UniProtKB - P47878 (IBP3_MOUSE)
Insulin-like growth factor-binding protein 3
Igfbp3
Functioni
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. Promotes testicular germ cell apoptosis.
By similarityGO - Molecular functioni
- fibronectin binding Source: MGI
- insulin-like growth factor binding Source: MGI
- insulin-like growth factor I binding Source: MGI
- insulin-like growth factor II binding Source: MGI
- protein tyrosine phosphatase activator activity Source: UniProtKB
GO - Biological processi
- apoptotic process Source: UniProtKB-KW
- negative regulation of cell population proliferation Source: MGI
- negative regulation of protein phosphorylation Source: MGI
- negative regulation of smooth muscle cell migration Source: MGI
- negative regulation of smooth muscle cell proliferation Source: MGI
- negative regulation of testosterone secretion Source: MGI
- osteoblast differentiation Source: BHF-UCL
- positive regulation of apoptotic DNA fragmentation Source: MGI
- positive regulation of apoptotic process Source: UniProtKB
- positive regulation of cardiac muscle cell apoptotic process Source: MGI
- positive regulation of insulin-like growth factor receptor signaling pathway Source: MGI
- positive regulation of MAPK cascade Source: MGI
- positive regulation of myoblast differentiation Source: UniProtKB
- protein phosphorylation Source: MGI
- regulation of cell growth Source: MGI
- regulation of glucose metabolic process Source: MGI
- regulation of growth Source: MGI
- regulation of insulin-like growth factor receptor signaling pathway Source: GO_Central
- type B pancreatic cell proliferation Source: MGI
Keywordsi
Molecular function | Growth factor binding |
Biological process | Apoptosis |
Enzyme and pathway databases
Reactomei | R-MMU-381426, Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) R-MMU-6803211, TP53 Regulates Transcription of Death Receptors and Ligands R-MMU-8957275, Post-translational protein phosphorylation |
Protein family/group databases
MEROPSi | I31.952 |
Names & Taxonomyi
Protein namesi | Recommended name: Insulin-like growth factor-binding protein 3Short name: IBP-3 Short name: IGF-binding protein 3 Short name: IGFBP-3 |
Gene namesi | Name:Igfbp3 Synonyms:Igfbp-3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:96438, Igfbp3 |
VEuPathDBi | HostDB:ENSMUSG00000020427 |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular space Source: MGI
- insulin-like growth factor binary complex Source: MGI
- insulin-like growth factor ternary complex Source: BHF-UCL
Nucleus
- nucleus Source: MGI
Other locations
- heterochromatin Source: MGI
- platelet alpha granule Source: MGI
Keywords - Cellular componenti
SecretedPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 27 | By similarityAdd BLAST | 27 | |
ChainiPRO_0000014379 | 28 – 292 | Insulin-like growth factor-binding protein 3Add BLAST | 265 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 118 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 137 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 149 | PhosphoserineBy similarity | 1 | |
Glycosylationi | 200 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 202 | PhosphoserineBy similarity | 1 | |
Disulfide bondi | 214 ↔ 241 | PROSITE-ProRule annotation | ||
Disulfide bondi | 252 ↔ 263 | PROSITE-ProRule annotation | ||
Disulfide bondi | 265 ↔ 286 | PROSITE-ProRule annotation |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
CPTACi | non-CPTAC-3300 |
PaxDbi | P47878 |
PeptideAtlasi | P47878 |
PRIDEi | P47878 |
ProteomicsDBi | 267036 |
PTM databases
GlyGeni | P47878, 3 sites |
iPTMneti | P47878 |
PhosphoSitePlusi | P47878 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000020427, Expressed in decidua and 309 other tissues |
Genevisiblei | P47878, MM |
Interactioni
Subunit structurei
Interacts with XLKD1. Binds IGF2 more than IGF1.
Forms a ternary complex of about 140 to 150 kDa with IGF1 or IGF2 and a 85 kDa glycoprotein (ALS).
Interacts with TMEM219 (By similarity).
By similarityGO - Molecular functioni
- fibronectin binding Source: MGI
- insulin-like growth factor binding Source: MGI
- insulin-like growth factor I binding Source: MGI
- insulin-like growth factor II binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 200555, 1 interactor |
IntActi | P47878, 1 interactor |
STRINGi | 10090.ENSMUSP00000020702 |
Miscellaneous databases
RNActi | P47878, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 36 – 119 | IGFBP N-terminalPROSITE-ProRule annotationAdd BLAST | 84 | |
Domaini | 211 – 286 | Thyroglobulin type-1PROSITE-ProRule annotationAdd BLAST | 76 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 128 – 152 | DisorderedSequence analysisAdd BLAST | 25 | |
Regioni | 178 – 212 | DisorderedSequence analysisAdd BLAST | 35 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 191 – 207 | Polar residuesSequence analysisAdd BLAST | 17 |
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502QWC0, Eukaryota |
GeneTreei | ENSGT00940000158092 |
HOGENOMi | CLU_070833_1_1_1 |
InParanoidi | P47878 |
OMAi | ALEQCKP |
OrthoDBi | 979270at2759 |
PhylomeDBi | P47878 |
TreeFami | TF331211 |
Family and domain databases
CDDi | cd00191, TY, 1 hit |
Gene3Di | 4.10.800.10, 1 hit |
InterProi | View protein in InterPro IPR009030, Growth_fac_rcpt_cys_sf IPR012211, IGFBP-3 IPR000867, IGFBP-like IPR022321, IGFBP_1-6_chordata IPR017891, Insulin_GF-bd_Cys-rich_CS IPR000716, Thyroglobulin_1 IPR036857, Thyroglobulin_1_sf |
PANTHERi | PTHR11551:SF3, PTHR11551:SF3, 1 hit |
Pfami | View protein in Pfam PF00219, IGFBP, 1 hit PF00086, Thyroglobulin_1, 1 hit |
PRINTSi | PR01976, IGFBPFAMILY PR01979, IGFBPFAMILY3 |
SMARTi | View protein in SMART SM00121, IB, 1 hit SM00211, TY, 1 hit |
SUPFAMi | SSF57184, SSF57184, 1 hit SSF57610, SSF57610, 1 hit |
PROSITEi | View protein in PROSITE PS00222, IGFBP_N_1, 1 hit PS51323, IGFBP_N_2, 1 hit PS00484, THYROGLOBULIN_1_1, 1 hit PS51162, THYROGLOBULIN_1_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MHPARPALWA AALTALTLLR GPPVARAGAG AVGAGPVVRC EPCDARALSQ
60 70 80 90 100
CAPPPTAPAC TELVREPGCG CCLTCALREG DACGVYTERC GTGLRCQPRP
110 120 130 140 150
AEQYPLRALL NGRGFCANAS AAGSLSTYLP SQPAPGNISE SEEEHNAGSV
160 170 180 190 200
ESQVVPSTHR VTDSKFHPLH AKMDVIKKGH ARDSQRYKVD YESQSTDTQN
210 220 230 240 250
FSSESKRETE YGPCRREMED TLNHLKFLNV LSPRGVHIPN CDKKGFYKKK
260 270 280 290
QCRPSKGRKR GFCWCVDKYG QPLPGYDTKG KDDVHCLSVQ SQ
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 26 – 28 | RAG → ELA in CAA57271 (PubMed:7529732).Curated | 3 | |
Sequence conflicti | 34 | Missing in CAA57271 (PubMed:7529732).Curated | 1 | |
Sequence conflicti | 48 | L → V in CAA57271 (PubMed:7529732).Curated | 1 | |
Sequence conflicti | 251 | Q → R in CAA57271 (PubMed:7529732).Curated | 1 | |
Sequence conflicti | 260 – 261 | RG → QS in CAA57271 (PubMed:7529732).Curated | 2 | |
Sequence conflicti | 272 | P → R in CAA57271 (PubMed:7529732).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X81581 mRNA Translation: CAA57271.1 AL607124 Genomic DNA No translation available. BC058261 mRNA Translation: AAH58261.1 |
CCDSi | CCDS24428.1 |
PIRi | I48602 |
RefSeqi | NP_032369.2, NM_008343.2 |
Genome annotation databases
Ensembli | ENSMUST00000020702; ENSMUSP00000020702; ENSMUSG00000020427 ENSMUST00000135887; ENSMUSP00000131670; ENSMUSG00000020427 |
GeneIDi | 16009 |
KEGGi | mmu:16009 |
UCSCi | uc007hzi.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X81581 mRNA Translation: CAA57271.1 AL607124 Genomic DNA No translation available. BC058261 mRNA Translation: AAH58261.1 |
CCDSi | CCDS24428.1 |
PIRi | I48602 |
RefSeqi | NP_032369.2, NM_008343.2 |
3D structure databases
SMRi | P47878 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 200555, 1 interactor |
IntActi | P47878, 1 interactor |
STRINGi | 10090.ENSMUSP00000020702 |
Protein family/group databases
MEROPSi | I31.952 |
PTM databases
GlyGeni | P47878, 3 sites |
iPTMneti | P47878 |
PhosphoSitePlusi | P47878 |
Proteomic databases
CPTACi | non-CPTAC-3300 |
PaxDbi | P47878 |
PeptideAtlasi | P47878 |
PRIDEi | P47878 |
ProteomicsDBi | 267036 |
Protocols and materials databases
Antibodypediai | 872, 722 antibodies from 43 providers |
DNASUi | 16009 |
Genome annotation databases
Ensembli | ENSMUST00000020702; ENSMUSP00000020702; ENSMUSG00000020427 ENSMUST00000135887; ENSMUSP00000131670; ENSMUSG00000020427 |
GeneIDi | 16009 |
KEGGi | mmu:16009 |
UCSCi | uc007hzi.2, mouse |
Organism-specific databases
CTDi | 3486 |
MGIi | MGI:96438, Igfbp3 |
VEuPathDBi | HostDB:ENSMUSG00000020427 |
Phylogenomic databases
eggNOGi | ENOG502QWC0, Eukaryota |
GeneTreei | ENSGT00940000158092 |
HOGENOMi | CLU_070833_1_1_1 |
InParanoidi | P47878 |
OMAi | ALEQCKP |
OrthoDBi | 979270at2759 |
PhylomeDBi | P47878 |
TreeFami | TF331211 |
Enzyme and pathway databases
Reactomei | R-MMU-381426, Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) R-MMU-6803211, TP53 Regulates Transcription of Death Receptors and Ligands R-MMU-8957275, Post-translational protein phosphorylation |
Miscellaneous databases
BioGRID-ORCSi | 16009, 2 hits in 63 CRISPR screens |
PROi | PR:P47878 |
RNActi | P47878, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000020427, Expressed in decidua and 309 other tissues |
Genevisiblei | P47878, MM |
Family and domain databases
CDDi | cd00191, TY, 1 hit |
Gene3Di | 4.10.800.10, 1 hit |
InterProi | View protein in InterPro IPR009030, Growth_fac_rcpt_cys_sf IPR012211, IGFBP-3 IPR000867, IGFBP-like IPR022321, IGFBP_1-6_chordata IPR017891, Insulin_GF-bd_Cys-rich_CS IPR000716, Thyroglobulin_1 IPR036857, Thyroglobulin_1_sf |
PANTHERi | PTHR11551:SF3, PTHR11551:SF3, 1 hit |
Pfami | View protein in Pfam PF00219, IGFBP, 1 hit PF00086, Thyroglobulin_1, 1 hit |
PRINTSi | PR01976, IGFBPFAMILY PR01979, IGFBPFAMILY3 |
SMARTi | View protein in SMART SM00121, IB, 1 hit SM00211, TY, 1 hit |
SUPFAMi | SSF57184, SSF57184, 1 hit SSF57610, SSF57610, 1 hit |
PROSITEi | View protein in PROSITE PS00222, IGFBP_N_1, 1 hit PS51323, IGFBP_N_2, 1 hit PS00484, THYROGLOBULIN_1_1, 1 hit PS51162, THYROGLOBULIN_1_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | IBP3_MOUSE | |
Accessioni | P47878Primary (citable) accession number: P47878 Secondary accession number(s): Q6PE62 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1996 |
Last sequence update: | October 16, 2013 | |
Last modified: | February 23, 2022 | |
This is version 166 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot