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Protein

Glutathione reductase, mitochondrial

Gene

Gsr

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Maintains high levels of reduced glutathione in the cytosol.

Miscellaneous

The active site is a redox-active disulfide bond.

Catalytic activityi

2 glutathione + NADP+ = glutathione disulfide + NADPH.

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei489Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi72 – 80FADBy similarity9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein, NADP

Enzyme and pathway databases

ReactomeiR-MMU-3299685 Detoxification of Reactive Oxygen Species
R-MMU-499943 Interconversion of nucleotide di- and triphosphates
R-MMU-5628897 TP53 Regulates Metabolic Genes

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione reductase, mitochondrial (EC:1.8.1.7)
Short name:
GR
Short name:
GRase
Gene namesi
Name:Gsr
Synonyms:Gr1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:95804 Gsr

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2366476

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 26MitochondrionSequence analysisAdd BLAST26
ChainiPRO_000003027827 – 500Glutathione reductase, mitochondrialAdd BLAST474

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei75N6-acetyllysineCombined sources1
Disulfide bondi80 ↔ 85Redox-activeBy similarity
Disulfide bondi112InterchainBy similarity

Keywords - PTMi

Acetylation, Disulfide bond

Proteomic databases

MaxQBiP47791
PaxDbiP47791
PeptideAtlasiP47791
PRIDEiP47791

PTM databases

iPTMnetiP47791
PhosphoSitePlusiP47791
SwissPalmiP47791

Expressioni

Gene expression databases

BgeeiENSMUSG00000031584 Expressed in 292 organ(s), highest expression level in blood
CleanExiMM_GSR
GenevisibleiP47791 MM

Interactioni

Subunit structurei

Homodimer; disulfide-linked.

GO - Molecular functioni

Protein-protein interaction databases

IntActiP47791, 3 interactors
MINTiP47791
STRINGi10090.ENSMUSP00000033992

Structurei

3D structure databases

ProteinModelPortaliP47791
SMRiP47791
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Each subunit can be divided into 4 domains that are consecutive along the polypeptide chain. Domains 1 and 2 bind FAD and NADPH, respectively. Domain 4 forms the interface.

Sequence similaritiesi

Keywords - Domaini

Redox-active center, Transit peptide

Phylogenomic databases

eggNOGiKOG0405 Eukaryota
COG1249 LUCA
GeneTreeiENSGT00390000007578
HOGENOMiHOG000276712
HOVERGENiHBG004959
InParanoidiP47791
KOiK00383
OMAiKCAIIEA
OrthoDBiEOG091G078A
PhylomeDBiP47791
TreeFamiTF105353

Family and domain databases

Gene3Di3.30.390.30, 1 hit
3.50.50.60, 2 hits
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR023753 FAD/NAD-binding_dom
IPR016156 FAD/NAD-linked_Rdtase_dimer_sf
IPR006322 Glutathione_Rdtase_euk/bac
IPR001100 Pyr_nuc-diS_OxRdtase
IPR004099 Pyr_nucl-diS_OxRdtase_dimer
IPR012999 Pyr_OxRdtase_I_AS
PANTHERiPTHR42737 PTHR42737, 1 hit
PfamiView protein in Pfam
PF07992 Pyr_redox_2, 1 hit
PF02852 Pyr_redox_dim, 1 hit
PIRSFiPIRSF000350 Mercury_reductase_MerA, 1 hit
SUPFAMiSSF51905 SSF51905, 1 hit
SSF55424 SSF55424, 1 hit
TIGRFAMsiTIGR01421 gluta_reduc_1, 1 hit
PROSITEiView protein in PROSITE
PS00076 PYRIDINE_REDOX_1, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket
Isoform Mitochondrial (identifier: P47791-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MALLPRALGV GAAPSLRRAA RALTCAMASP GEPQPPAPDT SSFDYLVIGG
60 70 80 90 100
GSGGLASARR AAELGARAAV VESHKLGGTC VNVGCVPKKV MWNTAVHSEF
110 120 130 140 150
MHDHVDYGFQ SCEGKFSWHV IKQKRDAYVS RLNTIYQNNL TKSHIEIIHG
160 170 180 190 200
YATFADGPRP TVEVNGKKFT APHILIATGG VPTVPHESQI PGASLGITSD
210 220 230 240 250
GFFQLEDLPS RSVIVGAGYI AVEIAGILSA LGSKTSLMIR HDKVLRNFDS
260 270 280 290 300
LISSNCTEEL ENAGVEVLKF TQVKEVKKTS SGLELQVVTS VPGRKPTTTM
310 320 330 340 350
IPDVDCLLWA IGRDPNSKGL NLNKVGIQTD EKGHILVDEF QNTNVKGVYA
360 370 380 390 400
VGDVCGKALL TPVAIAAGRK LAHRLFECKQ DSKLDYDNIP TVVFSHPPIG
410 420 430 440 450
TVGLTEDEAV HKYGKDNVKI YSTAFTPMYH AVTTRKTKCV MKMVCANKEE
460 470 480 490 500
KVVGIHMQGI GCDEMLQGFA VAVKMGATKA DFDNTVAIHP TSSEELVTLR
Length:500
Mass (Da):53,663
Last modified:June 21, 2004 - v3
Checksum:iA2E6F629B5CC0B7B
GO
Isoform Cytoplasmic (identifier: P47791-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: Missing.

Show »
Length:474
Mass (Da):51,074
Checksum:i8E4F19F62DE1AD74
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26A → T in CAA53959 (Ref. 2) Curated1
Sequence conflicti255N → K in CAA53959 (Ref. 2) Curated1
Sequence conflicti300M → V in CAA53959 (Ref. 2) Curated1
Sequence conflicti312G → R in AAH56357 (PubMed:15489334).Curated1
Sequence conflicti312G → R in AAH57325 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0189731 – 26Missing in isoform Cytoplasmic. CuratedAdd BLAST26

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76341 mRNA Translation: CAA53959.3
AK040136 mRNA Translation: BAC30518.1
AK084328 mRNA Translation: BAC39162.1
BC056357 mRNA Translation: AAH56357.1
BC057325 mRNA Translation: AAH57325.1
CCDSiCCDS22235.1 [P47791-1]
PIRiPC4370
S39494
RefSeqiNP_034474.4, NM_010344.4 [P47791-1]
UniGeneiMm.283573

Genome annotation databases

EnsembliENSMUST00000033992; ENSMUSP00000033992; ENSMUSG00000031584 [P47791-1]
GeneIDi14782
KEGGimmu:14782
UCSCiuc009lkf.1 mouse [P47791-1]

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76341 mRNA Translation: CAA53959.3
AK040136 mRNA Translation: BAC30518.1
AK084328 mRNA Translation: BAC39162.1
BC056357 mRNA Translation: AAH56357.1
BC057325 mRNA Translation: AAH57325.1
CCDSiCCDS22235.1 [P47791-1]
PIRiPC4370
S39494
RefSeqiNP_034474.4, NM_010344.4 [P47791-1]
UniGeneiMm.283573

3D structure databases

ProteinModelPortaliP47791
SMRiP47791
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP47791, 3 interactors
MINTiP47791
STRINGi10090.ENSMUSP00000033992

Chemistry databases

ChEMBLiCHEMBL2366476

PTM databases

iPTMnetiP47791
PhosphoSitePlusiP47791
SwissPalmiP47791

Proteomic databases

MaxQBiP47791
PaxDbiP47791
PeptideAtlasiP47791
PRIDEiP47791

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033992; ENSMUSP00000033992; ENSMUSG00000031584 [P47791-1]
GeneIDi14782
KEGGimmu:14782
UCSCiuc009lkf.1 mouse [P47791-1]

Organism-specific databases

CTDi2936
MGIiMGI:95804 Gsr

Phylogenomic databases

eggNOGiKOG0405 Eukaryota
COG1249 LUCA
GeneTreeiENSGT00390000007578
HOGENOMiHOG000276712
HOVERGENiHBG004959
InParanoidiP47791
KOiK00383
OMAiKCAIIEA
OrthoDBiEOG091G078A
PhylomeDBiP47791
TreeFamiTF105353

Enzyme and pathway databases

ReactomeiR-MMU-3299685 Detoxification of Reactive Oxygen Species
R-MMU-499943 Interconversion of nucleotide di- and triphosphates
R-MMU-5628897 TP53 Regulates Metabolic Genes

Miscellaneous databases

ChiTaRSiGsr mouse
PROiPR:P47791
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031584 Expressed in 292 organ(s), highest expression level in blood
CleanExiMM_GSR
GenevisibleiP47791 MM

Family and domain databases

Gene3Di3.30.390.30, 1 hit
3.50.50.60, 2 hits
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR023753 FAD/NAD-binding_dom
IPR016156 FAD/NAD-linked_Rdtase_dimer_sf
IPR006322 Glutathione_Rdtase_euk/bac
IPR001100 Pyr_nuc-diS_OxRdtase
IPR004099 Pyr_nucl-diS_OxRdtase_dimer
IPR012999 Pyr_OxRdtase_I_AS
PANTHERiPTHR42737 PTHR42737, 1 hit
PfamiView protein in Pfam
PF07992 Pyr_redox_2, 1 hit
PF02852 Pyr_redox_dim, 1 hit
PIRSFiPIRSF000350 Mercury_reductase_MerA, 1 hit
SUPFAMiSSF51905 SSF51905, 1 hit
SSF55424 SSF55424, 1 hit
TIGRFAMsiTIGR01421 gluta_reduc_1, 1 hit
PROSITEiView protein in PROSITE
PS00076 PYRIDINE_REDOX_1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGSHR_MOUSE
AccessioniPrimary (citable) accession number: P47791
Secondary accession number(s): Q7TNC2, Q8BN97, Q8C9Z6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: June 21, 2004
Last modified: November 7, 2018
This is version 181 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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