 44289 |
PROi | PR:P48462 |
Gene expression databases
Bgeei | FBgn0000711, Expressed in head and 42 other tissues |
ExpressionAtlasi | P48462, baseline and differential |
Genevisiblei | P48462, DM |
Family and domain databases
Gene3Di | 3.60.21.10, 1 hit |
InterProi | View protein in InterPro IPR004843, Calcineurin-like_PHP_ApaH IPR029052, Metallo-depent_PP-like IPR006186, Ser/Thr-sp_prot-phosphatase IPR031675, STPPase_N |
Pfami | View protein in Pfam PF00149, Metallophos, 1 hit PF16891, STPPase_N, 1 hit |
PRINTSi | PR00114, STPHPHTASE |
SMARTi | View protein in SMART SM00156, PP2Ac, 1 hit |
PROSITEi | View protein in PROSITE PS00125, SER_THR_PHOSPHATASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PP1B_DROME | |
Accessioni | P48462Primary (citable) accession number: P48462 Secondary accession number(s): Q9W2V5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1996 |
Last sequence update: | February 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 182 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families