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Protein

Glycine receptor subunit beta

Gene

Glrb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Glycine receptors are ligand-gated chloride channels. GLRB does not form ligand-gated ion channels by itself, but is part of heteromeric ligand-gated chloride channels. Channel opening is triggered by extracellular glycine (PubMed:12809985). Heteropentameric channels composed of GLRB and GLRA1 are activated by lower glycine levels than homopentameric GLRA1. Plays an important role in the down-regulation of neuronal excitability. Contributes to the generation of inhibitory postsynaptic currents (PubMed:12809985, PubMed:16672662).By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei307Important for obstruction of the ion pore in the closed conformationBy similarity1

GO - Molecular functioni

  • drug binding Source: MGI
  • extracellularly glycine-gated chloride channel activity Source: MGI
  • extracellularly glycine-gated ion channel activity Source: UniProtKB
  • glycine binding Source: MGI
  • transmembrane signaling receptor activity Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionChloride channel, Ion channel, Ligand-gated ion channel, Receptor
Biological processIon transport, Transport
LigandChloride

Enzyme and pathway databases

ReactomeiR-MMU-112314 Neurotransmitter receptors and postsynaptic signal transmission

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine receptor subunit beta
Alternative name(s):
Glycine receptor 58 kDa subunit
Gene namesi
Name:Glrb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:95751 Glrb

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 268ExtracellularBy similarityAdd BLAST246
Transmembranei269 – 290Helical; Name=1By similarityAdd BLAST22
Topological domaini291 – 295CytoplasmicBy similarity5
Transmembranei296 – 316Helical; Name=2By similarityAdd BLAST21
Topological domaini317 – 327ExtracellularBy similarityAdd BLAST11
Transmembranei328 – 348Helical; Name=3By similarityAdd BLAST21
Topological domaini349 – 474CytoplasmicBy similarityAdd BLAST126
Transmembranei475 – 495Helical; Name=4By similarityAdd BLAST21

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Involvement in diseasei

Defects in Glrb cause the spastic condition which is characterized by muscle rigidity, tremors, myoclonic jerks, pronounced startle reaction, abnormal gait and impaired righting ability (PubMed:7920630). Neurons from the ventral horn of the spinal cord display reduced inhibitory postsynaptic currents (PubMed:12809985). Likewise, hypoglossal neurons display a dramatic reduction in the frequency and amplitude of postsynaptic inhibitory currents (PubMed:16672662).3 Publications

Keywords - Diseasei

Disease mutation

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22By similarityAdd BLAST22
ChainiPRO_000000042423 – 496Glycine receptor subunit betaAdd BLAST474

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi54N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi183 ↔ 197By similarity
Glycosylationi242N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi243 ↔ 255By similarity
Modified residuei391PhosphothreonineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP48168
PRIDEiP48168

PTM databases

iPTMnetiP48168
PhosphoSitePlusiP48168

Expressioni

Tissue specificityi

Detected in spinal cord, brain and brain stem, especially in the periolivary region, spinal nuclei, trigeminal nucleus, medulla oblongata, pons and midbrain. Detected in the inner plexiform layer of the retina (at protein level) (PubMed:22592841). High levels of expression in cortex, hippocampus, thalamus and cerebellum (PubMed:7920630). Detected in spinal cord (PubMed:7946325).3 Publications

Gene expression databases

BgeeiENSMUSG00000028020 Expressed in 251 organ(s), highest expression level in spinal cord
CleanExiMM_GLRB
ExpressionAtlasiP48168 baseline and differential
GenevisibleiP48168 MM

Interactioni

Subunit structurei

Heteropentamer composed of GLRB and GLRA1. Heteropentamer composed of GLRB and GLRA2. Heteropentamer composed of GLRB and GLRA3. Heteropentamer composed of two GLRA1 and three GLRB subunits. Heteropentamer composed of three GLRA1 and two GLRB subunits. Interacts with GPHN.By similarity

Binary interactionsi

Protein-protein interaction databases

IntActiP48168, 5 interactors
MINTiP48168
STRINGi10090.ENSMUSP00000029654

Structurei

3D structure databases

ProteinModelPortaliP48168
SMRiP48168
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni247 – 253Strychnine-bindingBy similarity7

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3644 Eukaryota
ENOG410XP43 LUCA
GeneTreeiENSGT00760000118821
HOGENOMiHOG000231336
HOVERGENiHBG051707
InParanoidiP48168
KOiK05196
OMAiPRLKLPN
OrthoDBiEOG091G0805
TreeFamiTF315453

Family and domain databases

Gene3Di2.70.170.10, 1 hit
InterProiView protein in InterPro
IPR008060 Glycine_rcpt_B
IPR006202 Neur_chan_lig-bd
IPR036734 Neur_chan_lig-bd_sf
IPR006201 Neur_channel
IPR036719 Neuro-gated_channel_TM_sf
IPR006029 Neurotrans-gated_channel_TM
IPR018000 Neurotransmitter_ion_chnl_CS
PANTHERiPTHR18945 PTHR18945, 1 hit
PTHR18945:SF29 PTHR18945:SF29, 1 hit
PfamiView protein in Pfam
PF02931 Neur_chan_LBD, 1 hit
PF02932 Neur_chan_memb, 1 hit
PRINTSiPR01677 GLYRBETA
PR00252 NRIONCHANNEL
SUPFAMiSSF63712 SSF63712, 1 hit
SSF90112 SSF90112, 1 hit
TIGRFAMsiTIGR00860 LIC, 1 hit
PROSITEiView protein in PROSITE
PS00236 NEUROTR_ION_CHANNEL, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

P48168-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKFSLAISFF ILMSLLFEDA CAKEKSSKKG KGKKKQYLCP SQQSPEDLAR
60 70 80 90 100
VPPNSTSNIL NRLLVSYDPR IRPNFKGIPV DVVVNIFINS FGSIQETTMD
110 120 130 140 150
YRVNIFLRQK WNDPRLKLPS DFRGSDALTV DPTMYKCLWK PDLFFANEKS
160 170 180 190 200
ANFHDVTQEN ILLFIFRDGD VLVSMRLSIT LSCPLDLTLF PMDTQRCKMQ
210 220 230 240 250
LESFGYTTDD LRFIWQSGDP VQLEKIALPQ FDIKKEDIEY GNCTKYYKGT
260 270 280 290 300
GYYTCVEVIF TLRRQVGFYM MGVYAPTLLI VVLSWLSFWI NPDASAARVP
310 320 330 340 350
LGIFSVLSLA SECTTLAAEL PKVSYVKALD VWLIACLLFG FASLVEYAVV
360 370 380 390 400
QVMLNNPKRV EAEKARIAKA EQADGKGGNA AKKNTVNGTG TPVHISTLQV
410 420 430 440 450
GETRCKKVCT SKSDLRSNDF SIVGSLPRDF ELSNYDCYGK PIEVNNGLGK
460 470 480 490
PQAKNKKPPP AKPVIPTAAK RIDLYARALF PFCFLFFNVI YWSIYL
Length:496
Mass (Da):55,951
Last modified:July 27, 2011 - v2
Checksum:i554840A6DE9DE7BE
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A1KR23A1KR23_MOUSE
Glycine receptor beta subunit
Glrb
445Annotation score:
E9Q3E3E9Q3E3_MOUSE
Glycine receptor subunit beta
Glrb
307Annotation score:
E9Q9K1E9Q9K1_MOUSE
Glycine receptor subunit beta
Glrb
58Annotation score:
E9PV81E9PV81_MOUSE
Glycine receptor subunit beta
Glrb
57Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti365A → R in AAA61874 (PubMed:7920630).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti74 – 83NFKGIPVDVV → TTMLDIQPMI in spastic 1. 1 Publication10
Natural varianti84 – 496Missing in spastic 1. 1 PublicationAdd BLAST413
Natural varianti143 – 151LFFANEKSA → VSMSWIYNR in spastic 2. 1 Publication9
Natural varianti152 – 496Missing in spastic 2. 1 PublicationAdd BLAST345

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09399 mRNA Translation: AAA61874.1
X81202 mRNA Translation: CAA57076.1
X81201 Genomic DNA Translation: CAA57075.1
L32594 Genomic DNA Translation: AAA65966.1
AK083251 mRNA Translation: BAC38831.1
CH466547 Genomic DNA Translation: EDL15445.1
BC037605 mRNA Translation: AAH37605.1
CCDSiCCDS17424.1
PIRiS46459
RefSeqiNP_001268898.1, NM_001281969.2
NP_001332884.1, NM_001345955.1
NP_001332885.1, NM_001345956.1
NP_001332886.1, NM_001345957.1
NP_001332887.1, NM_001345958.1
NP_001332888.1, NM_001345959.1
NP_001332925.1, NM_001345996.1
NP_034428.2, NM_010298.6
UniGeneiMm.275639

Genome annotation databases

EnsembliENSMUST00000029654; ENSMUSP00000029654; ENSMUSG00000028020
GeneIDi14658
KEGGimmu:14658
UCSCiuc008pog.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09399 mRNA Translation: AAA61874.1
X81202 mRNA Translation: CAA57076.1
X81201 Genomic DNA Translation: CAA57075.1
L32594 Genomic DNA Translation: AAA65966.1
AK083251 mRNA Translation: BAC38831.1
CH466547 Genomic DNA Translation: EDL15445.1
BC037605 mRNA Translation: AAH37605.1
CCDSiCCDS17424.1
PIRiS46459
RefSeqiNP_001268898.1, NM_001281969.2
NP_001332884.1, NM_001345955.1
NP_001332885.1, NM_001345956.1
NP_001332886.1, NM_001345957.1
NP_001332887.1, NM_001345958.1
NP_001332888.1, NM_001345959.1
NP_001332925.1, NM_001345996.1
NP_034428.2, NM_010298.6
UniGeneiMm.275639

3D structure databases

ProteinModelPortaliP48168
SMRiP48168
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP48168, 5 interactors
MINTiP48168
STRINGi10090.ENSMUSP00000029654

PTM databases

iPTMnetiP48168
PhosphoSitePlusiP48168

Proteomic databases

PaxDbiP48168
PRIDEiP48168

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029654; ENSMUSP00000029654; ENSMUSG00000028020
GeneIDi14658
KEGGimmu:14658
UCSCiuc008pog.2 mouse

Organism-specific databases

CTDi2743
MGIiMGI:95751 Glrb

Phylogenomic databases

eggNOGiKOG3644 Eukaryota
ENOG410XP43 LUCA
GeneTreeiENSGT00760000118821
HOGENOMiHOG000231336
HOVERGENiHBG051707
InParanoidiP48168
KOiK05196
OMAiPRLKLPN
OrthoDBiEOG091G0805
TreeFamiTF315453

Enzyme and pathway databases

ReactomeiR-MMU-112314 Neurotransmitter receptors and postsynaptic signal transmission

Miscellaneous databases

PROiPR:P48168
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028020 Expressed in 251 organ(s), highest expression level in spinal cord
CleanExiMM_GLRB
ExpressionAtlasiP48168 baseline and differential
GenevisibleiP48168 MM

Family and domain databases

Gene3Di2.70.170.10, 1 hit
InterProiView protein in InterPro
IPR008060 Glycine_rcpt_B
IPR006202 Neur_chan_lig-bd
IPR036734 Neur_chan_lig-bd_sf
IPR006201 Neur_channel
IPR036719 Neuro-gated_channel_TM_sf
IPR006029 Neurotrans-gated_channel_TM
IPR018000 Neurotransmitter_ion_chnl_CS
PANTHERiPTHR18945 PTHR18945, 1 hit
PTHR18945:SF29 PTHR18945:SF29, 1 hit
PfamiView protein in Pfam
PF02931 Neur_chan_LBD, 1 hit
PF02932 Neur_chan_memb, 1 hit
PRINTSiPR01677 GLYRBETA
PR00252 NRIONCHANNEL
SUPFAMiSSF63712 SSF63712, 1 hit
SSF90112 SSF90112, 1 hit
TIGRFAMsiTIGR00860 LIC, 1 hit
PROSITEiView protein in PROSITE
PS00236 NEUROTR_ION_CHANNEL, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGLRB_MOUSE
AccessioniPrimary (citable) accession number: P48168
Secondary accession number(s): Q5U5X3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: July 27, 2011
Last modified: October 10, 2018
This is version 154 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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