UniProtKB - P48182 (ACR2_CAEEL)
Protein
Acetylcholine receptor subunit beta-type acr-2
Gene
acr-2
Organism
Caenorhabditis elegans
Status
Functioni
Non-alpha subunit of nicotinic acetylcholine receptor (nAChR) (PubMed:8581398, PubMed:20027209). Acts in cholinergic motoneurons to regulate presynaptic neurotransmitter release, thereby ensuring normal level of excitation of cholinergic motoneurons during locomotion (PubMed:20027209, PubMed:23658528, PubMed:27782882).3 Publications
GO - Molecular functioni
- acetylcholine-gated cation-selective channel activity Source: WormBase
- excitatory extracellular ligand-gated ion channel activity Source: WormBase
- neurotransmitter receptor activity Source: GO_Central
- transmembrane signaling receptor activity Source: InterPro
GO - Biological processi
- calcium ion import across plasma membrane Source: WormBase
- chemical synaptic transmission Source: GO_Central
- inorganic cation transmembrane transport Source: WormBase
- ion transmembrane transport Source: GO_Central
- nervous system process Source: GO_Central
- neuromuscular synaptic transmission Source: UniProtKB
- regulation of gene expression Source: UniProtKB
- regulation of locomotion Source: UniProtKB
- regulation of membrane potential Source: GO_Central
- regulation of muscle contraction Source: UniProtKB
- signal transduction Source: GO_Central
Keywordsi
Molecular function | Ion channel, Ligand-gated ion channel, Receptor |
Biological process | Ion transport, Transport |
Enzyme and pathway databases
Reactomei | R-CEL-629587, Highly sodium permeable postsynaptic acetylcholine nicotinic receptors R-CEL-629594, Highly calcium permeable postsynaptic nicotinic acetylcholine receptors R-CEL-629597, Highly calcium permeable nicotinic acetylcholine receptors |
Protein family/group databases
TCDBi | 1.A.9.1.13, the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family 1.A.9.1.2, the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family |
Names & Taxonomyi
Protein namesi | Recommended name: Acetylcholine receptor subunit beta-type acr-2 |
Gene namesi | Name:acr-2 ORF Names:K11G12.2 |
Organismi | Caenorhabditis elegans |
Taxonomic identifieri | 6239 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditina › Rhabditomorpha › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Proteomesi |
|
Organism-specific databases
WormBasei | K11G12.2 ; CE07380 ; WBGene00000042 ; acr-2 |
Subcellular locationi
Plasma membrane
- postsynaptic cell membrane By similarity; Multi-pass membrane protein Sequence analysis
- Cell membrane By similarity; Multi-pass membrane protein Sequence analysis
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
- postsynaptic membrane Source: UniProtKB-SubCell
Other locations
- neuron projection Source: WormBase
- synapse Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 21 – 270 | ExtracellularSequence analysisAdd BLAST | 250 | |
Transmembranei | 271 – 291 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 299 – 319 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 331 – 351 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 352 – 527 | CytoplasmicSequence analysisAdd BLAST | 176 | |
Transmembranei | 528 – 548 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, SynapsePathology & Biotechi
Disruption phenotypei
Locomotion is slower. Moderately resistant to paralysis induced by acetylcholine esterase inhibitor aldicarb but sensitivity to acetylcholine agonist levamisole is normal. Reduced both cholinergic and GABAergic motoneuron activities characterized by a reduction in miniature postsynaptic currents in muscles.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 309 | V → M in n2420; constitutively active. Spontaneous convulsions and shrinking behavior in response to touch. Convulsions are suppressed in an unc-74, unc-50, unc-38 or unc-63 mutant background. Enhanced neurotransmitter release by cholinergic motoneurons and reduced neurotransmitter release by GABAergic motoneurons. Increases neuropeptide flp-18 expression in cholinergic B-type motor neurons. Increases sensitivity to paralysis induced by acetylcholine esterase inhibitor aldicarb or acetylcholine agonist levamisole in L3-L4 larvae and adults. 2 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | Sequence analysisAdd BLAST | 20 | |
ChainiPRO_0000000398 | 21 – 575 | Acetylcholine receptor subunit beta-type acr-2Add BLAST | 555 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 41 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 42 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 80 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 131 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 185 ↔ 199 | By similarity |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | P48182 |
Expressioni
Tissue specificityi
Specifically expressed in cholinergic ventral cord motoneurons of the VA, VB, DA and DB classes but not AS and VC classes. Expressed in PVQ and DVC neurons in the tail.1 Publication
Developmental stagei
Expressed from L1 larval stage through adults.1 Publication
Gene expression databases
Bgeei | WBGene00000042, Expressed in multi-cellular organism and 4 other tissues |
Interactioni
Subunit structurei
Component of nicotinic acetylcholine receptor. In cholinergic motoneurons, composed of 2 non-alpha subunits acr-2 and acr-3, and 3 alpha subunits unc-38, unc-63 and acr-12.
2 PublicationsProtein-protein interaction databases
STRINGi | 6239.K11G12.2 |
Family & Domainsi
Sequence similaritiesi
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. [View classification]Curated
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3645, Eukaryota |
HOGENOMi | CLU_018074_1_0_1 |
InParanoidi | P48182 |
OMAi | SHKMPTW |
OrthoDBi | 381858at2759 |
PhylomeDBi | P48182 |
Family and domain databases
Gene3Di | 2.70.170.10, 1 hit |
InterProi | View protein in InterPro IPR006202, Neur_chan_lig-bd IPR036734, Neur_chan_lig-bd_sf IPR006201, Neur_channel IPR036719, Neuro-gated_channel_TM_sf IPR006029, Neurotrans-gated_channel_TM IPR018000, Neurotransmitter_ion_chnl_CS IPR002394, Nicotinic_acetylcholine_rcpt |
PANTHERi | PTHR18945, PTHR18945, 1 hit |
Pfami | View protein in Pfam PF02931, Neur_chan_LBD, 1 hit PF02932, Neur_chan_memb, 1 hit |
PRINTSi | PR00254, NICOTINICR PR00252, NRIONCHANNEL |
SUPFAMi | SSF63712, SSF63712, 1 hit SSF90112, SSF90112, 1 hit |
TIGRFAMsi | TIGR00860, LIC, 1 hit |
PROSITEi | View protein in PROSITE PS00236, NEUROTR_ION_CHANNEL, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P48182-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKKTVKILLI LITVFLKVHC NGGHDDEAAD FLSHTNIDDP NNSSDPNKNS
60 70 80 90 100
DQGDTMGEDE DRLVIDLFRE YNFLIRPVKN VSSPPVVVDF GVAMILLINV
110 120 130 140 150
DEKNQILQTN VWLTMKWNDF QLAWNPAEYG NISNLHVPSD RVWLPDIVLF
160 170 180 190 200
NNADGNYEVS FKSNVFVDHH GDVTWVPPAM FKSSCRIDVE WFPFDEQCCT
210 220 230 240 250
LVFGSWTYNS EEVRLHWYNN IQAVQLHDYS YSGIWDVIDV PGQLVHKPDL
260 270 280 290 300
KENKMVFNVV IRRKTLFYTV ILIIPTVLMA FLSVMAFYLP VDSGEKVSLT
310 320 330 340 350
ISLLLALVVF LLLVSKILPP TSNIPLMGKY LLLAFVLNIT AVVGTVVIVN
360 370 380 390 400
IYFRSALSHK MPTWVRKVFL EFLPHLLVMK RPERIPIFNG YFVEEYCASE
410 420 430 440 450
IFDASLVMPS MTATMLPFLQ VTTNLKAASS TSSGQSSEHH ENCSKWKKRL
460 470 480 490 500
SIRMSKRRAP RARLDDDSED IIDDTNGNHV DSLQEKISKE MKTTVEAIAY
510 520 530 540 550
IAEHMKREMS LKKMRDDWKY VAMVLDRLIL LIFFGVTLGG TLGIICSAPH
560 570
VFDFVDQEAI ISKLNAKYLP SDMYS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X86403 mRNA Translation: CAA60157.1 FO081325 Genomic DNA Translation: CCD70794.1 |
PIRi | S57648 |
RefSeqi | NP_509128.1, NM_076727.5 |
Genome annotation databases
EnsemblMetazoai | K11G12.2.1; K11G12.2.1; WBGene00000042 |
GeneIDi | 180936 |
KEGGi | cel:CELE_K11G12.2 |
UCSCi | K11G12.2, c. elegans |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X86403 mRNA Translation: CAA60157.1 FO081325 Genomic DNA Translation: CCD70794.1 |
PIRi | S57648 |
RefSeqi | NP_509128.1, NM_076727.5 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 6239.K11G12.2 |
Protein family/group databases
TCDBi | 1.A.9.1.13, the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family 1.A.9.1.2, the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family |
Proteomic databases
PaxDbi | P48182 |
Genome annotation databases
EnsemblMetazoai | K11G12.2.1; K11G12.2.1; WBGene00000042 |
GeneIDi | 180936 |
KEGGi | cel:CELE_K11G12.2 |
UCSCi | K11G12.2, c. elegans |
Organism-specific databases
CTDi | 180936 |
WormBasei | K11G12.2 ; CE07380 ; WBGene00000042 ; acr-2 |
Phylogenomic databases
eggNOGi | KOG3645, Eukaryota |
HOGENOMi | CLU_018074_1_0_1 |
InParanoidi | P48182 |
OMAi | SHKMPTW |
OrthoDBi | 381858at2759 |
PhylomeDBi | P48182 |
Enzyme and pathway databases
Reactomei | R-CEL-629587, Highly sodium permeable postsynaptic acetylcholine nicotinic receptors R-CEL-629594, Highly calcium permeable postsynaptic nicotinic acetylcholine receptors R-CEL-629597, Highly calcium permeable nicotinic acetylcholine receptors |
Miscellaneous databases
PROi | PR:P48182 |
Gene expression databases
Bgeei | WBGene00000042, Expressed in multi-cellular organism and 4 other tissues |
Family and domain databases
Gene3Di | 2.70.170.10, 1 hit |
InterProi | View protein in InterPro IPR006202, Neur_chan_lig-bd IPR036734, Neur_chan_lig-bd_sf IPR006201, Neur_channel IPR036719, Neuro-gated_channel_TM_sf IPR006029, Neurotrans-gated_channel_TM IPR018000, Neurotransmitter_ion_chnl_CS IPR002394, Nicotinic_acetylcholine_rcpt |
PANTHERi | PTHR18945, PTHR18945, 1 hit |
Pfami | View protein in Pfam PF02931, Neur_chan_LBD, 1 hit PF02932, Neur_chan_memb, 1 hit |
PRINTSi | PR00254, NICOTINICR PR00252, NRIONCHANNEL |
SUPFAMi | SSF63712, SSF63712, 1 hit SSF90112, SSF90112, 1 hit |
TIGRFAMsi | TIGR00860, LIC, 1 hit |
PROSITEi | View protein in PROSITE PS00236, NEUROTR_ION_CHANNEL, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ACR2_CAEEL | |
Accessioni | P48182Primary (citable) accession number: P48182 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1996 |
Last sequence update: | February 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 154 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Caenorhabditis annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Caenorhabditis elegans
Caenorhabditis elegans: entries, gene names and cross-references to WormBase - SIMILARITY comments
Index of protein domains and families