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Protein

Transcription factor SOX-2

Gene

Sox2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity).By similarity2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi43 – 111HMG boxPROSITE-ProRule annotationAdd BLAST69

GO - Molecular functioni

  • chromatin binding Source: MGI
  • chromatin DNA binding Source: MGI
  • DNA binding Source: MGI
  • DNA binding transcription factor activity Source: UniProtKB
  • miRNA binding Source: MGI
  • protein heterodimerization activity Source: MGI
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: MGI
  • sequence-specific DNA binding Source: UniProtKB
  • transcription factor binding Source: UniProtKB
  • transcription regulatory region DNA binding Source: UniProtKB
  • transcription regulatory region sequence-specific DNA binding Source: MGI

GO - Biological processi

  • adenohypophysis development Source: MGI
  • anatomical structure formation involved in morphogenesis Source: MGI
  • cell cycle arrest Source: MGI
  • cell fate commitment Source: MGI
  • cell fate specification Source: MGI
  • cerebral cortex development Source: MGI
  • detection of mechanical stimulus involved in equilibrioception Source: MGI
  • detection of mechanical stimulus involved in sensory perception of sound Source: MGI
  • diencephalon morphogenesis Source: MGI
  • embryonic organ development Source: MGI
  • endodermal cell fate specification Source: MGI
  • epithelial tube branching involved in lung morphogenesis Source: MGI
  • forebrain neuron differentiation Source: MGI
  • inner ear morphogenesis Source: MGI
  • lens induction in camera-type eye Source: MGI
  • lung alveolus development Source: MGI
  • male genitalia development Source: MGI
  • negative regulation of canonical Wnt signaling pathway Source: UniProtKB
  • negative regulation of cell differentiation Source: MGI
  • negative regulation of epithelial cell proliferation Source: MGI
  • negative regulation of neuron differentiation Source: MGI
  • negative regulation of osteoblast differentiation Source: MGI
  • negative regulation of transcription by RNA polymerase II Source: MGI
  • negative regulation of Wnt signaling pathway Source: MGI
  • neuronal stem cell population maintenance Source: UniProtKB
  • neuron fate commitment Source: MGI
  • olfactory placode formation Source: MGI
  • osteoblast differentiation Source: MGI
  • pigment biosynthetic process Source: MGI
  • positive regulation of cell-cell adhesion Source: MGI
  • positive regulation of cell differentiation Source: MGI
  • positive regulation of epithelial cell differentiation Source: MGI
  • positive regulation of MAPK cascade Source: MGI
  • positive regulation of neuroblast proliferation Source: MGI
  • positive regulation of neuron differentiation Source: MGI
  • positive regulation of Notch signaling pathway Source: MGI
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription by RNA polymerase II Source: MGI
  • regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
  • regulation of gene expression Source: MGI
  • regulation of neurogenesis Source: MGI
  • regulation of transcription, DNA-templated Source: MGI
  • regulation of transcription by RNA polymerase II Source: MGI
  • response to growth factor Source: MGI
  • response to organic substance Source: MGI
  • response to retinoic acid Source: MGI
  • retina morphogenesis in camera-type eye Source: MGI
  • sensory perception of sound Source: MGI
  • somatic stem cell population maintenance Source: MGI
  • stem cell differentiation Source: MGI
  • stem cell population maintenance Source: UniProtKB
  • tongue development Source: MGI
  • transcription by RNA polymerase II Source: GOC

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor SOX-2
Gene namesi
Name:Sox2
Synonyms:Sox-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:98364 Sox2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Biotechnological usei

POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 are the four Yamanaka factors. When combined, these factors are sufficient to reprogram differentiated cells to an embryonic-like state designated iPS (induced pluripotent stem) cells. iPS cells exhibit the morphology and growth properties of ES cells and express ES cell marker genes.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi247K → R: Absence of sumoylation. Increased FGF4 activation. No effect on nuclear localization. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000487161 – 319Transcription factor SOX-2Add BLAST319

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki247Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
Modified residuei253PhosphoserineBy similarity1

Post-translational modificationi

Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP48432
PaxDbiP48432
PeptideAtlasiP48432
PRIDEiP48432

PTM databases

iPTMnetiP48432
PhosphoSitePlusiP48432

Expressioni

Tissue specificityi

Expressed in the brain and retina. A very low level expression is seen in the stomach and lung. Expressed in developing urogenital ridge.

Inductioni

By SRRT.1 Publication

Gene expression databases

CleanExiMM_SOX2

Interactioni

Subunit structurei

Interacts with ZSCAN10. Interacts with SOX3 and FGFR1.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein heterodimerization activity Source: MGI
  • transcription factor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi203406, 23 interactors
DIPiDIP-54522N
IntActiP48432, 8 interactors
MINTiP48432
STRINGi10090.ENSMUSP00000096755

Structurei

Secondary structure

1319
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi49 – 62Combined sources14
Helixi70 – 81Combined sources12
Helixi86 – 106Combined sources21

3D structure databases

ProteinModelPortaliP48432
SMRiP48432
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP48432

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi19 – 25Poly-Gly7

Phylogenomic databases

eggNOGiENOG410IPZI Eukaryota
ENOG411009V LUCA
HOGENOMiHOG000231647
HOVERGENiHBG105663
InParanoidiP48432
KOiK16796
PhylomeDBiP48432

Family and domain databases

Gene3Di1.10.30.10, 1 hit
InterProiView protein in InterPro
IPR009071 HMG_box_dom
IPR036910 HMG_box_dom_sf
IPR032643 SOX-2
IPR022097 SOX_fam
PANTHERiPTHR10270:SF231 PTHR10270:SF231, 1 hit
PfamiView protein in Pfam
PF00505 HMG_box, 1 hit
PF12336 SOXp, 1 hit
SMARTiView protein in SMART
SM00398 HMG, 1 hit
SUPFAMiSSF47095 SSF47095, 1 hit
PROSITEiView protein in PROSITE
PS50118 HMG_BOX_2, 1 hit

Sequencei

Sequence statusi: Complete.

P48432-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYNMMETELK PPGPQQASGG GGGGGNATAA ATGGNQKNSP DRVKRPMNAF
60 70 80 90 100
MVWSRGQRRK MAQENPKMHN SEISKRLGAE WKLLSETEKR PFIDEAKRLR
110 120 130 140 150
ALHMKEHPDY KYRPRRKTKT LMKKDKYTLP GGLLAPGGNS MASGVGVGAG
160 170 180 190 200
LGGGLNQRMD SYAHMNGWSN GSYSMMQEQL GYPQHPGLNA HGAAQMQPMH
210 220 230 240 250
RYVVSALQYN SMTSSQTYMN GSPTYSMSYS QQGTPGMALG SMGSVVKSEA
260 270 280 290 300
SSSPPVVTSS SHSRAPCQAG DLRDMISMYL PGAEVPEPAA PSRLHMAQHY
310
QSGPVPGTAK YGTLPLSHM
Length:319
Mass (Da):34,454
Last modified:July 15, 1999 - v2
Checksum:iC40DE49E524C49F1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti153 – 155GGL → AGV in CAA63847 (PubMed:8625802).Curated3
Sequence conflicti203V → D in CAA63847 (PubMed:8625802).Curated1
Sequence conflicti310 – 311KY → IN in CAA63847 (PubMed:8625802).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31967 mRNA Translation: AAC31791.1
X94127 Genomic DNA Translation: CAA63847.1
CCDSiCCDS38413.1
PIRiS10949
RefSeqiNP_035573.3, NM_011443.4
UniGeneiMm.65396

Genome annotation databases

GeneIDi20674
KEGGimmu:20674

Similar proteinsi

Entry informationi

Entry nameiSOX2_MOUSE
AccessioniPrimary (citable) accession number: P48432
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: July 15, 1999
Last modified: June 20, 2018
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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