Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Angiotensin-converting enzyme

Gene

Ace

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. This GPIase activity seems to be crucial for the egg-binding ability of the sperm (By similarity).By similarity

Catalytic activityi

Release of a C-terminal dipeptide, oligopeptide-|-Xaa-Yaa, when Xaa is not Pro, and Yaa is neither Asp nor Glu. Thus, conversion of angiotensin I to angiotensin II, with increase in vasoconstrictor activity, but no action on angiotensin II.

Cofactori

Protein has several cofactor binding sites:
  • Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit. Isoform Testis-specific only binds 1 Zn2+ ion per subunit.By similarity
  • chlorideBy similarityNote: Binds 3 chloride ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei237Chloride 1By similarity1
Metal bindingi396Zinc 1; catalyticBy similarity1
Active sitei3971PROSITE-ProRule annotation1
Metal bindingi400Zinc 1; catalyticBy similarity1
Metal bindingi424Zinc 1; catalyticBy similarity1
Binding sitei535Chloride 1By similarity1
Binding sitei797Chloride 2By similarity1
Binding sitei835Chloride 3By similarity1
Metal bindingi994Zinc 2; catalyticBy similarity1
Active sitei9952PROSITE-ProRule annotation1
Metal bindingi998Zinc 2; catalyticBy similarity1
Metal bindingi1022Zinc 2; catalyticBy similarity1
Binding sitei1096Chloride 2By similarity1
Binding sitei1100Chloride 2By similarity1
Binding sitei1133Chloride 3By similarity1

GO - Molecular functioni

  • carboxypeptidase activity Source: UniProtKB-KW
  • dipeptidyl-peptidase activity Source: GO_Central
  • drug binding Source: RGD
  • exopeptidase activity Source: GO_Central
  • metal ion binding Source: UniProtKB-KW
  • metallopeptidase activity Source: RGD
  • peptidyl-dipeptidase activity Source: RGD

GO - Biological processi

Keywordsi

Molecular functionCarboxypeptidase, Hydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.15.1 5301

Protein family/group databases

MEROPSiM02.001

Names & Taxonomyi

Protein namesi
Recommended name:
Angiotensin-converting enzyme (EC:3.2.1.-, EC:3.4.15.1)
Short name:
ACE
Alternative name(s):
Dipeptidyl carboxypeptidase I
Kininase II
CD_antigen: CD143
Cleaved into the following chain:
Gene namesi
Name:Ace
Synonyms:Dcp1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2493 Ace

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini36 – 1265ExtracellularSequence analysisAdd BLAST1230
Transmembranei1266 – 1282HelicalSequence analysisAdd BLAST17
Topological domaini1283 – 1313CytoplasmicSequence analysisAdd BLAST31

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Secreted

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2625
GuidetoPHARMACOLOGYi1613

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 35By similarityAdd BLAST35
ChainiPRO_000002855736 – 1313Angiotensin-converting enzymeAdd BLAST1278
ChainiPRO_000002855836 – 1238Angiotensin-converting enzyme, soluble formAdd BLAST1203
PropeptideiPRO_00000285591239 – 1313Removed in secreted formBy similarityAdd BLAST75

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi44N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi60N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi80N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi117N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi152N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi166N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi324N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi515N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi683N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi701N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi720N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi766N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi948N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1197N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei1306PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by CK2 on Ser-1306; which allows membrane retention.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP47820
PRIDEiP47820

PTM databases

iPTMnetiP47820
PhosphoSitePlusiP47820
UniCarbKBiP47820

Expressioni

Tissue specificityi

Testis-specific isoform is expressed in spermatocytes, adult testis. Also expressed in brain, kidney, lung, skeletal muscle and heart.1 Publication

Inductioni

Up-regulated after myocardial infarction.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010627

Chemistry databases

BindingDBiP47820

Structurei

3D structure databases

ProteinModelPortaliP47820
SMRiP47820
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni36 – 636Peptidase M2 1Add BLAST601
Regioni637 – 1238Peptidase M2 2Add BLAST602

Sequence similaritiesi

Belongs to the peptidase M2 family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3690 Eukaryota
ENOG410XPJ3 LUCA
HOGENOMiHOG000007838
HOVERGENiHBG000264
InParanoidiP47820
KOiK01283
PhylomeDBiP47820
TreeFamiTF312861

Family and domain databases

CDDicd06461 M2_ACE, 2 hits
InterProiView protein in InterPro
IPR001548 Peptidase_M2
PANTHERiPTHR10514 PTHR10514, 2 hits
PfamiView protein in Pfam
PF01401 Peptidase_M2, 2 hits
PRINTSiPR00791 PEPDIPTASEA
PROSITEiView protein in PROSITE
PS00142 ZINC_PROTEASE, 2 hits

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform Somatic (identifier: P47820-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGAASGQRGR WPLSPPLLML SLLLLLLLPP SPAPALDPGL QPGNFSADEA
60 70 80 90 100
GAQLFADSYN SSAEVVMFQS TAASWAHDTN ITEENARLQE EAALINQEFA
110 120 130 140 150
EVWGKKAKEL YESIWQNFTD QKLRRIIGSV QTLGPANLPL TQRLQYNSLL
160 170 180 190 200
SNMSRIYSTG KVCFPNKTAT CWSLDPELTN ILASSRNYAK VLFAWEGWHD
210 220 230 240 250
AVGIPLRPLY QDFTALSNEA YRQDGFSDTG AYWRSWYESP SFEESLEHLY
260 270 280 290 300
HQVEPLYLNL HAFVRRALHR RYGDKYINLR GPIPAHLLGD MWAQSWENIY
310 320 330 340 350
DMVVPFPDKP NLDVTSTMVQ KGWNATHMFR VAEEFFTSLG LSPMPPEFWA
360 370 380 390 400
ESMLEKPADG REVVCHASAW DFYNRKDFRI KQCTRVTMDQ LSTVHHEMGH
410 420 430 440 450
VQYYLQYKDL HVSLRRGANP GFHEAIGDVL ALSVSTPAHL HKIGLLDRVA
460 470 480 490 500
NDIESDINYL LKMALEKIAF LPFGYLVDQW RWGVFSGRTP PSRYNYDWWY
510 520 530 540 550
LRTKYQGICP PVARNETHFD AGAKFHIPSV TPYIRYFVSF VLQFQFHQAL
560 570 580 590 600
CKEAGHQGPL HQCDIYQSTK AGAKLQQVLQ AGCSRPWQEV LKDLVGSDAL
610 620 630 640 650
DASALMEYFQ PVSQWLQEQN QRNGEVLGWP EYQWRPPLPD NYPEGIDLET
660 670 680 690 700
DEAKANRFVE EYDRTAKVLW NEYAEANWHY NTNITIEGSK ILLQKNKEVS
710 720 730 740 750
NHTLKYGTWA KTFDVSNFQN STIKRIIKKV QNVDRAVLPP NELEEYNQIL
760 770 780 790 800
LDMETTYSVA NVCYTNGTCL SLEPDLTNIM ATSRKYEELL WVWKSWRDKV
810 820 830 840 850
GRAILPFFPK YVDFSNKIAK LNGYSDAGDS WRSSYESDDL EQDLEKLYQE
860 870 880 890 900
LQPLYLNLHA YVRRSLHRHY GSEYINLDGP IPAHLLGNMW AQTWSNIYDL
910 920 930 940 950
VAPFPSAPSI DATEAMIKQG WTPRRIFKEA DNFFTSLGLL PVPPEFWNKS
960 970 980 990 1000
MLEKPTDGRE VVCHASAWDF YNGKDFRIKQ CTSVNMEELV IAHHEMGHIQ
1010 1020 1030 1040 1050
YFMQYKDLPV TFREGANPGF HEAIGDVLAL SVSTPKHLHS LNLLSSEGSG
1060 1070 1080 1090 1100
YEHDINFLMK MALDKIAFIP FSYLIDQWRW RVFDGSITKE NYNQEWWSLR
1110 1120 1130 1140 1150
LKYQGLCPPV PRSQGDFDPG SKFHVPANVP YIRYFISFII QFQFHEALCR
1160 1170 1180 1190 1200
AAGHTGPLYK CDIYQSKEAG KLLADAMKLG YSKQWPEAMK IITGQPNMSA
1210 1220 1230 1240 1250
SAIMNYFKPL TEWLVTENRR HGETLGWPEY TWTPNTARAE GSLPESSRVN
1260 1270 1280 1290 1300
FLGMYLEPQQ ARVGQWVLLF LGVALLVATV GLAHRLYNIH NHHSLRRPHR
1310
GPQFGSEVEL RHS
Length:1,313
Mass (Da):150,908
Last modified:February 1, 1996 - v1
Checksum:i8CB5D0015F129591
GO
Isoform Testis-specific (identifier: P47820-2) [UniParc] [UniParc]FASTAAdd to basket
Also known as: ACE-T

The sequence of this isoform differs from the canonical sequence as follows:
     1-581: Missing.
     582-646: GCSRPWQEVL...PLPDNYPEGI → MGQGWATPGL...ACLFSSSPPT

Show »
Length:775
Mass (Da):88,170
Checksum:i5967406D21CAD52A
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0A0MXV4A0A0A0MXV4_RAT
Angiotensin-converting enzyme
Ace
1,133Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti341L → F in AAG35596 (Ref. 2) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti207R → K2 Publications1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0376421 – 581Missing in isoform Testis-specific. 1 PublicationAdd BLAST581
Alternative sequenceiVSP_037643582 – 646GCSRP…YPEGI → MGQGWATPGLPRFLFLLLCC GHLLPVLSQVAADHVTANQG ITNQATTRSQTTHQSTISQT IQTSNGTPGRGQGHEGARSQ GPAGGNSNKTTPCGKEGEAC LFSSSPPT in isoform Testis-specific. 1 PublicationAdd BLAST65

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03708 mRNA Translation: AAA82110.1
U03734 mRNA Translation: AAA82111.1
AF201331 mRNA Translation: AAG35596.1
AF201332 mRNA Translation: AAG35597.1
AF539425 mRNA Translation: AAN17280.1
BC085760 mRNA Translation: AAH85760.1
AF532783 mRNA Translation: AAP80808.1
AF532784 mRNA Translation: AAP80809.1
PIRiJC2038
RefSeqiNP_036676.1, NM_012544.1
UniGeneiRn.10149

Genome annotation databases

GeneIDi24310
KEGGirno:24310
UCSCiRGD:2493 rat [P47820-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03708 mRNA Translation: AAA82110.1
U03734 mRNA Translation: AAA82111.1
AF201331 mRNA Translation: AAG35596.1
AF201332 mRNA Translation: AAG35597.1
AF539425 mRNA Translation: AAN17280.1
BC085760 mRNA Translation: AAH85760.1
AF532783 mRNA Translation: AAP80808.1
AF532784 mRNA Translation: AAP80809.1
PIRiJC2038
RefSeqiNP_036676.1, NM_012544.1
UniGeneiRn.10149

3D structure databases

ProteinModelPortaliP47820
SMRiP47820
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010627

Chemistry databases

BindingDBiP47820
ChEMBLiCHEMBL2625
GuidetoPHARMACOLOGYi1613

Protein family/group databases

MEROPSiM02.001

PTM databases

iPTMnetiP47820
PhosphoSitePlusiP47820
UniCarbKBiP47820

Proteomic databases

PaxDbiP47820
PRIDEiP47820

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24310
KEGGirno:24310
UCSCiRGD:2493 rat [P47820-1]

Organism-specific databases

CTDi1636
RGDi2493 Ace

Phylogenomic databases

eggNOGiKOG3690 Eukaryota
ENOG410XPJ3 LUCA
HOGENOMiHOG000007838
HOVERGENiHBG000264
InParanoidiP47820
KOiK01283
PhylomeDBiP47820
TreeFamiTF312861

Enzyme and pathway databases

BRENDAi3.4.15.1 5301

Miscellaneous databases

PROiPR:P47820

Family and domain databases

CDDicd06461 M2_ACE, 2 hits
InterProiView protein in InterPro
IPR001548 Peptidase_M2
PANTHERiPTHR10514 PTHR10514, 2 hits
PfamiView protein in Pfam
PF01401 Peptidase_M2, 2 hits
PRINTSiPR00791 PEPDIPTASEA
PROSITEiView protein in PROSITE
PS00142 ZINC_PROTEASE, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiACE_RAT
AccessioniPrimary (citable) accession number: P47820
Secondary accession number(s): Q7TMC6, Q8CFN1, Q9EQM9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 7, 2018
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again