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Protein

Nonsense-mediated mRNA decay protein 3

Gene

UPF3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in nonsense-mediated decay of mRNAs containing premature stop codons.

Miscellaneous

Present with 1250 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • mRNA binding Source: GO_Central

GO - Biological processi

  • DNA recombination Source: SGD
  • nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay Source: SGD
  • nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: GO_Central
  • positive regulation of translation Source: GO_Central
  • protein ubiquitination Source: SGD

Keywordsi

Biological processNonsense-mediated mRNA decay

Enzyme and pathway databases

BioCyciYEAST:G3O-30785-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Nonsense-mediated mRNA decay protein 3
Alternative name(s):
Up-frameshift suppressor 3
Gene namesi
Name:UPF3
Synonyms:SUA6
Ordered Locus Names:YGR072W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

SGDiS000003304 UPF3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002152981 – 387Nonsense-mediated mRNA decay protein 3Add BLAST387

Proteomic databases

MaxQBiP48412
PaxDbiP48412
PRIDEiP48412

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
NMD2P387983EBI-20117,EBI-12071

Protein-protein interaction databases

BioGridi33315, 254 interactors
ComplexPortaliCPX-1315 Nonsense-mediated decay complex
DIPiDIP-2294N
IntActiP48412, 3 interactors
MINTiP48412
STRINGi4932.YGR072W

Structurei

3D structure databases

ProteinModelPortaliP48412
SMRiP48412
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi15 – 31Nuclear localization signalSequence analysisAdd BLAST17
Motifi58 – 75Nuclear localization signalSequence analysisAdd BLAST18
Motifi284 – 300Nuclear localization signalSequence analysisAdd BLAST17

Sequence similaritiesi

Belongs to the RENT3 family.Curated

Phylogenomic databases

InParanoidiP48412
KOiK14328
OMAiLSPYVKK
OrthoDBiEOG092C5M8L

Family and domain databases

Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR039722 Upf3
IPR005120 UPF3_dom
PANTHERiPTHR13112 PTHR13112, 1 hit
PfamiView protein in Pfam
PF03467 Smg4_UPF3, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit

Sequencei

Sequence statusi: Complete.

P48412-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSNVAGELKN SEGKKKGRGN RYHNKNRGKS KNETVDPKKN ENKVNNATNA
60 70 80 90 100
THNNSKGRRN NKKRNREYYN YKRKARLGKS TENEGFKLVI RLLPPNLTAD
110 120 130 140 150
EFFAILRDNN NDDGDKQDIQ GKLKYSDWCF FEGHYSSKVF KNSTYSRCNF
160 170 180 190 200
LFDNLSDLEK CANFIKTCKF IDNKDNITIP DMKLSPYVKK FTQTSKKDAA
210 220 230 240 250
LVGTIEEDEI FKTFMNSMKQ LNENDEYSFQ DFSVLKSLEK EFSKSIELEN
260 270 280 290 300
KIAERTERVL TELVGTGDKV KNKNKKKKNK NAKKKFKEEE ASAKIPKKKR
310 320 330 340 350
NRGKKKRENR EKSTISKTKN SNVVIIEEAG KEVLKQRKKK MLLQEKLKIS
360 370 380
NSSQPQSSSA QTQPSFQPKE NLFVPRVKIL HRDDTKK
Length:387
Mass (Da):44,924
Last modified:February 1, 1996 - v1
Checksum:i665A572D9A393133
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26N → D in AAT92769 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L41153 Genomic DNA Translation: AAC41671.1
Z72857 Genomic DNA Translation: CAA97074.1
AY692750 Genomic DNA Translation: AAT92769.1
BK006941 Genomic DNA Translation: DAA08166.1
PIRiS62140
RefSeqiNP_011586.1, NM_001181201.1

Genome annotation databases

EnsemblFungiiYGR072W; YGR072W; YGR072W
GeneIDi852963
KEGGisce:YGR072W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L41153 Genomic DNA Translation: AAC41671.1
Z72857 Genomic DNA Translation: CAA97074.1
AY692750 Genomic DNA Translation: AAT92769.1
BK006941 Genomic DNA Translation: DAA08166.1
PIRiS62140
RefSeqiNP_011586.1, NM_001181201.1

3D structure databases

ProteinModelPortaliP48412
SMRiP48412
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33315, 254 interactors
ComplexPortaliCPX-1315 Nonsense-mediated decay complex
DIPiDIP-2294N
IntActiP48412, 3 interactors
MINTiP48412
STRINGi4932.YGR072W

Proteomic databases

MaxQBiP48412
PaxDbiP48412
PRIDEiP48412

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR072W; YGR072W; YGR072W
GeneIDi852963
KEGGisce:YGR072W

Organism-specific databases

SGDiS000003304 UPF3

Phylogenomic databases

InParanoidiP48412
KOiK14328
OMAiLSPYVKK
OrthoDBiEOG092C5M8L

Enzyme and pathway databases

BioCyciYEAST:G3O-30785-MONOMER

Miscellaneous databases

PROiPR:P48412

Family and domain databases

Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR039722 Upf3
IPR005120 UPF3_dom
PANTHERiPTHR13112 PTHR13112, 1 hit
PfamiView protein in Pfam
PF03467 Smg4_UPF3, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiUPF3_YEAST
AccessioniPrimary (citable) accession number: P48412
Secondary accession number(s): D6VUK5, E9P8W8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 7, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names
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