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Protein

Lanosterol synthase

Gene

Lss

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol nucleus. Through the production of lanosterol may regulate lens protein aggregation and increase transparency.By similarity1 Publication

Catalytic activityi

(3S)-2,3-epoxy-2,3-dihydrosqualene = lanosterol.1 Publication

Pathwayi: lanosterol biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes lanosterol from farnesyl diphosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Squalene synthase (Fdft1)
  2. Squalene monooxygenase (Sqle)
  3. Lanosterol synthase (Lss)
This subpathway is part of the pathway lanosterol biosynthesis, which is itself part of Terpene metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lanosterol from farnesyl diphosphate, the pathway lanosterol biosynthesis and in Terpene metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei233Proton acceptorBy similarity1
Active sitei456Proton donorBy similarity1

GO - Molecular functioni

  • lanosterol synthase activity Source: RGD

GO - Biological processi

  • cholesterol biosynthetic process Source: RGD
  • sterol metabolic process Source: RGD

Keywordsi

Molecular functionIsomerase
Biological processLipid biosynthesis, Lipid metabolism, Steroid biosynthesis

Enzyme and pathway databases

BRENDAi5.4.99.7 5301
SABIO-RKiP48450
UniPathwayi
UPA00767;UER00753

Names & Taxonomyi

Protein namesi
Recommended name:
Lanosterol synthase (EC:5.4.99.7)
Alternative name(s):
2,3-epoxysqualene--lanosterol cyclase
Oxidosqualene--lanosterol cyclase
Short name:
OSC
Gene namesi
Name:Lss
Synonyms:Osc
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620955 Lss

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Hypomorphic mutations in this gene and the Fdft1 gene were identified, as well as a null mutation in this gene. Cataract onset is associated with the specific combination of Lss and Fdft1 mutant alleles that decrease cholesterol levels in cataractous lenses to about 57% of normal. Cholesterol insufficiency may cause the deficient proliferation of lens epithelial cells in Shumiya cataract rats, resulting in the loss of homeostatic epithelial cell control of the underlying fiber cells and ultimately cataractogenesis.1 Publication

Chemistry databases

ChEMBLiCHEMBL3262
GuidetoPHARMACOLOGYi2434

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000726612 – 733Lanosterol synthaseAdd BLAST732

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiP48450

PTM databases

iPTMnetiP48450
PhosphoSitePlusiP48450

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi249578, 1 interactor

Chemistry databases

BindingDBiP48450

Structurei

3D structure databases

ProteinModelPortaliP48450
SMRiP48450
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati125 – 166PFTB 1Add BLAST42
Repeati484 – 529PFTB 2Add BLAST46
Repeati561 – 601PFTB 3Add BLAST41
Repeati613 – 654PFTB 4Add BLAST42

Sequence similaritiesi

Belongs to the terpene cyclase/mutase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000234317
HOVERGENiHBG005604
InParanoidiP48450
KOiK01852
PhylomeDBiP48450

Family and domain databases

CDDicd02892 SQCY_1, 1 hit
InterProiView protein in InterPro
IPR032696 SQ_cyclase_C
IPR032697 SQ_cyclase_N
IPR018333 Squalene_cyclase
IPR002365 Terpene_synthase_CS
IPR008930 Terpenoid_cyclase/PrenylTrfase
PfamiView protein in Pfam
PF13243 SQHop_cyclase_C, 1 hit
PF13249 SQHop_cyclase_N, 1 hit
SUPFAMiSSF48239 SSF48239, 2 hits
TIGRFAMsiTIGR01787 squalene_cyclas, 1 hit
PROSITEiView protein in PROSITE
PS01074 TERPENE_SYNTHASES, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.iShow all

P48450-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTEGTCLRRR GGPYKTEPAT DLTRWRLHNE LGRQRWTYYQ AEEDPGREQT
60 70 80 90 100
GLEAHSLGLD TTSYFKNLPK AQTAHEGALN GVTFYAKLQA EDGHWAGDYG
110 120 130 140 150
GPLFLLPGLL ITCHIAHIPL PAGYREEMVR YLRSVQLPDG GWGLHIEDKS
160 170 180 190 200
TVFGTALSYV SLRILGIGPD DPDLVRARNI LHKKGGAVAI PSWGKFWLAV
210 220 230 240 250
LNVYSWEGIN TLFPEMWLLP EWFPAHPSTL WCHCRQVYLP MSYCYATRLS
260 270 280 290 300
ASEDPLVQSL RQELYVEDYA SIDWPAQKNN VCPDDMYTPH SWLLHVVYGL
310 320 330 340 350
LNLYERFHST SLRKWAIQLL YEHVAADDRF TKCISIGPIS KTVNMLIRWS
360 370 380 390 400
VDGPSSPAFQ EHVSRIKDYL WLGLDGMKMQ GTNGSQTWDT SFAVQALLEA
410 420 430 440 450
GAHRRPEFLP CLQKAHEFLR LSQVPDNNPD YQKYYRHMHK GGFPFSTLDC
460 470 480 490 500
GWIVADCTAE ALKAVLLLQE RCPSITEHVP RERLYDAVAV LLSMRNSDGG
510 520 530 540 550
FATYETKRGG YLLELLNPSE VFGDIMIDYT YVECTSAVMQ ALRHFREYFP
560 570 580 590 600
DHRATESRET LNQGLDFCRK KQRADGSWEG SWGVCFTYGT WFGLEAFACM
610 620 630 640 650
GHIYQNRTAC AEVAQACHFL LSRQMADGGW GEDFESCEQR RYVQSAGSQV
660 670 680 690 700
HSTCWALLGL MAVRHPDISA QERGIRCLLG KQLPNGDWPQ ENISGVFNKS
710 720 730
CAISYTNYRN IFPIWALGRF SSLYPDNTLA GHI
Length:733
Mass (Da):83,301
Last modified:March 29, 2005 - v2
Checksum:i3679C0259CBDF771
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0G2JVC8A0A0G2JVC8_RAT
Terpene cyclase/mutase family membe...
Lss rCG_60576
733Annotation score:
A0A096MK62A0A096MK62_RAT
Lanosterol synthase
Lss
292Annotation score:
A0A096MK55A0A096MK55_RAT
Lanosterol synthase
Lss
338Annotation score:
A0A096MJH3A0A096MJH3_RAT
Lanosterol synthase
Lss
60Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti139D → N in AAA91023 (PubMed:7568116).Curated1
Sequence conflicti189A → G in BAA08208 (PubMed:7735243).Curated1
Sequence conflicti481R → Q in BAA08208 (PubMed:7735243).Curated1
Sequence conflicti486D → N in BAA08208 (PubMed:7735243).Curated1
Sequence conflicti557S → I in AAA91023 (PubMed:7568116).Curated1
Sequence conflicti683L → F in BAA08208 (PubMed:7735243).Curated1
Sequence conflicti687D → E in BAA08208 (PubMed:7735243).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31352 mRNA Translation: AAA91023.1
D45252 mRNA Translation: BAA08208.1
RefSeqiNP_112311.1, NM_031049.1
UniGeneiRn.10211

Genome annotation databases

GeneIDi81681
KEGGirno:81681
UCSCiRGD:620955 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31352 mRNA Translation: AAA91023.1
D45252 mRNA Translation: BAA08208.1
RefSeqiNP_112311.1, NM_031049.1
UniGeneiRn.10211

3D structure databases

ProteinModelPortaliP48450
SMRiP48450
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249578, 1 interactor

Chemistry databases

BindingDBiP48450
ChEMBLiCHEMBL3262
GuidetoPHARMACOLOGYi2434

PTM databases

iPTMnetiP48450
PhosphoSitePlusiP48450

Proteomic databases

PRIDEiP48450

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi81681
KEGGirno:81681
UCSCiRGD:620955 rat

Organism-specific databases

CTDi4047
RGDi620955 Lss

Phylogenomic databases

HOGENOMiHOG000234317
HOVERGENiHBG005604
InParanoidiP48450
KOiK01852
PhylomeDBiP48450

Enzyme and pathway databases

UniPathwayi
UPA00767;UER00753

BRENDAi5.4.99.7 5301
SABIO-RKiP48450

Miscellaneous databases

PROiPR:P48450

Family and domain databases

CDDicd02892 SQCY_1, 1 hit
InterProiView protein in InterPro
IPR032696 SQ_cyclase_C
IPR032697 SQ_cyclase_N
IPR018333 Squalene_cyclase
IPR002365 Terpene_synthase_CS
IPR008930 Terpenoid_cyclase/PrenylTrfase
PfamiView protein in Pfam
PF13243 SQHop_cyclase_C, 1 hit
PF13249 SQHop_cyclase_N, 1 hit
SUPFAMiSSF48239 SSF48239, 2 hits
TIGRFAMsiTIGR01787 squalene_cyclas, 1 hit
PROSITEiView protein in PROSITE
PS01074 TERPENE_SYNTHASES, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiERG7_RAT
AccessioniPrimary (citable) accession number: P48450
Secondary accession number(s): Q62811
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: March 29, 2005
Last modified: May 23, 2018
This is version 125 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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