UniProtKB - P48454 (PP2BC_HUMAN)
Serine/threonine-protein phosphatase 2B catalytic subunit gamma isoform
PPP3CC
Functioni
Catalytic activityi
Cofactori
Protein has several cofactor binding sites:- Fe3+By similarityNote: Binds 1 Fe3+ ion per subunit.By similarity
- Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity
Activity regulationi
Kineticsi
- KM=1.27 µM for NFATC11 Publication
- KM=0.94 µM for DARPP321 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 86 | IronBy similarity | 1 | |
Metal bindingi | 88 | Iron; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 114 | IronBy similarity | 1 | |
Metal bindingi | 114 | ZincBy similarity | 1 | |
Metal bindingi | 146 | ZincBy similarity | 1 | |
Active sitei | 147 | Proton donorBy similarity | 1 | |
Metal bindingi | 195 | Zinc; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 277 | Zinc; via pros nitrogenBy similarity | 1 |
GO - Molecular functioni
- calmodulin binding Source: GO_Central
- calmodulin-dependent protein phosphatase activity Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- brain development Source: Ensembl
- calcineurin-mediated signaling Source: GO_Central
- calcineurin-NFAT signaling cascade Source: UniProtKB
- positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway Source: Reactome
- positive regulation of synaptic vesicle endocytosis Source: Ensembl
- protein dephosphorylation Source: UniProtKB
Keywordsi
Molecular function | Calmodulin-binding, Hydrolase, Protein phosphatase |
Ligand | Iron, Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | P48454 |
Reactomei | R-HSA-111447, Activation of BAD and translocation to mitochondria R-HSA-180024, DARPP-32 events |
SIGNORi | P48454 |
Names & Taxonomyi
Protein namesi | Recommended name: Serine/threonine-protein phosphatase 2B catalytic subunit gamma isoform (EC:3.1.3.161 Publication)Alternative name(s): CAM-PRP catalytic subunit Calcineurin, testis-specific catalytic subunit Calmodulin-dependent calcineurin A subunit gamma isoform |
Gene namesi | Name:PPP3CC Synonyms:CALNA3, CNA3 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000120910.14 |
HGNCi | HGNC:9316, PPP3CC |
MIMi | 114107, gene |
neXtProti | NX_P48454 |
Subcellular locationi
Cytosol
- cytosol Source: Reactome
- presynaptic cytosol Source: Ensembl
Mitochondrion
- mitochondrion Source: GOC
Other locations
- calcineurin complex Source: GO_Central
- cytoplasm Source: UniProtKB
- glutamatergic synapse Source: Ensembl
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 5533 |
OpenTargetsi | ENSG00000120910 |
PharmGKBi | PA33680 |
Miscellaneous databases
Pharosi | P48454, Tbio |
Chemistry databases
DrugCentrali | P48454 |
Polymorphism and mutation databases
BioMutai | PPP3CC |
DMDMi | 257051041 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000058828 | 1 – 512 | Serine/threonine-protein phosphatase 2B catalytic subunit gamma isoformAdd BLAST | 512 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 483 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | P48454 |
jPOSTi | P48454 |
MassIVEi | P48454 |
MaxQBi | P48454 |
PaxDbi | P48454 |
PeptideAtlasi | P48454 |
PRIDEi | P48454 |
ProteomicsDBi | 4973 55894 [P48454-1] 55895 [P48454-2] |
PTM databases
DEPODi | PPP3CC |
iPTMneti | P48454 |
PhosphoSitePlusi | P48454 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000120910, Expressed in testis and 239 other tissues |
ExpressionAtlasi | P48454, baseline and differential |
Genevisiblei | P48454, HS |
Organism-specific databases
HPAi | ENSG00000120910, Low tissue specificity |
Interactioni
Subunit structurei
Forms a complex composed of a calmodulin-dependent catalytic subunit (also known as calcineurin A) and a regulatory Ca2+-binding subunit (also known as calcineurin B) (PubMed:19154138). There are three catalytic subunits, each encoded by a separate gene (PPP3CA, PPP3CB, and PPP3CC) and two regulatory subunits which are also encoded by separate genes (PPP3R1 and PPP3R2). In response to an increase in Ca2+ intracellular levels, forms a complex composed of PPP3CC/calcineurin A, calcineurin B and calmodulin (By similarity).
Interacts (via calmodulin-binding domain) with calmodulin; the interaction depends on calmodulin binding to Ca2+ (By similarity).
Interacts with UNC119 (By similarity).
By similarity1 PublicationBinary interactionsi
Hide detailsP48454
With | #Exp. | IntAct |
---|---|---|
AMPH [P49418] | 3 | EBI-2827192,EBI-7121510 |
PPP3R1 [P63098] | 4 | EBI-2827192,EBI-915984 |
SPATA33 [Q96N06] | 3 | EBI-2827192,EBI-17572815 |
UNC119 [Q13432] | 3 | EBI-2827192,EBI-711260 |
GO - Molecular functioni
- calmodulin binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 111525, 46 interactors |
ComplexPortali | CPX-1001, Calcineurin-Calmodulin complex, gamma-R1 variant CPX-1050, Calcineurin-Calmodulin complex, gamma-R2 variant CPX-1112, Calcineurin-Calmodulin-AKAP5 complex, gamma-R1 variant CPX-1118, Calcineurin-Calmodulin-AKAP5 complex, gamma-R2 variant |
IntActi | P48454, 35 interactors |
STRINGi | 9606.ENSP00000380878 |
Chemistry databases
BindingDBi | P48454 |
Miscellaneous databases
RNActi | P48454, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 52 – 343 | CatalyticCuratedAdd BLAST | 292 | |
Regioni | 344 – 366 | Calcineurin B bindingBy similarityAdd BLAST | 23 | |
Regioni | 386 – 400 | Calmodulin-bindingBy similarityAdd BLAST | 15 | |
Regioni | 401 – 408 | Autoinhibitory segmentBy similarity | 8 | |
Regioni | 459 – 481 | Autoinhibitory domainBy similarityAdd BLAST | 23 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 303 – 307 | SAPNY motifBy similarity | 5 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0375, Eukaryota |
GeneTreei | ENSGT00940000154115 |
InParanoidi | P48454 |
OMAi | ACMNTFD |
OrthoDBi | 463522at2759 |
PhylomeDBi | P48454 |
TreeFami | TF105557 |
Family and domain databases
CDDi | cd07416, MPP_PP2B, 1 hit |
Gene3Di | 3.60.21.10, 1 hit |
InterProi | View protein in InterPro IPR004843, Calcineurin-like_PHP_ApaH IPR029052, Metallo-depent_PP-like IPR041751, MPP_PP2B IPR043360, PP2B IPR006186, Ser/Thr-sp_prot-phosphatase |
PANTHERi | PTHR45673, PTHR45673, 1 hit |
Pfami | View protein in Pfam PF00149, Metallophos, 1 hit |
PRINTSi | PR00114, STPHPHTASE |
SMARTi | View protein in SMART SM00156, PP2Ac, 1 hit |
PROSITEi | View protein in PROSITE PS00125, SER_THR_PHOSPHATASE, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSGRRFHLST TDRVIKAVPF PPTQRLTFKE VFENGKPKVD VLKNHLVKEG
60 70 80 90 100
RLEEEVALKI INDGAAILRQ EKTMIEVDAP ITVCGDIHGQ FFDLMKLFEV
110 120 130 140 150
GGSPSNTRYL FLGDYVDRGY FSIECVLYLW SLKINHPKTL FLLRGNHECR
160 170 180 190 200
HLTDYFTFKQ ECRIKYSEQV YDACMETFDC LPLAALLNQQ FLCVHGGMSP
210 220 230 240 250
EITSLDDIRK LDRFTEPPAF GPVCDLLWSD PSEDYGNEKT LEHYTHNTVR
260 270 280 290 300
GCSYFYSYPA VCEFLQNNNL LSIIRAHEAQ DAGYRMYRKS QATGFPSLIT
310 320 330 340 350
IFSAPNYLDV YNNKAAVLKY ENNVMNIRQF NCSPHPYWLP NFMDVFTWSL
360 370 380 390 400
PFVGEKVTEM LVNVLNICSD DELISDDEAE GSTTVRKEII RNKIRAIGKM
410 420 430 440 450
ARVFSILRQE SESVLTLKGL TPTGTLPLGV LSGGKQTIET ATVEAVEARE
460 470 480 490 500
AIRGFSLQHK IRSFEEARGL DRINERMPPR KDSIHAGGPM KSVTSAHSHA
510
AHRSDQGKKA HS
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3V111 | G3V111_HUMAN | Serine/threonine-protein phosphatas... | PPP3CC hCG_15020 | 326 | Annotation score: | ||
H0YB04 | H0YB04_HUMAN | Serine/threonine-protein phosphatas... | PPP3CC | 202 | Annotation score: | ||
H0YC26 | H0YC26_HUMAN | Protein-serine/threonine phosphatas... | PPP3CC | 209 | Annotation score: | ||
E5RJH4 | E5RJH4_HUMAN | Protein-serine/threonine phosphatas... | PPP3CC | 134 | Annotation score: | ||
H0YB02 | H0YB02_HUMAN | Protein-serine/threonine phosphatas... | PPP3CC | 153 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 485 – 486 | HA → YP in AAB23769 (PubMed:1339277).Curated | 2 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_061758 | 501 | A → V. Corresponds to variant dbSNP:rs28764007Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_045211 | 380 | E → EDHYIPSYQK in isoform 3. 1 Publication | 1 | |
Alternative sequenceiVSP_037946 | 442 – 451 | Missing in isoform 2. 2 Publications | 10 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S46622 mRNA Translation: AAB23769.1 AY007249 mRNA Translation: AAG02563.1 AK299415 mRNA Translation: BAG61397.1 AC037459 Genomic DNA No translation available. AC087854 Genomic DNA No translation available. CH471080 Genomic DNA Translation: EAW63677.1 CH471080 Genomic DNA Translation: EAW63679.1 BC004864 mRNA Translation: AAH04864.1 |
CCDSi | CCDS34859.1 [P48454-1] CCDS59093.1 [P48454-3] CCDS59094.1 [P48454-2] |
PIRi | JC1283 |
RefSeqi | NP_001230903.1, NM_001243974.1 [P48454-3] NP_001230904.1, NM_001243975.1 [P48454-2] NP_005596.2, NM_005605.4 [P48454-1] |
Genome annotation databases
Ensembli | ENST00000240139; ENSP00000240139; ENSG00000120910 [P48454-1] ENST00000289963; ENSP00000289963; ENSG00000120910 [P48454-2] ENST00000397775; ENSP00000380878; ENSG00000120910 [P48454-3] |
GeneIDi | 5533 |
KEGGi | hsa:5533 |
UCSCi | uc003xbs.4, human [P48454-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S46622 mRNA Translation: AAB23769.1 AY007249 mRNA Translation: AAG02563.1 AK299415 mRNA Translation: BAG61397.1 AC037459 Genomic DNA No translation available. AC087854 Genomic DNA No translation available. CH471080 Genomic DNA Translation: EAW63677.1 CH471080 Genomic DNA Translation: EAW63679.1 BC004864 mRNA Translation: AAH04864.1 |
CCDSi | CCDS34859.1 [P48454-1] CCDS59093.1 [P48454-3] CCDS59094.1 [P48454-2] |
PIRi | JC1283 |
RefSeqi | NP_001230903.1, NM_001243974.1 [P48454-3] NP_001230904.1, NM_001243975.1 [P48454-2] NP_005596.2, NM_005605.4 [P48454-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 111525, 46 interactors |
ComplexPortali | CPX-1001, Calcineurin-Calmodulin complex, gamma-R1 variant CPX-1050, Calcineurin-Calmodulin complex, gamma-R2 variant CPX-1112, Calcineurin-Calmodulin-AKAP5 complex, gamma-R1 variant CPX-1118, Calcineurin-Calmodulin-AKAP5 complex, gamma-R2 variant |
IntActi | P48454, 35 interactors |
STRINGi | 9606.ENSP00000380878 |
Chemistry databases
BindingDBi | P48454 |
DrugCentrali | P48454 |
PTM databases
DEPODi | PPP3CC |
iPTMneti | P48454 |
PhosphoSitePlusi | P48454 |
Polymorphism and mutation databases
BioMutai | PPP3CC |
DMDMi | 257051041 |
Proteomic databases
EPDi | P48454 |
jPOSTi | P48454 |
MassIVEi | P48454 |
MaxQBi | P48454 |
PaxDbi | P48454 |
PeptideAtlasi | P48454 |
PRIDEi | P48454 |
ProteomicsDBi | 4973 55894 [P48454-1] 55895 [P48454-2] |
Protocols and materials databases
Antibodypediai | 9535, 214 antibodies |
DNASUi | 5533 |
Genome annotation databases
Ensembli | ENST00000240139; ENSP00000240139; ENSG00000120910 [P48454-1] ENST00000289963; ENSP00000289963; ENSG00000120910 [P48454-2] ENST00000397775; ENSP00000380878; ENSG00000120910 [P48454-3] |
GeneIDi | 5533 |
KEGGi | hsa:5533 |
UCSCi | uc003xbs.4, human [P48454-1] |
Organism-specific databases
CTDi | 5533 |
DisGeNETi | 5533 |
EuPathDBi | HostDB:ENSG00000120910.14 |
GeneCardsi | PPP3CC |
HGNCi | HGNC:9316, PPP3CC |
HPAi | ENSG00000120910, Low tissue specificity |
MIMi | 114107, gene |
neXtProti | NX_P48454 |
OpenTargetsi | ENSG00000120910 |
PharmGKBi | PA33680 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0375, Eukaryota |
GeneTreei | ENSGT00940000154115 |
InParanoidi | P48454 |
OMAi | ACMNTFD |
OrthoDBi | 463522at2759 |
PhylomeDBi | P48454 |
TreeFami | TF105557 |
Enzyme and pathway databases
PathwayCommonsi | P48454 |
Reactomei | R-HSA-111447, Activation of BAD and translocation to mitochondria R-HSA-180024, DARPP-32 events |
SIGNORi | P48454 |
Miscellaneous databases
BioGRID-ORCSi | 5533, 2 hits in 841 CRISPR screens |
ChiTaRSi | PPP3CC, human |
GeneWikii | PPP3CC |
GenomeRNAii | 5533 |
Pharosi | P48454, Tbio |
PROi | PR:P48454 |
RNActi | P48454, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000120910, Expressed in testis and 239 other tissues |
ExpressionAtlasi | P48454, baseline and differential |
Genevisiblei | P48454, HS |
Family and domain databases
CDDi | cd07416, MPP_PP2B, 1 hit |
Gene3Di | 3.60.21.10, 1 hit |
InterProi | View protein in InterPro IPR004843, Calcineurin-like_PHP_ApaH IPR029052, Metallo-depent_PP-like IPR041751, MPP_PP2B IPR043360, PP2B IPR006186, Ser/Thr-sp_prot-phosphatase |
PANTHERi | PTHR45673, PTHR45673, 1 hit |
Pfami | View protein in Pfam PF00149, Metallophos, 1 hit |
PRINTSi | PR00114, STPHPHTASE |
SMARTi | View protein in SMART SM00156, PP2Ac, 1 hit |
PROSITEi | View protein in PROSITE PS00125, SER_THR_PHOSPHATASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PP2BC_HUMAN | |
Accessioni | P48454Primary (citable) accession number: P48454 Secondary accession number(s): B4DRT5, Q9BSS6, Q9H4M5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1996 |
Last sequence update: | September 1, 2009 | |
Last modified: | December 2, 2020 | |
This is version 182 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 8
Human chromosome 8: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations