UniProtKB - P48148 (RHO1_DROME)
Protein
Ras-like GTP-binding protein Rho1
Gene
Rho1
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Has a role in regulating actin cytoskeletal organization: required during early development for proper execution of morphogenetic movements of individual cells and groups of cells important for the formation of the embryonic body plan (PubMed:10556060, PubMed:24535826, PubMed:25739458). Plays a role in regulating dorsal closure during embryogenesis (PubMed:10556060, PubMed:10323867). During axis elongation, required for Rho-kinase Rok planar polarity and adherens junction localization as well as for generating a planar polarized distribution of the actin-binding protein Shrm (PubMed:24535826). During embryogenesis, acts upstream of wash to regulate the developmental migration of tail hemocytes anteriorly along the ventral midline (PubMed:25739458). May have a role in eye development (PubMed:7835340).5 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 12 – 19 | GTPBy similarity | 8 | |
Nucleotide bindingi | 59 – 63 | GTPBy similarity | 5 | |
Nucleotide bindingi | 117 – 120 | GTPBy similarity | 4 |
GO - Molecular functioni
- actin binding Source: UniProtKB-KW
- GTPase activity Source: FlyBase
- GTP binding Source: GO_Central
- kinase binding Source: FlyBase
- protein kinase binding Source: GO_Central
GO - Biological processi
- actin cytoskeleton organization Source: FlyBase
- actin cytoskeleton reorganization Source: FlyBase
- actin filament bundle assembly Source: FlyBase
- actin filament organization Source: FlyBase
- actin-mediated cell contraction Source: FlyBase
- adherens junction maintenance Source: FlyBase
- axon guidance Source: FlyBase
- border follicle cell migration Source: FlyBase
- cell elongation involved in imaginal disc-derived wing morphogenesis Source: FlyBase
- cell migration Source: GO_Central
- cellularization Source: FlyBase
- cellular protein localization Source: FlyBase
- compound eye morphogenesis Source: FlyBase
- cortical actin cytoskeleton organization Source: FlyBase
- cortical cytoskeleton organization Source: GO_Central
- dendrite morphogenesis Source: FlyBase
- dendrite self-avoidance Source: FlyBase
- determination of left/right symmetry Source: FlyBase
- dorsal closure Source: UniProtKB
- dorsal closure, leading edge cell differentiation Source: FlyBase
- dorsal closure, spreading of leading edge cells Source: FlyBase
- endocytic recycling Source: FlyBase
- endocytosis Source: FlyBase
- establishment of planar polarity of embryonic epithelium Source: FlyBase
- establishment of protein localization Source: FlyBase
- establishment or maintenance of actin cytoskeleton polarity Source: FlyBase
- establishment or maintenance of cell polarity Source: GO_Central
- gastrulation involving germ band extension Source: FlyBase
- germ-band extension Source: FlyBase
- glial cell migration Source: FlyBase
- hemocyte migration Source: FlyBase
- imaginal disc-derived leg morphogenesis Source: FlyBase
- imaginal disc-derived wing hair organization Source: FlyBase
- lumen formation, open tracheal system Source: FlyBase
- maintenance of epithelial cell apical/basal polarity Source: FlyBase
- melanization defense response Source: FlyBase
- mitotic actomyosin contractile ring assembly Source: FlyBase
- mitotic cytokinesis Source: FlyBase
- motor neuron axon guidance Source: FlyBase
- negative regulation of canonical Wnt signaling pathway Source: FlyBase
- neuroblast proliferation Source: FlyBase
- neuron projection morphogenesis Source: FlyBase
- open tracheal system development Source: FlyBase
- peripheral nervous system development Source: FlyBase
- positive regulation of protein serine/threonine kinase activity Source: UniProtKB
- positive regulation of wound healing Source: FlyBase
- posterior midgut invagination Source: FlyBase
- protein localization involved in establishment of planar polarity Source: FlyBase
- protein localization to adherens junction Source: FlyBase
- pseudocleavage involved in syncytial blastoderm formation Source: FlyBase
- regulation of actin cytoskeleton organization Source: GO_Central
- regulation of actomyosin structure organization Source: FlyBase
- regulation of axonogenesis Source: FlyBase
- regulation of cell migration Source: FlyBase
- regulation of cell shape Source: GO_Central
- regulation of cytoskeleton organization Source: FlyBase
- regulation of embryonic cell shape Source: FlyBase
- regulation of filopodium assembly Source: FlyBase
- regulation of locomotor rhythm Source: FlyBase
- regulation of Malpighian tubule size Source: FlyBase
- regulation of myoblast fusion Source: FlyBase
- regulation of tube length, open tracheal system Source: FlyBase
- response to wounding Source: FlyBase
- salivary gland morphogenesis Source: FlyBase
- small GTPase mediated signal transduction Source: InterPro
- spiracle morphogenesis, open tracheal system Source: FlyBase
- tracheal pit formation in open tracheal system Source: FlyBase
- ventral furrow formation Source: FlyBase
- visual perception Source: UniProtKB-KW
- wound healing Source: FlyBase
Keywordsi
Molecular function | Actin-binding, Developmental protein |
Biological process | Sensory transduction, Vision |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-DME-114604, GPVI-mediated activation cascade R-DME-193634, Axonal growth inhibition (RHOA activation) R-DME-194840, Rho GTPase cycle R-DME-198203, PI3K/AKT activation R-DME-2173791, TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition) R-DME-350368, Activation of RHO1 by FZ:DSH complex R-DME-350407, RHO1 GTPase cycle R-DME-350480, Activation of non-muscle Myosin II R-DME-392451, G beta:gamma signalling through PI3Kgamma R-DME-3928662, EPHB-mediated forward signaling R-DME-3928663, EPHA-mediated growth cone collapse R-DME-416482, G alpha (12/13) signalling events R-DME-416572, Sema4D induced cell migration and growth-cone collapse R-DME-4420097, VEGFA-VEGFR2 Pathway R-DME-450728, Inhibition of actin polymerisation R-DME-5625740, RHO GTPases activate PKNs R-DME-5625900, RHO GTPases activate CIT R-DME-5627117, RHO GTPases Activate ROCKs R-DME-5663220, RHO GTPases Activate Formins R-DME-5666185, RHO GTPases Activate Rhotekin and Rhophilins R-DME-5689896, Ovarian tumor domain proteases R-DME-6785631, ERBB2 Regulates Cell Motility R-DME-6798695, Neutrophil degranulation R-DME-8849471, PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases |
Names & Taxonomyi
Protein namesi | Recommended name: Ras-like GTP-binding protein Rho1 |
Gene namesi | Name:Rho1Imported ORF Names:CG8416Imported |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0014020, Rho1 |
Subcellular locationi
Plasma membrane
- Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated
- Apical cell membrane 1 Publication
- Lateral cell membrane 1 Publication
Cytoskeleton
Cytoskeleton
- cytoskeleton Source: GO_Central
Cytosol
- cytosol Source: Reactome
Plasma Membrane
- apical plasma membrane Source: UniProtKB-SubCell
- lateral plasma membrane Source: UniProtKB-SubCell
- plasma membrane Source: FlyBase
Other locations
- apical cortex Source: FlyBase
- cell cortex Source: FlyBase
- cell hair Source: FlyBase
- cell projection Source: GO_Central
- cell-cell contact zone Source: FlyBase
- cytoplasm Source: FlyBase
- cytoplasmic vesicle Source: GO_Central
- intracellular membrane-bounded organelle Source: GO_Central
- vesicle Source: GO_Central
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Cytoskeleton, MembranePathology & Biotechi
Disruption phenotypei
Embryonic lethal. Embryos exhibit severe defects in head involution and imperfect dorsal closure. During head involution, the head structures fail to internalize resulting in holes in the dorsal anterior region of the cuticle. The disruption in the dorsal surface stretches the ventral surface, causing the cuticle to bow.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 27 – 29 | KDQ → AAA: Reduces binding to wash. Reduces the number of hemocytes migrating anteriorly from the tail during embryogenesis. 1 Publication | 3 | |
Mutagenesisi | 39 | F → V: No effect on binding to wash and no effect on tail hemocyte developmental migration from the tail. 1 Publication | 1 | |
Mutagenesisi | 51 – 54 | KQVE → AAAA: No effect on binding to wash. 1 Publication | 4 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000198885 | 1 – 189 | Ras-like GTP-binding protein Rho1Add BLAST | 189 | |
PropeptideiPRO_0000281238 | 190 – 192 | Removed in mature formBy similarity | 3 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 189 | Cysteine methyl esterBy similarity | 1 | |
Lipidationi | 189 | S-geranylgeranyl cysteineBy similarity | 1 |
Keywords - PTMi
Lipoprotein, Methylation, PrenylationProteomic databases
PaxDbi | P48148 |
PRIDEi | P48148 |
Expressioni
Tissue specificityi
Expressed in hemocytes (at protein level).1 Publication
Gene expression databases
Bgeei | FBgn0014020, Expressed in saliva-secreting gland and 52 other tissues |
Genevisiblei | P48148, DM |
Interactioni
Subunit structurei
GO - Molecular functioni
- actin binding Source: UniProtKB-KW
- kinase binding Source: FlyBase
- protein kinase binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 62500, 91 interactors |
DIPi | DIP-22676N |
IntActi | P48148, 1 interactor |
STRINGi | 7227.FBpp0086354 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 34 – 42 | Effector regionSequence analysis | 9 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0393, Eukaryota |
GeneTreei | ENSGT00950000182945 |
HOGENOMi | CLU_041217_21_2_1 |
InParanoidi | P48148 |
OMAi | RWIPEIK |
OrthoDBi | 1166960at2759 |
PhylomeDBi | P48148 |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase IPR003578, Small_GTPase_Rho |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51420, RHO, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P48148-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTTIRKKLVI VGDGACGKTC LLIVFSKDQF PEVYVPTVFE NYVADIEVDG
60 70 80 90 100
KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSVDSPD SLENIPEKWT
110 120 130 140 150
PEVKHFCPNV PIILVGNKKD LRNDPNTIRD LAKMKQEPVK PQEGRAMAEK
160 170 180 190
INAFAYLECS AKSKEGVRDV FETATRAALQ VKKRKKTRCL LL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L38311 mRNA Translation: AAA67042.1 AF177871 Genomic DNA Translation: AAF01183.1 AF177872 mRNA Translation: AAF01184.1 AF177873 mRNA Translation: AAF01185.1 AF177874 mRNA Translation: AAF01186.1 AE013599 Genomic DNA Translation: AAM70944.1 AE013599 Genomic DNA Translation: AAM70945.1 AE013599 Genomic DNA Translation: AAM70946.1 AE013599 Genomic DNA Translation: AAS64843.1 AE013599 Genomic DNA Translation: AAS64844.1 AY119536 mRNA Translation: AAM50190.1 BT010085 mRNA Translation: AAQ22554.1 |
PIRi | S54294 |
RefSeqi | NP_477098.1, NM_057750.4 NP_599135.1, NM_134308.3 NP_599136.1, NM_134309.2 NP_725524.1, NM_166139.3 NP_995849.1, NM_206127.2 NP_995850.1, NM_206128.2 |
Genome annotation databases
EnsemblMetazoai | FBtr0087211; FBpp0086353; FBgn0014020 FBtr0087212; FBpp0086354; FBgn0014020 FBtr0087213; FBpp0086355; FBgn0014020 FBtr0087214; FBpp0086356; FBgn0014020 FBtr0087216; FBpp0089129; FBgn0014020 FBtr0087217; FBpp0089130; FBgn0014020 |
GeneIDi | 36775 |
KEGGi | dme:Dmel_CG8416 |
UCSCi | CG8416-RC, d. melanogaster |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L38311 mRNA Translation: AAA67042.1 AF177871 Genomic DNA Translation: AAF01183.1 AF177872 mRNA Translation: AAF01184.1 AF177873 mRNA Translation: AAF01185.1 AF177874 mRNA Translation: AAF01186.1 AE013599 Genomic DNA Translation: AAM70944.1 AE013599 Genomic DNA Translation: AAM70945.1 AE013599 Genomic DNA Translation: AAM70946.1 AE013599 Genomic DNA Translation: AAS64843.1 AE013599 Genomic DNA Translation: AAS64844.1 AY119536 mRNA Translation: AAM50190.1 BT010085 mRNA Translation: AAQ22554.1 |
PIRi | S54294 |
RefSeqi | NP_477098.1, NM_057750.4 NP_599135.1, NM_134308.3 NP_599136.1, NM_134309.2 NP_725524.1, NM_166139.3 NP_995849.1, NM_206127.2 NP_995850.1, NM_206128.2 |
3D structure databases
SMRi | P48148 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 62500, 91 interactors |
DIPi | DIP-22676N |
IntActi | P48148, 1 interactor |
STRINGi | 7227.FBpp0086354 |
Proteomic databases
PaxDbi | P48148 |
PRIDEi | P48148 |
Genome annotation databases
EnsemblMetazoai | FBtr0087211; FBpp0086353; FBgn0014020 FBtr0087212; FBpp0086354; FBgn0014020 FBtr0087213; FBpp0086355; FBgn0014020 FBtr0087214; FBpp0086356; FBgn0014020 FBtr0087216; FBpp0089129; FBgn0014020 FBtr0087217; FBpp0089130; FBgn0014020 |
GeneIDi | 36775 |
KEGGi | dme:Dmel_CG8416 |
UCSCi | CG8416-RC, d. melanogaster |
Organism-specific databases
CTDi | 36775 |
FlyBasei | FBgn0014020, Rho1 |
Phylogenomic databases
eggNOGi | KOG0393, Eukaryota |
GeneTreei | ENSGT00950000182945 |
HOGENOMi | CLU_041217_21_2_1 |
InParanoidi | P48148 |
OMAi | RWIPEIK |
OrthoDBi | 1166960at2759 |
PhylomeDBi | P48148 |
Enzyme and pathway databases
Reactomei | R-DME-114604, GPVI-mediated activation cascade R-DME-193634, Axonal growth inhibition (RHOA activation) R-DME-194840, Rho GTPase cycle R-DME-198203, PI3K/AKT activation R-DME-2173791, TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition) R-DME-350368, Activation of RHO1 by FZ:DSH complex R-DME-350407, RHO1 GTPase cycle R-DME-350480, Activation of non-muscle Myosin II R-DME-392451, G beta:gamma signalling through PI3Kgamma R-DME-3928662, EPHB-mediated forward signaling R-DME-3928663, EPHA-mediated growth cone collapse R-DME-416482, G alpha (12/13) signalling events R-DME-416572, Sema4D induced cell migration and growth-cone collapse R-DME-4420097, VEGFA-VEGFR2 Pathway R-DME-450728, Inhibition of actin polymerisation R-DME-5625740, RHO GTPases activate PKNs R-DME-5625900, RHO GTPases activate CIT R-DME-5627117, RHO GTPases Activate ROCKs R-DME-5663220, RHO GTPases Activate Formins R-DME-5666185, RHO GTPases Activate Rhotekin and Rhophilins R-DME-5689896, Ovarian tumor domain proteases R-DME-6785631, ERBB2 Regulates Cell Motility R-DME-6798695, Neutrophil degranulation R-DME-8849471, PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases |
Miscellaneous databases
BioGRID-ORCSi | 36775, 3 hits in 3 CRISPR screens |
ChiTaRSi | Rho1, fly |
GenomeRNAii | 36775 |
PROi | PR:P48148 |
Gene expression databases
Bgeei | FBgn0014020, Expressed in saliva-secreting gland and 52 other tissues |
Genevisiblei | P48148, DM |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase IPR003578, Small_GTPase_Rho |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51420, RHO, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RHO1_DROME | |
Accessioni | P48148Primary (citable) accession number: P48148 Secondary accession number(s): A4UZI6, Q0E958, Q9V3J0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1996 |
Last sequence update: | February 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 193 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families