Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glial fibrillary acidic protein

Gene

Gfap

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

GO - Molecular functioni

  • identical protein binding Source: Ensembl
  • integrin binding Source: MGI
  • kinase binding Source: RGD
  • structural constituent of cytoskeleton Source: RGD

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Glial fibrillary acidic protein
Short name:
GFAP
Gene namesi
Name:Gfap
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi2679 Gfap

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Intermediate filament

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000638071 – 430Glial fibrillary acidic proteinAdd BLAST430

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei7Phosphothreonine; by AURKB and ROCK1By similarity1
Modified residuei11Omega-N-methylarginineBy similarity1
Modified residuei12PhosphoserineCombined sources1
Modified residuei21Omega-N-methylarginineBy similarity1
Modified residuei34CitrullineBy similarity1
Modified residuei36Phosphoserine; by AURKB and ROCK1By similarity1
Modified residuei41PhosphothreonineBy similarity1
Modified residuei80PhosphoserineCombined sources1
Modified residuei108Phosphothreonine1 Publication1
Modified residuei148PhosphothreonineCombined sources1
Modified residuei267PhosphoserineCombined sources1
Modified residuei268CitrullineBy similarity1
Modified residuei321PhosphoserineCombined sources1
Modified residuei381Phosphothreonine1 Publication1
Modified residuei383PhosphoserineCombined sources1
Modified residuei404CitrullineBy similarity1
Modified residuei414CitrullineBy similarity1

Post-translational modificationi

Phosphorylated by PKN1.By similarity

Keywords - PTMi

Citrullination, Methylation, Phosphoprotein

Proteomic databases

PaxDbiP47819
PRIDEiP47819

2D gel databases

World-2DPAGEi0004:P47819

PTM databases

iPTMnetiP47819
PhosphoSitePlusiP47819
SwissPalmiP47819

Expressioni

Tissue specificityi

Expressed in the cortex and hippocampus. Expression decreases following acute and chronic corticosterone treatment.1 Publication

Gene expression databases

BgeeiENSRNOG00000002919
GenevisibleiP47819 RN

Interactioni

Subunit structurei

Interacts with SYNM.By similarity

GO - Molecular functioni

  • identical protein binding Source: Ensembl
  • integrin binding Source: MGI
  • kinase binding Source: RGD

Protein-protein interaction databases

BioGridi246558, 3 interactors
IntActiP47819, 2 interactors
MINTiP47819
STRINGi10116.ENSRNOP00000032389

Structurei

3D structure databases

ProteinModelPortaliP47819
SMRiP47819
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini67 – 375IF rodPROSITE-ProRule annotationAdd BLAST309

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 70HeadAdd BLAST70
Regioni71 – 102Coil 1AAdd BLAST32
Regioni103 – 113Linker 1Add BLAST11
Regioni114 – 212Coil 1BAdd BLAST99
Regioni213 – 228Linker 12Add BLAST16
Regioni229 – 250Coil 2AAdd BLAST22
Regioni251 – 254Linker 24
Regioni255 – 375Coil 2BAdd BLAST121
Regioni376 – 430TailAdd BLAST55

Sequence similaritiesi

Belongs to the intermediate filament family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IJXF Eukaryota
ENOG410Y9QE LUCA
GeneTreeiENSGT00910000143989
HOGENOMiHOG000230977
HOVERGENiHBG013015
InParanoidiP47819
KOiK05640
OMAiMQETEEW
OrthoDBiEOG091G12MK
PhylomeDBiP47819

Family and domain databases

InterProiView protein in InterPro
IPR027701 GFAP
IPR001664 IF
IPR018039 IF_conserved
IPR039008 IF_rod_dom
IPR006821 Intermed_filament_DNA-bd
PANTHERiPTHR23239 PTHR23239, 1 hit
PTHR23239:SF41 PTHR23239:SF41, 1 hit
PfamiView protein in Pfam
PF00038 Filament, 1 hit
PF04732 Filament_head, 1 hit
SMARTiView protein in SMART
SM01391 Filament, 1 hit
PROSITEiView protein in PROSITE
PS00226 IF_ROD_1, 1 hit
PS51842 IF_ROD_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P47819-1) [UniParc]FASTAAdd to basket
Also known as: GFAP alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MERRRITSAR RSYASSETMV RGHGPTRHLG TIPRLSLSRM TPPLPARVDF
60 70 80 90 100
SLAGALNAGF KETRASERAE MMELNDRFAS YIEKVRFLEQ QNKALAAELN
110 120 130 140 150
QLRAKEPTKL ADVYQAELRE LRLRLDQLTT NSARLEVERD NLTQDLGTLR
160 170 180 190 200
QKLQDETNLR LEAENNLAVY RQEADEATLA RVDLERKVES LEEEIQFLRK
210 220 230 240 250
IHEEEVRELQ EQLAQQQVHV EMDVAKPDLT AALREIRTQY EAVATSNMQE
260 270 280 290 300
TEEWYRSKFA DLTDVASRNA ELLRQAKHEA NDYRRQLQAL TCDLESLRGT
310 320 330 340 350
NESLERQMRE QEERHARESA SYQEALARLE EEGQSLKEEM ARHLQEYQDL
360 370 380 390 400
LNVKLALDIE IATYRKLLEG EENRITIPVQ TFSNLQIRET SLDTKSVSEG
410 420 430
HLKRNIVVKT VEMRDGEVIK ESKQEHKDVM
Length:430
Mass (Da):49,957
Last modified:September 19, 2006 - v2
Checksum:i6437A1F784E37D1A
GO
Isoform 2 (identifier: P47819-2) [UniParc]FASTAAdd to basket
Also known as: GFAP delta, GFAP epsilon

The sequence of this isoform differs from the canonical sequence as follows:
     389-430: ETSLDTKSVSEGHLKRNIVVKTVEMRDGEVIKESKQEHKDVM → GGKSTKEGEGHKVTRHLKRLTIQVIPIQALARL

Show »
Length:421
Mass (Da):48,782
Checksum:iD6FAC59B9BD290FF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti273L → V in AAA20540 (PubMed:1629938).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_017054389 – 430ETSLD…HKDVM → GGKSTKEGEGHKVTRHLKRL TIQVIPIQALARL in isoform 2. CuratedAdd BLAST42

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03700 mRNA Translation: AAA20540.1
AF028784 Genomic DNA Translation: AAD01873.1
AF028784 Genomic DNA Translation: AAD01874.2
BC088851 mRNA Translation: AAH88851.1
Z48978 Genomic DNA Translation: CAA88842.1
AY142198 Genomic DNA Translation: AAN87911.1
PIRiI56572
RefSeqiNP_058705.2, NM_017009.2 [P47819-1]
UniGeneiRn.91512

Genome annotation databases

EnsembliENSRNOT00000034401; ENSRNOP00000032389; ENSRNOG00000002919 [P47819-1]
GeneIDi24387
KEGGirno:24387
UCSCiRGD:2679 rat [P47819-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGFAP_RAT
AccessioniPrimary (citable) accession number: P47819
Secondary accession number(s): Q7TQ31, Q9R1Q3, Q9Z2S0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: September 19, 2006
Last modified: June 20, 2018
This is version 148 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health