UniProtKB - P47852 (PRIO_ODOHE)
Major prion protein
PRNP
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 64 | Copper or zinc 1By similarity | 1 | |
Metal bindingi | 65 | Copper or zinc 1; via amide nitrogenBy similarity | 1 | |
Metal bindingi | 66 | Copper or zinc 1; via amide nitrogen and carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 72 | Copper or zinc 2By similarity | 1 | |
Metal bindingi | 73 | Copper or zinc 2; via amide nitrogenBy similarity | 1 | |
Metal bindingi | 74 | Copper or zinc 2; via amide nitrogen and carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 80 | Copper or zinc 3By similarity | 1 | |
Metal bindingi | 81 | Copper or zinc 3; via amide nitrogenBy similarity | 1 | |
Metal bindingi | 82 | Copper or zinc 3; via amide nitrogen and carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 88 | Copper or zinc 4By similarity | 1 | |
Metal bindingi | 90 | Copper or zinc 4; via amide nitrogenBy similarity | 1 | |
Metal bindingi | 91 | Copper or zinc 4; via amide nitrogen and carbonyl oxygenBy similarity | 1 |
GO - Molecular functioni
- copper ion binding Source: UniProtKB
GO - Biological processi
- protein homooligomerization Source: InterPro
Keywordsi
Molecular function | Prion |
Ligand | Copper, Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Major prion proteinShort name: PrP Alternative name(s): CD_antigen: CD230 |
Gene namesi | Name:PRNP Synonyms:PRP |
Organismi | Odocoileus hemionus (Mule deer) (Cervus hemionus) |
Taxonomic identifieri | 9872 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Cervidae › Odocoileinae › Odocoileus |
Subcellular locationi
Golgi apparatus
- Golgi apparatus By similarity
Plasma membrane
- Cell membrane By similarity; GPI-anchor By similarity
Note: Targeted to lipid rafts via association with the heparan sulfate chains of GPC1. Colocates, in the presence of Cu2+, to vesicles in para- and perinuclear regions, where both proteins undergo internalization. Heparin displaces PRNP from lipid rafts and promotes endocytosis.By similarity
Golgi apparatus
- Golgi apparatus Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- anchored component of membrane Source: UniProtKB-KW
Keywords - Cellular componenti
Amyloid, Cell membrane, Golgi apparatus, MembranePathology & Biotechi
Involvement in diseasei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 24 | By similarityAdd BLAST | 24 | |
ChainiPRO_0000025703 | 25 – 233 | Major prion proteinAdd BLAST | 209 | |
PropeptideiPRO_0000025704 | 234 – 256 | Removed in mature formSequence analysisAdd BLAST | 23 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 182 ↔ 217 | By similarity | ||
Glycosylationi | 184 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 200 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Lipidationi | 233 | GPI-anchor amidated alanineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, GPI-anchor, LipoproteinInteractioni
Subunit structurei
Monomer and homodimer. Has a tendency to aggregate into amyloid fibrils containing a cross-beta spine, formed by a steric zipper of superposed beta-strands. Soluble oligomers may represent an intermediate stage on the path to fibril formation. Copper binding may promote oligomerization.
Interacts with GRB2, APP, ERI3/PRNPIP and SYN1. Mislocalized cytosolically exposed PrP interacts with MGRN1; this interaction alters MGRN1 subcellular location and causes lysosomal enlargement.
Interacts with KIAA1191.
By similarityStructurei
Secondary structure
3D structure databases
BMRBi | P47852 |
SMRi | P47852 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 54 – 62 | 1 | 9 | |
Repeati | 63 – 70 | 2 | 8 | |
Repeati | 71 – 78 | 3 | 8 | |
Repeati | 79 – 86 | 4 | 8 | |
Repeati | 87 – 95 | 5 | 9 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 25 – 233 | Interaction with GRB2, ERI3 and SYN1By similarityAdd BLAST | 209 | |
Regioni | 54 – 95 | 5 X 8 AA tandem repeats of P-H-G-G-G-W-G-QAdd BLAST | 42 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Repeat, SignalFamily and domain databases
Gene3Di | 1.10.790.10, 1 hit |
InterProi | View protein in InterPro IPR000817, Prion IPR036924, Prion/Doppel_b-ribbon_dom_sf IPR022416, Prion/Doppel_prot_b-ribbon_dom IPR025860, Prion_N_dom |
Pfami | View protein in Pfam PF00377, Prion, 1 hit PF11587, Prion_bPrPp, 1 hit |
PRINTSi | PR00341, PRION |
SMARTi | View protein in SMART SM00157, PRP, 1 hit |
SUPFAMi | SSF54098, SSF54098, 1 hit |
PROSITEi | View protein in PROSITE PS00291, PRION_1, 1 hit PS00706, PRION_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MVKSHIGSWI LVLFVAMWSD VGLCKKRPKP GGGWNTGGSR YPGQGSPGGN
60 70 80 90 100
RYPPQGGGGW GQPHGGGWGQ PHGGGWGQPH GGGWGQPHGG GGWGQGGTHS
110 120 130 140 150
QWNKPSKPKT NMKHVAGAAA AGAVVGGLGG YMLGSAMNRP LIHFGNDYED
160 170 180 190 200
RYYRENMYRY PNQVYYRPVD QYNNQNTFVH DCVNITVKQH TVTTTTKGEN
210 220 230 240 250
FTETDIKMME RVVEQMCITQ YQRESQAYYQ RGASVILFSS PPVILLISFL
IFLIVG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U25965 Genomic DNA Translation: AAA68941.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U25965 Genomic DNA Translation: AAA68941.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4YXH | X-ray | 2.70 | A | 124-231 | [»] | |
6FNV | NMR | - | A | 94-233 | [»] | |
BMRBi | P47852 | |||||
SMRi | P47852 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Family and domain databases
Gene3Di | 1.10.790.10, 1 hit |
InterProi | View protein in InterPro IPR000817, Prion IPR036924, Prion/Doppel_b-ribbon_dom_sf IPR022416, Prion/Doppel_prot_b-ribbon_dom IPR025860, Prion_N_dom |
Pfami | View protein in Pfam PF00377, Prion, 1 hit PF11587, Prion_bPrPp, 1 hit |
PRINTSi | PR00341, PRION |
SMARTi | View protein in SMART SM00157, PRP, 1 hit |
SUPFAMi | SSF54098, SSF54098, 1 hit |
PROSITEi | View protein in PROSITE PS00291, PRION_1, 1 hit PS00706, PRION_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PRIO_ODOHE | |
Accessioni | P47852Primary (citable) accession number: P47852 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1996 |
Last sequence update: | February 1, 1996 | |
Last modified: | October 7, 2020 | |
This is version 98 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families