Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Thyrotropin receptor

Gene

Tshr

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Receptor for the thyroid-stimulating hormone (TSH) or thyrotropin. Also acts as a receptor for the heterodimeric glycoprotein hormone (GPHA2:GPHB5) or thyrostimulin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Plays a central role in controlling thyroid cell metabolism.By similarity

GO - Molecular functioni

  • G protein-coupled peptide receptor activity Source: GO_Central
  • protein-containing complex binding Source: UniProtKB
  • signaling receptor activity Source: MGI
  • thyroid-stimulating hormone receptor activity Source: MGI

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-MMU-375281 Hormone ligand-binding receptors
R-MMU-418555 G alpha (s) signalling events

Names & Taxonomyi

Protein namesi
Recommended name:
Thyrotropin receptor
Alternative name(s):
Thyroid-stimulating hormone receptor
Short name:
TSH-R
Gene namesi
Name:Tshr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:98849 Tshr

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 413ExtracellularSequence analysisAdd BLAST392
Transmembranei414 – 441Helical; Name=1Sequence analysisAdd BLAST28
Topological domaini442 – 450CytoplasmicSequence analysis9
Transmembranei451 – 473Helical; Name=2Sequence analysisAdd BLAST23
Topological domaini474 – 494ExtracellularSequence analysisAdd BLAST21
Transmembranei495 – 517Helical; Name=3Sequence analysisAdd BLAST23
Topological domaini518 – 537CytoplasmicSequence analysisAdd BLAST20
Transmembranei538 – 560Helical; Name=4Sequence analysisAdd BLAST23
Topological domaini561 – 580ExtracellularSequence analysisAdd BLAST20
Transmembranei581 – 602Helical; Name=5Sequence analysisAdd BLAST22
Topological domaini603 – 625CytoplasmicSequence analysisAdd BLAST23
Transmembranei626 – 649Helical; Name=6Sequence analysisAdd BLAST24
Topological domaini650 – 660ExtracellularSequence analysisAdd BLAST11
Transmembranei661 – 682Helical; Name=7Sequence analysisAdd BLAST22
Topological domaini683 – 764CytoplasmicSequence analysisAdd BLAST82

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Involvement in diseasei

Defects in Tshr are the cause of hyt/hyt hypothyroidism, an autosomal recessive, fetal-onset, severe hypothyroidism related to TSH hyporesponsiveness and associated with elevated TSH.1 Publication

Keywords - Diseasei

Disease mutation

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21By similarityAdd BLAST21
ChainiPRO_000001278722 – 764Thyrotropin receptorAdd BLAST743

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi31 ↔ 41PROSITE-ProRule annotation
Glycosylationi77N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi99N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi177N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi198N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi302N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei385SulfotyrosineBy similarity1
Disulfide bondi494 ↔ 569PROSITE-ProRule annotation

Post-translational modificationi

Glycosylated.By similarity
Sulfated. Sulfation on Tyr-385 plays a role in thyrotropin receptor binding and activation.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Sulfation

Proteomic databases

PaxDbiP47750
PRIDEiP47750

PTM databases

iPTMnetiP47750
PhosphoSitePlusiP47750

Expressioni

Gene expression databases

BgeeiENSMUSG00000020963 Expressed in 87 organ(s), highest expression level in brown adipose tissue
CleanExiMM_TSHR
ExpressionAtlasiP47750 baseline and differential
GenevisibleiP47750 MM

Interactioni

Subunit structurei

Interacts with heterodimer GPHA2:GPHB5; this interaction stimulates cAMP production. Interacts (via the PDZ-binding motif) with SCRIB; regulates TSHR trafficking and function.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000021346

Structurei

3D structure databases

ProteinModelPortaliP47750
SMRiP47750
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati100 – 124LRR 1Add BLAST25
Repeati125 – 150LRR 2Add BLAST26
Repeati151 – 174LRR 3Add BLAST24
Repeati176 – 199LRR 4Add BLAST24
Repeati200 – 223LRR 5Add BLAST24
Repeati225 – 248LRR 6Add BLAST24
Repeati264 – 288LRR 7Add BLAST25

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi762 – 764PDZ-binding3

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2087 Eukaryota
ENOG410XR1T LUCA
GeneTreeiENSGT00760000119088
HOGENOMiHOG000045902
HOVERGENiHBG003521
InParanoidiP47750
KOiK04249
OMAiDYTVCGG
OrthoDBiEOG091G02BV
PhylomeDBiP47750
TreeFamiTF316814

Family and domain databases

Gene3Di3.80.10.10, 1 hit
InterProiView protein in InterPro
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
IPR002131 Gphrmn_rcpt_fam
IPR026906 LRR_5
IPR032675 LRR_dom_sf
IPR002274 TSH_rcpt
IPR034298 TSHR/LHCGR/FSHR
PANTHERiPTHR24372 PTHR24372, 1 hit
PTHR24372:SF0 PTHR24372:SF0, 1 hit
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PF13306 LRR_5, 2 hits
PRINTSiPR00373 GLYCHORMONER
PR00237 GPCRRHODOPSN
PR01145 TSHRECEPTOR
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

P47750-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRPGSLLLLV LLLALSRSLR GKECASPPCE CHQEDDFRVT CKELHRIPSL
60 70 80 90 100
PPSTQTLKLI ETHLKTIPSL AFSSLPNISR IYLSIDATLQ RLEPHSFYNL
110 120 130 140 150
SKMTHIEIRN TRSLTYIDPD ALTELPLLKF LGIFNTGLRI FPDLTKIYST
160 170 180 190 200
DIFFILEITD NPYMTSVPEN AFQGLCNETL TLKLYNNGFT SVQGHAFNGT
210 220 230 240 250
KLDAVYLNKN KYLTAIDNDA FGGVYSGPTL LDVSSTSVTA LPSKGLEHLK
260 270 280 290 300
ELIAKDTWTL KKLPLSLSFL HLTRADLSYP SHCCAFKNQK KIRGILESLM
310 320 330 340 350
CNESSIRNLR QRKSVNILRG PIYQEYEEDP GDNSVGYKQN SKFQESPSNS
360 370 380 390 400
HYYVFFEEQE DEVVGFGQEL KNPQEETLQA FESHYDYTVC GDNEDMVCTP
410 420 430 440 450
KSDEFNPCED IMGYRFLRIV VWFVSLLALL GNIFVLLILL TSHYKLTVPR
460 470 480 490 500
FLMCNLAFAD FCMGVYLLLI ASVDLYTHSE YYNHAIDWQT GPGCNTAGFF
510 520 530 540 550
TVFASELSVY TLTVITLERW YAITFAMRLD RKIRLRHAYT IMAGGWVSCF
560 570 580 590 600
LLALLPMVGI SSYAKVSICL PMDTDTPLAL AYIVLVLLLN VVAFVVVCSC
610 620 630 640 650
YVKIYITVRN PQYNPRDKDT KIAKRMAVLI FTDFMCMAPI SFYALSALMN
660 670 680 690 700
KPLITVTNSK ILLVLFYPLN SCANPFLYAI FTKAFQRDVF ILLSKFGICK
710 720 730 740 750
RQAQAYQGQR VCPNNSTGIQ IQKIPQDTRQ SLPNMQDTYE LLGNSQLAPK
760
LQGQISEEYK QTAL
Length:764
Mass (Da):86,583
Last modified:October 1, 1996 - v2
Checksum:i6ADD2CC72F018317
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q78U67Q78U67_MOUSE
Thyrotropin receptor
Tshr
245Annotation score:
A0A087WP53A0A087WP53_MOUSE
Thyrotropin receptor
Tshr
127Annotation score:
A0A1Y7VLF3A0A1Y7VLF3_MOUSE
Thyrotropin receptor
Tshr
207Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti312R → S in BAB29408 (PubMed:16141072).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti556P → L in hypothyroidism. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02601 mRNA Translation: AAA53209.1
U02602 mRNA Translation: AAB60455.1
AK014519 mRNA Translation: BAB29408.1
AK029084 mRNA Translation: BAC26286.1
BC086691 mRNA Translation: AAH86691.1
BC092523 mRNA Translation: AAH92523.1
CCDSiCCDS26088.1
PIRiI48882
RefSeqiNP_035778.3, NM_011648.5
UniGeneiMm.173847

Genome annotation databases

EnsembliENSMUST00000021346; ENSMUSP00000021346; ENSMUSG00000020963
GeneIDi22095
KEGGimmu:22095
UCSCiuc007okq.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02601 mRNA Translation: AAA53209.1
U02602 mRNA Translation: AAB60455.1
AK014519 mRNA Translation: BAB29408.1
AK029084 mRNA Translation: BAC26286.1
BC086691 mRNA Translation: AAH86691.1
BC092523 mRNA Translation: AAH92523.1
CCDSiCCDS26088.1
PIRiI48882
RefSeqiNP_035778.3, NM_011648.5
UniGeneiMm.173847

3D structure databases

ProteinModelPortaliP47750
SMRiP47750
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000021346

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP47750
PhosphoSitePlusiP47750

Proteomic databases

PaxDbiP47750
PRIDEiP47750

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021346; ENSMUSP00000021346; ENSMUSG00000020963
GeneIDi22095
KEGGimmu:22095
UCSCiuc007okq.2 mouse

Organism-specific databases

CTDi7253
MGIiMGI:98849 Tshr

Phylogenomic databases

eggNOGiKOG2087 Eukaryota
ENOG410XR1T LUCA
GeneTreeiENSGT00760000119088
HOGENOMiHOG000045902
HOVERGENiHBG003521
InParanoidiP47750
KOiK04249
OMAiDYTVCGG
OrthoDBiEOG091G02BV
PhylomeDBiP47750
TreeFamiTF316814

Enzyme and pathway databases

ReactomeiR-MMU-375281 Hormone ligand-binding receptors
R-MMU-418555 G alpha (s) signalling events

Miscellaneous databases

PROiPR:P47750
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020963 Expressed in 87 organ(s), highest expression level in brown adipose tissue
CleanExiMM_TSHR
ExpressionAtlasiP47750 baseline and differential
GenevisibleiP47750 MM

Family and domain databases

Gene3Di3.80.10.10, 1 hit
InterProiView protein in InterPro
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
IPR002131 Gphrmn_rcpt_fam
IPR026906 LRR_5
IPR032675 LRR_dom_sf
IPR002274 TSH_rcpt
IPR034298 TSHR/LHCGR/FSHR
PANTHERiPTHR24372 PTHR24372, 1 hit
PTHR24372:SF0 PTHR24372:SF0, 1 hit
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PF13306 LRR_5, 2 hits
PRINTSiPR00373 GLYCHORMONER
PR00237 GPCRRHODOPSN
PR01145 TSHRECEPTOR
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTSHR_MOUSE
AccessioniPrimary (citable) accession number: P47750
Secondary accession number(s): Q562E4, Q9D697
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 1, 1996
Last modified: November 7, 2018
This is version 162 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again