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Protein

Protein 4.1

Gene

Epb41

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protein 4.1 is a major structural element of the erythrocyte membrane skeleton. It plays a key role in regulating membrane physical properties of mechanical stability and deformability by stabilizing spectrin-actin interaction. Recruits DLG1 to membranes. Required for dynein-dynactin complex and NUMA1 recruitment at the mitotic cell cortex during anaphase.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding, Calmodulin-binding
Biological processCell cycle, Cell division, Mitosis, Transport

Enzyme and pathway databases

ReactomeiR-MMU-6794361 Neurexins and neuroligins

Names & Taxonomyi

Protein namesi
Recommended name:
Protein 4.1
Short name:
P4.1
Alternative name(s):
4.1R
Band 4.1
Gene namesi
Name:Epb41
Synonyms:Epb4.1, Kiaa4056
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:95401 Epb41

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002193911 – 858Protein 4.1Add BLAST858

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14PhosphoserineBy similarity1
Modified residuei62PhosphothreonineBy similarity1
Modified residuei86PhosphoserineBy similarity1
Modified residuei87PhosphoserineBy similarity1
Modified residuei97PhosphoserineBy similarity1
Modified residuei106PhosphoserineBy similarity1
Modified residuei123PhosphoserineCombined sources1
Modified residuei151PhosphoserineBy similarity1
Modified residuei153PhosphoserineBy similarity1
Modified residuei154PhosphoserineBy similarity1
Modified residuei192PhosphoserineBy similarity1
Modified residuei223PhosphotyrosineCombined sources1
Modified residuei379PhosphothreonineBy similarity1
Modified residuei522PhosphoserineBy similarity1
Modified residuei541PhosphoserineCombined sources1
Modified residuei543PhosphoserineCombined sources1
Modified residuei556PhosphoserineCombined sources1
Modified residuei654PhosphotyrosineBy similarity1
Modified residuei658PhosphoserineCombined sources1
Modified residuei668PhosphoserineCombined sources1
Modified residuei678PhosphoserineBy similarity1
Modified residuei703PhosphoserineBy similarity1
Modified residuei706PhosphoserineBy similarity1
Modified residuei730PhosphothreonineBy similarity1
Modified residuei853PhosphothreonineBy similarity1

Post-translational modificationi

O-glycosylated; contains N-acetylglucosamine side chains in the C-terminal domain.By similarity
Phosphorylated at multiple sites by different protein kinases and each phosphorylation event selectively modulates the protein's functions.
Phosphorylation on Tyr-654 reduces the ability of 4.1 to promote the assembly of the spectrin/actin/4.1 ternary complex.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP48193
PaxDbiP48193
PeptideAtlasiP48193
PRIDEiP48193

PTM databases

iPTMnetiP48193
PhosphoSitePlusiP48193

Expressioni

Gene expression databases

BgeeiENSMUSG00000028906 Expressed in 268 organ(s), highest expression level in blood
CleanExiMM_EPB4.1
ExpressionAtlasiP48193 baseline and differential
GenevisibleiP48193 MM

Interactioni

Subunit structurei

Binds with a high affinity to glycophorin and with lower affinity to band III protein. Associates with the nuclear mitotic apparatus. Binds calmodulin, CENPJ and DLG1. Also found to associate with contractile apparatus and tight junctions. Interacts with NUMA1; this interaction is negatively regulated by CDK1 during metaphase and promotes anaphase-specific localization of NUMA1 in symmetrically dividing cells.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiP48193, 3 interactors
MINTiP48193
STRINGi10090.ENSMUSP00000030739

Structurei

3D structure databases

ProteinModelPortaliP48193
SMRiP48193
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini211 – 492FERMPROSITE-ProRule annotationAdd BLAST282

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni495 – 608HydrophilicAdd BLAST114
Regioni609 – 707Spectrin--actin-bindingAdd BLAST99
Regioni710 – 858C-terminal (CTD)Add BLAST149

Phylogenomic databases

eggNOGiENOG410KDUP Eukaryota
ENOG410XS0M LUCA
GeneTreeiENSGT00760000118823
HOGENOMiHOG000228841
HOVERGENiHBG007777
InParanoidiP48193
KOiK06107

Family and domain databases

CDDicd14473 FERM_B-lobe, 1 hit
Gene3Di1.20.80.10, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR008379 Band_4.1_C
IPR019749 Band_41_domain
IPR021187 Band_41_protein_chordates
IPR000798 Ez/rad/moesin-like
IPR014847 FERM-adjacent
IPR014352 FERM/acyl-CoA-bd_prot_sf
IPR035963 FERM_2
IPR019748 FERM_central
IPR019747 FERM_CS
IPR000299 FERM_domain
IPR018979 FERM_N
IPR018980 FERM_PH-like_C
IPR011993 PH-like_dom_sf
IPR007477 SAB_dom
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR23280:SF12 PTHR23280:SF12, 2 hits
PfamiView protein in Pfam
PF05902 4_1_CTD, 1 hit
PF08736 FA, 1 hit
PF09380 FERM_C, 1 hit
PF00373 FERM_M, 1 hit
PF09379 FERM_N, 1 hit
PF04382 SAB, 1 hit
PRINTSiPR00935 BAND41
PR00661 ERMFAMILY
SMARTiView protein in SMART
SM00295 B41, 1 hit
SM01195 FA, 1 hit
SM01196 FERM_C, 1 hit
SUPFAMiSSF47031 SSF47031, 1 hit
SSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS00660 FERM_1, 1 hit
PS00661 FERM_2, 1 hit
PS50057 FERM_3, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Note: A number of isoforms are produced.

This entry has 2 described isoforms and 9 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P48193-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTTEKSLAAE AENSQHQQQK EEGEGATNSG QQETQLEEAS QAAAAEGSDQ
60 70 80 90 100
GEQKLKASNG DTPTHEDLTK NKERTSESRG LSRLLSSFLK RPKSQVSEEE
110 120 130 140 150
GREVESEKEK GEGGQKEIEL GNSLDEDIIL KAPIAAPEPE LKTDPSLDLH
160 170 180 190 200
SLSSIETQPA QEEHREDPDS ETKEGEGIEE CSGTEVKEDP ESRAEREPEA
210 220 230 240 250
SQKPVRRHRN MHCKVSLLDD TVYECVVEKH AKGQDLLKRV CEHLNLLEED
260 270 280 290 300
YFGLALWDSA TSKTWLDSAK EIKKQVRGVP WNFTFNVKFY PPDPAQLTED
310 320 330 340 350
ITRYYLCLQL RQDIVAGRLP CSFATLALLG SYTIQSELGD YDPELHGMDY
360 370 380 390 400
VSDFKLAPNQ TKELEEKVME LHKSYRSMTP AQADLEFLEN AKKLSMYGVD
410 420 430 440 450
LHKAKDLEGV DIILGVCSSG LLVYKDKLRI NRFPWPKVLK ISYKRSSFFI
460 470 480 490 500
KIRPGEQEHY ESTIGFKLPS YRAAKKLWKV CVEHHTFFRL TSTDTIPKSK
510 520 530 540 550
FLALGSKFRY SGRTQAQTRQ ASALIDRPAP HFERTASKRA SRSLDGAAAA
560 570 580 590 600
ESTDRSPRPT SAPAIAQSQV TEGPGAPIKK TPKEAVKVEE KRGEEPAEPA
610 620 630 640 650
EPEPTEAWKV EKTHTEVTVP TSNGDQTQKL AGKGEDLIRM RKKKRERLDG
660 670 680 690 700
ENIYIRHSNL MLEDLDKSQE EIKKHHASIS ELKKNFMESV PEPRPSEWDK
710 720 730 740 750
RLSTHSPFRT LNINGQVPTG DGPPLVKTQT VTISDTANAV KSEIPTKDVP
760 770 780 790 800
IVHTETKTIT YEAAQTEDSN GDLDPGVLLT AQTITSETTS STTTTQITKT
810 820 830 840 850
VKGGISETRI EKRIVITGDA DIDHDQVLVQ AIKEAKEQHP DMSVTKVVVH

QETEISEE
Length:858
Mass (Da):95,911
Last modified:May 29, 2007 - v2
Checksum:i7CF1CD52D790D1FD
GO
Isoform 2 (identifier: P48193-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     610-663: Missing.

Show »
Length:804
Mass (Da):89,664
Checksum:iB4BCAD3F40CB7864
GO

Computationally mapped potential isoform sequencesi

There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6S4K9F6S4K9_MOUSE
Protein 4.1
Epb41 Epb4.1
375Annotation score:
F7CR30F7CR30_MOUSE
Protein 4.1
Epb41 Epb4.1
197Annotation score:
A2A841A2A841_MOUSE
Protein 4.1
Epb41 Epb4.1
869Annotation score:
A0A1D5RLV1A0A1D5RLV1_MOUSE
Protein 4.1
Epb41
476Annotation score:
A2A838A2A838_MOUSE
Protein 4.1
Epb41 Epb4.1
667Annotation score:
A2A842A2A842_MOUSE
Protein 4.1
Epb41 Epb4.1
769Annotation score:
A2A839A2A839_MOUSE
Protein 4.1
Epb41 Epb4.1
639Annotation score:
F7BUB8F7BUB8_MOUSE
Protein 4.1
Epb41 Epb4.1
250Annotation score:
A2AD32A2AD32_MOUSE
Protein 4.1
Epb41 Epb4.1
715Annotation score:

Sequence cautioni

The sequence BAD90280 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1 – 3MTT → EPLKGREPRRARTRPGPARP GPCQVPVLCSP in AAH68138 (PubMed:15489334).Curated3
Sequence conflicti8A → V in AAA37123 (PubMed:8429050).Curated1
Sequence conflicti232 – 233KG → NL in AAA37122 (PubMed:8429050).Curated2
Sequence conflicti443Y → S in AAA37122 (PubMed:8429050).Curated1
Sequence conflicti576A → R in AAA37122 (PubMed:8429050).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_012874610 – 663Missing in isoform 2. 2 PublicationsAdd BLAST54

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00919 mRNA Translation: AAA37122.1
L00919 mRNA Translation: AAA37123.1
AK220462 mRNA Translation: BAD90280.1 Different initiation.
AL607088, AL669981 Genomic DNA Translation: CAM14971.1
AL669981, AL607088 Genomic DNA Translation: CAM21329.1
BC068138 mRNA Translation: AAH68138.1
BC079875 mRNA Translation: AAH79875.1
CCDSiCCDS18717.1 [P48193-1]
CCDS51317.1 [P48193-2]
PIRiA46613
RefSeqiNP_001122078.1, NM_001128606.1 [P48193-2]
NP_001122079.1, NM_001128607.1
NP_906273.3, NM_183428.3 [P48193-1]
UniGeneiMm.30038
Mm.471627

Genome annotation databases

EnsembliENSMUST00000030739; ENSMUSP00000030739; ENSMUSG00000028906 [P48193-1]
ENSMUST00000054917; ENSMUSP00000060375; ENSMUSG00000028906 [P48193-2]
ENSMUST00000084253; ENSMUSP00000081274; ENSMUSG00000028906 [P48193-2]
ENSMUST00000105972; ENSMUSP00000101592; ENSMUSG00000028906 [P48193-1]
ENSMUST00000105981; ENSMUSP00000101601; ENSMUSG00000028906 [P48193-1]
GeneIDi269587
KEGGimmu:269587
UCSCiuc008val.3 mouse [P48193-2]
uc008vam.3 mouse [P48193-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00919 mRNA Translation: AAA37122.1
L00919 mRNA Translation: AAA37123.1
AK220462 mRNA Translation: BAD90280.1 Different initiation.
AL607088, AL669981 Genomic DNA Translation: CAM14971.1
AL669981, AL607088 Genomic DNA Translation: CAM21329.1
BC068138 mRNA Translation: AAH68138.1
BC079875 mRNA Translation: AAH79875.1
CCDSiCCDS18717.1 [P48193-1]
CCDS51317.1 [P48193-2]
PIRiA46613
RefSeqiNP_001122078.1, NM_001128606.1 [P48193-2]
NP_001122079.1, NM_001128607.1
NP_906273.3, NM_183428.3 [P48193-1]
UniGeneiMm.30038
Mm.471627

3D structure databases

ProteinModelPortaliP48193
SMRiP48193
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP48193, 3 interactors
MINTiP48193
STRINGi10090.ENSMUSP00000030739

PTM databases

iPTMnetiP48193
PhosphoSitePlusiP48193

Proteomic databases

MaxQBiP48193
PaxDbiP48193
PeptideAtlasiP48193
PRIDEiP48193

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030739; ENSMUSP00000030739; ENSMUSG00000028906 [P48193-1]
ENSMUST00000054917; ENSMUSP00000060375; ENSMUSG00000028906 [P48193-2]
ENSMUST00000084253; ENSMUSP00000081274; ENSMUSG00000028906 [P48193-2]
ENSMUST00000105972; ENSMUSP00000101592; ENSMUSG00000028906 [P48193-1]
ENSMUST00000105981; ENSMUSP00000101601; ENSMUSG00000028906 [P48193-1]
GeneIDi269587
KEGGimmu:269587
UCSCiuc008val.3 mouse [P48193-2]
uc008vam.3 mouse [P48193-1]

Organism-specific databases

CTDi2035
MGIiMGI:95401 Epb41
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410KDUP Eukaryota
ENOG410XS0M LUCA
GeneTreeiENSGT00760000118823
HOGENOMiHOG000228841
HOVERGENiHBG007777
InParanoidiP48193
KOiK06107

Enzyme and pathway databases

ReactomeiR-MMU-6794361 Neurexins and neuroligins

Miscellaneous databases

ChiTaRSiEpb41 mouse
PROiPR:P48193
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028906 Expressed in 268 organ(s), highest expression level in blood
CleanExiMM_EPB4.1
ExpressionAtlasiP48193 baseline and differential
GenevisibleiP48193 MM

Family and domain databases

CDDicd14473 FERM_B-lobe, 1 hit
Gene3Di1.20.80.10, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR008379 Band_4.1_C
IPR019749 Band_41_domain
IPR021187 Band_41_protein_chordates
IPR000798 Ez/rad/moesin-like
IPR014847 FERM-adjacent
IPR014352 FERM/acyl-CoA-bd_prot_sf
IPR035963 FERM_2
IPR019748 FERM_central
IPR019747 FERM_CS
IPR000299 FERM_domain
IPR018979 FERM_N
IPR018980 FERM_PH-like_C
IPR011993 PH-like_dom_sf
IPR007477 SAB_dom
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR23280:SF12 PTHR23280:SF12, 2 hits
PfamiView protein in Pfam
PF05902 4_1_CTD, 1 hit
PF08736 FA, 1 hit
PF09380 FERM_C, 1 hit
PF00373 FERM_M, 1 hit
PF09379 FERM_N, 1 hit
PF04382 SAB, 1 hit
PRINTSiPR00935 BAND41
PR00661 ERMFAMILY
SMARTiView protein in SMART
SM00295 B41, 1 hit
SM01195 FA, 1 hit
SM01196 FERM_C, 1 hit
SUPFAMiSSF47031 SSF47031, 1 hit
SSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS00660 FERM_1, 1 hit
PS00661 FERM_2, 1 hit
PS50057 FERM_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry namei41_MOUSE
AccessioniPrimary (citable) accession number: P48193
Secondary accession number(s): A2A843
, Q5DTQ8, Q68FF1, Q6NVF5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: May 29, 2007
Last modified: November 7, 2018
This is version 161 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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