Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Floral homeotic protein APETALA 2

Gene

AP2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable transcriptional activator that promotes early floral meristem identity (PubMed:7919989). Is required subsequently for the transition of an inflorescence meristem into a floral meristem (PubMed:1675158). Plays a central role in the specification of floral identity, particularly for the normal development of sepals and petals in the wild-type flower, by spatially controlling the expression domains of multiple floral organ identity genes (PubMed:1675158, PubMed:23034631). Acts as A class cadastral protein by repressing the C class floral homeotic gene AGAMOUS in association with other repressors like LEUNIG and SEUSS (PubMed:1675158). Directly represses AGAMOUS by recruiting the transcriptional corepressor TOPLESS and the histone deacetylase HDA19 (PubMed:23034631). It is also required during seed development (PubMed:1675158).3 Publications

Miscellaneous

Mutations in the APETALA2 gene result in the ectopic expression of AGAMOUS, leading to the replacement of sepals by carpels and stamens and of petals by stamens.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi131 – 187AP2/ERF 1PROSITE-ProRule annotationAdd BLAST57
DNA bindingi223 – 280AP2/ERF 2PROSITE-ProRule annotationAdd BLAST58

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • DNA binding transcription factor activity Source: TAIR

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • flower development Source: TAIR
  • meristem maintenance Source: TAIR
  • plant ovule development Source: TAIR
  • seed development Source: TAIR
  • specification of floral organ identity Source: CACAO
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processDifferentiation, Flowering, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Floral homeotic protein APETALA 21 Publication
Gene namesi
Name:AP21 Publication
Ordered Locus Names:At4g36920Imported
ORF Names:AP22.49, C7A10.440Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G36920
TAIRilocus:2005493 AT4G36920

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi159G → E in AP2-5; temperature-sensitive. 1 Publication1
Mutagenesisi251G → S in AP2-1; temperature-sensitive. 1 Publication1
Mutagenesisi304 – 308LDLSL → ADASA: Loss of interaction with TPL. 1 Publication5
Mutagenesisi420Q → E in AP2-5; temperature-sensitive. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001125221 – 432Floral homeotic protein APETALA 2Add BLAST432

Proteomic databases

PaxDbiP47927

Expressioni

Tissue specificityi

Sepals, petals, stamens, carpels, developing ovules, inflorescence stem, leaf and stem.

Developmental stagei

It is detectable at low levels throughout the shoot apex and at enhanced levels in the inflorescence meristem, young floral buds and throughout the early stages of flower development and organogenesis. During floral organ differentiation it becomes spatially restricted to specific organ, tissue and cell types within the flower.

Inductioni

Negatively regulated by the C class floral homeotic protein AGAMOUS in stamens and carpels. MicroRNA 172 (miRNA172) negatively regulates APETALA2 at the translational level and may modulate its expression pattern. Seems not to be influenced by jasmonate and Alternaria brassicicola.3 Publications

Gene expression databases

ExpressionAtlasiP47927 baseline and differential
GenevisibleiP47927 AT

Interactioni

Subunit structurei

May form homodimer. Interacts with HDA19 and with TPL in an EAR-motif dependent manner (PubMed:23034631).1 Publication

Protein-protein interaction databases

BioGridi15126, 4 interactors
STRINGi3702.AT4G36920.1

Structurei

3D structure databases

ProteinModelPortaliP47927
SMRiP47927
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi119 – 128Nuclear localization signalSequence analysis10
Motifi304 – 308EARCurated5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi14 – 50Asp/Glu/Ser-rich (acidic; potential involvement with transcription)Sequence analysisAdd BLAST37

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IEQU Eukaryota
ENOG410YBZ8 LUCA
HOGENOMiHOG000033977
InParanoidiP47927
KOiK09284
OMAiPGFPRSH
OrthoDBiEOG09360CM4
PhylomeDBiP47927

Family and domain databases

CDDicd00018 AP2, 2 hits
Gene3Di3.30.730.10, 2 hits
InterProiView protein in InterPro
IPR001471 AP2/ERF_dom
IPR036955 AP2/ERF_dom_sf
IPR016177 DNA-bd_dom_sf
PfamiView protein in Pfam
PF00847 AP2, 2 hits
PRINTSiPR00367 ETHRSPELEMNT
SMARTiView protein in SMART
SM00380 AP2, 2 hits
SUPFAMiSSF54171 SSF54171, 2 hits
PROSITEiView protein in PROSITE
PS51032 AP2_ERF, 2 hits

Sequencei

Sequence statusi: Complete.

P47927-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWDLNDAPHQ TQREEESEEF CYSSPSKRVG SFSNSSSSAV VIEDGSDDDE
60 70 80 90 100
LNRVRPNNPL VTHQFFPEMD SNGGGVASGF PRAHWFGVKF CQSDLATGSS
110 120 130 140 150
AGKATNVAAA VVEPAQPLKK SRRGPRSRSS QYRGVTFYRR TGRWESHIWD
160 170 180 190 200
CGKQVYLGGF DTAHAAARAY DRAAIKFRGV EADINFNIDD YDDDLKQMTN
210 220 230 240 250
LTKEEFVHVL RRQSTGFPRG SSKYRGVTLH KCGRWEARMG QFLGKKYVYL
260 270 280 290 300
GLFDTEVEAA RAYDKAAIKC NGKDAVTNFD PSIYDEELNA ESSGNPTTPQ
310 320 330 340 350
DHNLDLSLGN SANSKHKSQD MRLRMNQQQQ DSLHSNEVLG LGQTGMLNHT
360 370 380 390 400
PNSNHQFPGS SNIGSGGGFS LFPAAENHRF DGRASTNQVL TNAAASSGFS
410 420 430
PHHHNQIFNS TSTPHQNWLQ TNGFQPPLMR PS
Length:432
Mass (Da):47,833
Last modified:February 1, 1996 - v1
Checksum:i376A355291EFFB42
GO

Sequence cautioni

The sequence AAM91531 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12546 mRNA Translation: AAC13770.1
Z99707 Genomic DNA Translation: CAB16765.1
AL161590 Genomic DNA Translation: CAB80358.1
CP002687 Genomic DNA Translation: AEE86717.1
CP002687 Genomic DNA Translation: AEE86718.1
AY128328 mRNA Translation: AAM91531.1 Different initiation.
PIRiA85436
RefSeqiNP_001190938.1, NM_001204009.1
NP_195410.1, NM_119856.3
UniGeneiAt.4638

Genome annotation databases

EnsemblPlantsiAT4G36920.1; AT4G36920.1; AT4G36920
AT4G36920.2; AT4G36920.2; AT4G36920
GeneIDi829845
GrameneiAT4G36920.1; AT4G36920.1; AT4G36920
AT4G36920.2; AT4G36920.2; AT4G36920
KEGGiath:AT4G36920

Similar proteinsi

Entry informationi

Entry nameiAP2_ARATH
AccessioniPrimary (citable) accession number: P47927
Secondary accession number(s): Q8L7P9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: July 18, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health