UniProtKB - P47983 (ZP2_CANLF)
Zona pellucida sperm-binding protein 2
ZP2
Functioni
Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor.
By similarityGO - Molecular functioni
- structural constituent of egg coat Source: UniProtKB
GO - Biological processi
- binding of sperm to zona pellucida Source: UniProtKB
- humoral immune response mediated by circulating immunoglobulin Source: UniProtKB
- prevention of polyspermy Source: UniProtKB
Keywordsi
Molecular function | Receptor |
Biological process | Fertilization |
Names & Taxonomyi
Protein namesi | Recommended name: Zona pellucida sperm-binding protein 2Alternative name(s): Zona pellucida glycoprotein 2 Short name: Zp-2 Zona pellucida protein A Cleaved into the following chain: |
Gene namesi | Name:ZP2 Synonyms:ZPA |
Organismi | Canis lupus familiaris (Dog) (Canis familiaris) |
Taxonomic identifieri | 9615 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis › |
Proteomesi |
|
Subcellular locationi
Extracellular region or secreted
- Zona pellucida By similarity
Plasma membrane
- Cell membrane By similarity; Single-pass type I membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
Endosome
- multivesicular body Source: UniProtKB
Extracellular region or secreted
- extracellular region Source: UniProtKB-KW
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- collagen-containing extracellular matrix Source: UniProtKB
- egg coat Source: UniProtKB
- inclusion body Source: UniProtKB
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 39 – 684 | ExtracellularSequence analysisAdd BLAST | 646 | |
Transmembranei | 685 – 705 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 706 – 715 | CytoplasmicSequence analysis | 10 |
Keywords - Cellular componenti
Cell membrane, Extracellular matrix, Membrane, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 38 | By similarityAdd BLAST | 38 | |
ChainiPRO_0000041685 | 39 – 637 | Zona pellucida sperm-binding protein 2Add BLAST | 599 | |
ChainiPRO_0000304557 | 39 – ? | Processed zona pellucida sperm-binding protein 2 | ||
PropeptideiPRO_0000041686 | 638 – 715 | Removed in mature formBy similarityAdd BLAST | 78 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 55 ↔ 138 | By similarity | ||
Glycosylationi | 87 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Disulfide bondi | 88 ↔ 106 | By similarity | ||
Glycosylationi | 193 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Glycosylationi | 220 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Glycosylationi | 266 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Glycosylationi | 321 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Disulfide bondi | 369 ↔ 462 | By similarity | ||
Disulfide bondi | 400 ↔ 421 | By similarity | ||
Glycosylationi | 459 | O-linked (GalNAc...) threonineBy similarity | 1 | |
Disulfide bondi | 542 ↔ 612 | By similarity | ||
Disulfide bondi | 563 ↔ 631 | By similarity | ||
Disulfide bondi | 617 ↔ 627 | By similarity |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 171 – 172 | Cleavage; by ASTLBy similarity | 2 | |
Sitei | 637 – 638 | CleavageBy similarity | 2 |
Keywords - PTMi
Cleavage on pair of basic residues, Disulfide bond, GlycoproteinProteomic databases
PaxDbi | P47983 |
Interactioni
Subunit structurei
Can form homopolymers that assemble into long fibers (in vitro). Polymers of ZP2 and ZP3 organized into long filaments cross-linked by ZP1 homodimers.
Interacts with ZP3.
By similarityProtein-protein interaction databases
STRINGi | 9615.ENSCAFP00000026286 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 368 – 634 | ZPPROSITE-ProRule annotationAdd BLAST | 267 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 466 – 488 | DisorderedBy similarityAdd BLAST | 23 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QPI2, Eukaryota |
InParanoidi | P47983 |
OrthoDBi | 586615at2759 |
Family and domain databases
Gene3Di | 2.60.40.4100, 1 hit |
InterProi | View protein in InterPro IPR042235, ZP-C IPR001507, ZP_dom IPR017977, ZP_dom_CS |
Pfami | View protein in Pfam PF00100, Zona_pellucida, 1 hit |
PRINTSi | PR00023, ZPELLUCIDA |
SMARTi | View protein in SMART SM00241, ZP, 1 hit |
PROSITEi | View protein in PROSITE PS00682, ZP_1, 1 hit PS51034, ZP_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MACKQKGDSG SPSSRFSADW STYRSLSLFF ILVTSVNSVG VMQLVNPIFP
60 70 80 90 100
GTVICHENKM TVEFPRDLGT KKWHASVVDP FSFELLNCTS ILDPEKLTLK
110 120 130 140 150
APYETCSRRV LGQHQMAIRL TDNNAASRHK AFMYQISCPV MQTEETHEHA
160 170 180 190 200
GSTICTKDSM SFTFNIIPGM ADENTNPSGG KWMMEVDDAK AQNLTLREAL
210 220 230 240 250
MQGYNFLFDS HRLSVQVSFN ATGVTHYMQG NSHLYTVPLK LIHTSPGQKI
260 270 280 290 300
ILTTRVLCMS DPVTCNATHM TLTIPEFPGK LQSVRFENTN FRVSQLHNHG
310 320 330 340 350
IDKEELNGLR LHFSKSLLKM NSSEKCLLYQ FYLASLKLTF AFERDTVSTV
360 370 380 390 400
VYPECVCEPP VTIVTGDLCT QDGFMDVKVY SHQTKPALNL DTLRVGDSSC
410 420 430 440 450
QPTFKAPSQG LTLFHIPLNG CGTRLKFKGD TVIYENEIHA LWTDLPPSTI
460 470 480 490 500
SRDSEFRMTV KCHYSRDDLL INTNVQSLPP PVASVRPGPL ALILQTYPDK
510 520 530 540 550
SYLRPYGDKE YPVVRYLRQP IYLEVKVLNR ADPNIKLVLD DCWATPTMDP
560 570 580 590 600
ASLPQWNIVM DGCEYNLDNY RTTFHPVGSS VTYPTHYQRF DVKTFAFISE
610 620 630 640 650
AQVLSSLVYF HCTALICNRL SPDSPLCSVT CPVSSRHRRA TGSTEEEKMI
660 670 680 690 700
VSLPGPILLL ADSSSLRDGV DSKGHRAAGY VAFKTVVAVA ALAGLVAALG
710
LIIYLRKKRT MVLNH
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 15 | R → W in BAA08097 (Ref. 2) Curated | 1 | |
Sequence conflicti | 292 | R → A in BAA08097 (Ref. 2) Curated | 1 | |
Sequence conflicti | 328 | L → P in BAA08097 (Ref. 2) Curated | 1 | |
Sequence conflicti | 599 | S → A in BAA08097 (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U05779 mRNA Translation: AAA74386.1 D45069 mRNA Translation: BAA08097.1 |
PIRi | S70397 |
RefSeqi | NP_001003304.1, NM_001003304.1 |
Genome annotation databases
GeneIDi | 403988 |
KEGGi | cfa:403988 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U05779 mRNA Translation: AAA74386.1 D45069 mRNA Translation: BAA08097.1 |
PIRi | S70397 |
RefSeqi | NP_001003304.1, NM_001003304.1 |
3D structure databases
SMRi | P47983 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9615.ENSCAFP00000026286 |
Proteomic databases
PaxDbi | P47983 |
Genome annotation databases
GeneIDi | 403988 |
KEGGi | cfa:403988 |
Organism-specific databases
CTDi | 7783 |
Phylogenomic databases
eggNOGi | ENOG502QPI2, Eukaryota |
InParanoidi | P47983 |
OrthoDBi | 586615at2759 |
Family and domain databases
Gene3Di | 2.60.40.4100, 1 hit |
InterProi | View protein in InterPro IPR042235, ZP-C IPR001507, ZP_dom IPR017977, ZP_dom_CS |
Pfami | View protein in Pfam PF00100, Zona_pellucida, 1 hit |
PRINTSi | PR00023, ZPELLUCIDA |
SMARTi | View protein in SMART SM00241, ZP, 1 hit |
PROSITEi | View protein in PROSITE PS00682, ZP_1, 1 hit PS51034, ZP_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ZP2_CANLF | |
Accessioni | P47983Primary (citable) accession number: P47983 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1996 |
Last sequence update: | February 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 104 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families