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Protein

Prolyl endopeptidase

Gene

PREP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long.

Catalytic activityi

Hydrolysis of Pro-|-Xaa >> Ala-|-Xaa in oligopeptides.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei554Charge relay systemPROSITE-ProRule annotation1
Active sitei641Charge relay systemPROSITE-ProRule annotation1
Active sitei680Charge relay systemPROSITE-ProRule annotation1

GO - Molecular functioni

  • endopeptidase activity Source: GO_Central
  • oligopeptidase activity Source: GO_Central
  • serine-type endopeptidase activity Source: ProtInc
  • serine-type exopeptidase activity Source: InterPro
  • serine-type peptidase activity Source: ProtInc

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.21.26 2681

Protein family/group databases

ESTHERihuman-PREP S9N_PPCE_Peptidase_S9
MEROPSiS09.001

Names & Taxonomyi

Protein namesi
Recommended name:
Prolyl endopeptidase (EC:3.4.21.26)
Short name:
PE
Alternative name(s):
Post-proline cleaving enzyme
Gene namesi
Name:PREP
Synonyms:PEP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000085377.13
HGNCiHGNC:9358 PREP
MIMi600400 gene
neXtProtiNX_P48147

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi5550
OpenTargetsiENSG00000085377
PharmGKBiPA33730

Chemistry databases

ChEMBLiCHEMBL3202
DrugBankiDB01684 1-Hydroxy-1-Thio-Glycerol
DB08738 1-{3-oxo-3-[(2S)-2-(pyrrolidin-1-ylcarbonyl)pyrrolidin-1-yl]propyl}-3-phenylquinoxalin-2(1H)-one
DB03864 Monothioglycerol
DB00107 Oxytocin
DB03382 S-Oxy Cysteine
DB03535 Z-Pro-Prolinal
GuidetoPHARMACOLOGYi2395

Polymorphism and mutation databases

BioMutaiPREP
DMDMi215273868

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001224011 – 710Prolyl endopeptidaseAdd BLAST710

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei157N6-acetyllysineCombined sources1

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP48147
MaxQBiP48147
PaxDbiP48147
PeptideAtlasiP48147
PRIDEiP48147
ProteomicsDBi55866

PTM databases

iPTMnetiP48147
PhosphoSitePlusiP48147
SwissPalmiP48147

Expressioni

Gene expression databases

BgeeiENSG00000085377 Expressed in 218 organ(s), highest expression level in secondary oocyte
CleanExiHS_PREP
ExpressionAtlasiP48147 baseline and differential
GenevisibleiP48147 HS

Organism-specific databases

HPAiCAB025414
HPA031388
HPA031389
HPA031390

Interactioni

Subunit structurei

Monomer.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GAPDHP044065EBI-1049962,EBI-354056

Protein-protein interaction databases

BioGridi111541, 35 interactors
IntActiP48147, 2 interactors
STRINGi9606.ENSP00000358106

Chemistry databases

BindingDBiP48147

Structurei

Secondary structure

1710
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP48147
SMRiP48147
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP48147

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S9A family.Curated

Phylogenomic databases

eggNOGiKOG2237 Eukaryota
COG1505 LUCA
GeneTreeiENSGT00530000063426
HOGENOMiHOG000238967
HOVERGENiHBG007251
InParanoidiP48147
KOiK01322
OMAiACMTQRP
OrthoDBiEOG091G023D
PhylomeDBiP48147
TreeFamiTF300655

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR002471 Pept_S9_AS
IPR023302 Pept_S9A_N
IPR001375 Peptidase_S9
IPR002470 Peptidase_S9A
PfamiView protein in Pfam
PF00326 Peptidase_S9, 1 hit
PF02897 Peptidase_S9_N, 1 hit
PRINTSiPR00862 PROLIGOPTASE
SUPFAMiSSF53474 SSF53474, 1 hit
PROSITEiView protein in PROSITE
PS00708 PRO_ENDOPEP_SER, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P48147-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLSLQYPDVY RDETAVQDYH GHKICDPYAW LEDPDSEQTK AFVEAQNKIT
60 70 80 90 100
VPFLEQCPIR GLYKERMTEL YDYPKYSCHF KKGKRYFYFY NTGLQNQRVL
110 120 130 140 150
YVQDSLEGEA RVFLDPNILS DDGTVALRGY AFSEDGEYFA YGLSASGSDW
160 170 180 190 200
VTIKFMKVDG AKELPDVLER VKFSCMAWTH DGKGMFYNSY PQQDGKSDGT
210 220 230 240 250
ETSTNLHQKL YYHVLGTDQS EDILCAEFPD EPKWMGGAEL SDDGRYVLLS
260 270 280 290 300
IREGCDPVNR LWYCDLQQES SGIAGILKWV KLIDNFEGEY DYVTNEGTVF
310 320 330 340 350
TFKTNRQSPN YRVINIDFRD PEESKWKVLV PEHEKDVLEW IACVRSNFLV
360 370 380 390 400
LCYLHDVKNI LQLHDLTTGA LLKTFPLDVG SIVGYSGQKK DTEIFYQFTS
410 420 430 440 450
FLSPGIIYHC DLTKEELEPR VFREVTVKGI DASDYQTVQI FYPSKDGTKI
460 470 480 490 500
PMFIVHKKGI KLDGSHPAFL YGYGGFNISI TPNYSVSRLI FVRHMGGILA
510 520 530 540 550
VANIRGGGEY GETWHKGGIL ANKQNCFDDF QCAAEYLIKE GYTSPKRLTI
560 570 580 590 600
NGGSNGGLLV AACANQRPDL FGCVIAQVGV MDMLKFHKYT IGHAWTTDYG
610 620 630 640 650
CSDSKQHFEW LVKYSPLHNV KLPEADDIQY PSMLLLTADH DDRVVPLHSL
660 670 680 690 700
KFIATLQYIV GRSRKQSNPL LIHVDTKAGH GAGKPTAKVI EEVSDMFAFI
710
ARCLNVDWIP
Length:710
Mass (Da):80,700
Last modified:November 25, 2008 - v2
Checksum:i139072B990820B90
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0Y5Y0H0Y5Y0_HUMAN
Prolyl endopeptidase
PREP
45Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4L → F in CAA52605 (PubMed:7959018).Curated1
Sequence conflicti4L → F in BAA04661 (PubMed:8089089).Curated1
Sequence conflicti16V → I in BAA04661 (PubMed:8089089).Curated1
Sequence conflicti245R → C in BAA04661 (PubMed:8089089).Curated1
Sequence conflicti298T → A in BAA04661 (PubMed:8089089).Curated1
Sequence conflicti319R → W in CAA52605 (PubMed:7959018).Curated1
Sequence conflicti440I → L in BAA04661 (PubMed:8089089).Curated1
Sequence conflicti459G → S in BAA04661 (PubMed:8089089).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_047790351L → V. Corresponds to variant dbSNP:rs12192054Ensembl.1
Natural variantiVAR_047791706V → I1 PublicationCorresponds to variant dbSNP:rs1051484Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74496 mRNA Translation: CAA52605.1
D21102 mRNA Translation: BAA04661.1
AY660966 mRNA Translation: AAV70495.1
AL590871 Genomic DNA No translation available.
AL139191 Genomic DNA No translation available.
AL133406 Genomic DNA No translation available.
CH471051 Genomic DNA Translation: EAW48426.1
BC030636 mRNA Translation: AAH30636.1
CCDSiCCDS5053.1
PIRiI38134
JC2257
RefSeqiNP_002717.3, NM_002726.4
UniGeneiHs.436564

Genome annotation databases

EnsembliENST00000369110; ENSP00000358106; ENSG00000085377
GeneIDi5550
KEGGihsa:5550
UCSCiuc003prc.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74496 mRNA Translation: CAA52605.1
D21102 mRNA Translation: BAA04661.1
AY660966 mRNA Translation: AAV70495.1
AL590871 Genomic DNA No translation available.
AL139191 Genomic DNA No translation available.
AL133406 Genomic DNA No translation available.
CH471051 Genomic DNA Translation: EAW48426.1
BC030636 mRNA Translation: AAH30636.1
CCDSiCCDS5053.1
PIRiI38134
JC2257
RefSeqiNP_002717.3, NM_002726.4
UniGeneiHs.436564

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DDUX-ray1.56A2-710[»]
ProteinModelPortaliP48147
SMRiP48147
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111541, 35 interactors
IntActiP48147, 2 interactors
STRINGi9606.ENSP00000358106

Chemistry databases

BindingDBiP48147
ChEMBLiCHEMBL3202
DrugBankiDB01684 1-Hydroxy-1-Thio-Glycerol
DB08738 1-{3-oxo-3-[(2S)-2-(pyrrolidin-1-ylcarbonyl)pyrrolidin-1-yl]propyl}-3-phenylquinoxalin-2(1H)-one
DB03864 Monothioglycerol
DB00107 Oxytocin
DB03382 S-Oxy Cysteine
DB03535 Z-Pro-Prolinal
GuidetoPHARMACOLOGYi2395

Protein family/group databases

ESTHERihuman-PREP S9N_PPCE_Peptidase_S9
MEROPSiS09.001

PTM databases

iPTMnetiP48147
PhosphoSitePlusiP48147
SwissPalmiP48147

Polymorphism and mutation databases

BioMutaiPREP
DMDMi215273868

Proteomic databases

EPDiP48147
MaxQBiP48147
PaxDbiP48147
PeptideAtlasiP48147
PRIDEiP48147
ProteomicsDBi55866

Protocols and materials databases

DNASUi5550
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000369110; ENSP00000358106; ENSG00000085377
GeneIDi5550
KEGGihsa:5550
UCSCiuc003prc.4 human

Organism-specific databases

CTDi5550
DisGeNETi5550
EuPathDBiHostDB:ENSG00000085377.13
GeneCardsiPREP
HGNCiHGNC:9358 PREP
HPAiCAB025414
HPA031388
HPA031389
HPA031390
MIMi600400 gene
neXtProtiNX_P48147
OpenTargetsiENSG00000085377
PharmGKBiPA33730
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2237 Eukaryota
COG1505 LUCA
GeneTreeiENSGT00530000063426
HOGENOMiHOG000238967
HOVERGENiHBG007251
InParanoidiP48147
KOiK01322
OMAiACMTQRP
OrthoDBiEOG091G023D
PhylomeDBiP48147
TreeFamiTF300655

Enzyme and pathway databases

BRENDAi3.4.21.26 2681

Miscellaneous databases

EvolutionaryTraceiP48147
GeneWikiiProlyl_endopeptidase
GenomeRNAii5550
PROiPR:P48147
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000085377 Expressed in 218 organ(s), highest expression level in secondary oocyte
CleanExiHS_PREP
ExpressionAtlasiP48147 baseline and differential
GenevisibleiP48147 HS

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR002471 Pept_S9_AS
IPR023302 Pept_S9A_N
IPR001375 Peptidase_S9
IPR002470 Peptidase_S9A
PfamiView protein in Pfam
PF00326 Peptidase_S9, 1 hit
PF02897 Peptidase_S9_N, 1 hit
PRINTSiPR00862 PROLIGOPTASE
SUPFAMiSSF53474 SSF53474, 1 hit
PROSITEiView protein in PROSITE
PS00708 PRO_ENDOPEP_SER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPPCE_HUMAN
AccessioniPrimary (citable) accession number: P48147
Secondary accession number(s): Q8N6D4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: November 25, 2008
Last modified: November 7, 2018
This is version 173 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
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