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Protein

Carnitine O-acetyltransferase

Gene

Crat

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible transfer of acyl groups from carnitine to coenzyme A (CoA) and regulates the acyl-CoA/CoA ratio. Also plays a crucial role in the transport of fatty acids for beta-oxidation. May be specific for short chain fatty acids.By similarity

Catalytic activityi

Acetyl-CoA + carnitine = CoA + O-acetylcarnitine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei343Proton acceptor1
Binding sitei419Coenzyme A2 Publications1
Binding sitei452Carnitine2 Publications1
Binding sitei454Carnitine2 Publications1
Binding sitei456Coenzyme A; via amide nitrogen2 Publications1
Binding sitei465Carnitine2 Publications1
Binding sitei504Coenzyme A2 Publications1
Binding sitei555Coenzyme A; via carbonyl oxygen2 Publications1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processFatty acid metabolism, Lipid metabolism, Transport

Enzyme and pathway databases

BRENDAi2.3.1.7 3474
ReactomeiR-MMU-389887 Beta-oxidation of pristanoyl-CoA
R-MMU-9033241 Peroxisomal protein import

Names & Taxonomyi

Protein namesi
Recommended name:
Carnitine O-acetyltransferase (EC:2.3.1.7)
Short name:
Carnitine acetylase
Alternative name(s):
Carnitine acetyltransferase
Short name:
CAT
Short name:
CrAT
Gene namesi
Name:Crat
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:109501 Crat

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion inner membrane, Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi564M → A: Lowers activity towards short-chain fatty acids. 1
Mutagenesisi564M → G: Lowers activity towards short-chain fatty acids. Strong increase in activity towards medium chain fatty acids. 1
Mutagenesisi565F → A: Increases activity towards short-chain fatty acids. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002101731 – 626Carnitine O-acetyltransferaseAdd BLAST626

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei93N6-succinyllysineCombined sources1
Modified residuei261N6-acetyllysine; alternateBy similarity1
Modified residuei261N6-succinyllysine; alternateCombined sources1
Modified residuei268N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP47934
PaxDbiP47934
PRIDEiP47934

PTM databases

iPTMnetiP47934
PhosphoSitePlusiP47934

Expressioni

Gene expression databases

BgeeiENSMUSG00000026853 Expressed in 311 organ(s), highest expression level in testis
CleanExiMM_CRAT
ExpressionAtlasiP47934 baseline and differential
GenevisibleiP47934 MM

Interactioni

Subunit structurei

Monomer.2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198871, 1 interactor
IntActiP47934, 5 interactors
MINTiP47934
STRINGi10090.ENSMUSP00000028207

Structurei

Secondary structure

1626
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP47934
SMRiP47934
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP47934

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni423 – 430Coenzyme A binding8

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi624 – 626Microbody targeting signalSequence analysis3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3717 Eukaryota
ENOG410XNZ9 LUCA
GeneTreeiENSGT00760000119220
HOGENOMiHOG000233845
HOVERGENiHBG107717
InParanoidiP47934
KOiK00624
OrthoDBiEOG091G038F
PhylomeDBiP47934
TreeFamiTF313836

Family and domain databases

InterProiView protein in InterPro
IPR000542 Carn_acyl_trans
IPR039551 Cho/carn_acyl_trans
PANTHERiPTHR22589 PTHR22589, 1 hit
PfamiView protein in Pfam
PF00755 Carn_acyltransf, 1 hit
PROSITEiView protein in PROSITE
PS00439 ACYLTRANSF_C_1, 1 hit
PS00440 ACYLTRANSF_C_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 8 potential isoforms that are computationally mapped.Show allAlign All

P47934-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLAFAARTVV KPLGLLKPSS LMKVSGRFKA HQDALPRLPV PPLQQSLDYY
60 70 80 90 100
LKALQPIVSE EEWAHTKQLV DEFQTSGGVG ERLQKGLERR AKKMENWLSE
110 120 130 140 150
WWLKTAYLQF RQPVVIYSSP GVILPKQDFV DLQGQLRFAA KLIEGVLDFK
160 170 180 190 200
SMIDNETLPV EFLGGQPLCM NQYYQILSSC RVPGPKQDSV VNFLKSKRPP
210 220 230 240 250
THITVVHNYQ FFELDVYHSD GTPLTSDQIF VQLEKIWNSS LQSNKEPVGI
260 270 280 290 300
LTSNHRNTWA KAYNNLIKDK VNRESVNSIQ KSIFTVCLDK QVPRVSDDVY
310 320 330 340 350
RNHVAGQMLH GGGSKFNSGN RWFDKTLQFI VAEDGSCGMV YEHAAAEGPP
360 370 380 390 400
IVALVDHVME YTKKPELVRS PMVPLPMPKK LRFNITPEIK NDIEKAKQNL
410 420 430 440 450
SIMIQDLDIM MLTFHHFGKD FPKSEKLSPD AFIQVALQLA YYRIYGQACA
460 470 480 490 500
TYESASLRMF HLGRTDTIRS ASIDSLAFVK GMGDSTVPEQ QKVELLRKAV
510 520 530 540 550
QAHRAYTDRA IRGEAFDRHL LGLKLQAIED LVSMPDIFMD TSYAIAMHFN
560 570 580 590 600
LSTSQVPAKT DCVMFFGPVV PDGYGICYNP MEAHINFSVS AYNSCAETNA
610 620
ARMAHYLEKA LLDMRTLLQN HPRAKL
Length:626
Mass (Da):70,840
Last modified:January 11, 2011 - v3
Checksum:i975EA04403A4F3D9
GO

Computationally mapped potential isoform sequencesi

There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H7BX88H7BX88_MOUSE
Carnitine O-acetyltransferase
Crat
605Annotation score:
A2AWJ5A2AWJ5_MOUSE
Carnitine O-acetyltransferase
Crat
132Annotation score:
B7ZDD7B7ZDD7_MOUSE
Carnitine O-acetyltransferase
Crat
205Annotation score:
F7BF80F7BF80_MOUSE
Carnitine O-acetyltransferase
Crat
201Annotation score:
A2AWE5A2AWE5_MOUSE
Carnitine O-acetyltransferase
Crat
61Annotation score:
B7ZDD8B7ZDD8_MOUSE
Carnitine O-acetyltransferase
Crat
44Annotation score:
B7ZDD9B7ZDD9_MOUSE
Carnitine O-acetyltransferase
Crat
58Annotation score:
D6REV7D6REV7_MOUSE
Carnitine O-acetyltransferase
Crat
36Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti39P → S in BAE41993 (PubMed:16141072).Curated1
Sequence conflicti110 – 112FRQ → LPDK in CAA59971 (PubMed:9065756).Curated3
Sequence conflicti182V → E in CAA59971 (PubMed:9065756).Curated1
Sequence conflicti217Y → H in BAE41993 (PubMed:16141072).Curated1
Sequence conflicti218H → N in CAA59971 (PubMed:9065756).Curated1
Sequence conflicti587F → L in CAA59971 (PubMed:9065756).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85983 mRNA Translation: CAA59971.1
AK132179 mRNA Translation: BAE21016.1
AK170740 mRNA Translation: BAE41993.1
AL954388, AL954299 Genomic DNA Translation: CAM23162.1
AL954299, AL954388 Genomic DNA Translation: CAM23291.1
BC006668 mRNA Translation: AAH06668.1
CCDSiCCDS15882.1
PIRiS52782
RefSeqiNP_031786.2, NM_007760.3
UniGeneiMm.20396

Genome annotation databases

EnsembliENSMUST00000028207; ENSMUSP00000028207; ENSMUSG00000026853
ENSMUST00000102855; ENSMUSP00000099919; ENSMUSG00000026853
GeneIDi12908
KEGGimmu:12908
UCSCiuc008jcl.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85983 mRNA Translation: CAA59971.1
AK132179 mRNA Translation: BAE21016.1
AK170740 mRNA Translation: BAE41993.1
AL954388, AL954299 Genomic DNA Translation: CAM23162.1
AL954299, AL954388 Genomic DNA Translation: CAM23291.1
BC006668 mRNA Translation: AAH06668.1
CCDSiCCDS15882.1
PIRiS52782
RefSeqiNP_031786.2, NM_007760.3
UniGeneiMm.20396

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NDBX-ray1.80A/B30-625[»]
1NDFX-ray1.90A/B30-625[»]
1NDIX-ray2.30A/B30-625[»]
1T7NX-ray1.90A30-626[»]
1T7OX-ray2.30A30-626[»]
1T7QX-ray1.80A/B30-626[»]
2H3PX-ray2.20A/B30-625[»]
2H3UX-ray1.90A/B30-625[»]
2H3WX-ray2.10A/B30-625[»]
ProteinModelPortaliP47934
SMRiP47934
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198871, 1 interactor
IntActiP47934, 5 interactors
MINTiP47934
STRINGi10090.ENSMUSP00000028207

PTM databases

iPTMnetiP47934
PhosphoSitePlusiP47934

Proteomic databases

MaxQBiP47934
PaxDbiP47934
PRIDEiP47934

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028207; ENSMUSP00000028207; ENSMUSG00000026853
ENSMUST00000102855; ENSMUSP00000099919; ENSMUSG00000026853
GeneIDi12908
KEGGimmu:12908
UCSCiuc008jcl.1 mouse

Organism-specific databases

CTDi1384
MGIiMGI:109501 Crat

Phylogenomic databases

eggNOGiKOG3717 Eukaryota
ENOG410XNZ9 LUCA
GeneTreeiENSGT00760000119220
HOGENOMiHOG000233845
HOVERGENiHBG107717
InParanoidiP47934
KOiK00624
OrthoDBiEOG091G038F
PhylomeDBiP47934
TreeFamiTF313836

Enzyme and pathway databases

BRENDAi2.3.1.7 3474
ReactomeiR-MMU-389887 Beta-oxidation of pristanoyl-CoA
R-MMU-9033241 Peroxisomal protein import

Miscellaneous databases

EvolutionaryTraceiP47934
PROiPR:P47934
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026853 Expressed in 311 organ(s), highest expression level in testis
CleanExiMM_CRAT
ExpressionAtlasiP47934 baseline and differential
GenevisibleiP47934 MM

Family and domain databases

InterProiView protein in InterPro
IPR000542 Carn_acyl_trans
IPR039551 Cho/carn_acyl_trans
PANTHERiPTHR22589 PTHR22589, 1 hit
PfamiView protein in Pfam
PF00755 Carn_acyltransf, 1 hit
PROSITEiView protein in PROSITE
PS00439 ACYLTRANSF_C_1, 1 hit
PS00440 ACYLTRANSF_C_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCACP_MOUSE
AccessioniPrimary (citable) accession number: P47934
Secondary accession number(s): Q3TCG3, Q3V1Y3, Q923A6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 11, 2011
Last modified: November 7, 2018
This is version 148 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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