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Protein

Flavonoid 3',5'-hydroxylase 1

Gene

CYP75A1

Organism
Petunia hybrida (Petunia)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the 3'5'-hydroxylation of naringenin and eriodictyol to form 5,7,3,'4',5'-pentahydroxyflavanone and 3',5'-hydroxylation of dihydrokaempferol and dihydroquercetin to form dihydromyricetin.1 Publication

Catalytic activityi

A flavanone + 2 [reduced NADPH--hemoprotein reductase] + 2 O2 = a 3',5'-dihydroxyflavanone + 2 [oxidized NADPH--hemoprotein reductase] + 2 H2O.1 Publication

Cofactori

hemeBy similarity

Pathwayi: anthocyanin biosynthesis

This protein is involved in the pathway anthocyanin biosynthesis, which is part of Pigment biosynthesis.
View all proteins of this organism that are known to be involved in the pathway anthocyanin biosynthesis and in Pigment biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi443Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
LigandHeme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12020
BRENDAi1.14.13.88 4700
UniPathwayiUPA00009

Names & Taxonomyi

Protein namesi
Recommended name:
Flavonoid 3',5'-hydroxylase 1 (EC:1.14.14.811 Publication)
Short name:
F3'5'H
Alternative name(s):
CYPLXXVA1
Cytochrome P450 75A1
Gene namesi
Name:CYP75A1
Synonyms:HF1
OrganismiPetunia hybrida (Petunia)
Taxonomic identifieri4102 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaePetunioideaePetunia

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000521291 – 506Flavonoid 3',5'-hydroxylase 1Add BLAST506

Proteomic databases

PRIDEiP48418

Expressioni

Tissue specificityi

Flowers.

Structurei

3D structure databases

ProteinModelPortaliP48418
SMRiP48418
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR017972 Cyt_P450_CS
IPR002401 Cyt_P450_E_grp-I
IPR036396 Cyt_P450_sf
PfamiView protein in Pfam
PF00067 p450, 1 hit
PRINTSiPR00463 EP450I
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit
PROSITEiView protein in PROSITE
PS00086 CYTOCHROME_P450, 1 hit

Sequencei

Sequence statusi: Complete.

P48418-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMLLTELGAA TSIFLIAHII ISTLISKTTG RHLPPGPRGW PVIGALPLLG
60 70 80 90 100
AMPHVSLAKM AKKYGAIMYL KVGTCGMAVA STPDAAKAFL KTLDINFSNR
110 120 130 140 150
PPNAGATHLA YNAQDMVFAH YGPRWKLLRK LSNLHMLGGK ALENWANVRA
160 170 180 190 200
NELGHMLKSM SDMSREGQRV VVAEMLTFAM ANMIGQVMLS KRVFVDKGVE
210 220 230 240 250
VNEFKDMVVE LMTIAGYFNI GDFIPCLAWM DLQGIEKRMK RLHKKFDALL
260 270 280 290 300
TKMFDEHKAT TYERKGKPDF LDVVMENGDN SEGERLSTTN IKALLLNLFT
310 320 330 340 350
AGTDTSSSAI EWALAEMMKN PAILKKAQAE MDQVIGRNRR LLESDIPNLP
360 370 380 390 400
YLRAICKETF RKHPSTPLNL PRISNEPCIV DGYYIPKNTR LSVNIWAIGR
410 420 430 440 450
DPQVWENPLE FNPERFLSGR NSKIDPRGND FELIPFGAGR RICAGTRMGI
460 470 480 490 500
VMVEYILGTL VHSFDWKLPS EVIELNMEEA FGLALQKAVP LEAMVTPRLQ

LDVYVP
Length:506
Mass (Da):56,548
Last modified:February 1, 1996 - v1
Checksum:iFCF3F9623BD82B3E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22545 mRNA Translation: CAA80266.1
D14588 mRNA Translation: BAA03438.1
AF081575 Genomic DNA Translation: AAC32274.1
AY245545 mRNA Translation: AAO91941.1
X71130 mRNA Translation: CAA50442.1
PIRiS32110
S38985

Similar proteinsi

Entry informationi

Entry nameiC75A1_PETHY
AccessioniPrimary (citable) accession number: P48418
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: July 18, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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