We will be switching to the new UniProt website on Tuesday, June 28. Please explore and share your feedback.
Take me to UniProt BETA
UniProtKB - P48239 (GTO1_YEAST)
Protein
Glutathione S-transferase omega-like 1
Gene
GTO1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Active as '1-Cys' thiol transferase against beta-hydroxyethyl disulfide (HED), as dehydroascorbate reductase and as dimethylarsinic acid reductase, while not active against the standard GST substrate 1-chloro-2,4-dinitrobenzene (CDNB).
1 PublicationCatalytic activityi
Kineticsi
- KM=1.13 mM for reduced glutathione (GSH)1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 31 | NucleophileBy similarity | 1 |
GO - Molecular functioni
- glutathione dehydrogenase (ascorbate) activity Source: UniProtKB-EC
- glutathione transferase activity Source: SGD
GO - Biological processi
- glutathione metabolic process Source: SGD
Keywordsi
Molecular function | Oxidoreductase, Transferase |
Enzyme and pathway databases
SABIO-RKi | P48239 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:GTO1 Ordered Locus Names:YGR154C ORF Names:G6664 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003386, GTO1 |
VEuPathDBi | FungiDB:YGR154C |
Subcellular locationi
Peroxisome
- Peroxisome 1 Publication
Peroxisome
- peroxisome Source: SGD
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
PeroxisomePathology & Biotechi
Disruption phenotypei
Null mutants display increased sensitivity to cadmium in the absence of GTT1 and GTT2. Null mutants also show growth defects on oleic acid-based medium, indicative of abnormal peroxisomal functions and altered expression of genes related to sulfur amino acid metabolism, and have low levels of reduced glutathione.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000202833 | 1 – 356 | Glutathione S-transferase omega-like 1Add BLAST | 356 |
Proteomic databases
PaxDbi | P48239 |
PRIDEi | P48239 |
Expressioni
Inductioni
By diamide and 1-chloro-2,4-dinitrobenzene (CDNB) in a transcriptional factors YAP1 and/or MSN2/4-dependent manner.1 Publication
Interactioni
Protein-protein interaction databases
BioGRIDi | 33402, 77 interactors |
DIPi | DIP-1867N |
IntActi | P48239, 2 interactors |
MINTi | P48239 |
STRINGi | 4932.YGR154C |
Miscellaneous databases
RNActi | P48239, protein |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 214 – 257 | Sequence analysisAdd BLAST | 44 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG2903, Eukaryota |
GeneTreei | ENSGT00530000065151 |
HOGENOMi | CLU_037263_0_1_1 |
InParanoidi | P48239 |
OMAi | AHQPYLF |
Family and domain databases
InterProi | View protein in InterPro IPR036282, Glutathione-S-Trfase_C_sf IPR004045, Glutathione_S-Trfase_N IPR016639, GST_Omega/GSH IPR036249, Thioredoxin-like_sf |
PANTHERi | PTHR32419, PTHR32419, 1 hit |
Pfami | View protein in Pfam PF13409, GST_N_2, 1 hit |
PIRSFi | PIRSF015753, GST, 1 hit |
SUPFAMi | SSF47616, SSF47616, 1 hit SSF52833, SSF52833, 1 hit |
i Sequence
Sequence statusi: Complete.
P48239-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSVSYKGTIS KTHSVFKPEK GRYYIYGALG CPFTHRAILA RSLKKLEPVL
60 70 80 90 100
GLVLSHWQLD SKGARFLPAP HRPEKYKERF FTATGGIASA KLDESEELGD
110 120 130 140 150
VNNDSARLFV DGAFDPVENI SRLSELYYLN DPKYPGTKFT VPVLWDSKTR
160 170 180 190 200
KIVNNESGDI IRILNSGVFD EFIQSEETNV IDLVPHDLID EIDKNIKWVH
210 220 230 240 250
PKINLGVYKV GLAENGKIYE TEVKTLFENL QKMECVLKEN YKRLEEQFSG
260 270 280 290 300
NKQKILAKYF VLGQRLTEAD IRLYPSIIRF DVVYVQHFKC NLKTIRDGFP
310 320 330 340 350
YLHLWLINLY WNYAEFRFTT DFNHIKLFYI RMEVSRNKIN QFGIVPLGPK
PDISRL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X85807 Genomic DNA Translation: CAA59811.1 Z72939 Genomic DNA Translation: CAA97168.1 BK006941 Genomic DNA Translation: DAA08245.1 |
PIRi | S60444 |
RefSeqi | NP_011670.3, NM_001181283.3 |
Genome annotation databases
EnsemblFungii | YGR154C_mRNA; YGR154C; YGR154C |
GeneIDi | 853058 |
KEGGi | sce:YGR154C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X85807 Genomic DNA Translation: CAA59811.1 Z72939 Genomic DNA Translation: CAA97168.1 BK006941 Genomic DNA Translation: DAA08245.1 |
PIRi | S60444 |
RefSeqi | NP_011670.3, NM_001181283.3 |
3D structure databases
AlphaFoldDBi | P48239 |
SMRi | P48239 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 33402, 77 interactors |
DIPi | DIP-1867N |
IntActi | P48239, 2 interactors |
MINTi | P48239 |
STRINGi | 4932.YGR154C |
Proteomic databases
PaxDbi | P48239 |
PRIDEi | P48239 |
Genome annotation databases
EnsemblFungii | YGR154C_mRNA; YGR154C; YGR154C |
GeneIDi | 853058 |
KEGGi | sce:YGR154C |
Organism-specific databases
SGDi | S000003386, GTO1 |
VEuPathDBi | FungiDB:YGR154C |
Phylogenomic databases
eggNOGi | KOG2903, Eukaryota |
GeneTreei | ENSGT00530000065151 |
HOGENOMi | CLU_037263_0_1_1 |
InParanoidi | P48239 |
OMAi | AHQPYLF |
Enzyme and pathway databases
SABIO-RKi | P48239 |
Miscellaneous databases
PROi | PR:P48239 |
RNActi | P48239, protein |
Family and domain databases
InterProi | View protein in InterPro IPR036282, Glutathione-S-Trfase_C_sf IPR004045, Glutathione_S-Trfase_N IPR016639, GST_Omega/GSH IPR036249, Thioredoxin-like_sf |
PANTHERi | PTHR32419, PTHR32419, 1 hit |
Pfami | View protein in Pfam PF13409, GST_N_2, 1 hit |
PIRSFi | PIRSF015753, GST, 1 hit |
SUPFAMi | SSF47616, SSF47616, 1 hit SSF52833, SSF52833, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GTO1_YEAST | |
Accessioni | P48239Primary (citable) accession number: P48239 Secondary accession number(s): D6VUT4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1996 |
Last sequence update: | February 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 146 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VII
Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names - SIMILARITY comments
Index of protein domains and families