UniProtKB - P47864 (AQP5_RAT)
Protein
Aquaporin-5
Gene
Aqp5
Organism
Rattus norvegicus (Rat)
Status
Functioni
Forms a water-specific channel (PubMed:7530250). Plays an important role in fluid secretion in salivary glands. Required for TRPV4 activation by hypotonicity. Together with TRPV4, controls regulatory volume decrease in salivary epithelial cells. Seems to play a redundant role in water transport in the eye, lung and in sweat glands (By similarity).By similarity1 Publication
GO - Molecular functioni
- identical protein binding Source: RGD
- water channel activity Source: UniProtKB
GO - Biological processi
- camera-type eye morphogenesis Source: RGD
- carbon dioxide transport Source: UniProtKB
- cellular hypotonic response Source: UniProtKB
- odontogenesis Source: RGD
- pancreatic juice secretion Source: RGD
- protein homotetramerization Source: UniProtKB
- saliva secretion Source: RGD
- water transport Source: UniProtKB
Keywordsi
Biological process | Transport |
Enzyme and pathway databases
Reactomei | R-RNO-432047, Passive transport by Aquaporins |
Names & Taxonomyi
Protein namesi | Recommended name: Aquaporin-5Short name: AQP-5 |
Gene namesi | Name:Aqp5 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2144, Aqp5 |
Subcellular locationi
Plasma membrane
- Apical cell membrane By similarity; Multi-pass membrane protein By similarity
- Cell membrane 1 Publication; Multi-pass membrane protein By similarity
Other locations
- Cytoplasmic vesicle membrane By similarity; Multi-pass membrane protein By similarity
Note: Hypotonicity increases location at the cell membrane. Phosphorylation decreases location at the cell membrane.By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: RGD
Plasma Membrane
- apical plasma membrane Source: RGD
- basal plasma membrane Source: RGD
- integral component of plasma membrane Source: UniProtKB
- plasma membrane Source: RGD
Other locations
- cytoplasmic vesicle membrane Source: UniProtKB
- integral component of membrane Source: GO_Central
- microvillus Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 12 | CytoplasmicCuratedAdd BLAST | 12 | |
Transmembranei | 13 – 33 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 34 – 39 | ExtracellularCurated | 6 | |
Transmembranei | 40 – 60 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 61 – 65 | CytoplasmicCurated | 5 | |
Intramembranei | 66 – 74 | Discontinuously helicalBy similarity | 9 | |
Topological domaini | 75 – 87 | CytoplasmicCuratedAdd BLAST | 13 | |
Transmembranei | 88 – 108 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 109 – 126 | ExtracellularCuratedAdd BLAST | 18 | |
Transmembranei | 127 – 147 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 148 – 158 | CytoplasmicCuratedAdd BLAST | 11 | |
Transmembranei | 159 – 179 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 180 | ExtracellularCurated | 1 | |
Intramembranei | 181 – 191 | Discontinuously helicalBy similarityAdd BLAST | 11 | |
Topological domaini | 192 – 203 | ExtracellularCuratedAdd BLAST | 12 | |
Transmembranei | 204 – 224 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 225 – 265 | CytoplasmicCuratedAdd BLAST | 41 |
Keywords - Cellular componenti
Cell membrane, Cytoplasmic vesicle, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000063953 | 1 – 265 | Aquaporin-5Add BLAST | 265 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 124 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
PaxDbi | P47864 |
PRIDEi | P47864 |
PTM databases
GlyGeni | P47864, 1 site |
iPTMneti | P47864 |
PhosphoSitePlusi | P47864 |
Expressioni
Tissue specificityi
Salivary glands, lacrimal glands, corneal epithelium in eye, trachea and lung.1 Publication
Gene expression databases
Bgeei | ENSRNOG00000051970, Expressed in lung and 14 other tissues |
Genevisiblei | P47864, RN |
Interactioni
Subunit structurei
Homotetramer.
Interacts with TRPV4; the interaction is probably indirect and regulates TRPV4 activation by hypotonicity.
By similarityGO - Molecular functioni
- identical protein binding Source: RGD
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000024102 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 69 – 71 | NPA 1By similarity | 3 | |
Motifi | 185 – 187 | NPA 2By similarity | 3 |
Domaini
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).By similarity
Sequence similaritiesi
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0223, Eukaryota |
GeneTreei | ENSGT00940000161557 |
HOGENOMi | CLU_020019_3_3_1 |
InParanoidi | P47864 |
OMAi | AMPARRY |
OrthoDBi | 1152704at2759 |
TreeFami | TF312940 |
Family and domain databases
CDDi | cd00333, MIP, 1 hit |
Gene3Di | 1.20.1080.10, 1 hit |
InterProi | View protein in InterPro IPR023271, Aquaporin-like IPR023276, Aquaporin_5 IPR034294, Aquaporin_transptr IPR000425, MIP IPR022357, MIP_CS |
PANTHERi | PTHR19139, PTHR19139, 1 hit PTHR19139:SF38, PTHR19139:SF38, 1 hit |
Pfami | View protein in Pfam PF00230, MIP, 1 hit |
PRINTSi | PR02017, AQUAPORIN5 PR00783, MINTRINSICP |
SUPFAMi | SSF81338, SSF81338, 1 hit |
TIGRFAMsi | TIGR00861, MIP, 1 hit |
PROSITEi | View protein in PROSITE PS00221, MIP, 1 hit |
i Sequence
Sequence statusi: Complete.
P47864-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKKEVCSLAF FKAVFAEFLA TLIFVFFGLG SALKWPSALP TILQISIAFG
60 70 80 90 100
LAIGTLAQAL GPVSGGHINP AITLALLIGN QISLLRAVFY VAAQLVGAIA
110 120 130 140 150
GAGILYWLAP LNARGNLAVN ALNNNTTPGK AMVVELILTF QLALCIFSST
160 170 180 190 200
DSRRTSPVGS PALSIGLSVT LGHLVGIYFT GCSMNPARSF GPAVVMNRFS
210 220 230 240 250
PSHWVFWVGP IVGAMLAAIL YFYLLFPSSL SLHDRVAVVK GTYEPEEDWE
260
DHREERKKTI ELTAH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U16245 mRNA Translation: AAA66221.1 BC092572 mRNA Translation: AAH92572.2 BC110045 mRNA Translation: AAI10046.1 |
PIRi | A55630 |
RefSeqi | NP_036911.1, NM_012779.1 |
Genome annotation databases
Ensembli | ENSRNOT00000084911; ENSRNOP00000073596; ENSRNOG00000051970 |
GeneIDi | 25241 |
KEGGi | rno:25241 |
UCSCi | RGD:2144, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U16245 mRNA Translation: AAA66221.1 BC092572 mRNA Translation: AAH92572.2 BC110045 mRNA Translation: AAI10046.1 |
PIRi | A55630 |
RefSeqi | NP_036911.1, NM_012779.1 |
3D structure databases
SMRi | P47864 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000024102 |
PTM databases
GlyGeni | P47864, 1 site |
iPTMneti | P47864 |
PhosphoSitePlusi | P47864 |
Proteomic databases
PaxDbi | P47864 |
PRIDEi | P47864 |
Genome annotation databases
Ensembli | ENSRNOT00000084911; ENSRNOP00000073596; ENSRNOG00000051970 |
GeneIDi | 25241 |
KEGGi | rno:25241 |
UCSCi | RGD:2144, rat |
Organism-specific databases
CTDi | 362 |
RGDi | 2144, Aqp5 |
Phylogenomic databases
eggNOGi | KOG0223, Eukaryota |
GeneTreei | ENSGT00940000161557 |
HOGENOMi | CLU_020019_3_3_1 |
InParanoidi | P47864 |
OMAi | AMPARRY |
OrthoDBi | 1152704at2759 |
TreeFami | TF312940 |
Enzyme and pathway databases
Reactomei | R-RNO-432047, Passive transport by Aquaporins |
Miscellaneous databases
PROi | PR:P47864 |
Gene expression databases
Bgeei | ENSRNOG00000051970, Expressed in lung and 14 other tissues |
Genevisiblei | P47864, RN |
Family and domain databases
CDDi | cd00333, MIP, 1 hit |
Gene3Di | 1.20.1080.10, 1 hit |
InterProi | View protein in InterPro IPR023271, Aquaporin-like IPR023276, Aquaporin_5 IPR034294, Aquaporin_transptr IPR000425, MIP IPR022357, MIP_CS |
PANTHERi | PTHR19139, PTHR19139, 1 hit PTHR19139:SF38, PTHR19139:SF38, 1 hit |
Pfami | View protein in Pfam PF00230, MIP, 1 hit |
PRINTSi | PR02017, AQUAPORIN5 PR00783, MINTRINSICP |
SUPFAMi | SSF81338, SSF81338, 1 hit |
TIGRFAMsi | TIGR00861, MIP, 1 hit |
PROSITEi | View protein in PROSITE PS00221, MIP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AQP5_RAT | |
Accessioni | P47864Primary (citable) accession number: P47864 Secondary accession number(s): Q2YDV0, Q569C5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1996 |
Last sequence update: | February 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 129 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families