Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Malate dehydrogenase

Gene

mdh

Organism
Moritella sp. (strain 5710)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.1 Publication

pH dependencei

Optimum pH is 9.5-10.0 for malate dehydrogenation, and 7.5-8.0 for oxaloacetate reduction.

Temperature dependencei

Optimum temperature is 35 degrees Celsius.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei34NADBy similarity1
Binding sitei81SubstrateBy similarity1
Binding sitei87SubstrateBy similarity1
Binding sitei94NADBy similarity1
Binding sitei119SubstrateBy similarity1
Binding sitei153SubstrateBy similarity1
Active sitei177Proton acceptorBy similarity1
Binding sitei227NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi7 – 13NADBy similarity7
Nucleotide bindingi117 – 119NADBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processTricarboxylic acid cycle
LigandNAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase (EC:1.1.1.37)
Gene namesi
Name:mdh
OrganismiMoritella sp. (strain 5710)
Taxonomic identifieri246794 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesMoritellaceaeMoritella

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi229Q → H: Increases the thermal stability. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001133161 – 312Malate dehydrogenaseAdd BLAST312

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP48364
SMRiP48364
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_01516 Malate_dehydrog_1, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR001252 Malate_DH_AS
IPR010097 Malate_DH_type1
IPR023958 Malate_DH_type1_bac
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR11540:SF22 PTHR11540:SF22, 1 hit
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01772 MDH_euk_gproteo, 1 hit
PROSITEiView protein in PROSITE
PS00068 MDH, 1 hit

Sequencei

Sequence statusi: Complete.

P48364-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKVAVLGAAG GIGQALALLL KTQLPAGSDL SLYDIAPVTP GVAVDLSHIP
60 70 80 90 100
TDVTIAGFAG MDPTDALVGA DVVLISAGVA RKPGMDRSDL FNINAGIIKN
110 120 130 140 150
LAGKCAEVCP NACIGIITNP VNTTVPIAAE VLKQAGVYDK RKLFGITTLD
160 170 180 190 200
VIRSETFVSA LKGISLADVE VPVIGGHSGV TILPLLSQVK GVEFTAEEVV
210 220 230 240 250
ALTARIQNAG TEVVEAKAGG GSATLSMGQA AARFGLSLVR ALQGEKGIVE
260 270 280 290 300
CTYVDGGSEH ATFFAQPVLL GKNGVEEVLA YGELSEFETN ARDAMLEELK
310
ANITLGEEFV AG
Length:312
Mass (Da):31,985
Last modified:February 1, 1996 - v1
Checksum:i2C4A3253E5B5D2E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78194 Genomic DNA Translation: BAA11301.1

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78194 Genomic DNA Translation: BAA11301.1

3D structure databases

ProteinModelPortaliP48364
SMRiP48364
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_01516 Malate_dehydrog_1, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR001252 Malate_DH_AS
IPR010097 Malate_DH_type1
IPR023958 Malate_DH_type1_bac
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR11540:SF22 PTHR11540:SF22, 1 hit
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01772 MDH_euk_gproteo, 1 hit
PROSITEiView protein in PROSITE
PS00068 MDH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMDH_MORS5
AccessioniPrimary (citable) accession number: P48364
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 10, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again