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Protein

DNA gyrase subunit A

Gene

gyrA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.UniRule annotation

Miscellaneous

Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.UniRule annotation

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei122O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Isomerase, Topoisomerase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPAER208964:G1FZ6-3228-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA gyrase subunit AUniRule annotation (EC:5.99.1.3UniRule annotation)
Gene namesi
Name:gyrAUniRule annotation
Ordered Locus Names:PA3168
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA3168

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3390828

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001452451 – 923DNA gyrase subunit AAdd BLAST923

Proteomic databases

PaxDbiP48372
PRIDEiP48372

Interactioni

Subunit structurei

Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.UniRule annotation

Protein-protein interaction databases

STRINGi208964.PA3168

Structurei

3D structure databases

ProteinModelPortaliP48372
SMRiP48372
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi561 – 567GyrA-boxUniRule annotation7

Sequence similaritiesi

Belongs to the type II topoisomerase GyrA/ParC subunit family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C24 Bacteria
COG0188 LUCA
HOGENOMiHOG000076278
InParanoidiP48372
KOiK02469
OMAiTHHWLLF
PhylomeDBiP48372

Family and domain databases

Gene3Di1.10.268.10, 1 hit
3.90.199.10, 3 hits
HAMAPiMF_01897 GyrA, 1 hit
InterProiView protein in InterPro
IPR005743 GyrA
IPR006691 GyrA/parC_rep
IPR035516 Gyrase/topoIV_suA_C
IPR013760 Topo_IIA-like_dom_sf
IPR002205 Topo_IIA_A/C
IPR013758 Topo_IIA_A/C_ab
IPR013757 Topo_IIA_A_a_sf
PfamiView protein in Pfam
PF03989 DNA_gyraseA_C, 6 hits
PF00521 DNA_topoisoIV, 1 hit
SMARTiView protein in SMART
SM00434 TOP4c, 1 hit
SUPFAMiSSF101904 SSF101904, 2 hits
SSF56719 SSF56719, 1 hit

Sequencei

Sequence statusi: Complete.

P48372-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGELAKEILP VNIEDELKQS YLDYAMSVIV GRALPDARDG LKPVHRRVLY
60 70 80 90 100
AMSELGNDWN KPYKKSARVV GDVIGKYHPH GDTAVYDTIV RMAQPFSLRY
110 120 130 140 150
MLVDGQGNFG SVDGDNAAAM RYTEVRMAKL AHELLADLEK ETVDWVPNYD
160 170 180 190 200
GTEQIPAVMP TKIPNLLVNG SSGIAVGMAT NIPPHNLGEV IDGCLALMDN
210 220 230 240 250
PDLTVDELMQ YIPGPDFPTA GIINGRAGII EAYRTGRGRI YIRARAVVEE
260 270 280 290 300
MEKGGGREQI IITELPYQLN KARLIEKIAE LVKEKKIEGI SELRDESDKD
310 320 330 340 350
GMRVVIELRR GEVGEVVLNN LYAQTQLQSV FGINVVALVD GQPRTLNLKD
360 370 380 390 400
MLEVFVRHRR EVVTRRTVYE LRKARERGHI LEGQAVALSN IDPVIELIKS
410 420 430 440 450
SPTPAEAKER LIATAWESSA VEAMVERAGA DACRPEDLDP QYGLRDGKYY
460 470 480 490 500
LSPEQAQAIL ELRLHRLTGL EHEKLLSEYQ EILNLIGELI RILTNPARLM
510 520 530 540 550
EVIREELEAV KAEFGDARRT EIVASQVDLT IADLITEEDR VVTISHGGYA
560 570 580 590 600
KSQPLAAYQA QRRGGKGKSA TGMKDEDYIE HLLVANSHAT LLLFSSKGKV
610 620 630 640 650
YWLRTFEIPE ASRTARGRPL VNLLPLDEGE RITAMLQIDL EALQQNGGAD
660 670 680 690 700
DDLDEAEGAV LEGEVVEAAE VEEVEGETAE LVAEPTGAYI FMATAFGTVK
710 720 730 740 750
KTPLVQFSRP RSSGLIALKL EEGDTLIAAA ITDGAKEVML FSSAGKVIRF
760 770 780 790 800
AESVVRIMGR NARGVRGMRL GKGQQLISML IPESGAQILT ASERGFGKRT
810 820 830 840 850
PLSKFPRRGR GGQGVIAMVT NERNGALIAA VQVQEGEEIM LISDQGTLVR
860 870 880 890 900
TRVDEVSLSG RNTQGVTLIK LASDEVLVGL ERVQEPSGGD DEDLPEGEEA
910 920
AESLGESAES ESEPAAEAEG NEE
Length:923
Mass (Da):101,135
Last modified:February 1, 1996 - v1
Checksum:i0025DFE08CDD1BEC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L29417 Genomic DNA Translation: AAA68625.1
AE004091 Genomic DNA Translation: AAG06556.1
PIRiH83248
RefSeqiNP_251858.1, NC_002516.2
WP_003091448.1, NC_002516.2

Genome annotation databases

EnsemblBacteriaiAAG06556; AAG06556; PA3168
GeneIDi882800
KEGGipae:PA3168
PATRICifig|208964.12.peg.3311

Similar proteinsi

Entry informationi

Entry nameiGYRA_PSEAE
AccessioniPrimary (citable) accession number: P48372
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: February 28, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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