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UniProtKB - P47743 (GRM8_MOUSE)
Protein
Metabotropic glutamate receptor 8
Gene
Grm8
Organism
Mus musculus (Mouse)
Status
Functioni
G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 156 | GlutamateBy similarity | 1 | |
Binding sitei | 227 | GlutamateBy similarity | 1 | |
Binding sitei | 309 | GlutamateBy similarity | 1 | |
Binding sitei | 401 | GlutamateBy similarity | 1 |
GO - Molecular functioni
- adenylate cyclase inhibiting G protein-coupled glutamate receptor activity Source: GO_Central
- glutamate receptor activity Source: UniProtKB
- G protein-coupled receptor activity Source: UniProtKB
- group III metabotropic glutamate receptor activity Source: MGI
GO - Biological processi
- adenylate cyclase-inhibiting G protein-coupled glutamate receptor signaling pathway Source: UniProtKB
- adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway Source: MGI
- chemical synaptic transmission Source: MGI
- detection of mechanical stimulus involved in sensory perception of pain Source: MGI
- G protein-coupled glutamate receptor signaling pathway Source: GO_Central
- regulation of neurotransmitter secretion Source: MGI
- regulation of synaptic transmission, glutamatergic Source: GO_Central
- regulation of synaptic vesicle exocytosis Source: MGI
- synaptic transmission, glutamatergic Source: MGI
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Biological process | Sensory transduction |
Enzyme and pathway databases
Reactomei | R-MMU-418594, G alpha (i) signalling events R-MMU-420499, Class C/3 (Metabotropic glutamate/pheromone receptors) |
Names & Taxonomyi
Protein namesi | Recommended name: Metabotropic glutamate receptor 8Short name: mGluR8 |
Gene namesi | Name:Grm8 Synonyms:Gprc1h, Mglur8 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1351345, Grm8 |
VEuPathDBi | HostDB:ENSMUSG00000024211 |
Subcellular locationi
Plasma membrane
Plasma Membrane
- integral component of plasma membrane Source: MGI
- integral component of presynaptic active zone membrane Source: MGI
- presynaptic membrane Source: MGI
Other locations
- GABA-ergic synapse Source: MGI
- glutamatergic synapse Source: MGI
- neuron projection Source: MGI
- neuronal cell body Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 34 – 583 | ExtracellularSequence analysisAdd BLAST | 550 | |
Transmembranei | 584 – 608 | Helical; Name=1Sequence analysisAdd BLAST | 25 | |
Topological domaini | 609 – 620 | CytoplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 621 – 641 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 642 – 647 | ExtracellularSequence analysis | 6 | |
Transmembranei | 648 – 668 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 669 – 695 | CytoplasmicSequence analysisAdd BLAST | 27 | |
Transmembranei | 696 – 716 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Topological domaini | 717 – 746 | ExtracellularSequence analysisAdd BLAST | 30 | |
Transmembranei | 747 – 768 | Helical; Name=5Sequence analysisAdd BLAST | 22 | |
Topological domaini | 769 – 781 | CytoplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 782 – 803 | Helical; Name=6Sequence analysisAdd BLAST | 22 | |
Topological domaini | 804 – 818 | ExtracellularSequence analysisAdd BLAST | 15 | |
Transmembranei | 819 – 843 | Helical; Name=7Sequence analysisAdd BLAST | 25 | |
Topological domaini | 844 – 908 | CytoplasmicSequence analysisAdd BLAST | 65 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 868 | K → R: No change in sumoylation. Abolishes sumoylation; when associated with R-872 and R-882. 1 Publication | 1 | |
Mutagenesisi | 872 | K → R: No change in sumoylation. Abolishes sumoylation; when associated with R-868 and R-882. 1 Publication | 1 | |
Mutagenesisi | 882 | K → R: Abolishes sumoylation. Abolishes sumoylation; when associated with R-868 and R-872. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL4626 |
DrugCentrali | P47743 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 33 | Sequence analysisAdd BLAST | 33 | |
ChainiPRO_0000012942 | 34 – 908 | Metabotropic glutamate receptor 8Add BLAST | 875 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 64 ↔ 106 | By similarity | ||
Glycosylationi | 95 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 246 ↔ 534 | By similarity | ||
Glycosylationi | 298 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 369 ↔ 384 | By similarity | ||
Disulfide bondi | 424 ↔ 431 | By similarity | ||
Glycosylationi | 452 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 480 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 516 ↔ 535 | By similarity | ||
Disulfide bondi | 520 ↔ 538 | By similarity | ||
Disulfide bondi | 541 ↔ 553 | By similarity | ||
Disulfide bondi | 556 ↔ 569 | By similarity | ||
Glycosylationi | 565 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Cross-linki | 882 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)1 Publication |
Keywords - PTMi
Disulfide bond, Glycoprotein, Isopeptide bond, Ubl conjugationProteomic databases
PaxDbi | P47743 |
PRIDEi | P47743 |
ProteomicsDBi | 269638 |
PTM databases
GlyGeni | P47743, 5 sites |
iPTMneti | P47743 |
PhosphoSitePlusi | P47743 |
Expressioni
Tissue specificityi
Strongly expressed in olfactory bulb, accessory olfactory bulb, and mammillary body. Weaker expression in the retina, and in scattered cells in the cortex and hindbrain.
Gene expression databases
Bgeei | ENSMUSG00000024211, Expressed in secondary oocyte and 121 other tissues |
ExpressionAtlasi | P47743, baseline and differential |
Genevisiblei | P47743, MM |
Interactioni
Subunit structurei
Interacts with PICK1.
By similarityProtein-protein interaction databases
BioGRIDi | 200074, 5 interactors |
IntActi | P47743, 1 interactor |
MINTi | P47743 |
STRINGi | 10090.ENSMUSP00000110978 |
Chemistry databases
BindingDBi | P47743 |
Miscellaneous databases
RNActi | P47743, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 177 – 179 | Glutamate bindingBy similarity | 3 |
Sequence similaritiesi
Belongs to the G-protein coupled receptor 3 family.Curated
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1056, Eukaryota |
GeneTreei | ENSGT01030000234648 |
HOGENOMi | CLU_005389_0_0_1 |
InParanoidi | P47743 |
OrthoDBi | 483746at2759 |
PhylomeDBi | P47743 |
Family and domain databases
Gene3Di | 2.10.50.30, 1 hit |
InterProi | View protein in InterPro IPR001828, ANF_lig-bd_rcpt IPR000337, GPCR_3 IPR011500, GPCR_3_9-Cys_dom IPR038550, GPCR_3_9-Cys_sf IPR017978, GPCR_3_C IPR017979, GPCR_3_CS IPR000162, GPCR_3_mtglu_rcpt IPR000144, GPCR_3_mtglu_rcpt_8 IPR028082, Peripla_BP_I |
Pfami | View protein in Pfam PF00003, 7tm_3, 1 hit PF01094, ANF_receptor, 1 hit PF07562, NCD3G, 1 hit |
PRINTSi | PR00248, GPCRMGR PR01058, MTABOTROPC8R PR00593, MTABOTROPICR |
SUPFAMi | SSF53822, SSF53822, 1 hit |
PROSITEi | View protein in PROSITE PS00979, G_PROTEIN_RECEP_F3_1, 1 hit PS00980, G_PROTEIN_RECEP_F3_2, 1 hit PS00981, G_PROTEIN_RECEP_F3_3, 1 hit PS50259, G_PROTEIN_RECEP_F3_4, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
P47743-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVCEGKRSTS CPCFFLLTAK FYWILTMMQR THSQEYAHSI RLDGDIILGG
60 70 80 90 100
LFPVHAKGER GVPCGDLKKE KGIHRLEAML YAIDQINKDP DLLSNITLGV
110 120 130 140 150
RILDTCSRDT YALEQSLTFV QALIEKDASD VKCANGDPPI FTKPDKISGV
160 170 180 190 200
IGAAASSVSI MVANILRLFK IPQISYASTA PELSDNTRYD FFSRVVPPDS
210 220 230 240 250
YQAQAMVDIV TALGWNYVST LASEGNYGES GVEAFTQISR EIGGVCIAQS
260 270 280 290 300
QKIPREPRPG EFEKIIKRLL ETPNARAVIM FANEDDIRRI LEAAKKLNQS
310 320 330 340 350
GHFLWIGSDS WGSKIAPVYQ QEEIAEGAVT ILPKRASIDG FDRYFRSRTL
360 370 380 390 400
ANNRRNVWFA EFWEENFGCK LGSHGKRNSH IKKCTGLERI ARDSSYEQEG
410 420 430 440 450
KVQFVIDAVY SMAYALHNMH KELCPGYIGL CPRMVTIDGK ELLGYIRAVN
460 470 480 490 500
FNGSAGTPVT FNENGDAPGR YDIFQYQINN KSTEYKIIGH WTNQLHLKVE
510 520 530 540 550
DMQWANREHT HPASVCSLPC KPGERKKTVK GVPCCWHCER CEGYNYQVDE
560 570 580 590 600
LSCELCPLDQ RPNINRTGCQ RIPIIKLEWH SPWAVVPVFI AILGIIATTF
610 620 630 640 650
VIVTFVRYND TPIVRASGRE LSYVLLTGIF LCYSITFLMI AAPDTIICSF
660 670 680 690 700
RRIFLGLGMC FSYAALLTKT NRIHRIFEQG KKSVTAPKFI SPASQLVITF
710 720 730 740 750
SLISVQLLGV FVWFVVDPPH TIIDYGEQRT LDPENARGVL KCDISDLSLI
760 770 780 790 800
CSLGYSILLM VTCTVYAIKT RGVPETFNEA KPIGFTMYTT CIIWLAFIPI
810 820 830 840 850
FFGTAQSAEK MYIQTTTLTV SMSLSASVSL GMLYMPKVYI IIFHPEQNVQ
860 870 880 890 900
KRKRSFKAVV TAATMQSKLI QKGNDRPNGE VKSELCESLE TNTSSTKTTY
ISYSNHSI
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ05BD6 | Q05BD6_MOUSE | Glutamate receptor, metabotropic 8 | Grm8 | 908 | Annotation score: | ||
D3YZL1 | D3YZL1_MOUSE | Metabotropic glutamate receptor 8 | Grm8 | 293 | Annotation score: | ||
D3YZK8 | D3YZK8_MOUSE | Metabotropic glutamate receptor 8 | Grm8 | 20 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 86 | I → T in AAA68149 (PubMed:7722646).Curated | 1 | |
Sequence conflicti | 289 | R → G in AAA68149 (PubMed:7722646).Curated | 1 | |
Sequence conflicti | 363 – 365 | WEE → SEG in AAA68149 (PubMed:7722646).Curated | 3 | |
Sequence conflicti | 371 | L → S in AAA68149 (PubMed:7722646).Curated | 1 | |
Sequence conflicti | 539 | E → G in AAA68149 (PubMed:7722646).Curated | 1 | |
Sequence conflicti | 589 | F → L in AAA68149 (PubMed:7722646).Curated | 1 | |
Sequence conflicti | 905 | N → D in AAA68149 (PubMed:7722646).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U17252 mRNA Translation: AAA68149.1 AY673682 mRNA Translation: AAT76980.1 |
CCDSi | CCDS80495.1 |
PIRi | I49142 |
RefSeqi | NP_001298001.1, NM_001311072.1 XP_017176886.1, XM_017321397.1 |
Genome annotation databases
Ensembli | ENSMUST00000090512; ENSMUSP00000087998; ENSMUSG00000024211 |
GeneIDi | 14823 |
KEGGi | mmu:14823 |
UCSCi | uc009bcj.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U17252 mRNA Translation: AAA68149.1 AY673682 mRNA Translation: AAT76980.1 |
CCDSi | CCDS80495.1 |
PIRi | I49142 |
RefSeqi | NP_001298001.1, NM_001311072.1 XP_017176886.1, XM_017321397.1 |
3D structure databases
SMRi | P47743 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 200074, 5 interactors |
IntActi | P47743, 1 interactor |
MINTi | P47743 |
STRINGi | 10090.ENSMUSP00000110978 |
Chemistry databases
BindingDBi | P47743 |
ChEMBLi | CHEMBL4626 |
DrugCentrali | P47743 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | P47743, 5 sites |
iPTMneti | P47743 |
PhosphoSitePlusi | P47743 |
Proteomic databases
PaxDbi | P47743 |
PRIDEi | P47743 |
ProteomicsDBi | 269638 |
Protocols and materials databases
Antibodypediai | 17706, 376 antibodies from 34 providers |
DNASUi | 14823 |
Genome annotation databases
Ensembli | ENSMUST00000090512; ENSMUSP00000087998; ENSMUSG00000024211 |
GeneIDi | 14823 |
KEGGi | mmu:14823 |
UCSCi | uc009bcj.1, mouse |
Organism-specific databases
CTDi | 2918 |
MGIi | MGI:1351345, Grm8 |
VEuPathDBi | HostDB:ENSMUSG00000024211 |
Phylogenomic databases
eggNOGi | KOG1056, Eukaryota |
GeneTreei | ENSGT01030000234648 |
HOGENOMi | CLU_005389_0_0_1 |
InParanoidi | P47743 |
OrthoDBi | 483746at2759 |
PhylomeDBi | P47743 |
Enzyme and pathway databases
Reactomei | R-MMU-418594, G alpha (i) signalling events R-MMU-420499, Class C/3 (Metabotropic glutamate/pheromone receptors) |
Miscellaneous databases
BioGRID-ORCSi | 14823, 1 hit in 64 CRISPR screens |
ChiTaRSi | Grm8, mouse |
PROi | PR:P47743 |
RNActi | P47743, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024211, Expressed in secondary oocyte and 121 other tissues |
ExpressionAtlasi | P47743, baseline and differential |
Genevisiblei | P47743, MM |
Family and domain databases
Gene3Di | 2.10.50.30, 1 hit |
InterProi | View protein in InterPro IPR001828, ANF_lig-bd_rcpt IPR000337, GPCR_3 IPR011500, GPCR_3_9-Cys_dom IPR038550, GPCR_3_9-Cys_sf IPR017978, GPCR_3_C IPR017979, GPCR_3_CS IPR000162, GPCR_3_mtglu_rcpt IPR000144, GPCR_3_mtglu_rcpt_8 IPR028082, Peripla_BP_I |
Pfami | View protein in Pfam PF00003, 7tm_3, 1 hit PF01094, ANF_receptor, 1 hit PF07562, NCD3G, 1 hit |
PRINTSi | PR00248, GPCRMGR PR01058, MTABOTROPC8R PR00593, MTABOTROPICR |
SUPFAMi | SSF53822, SSF53822, 1 hit |
PROSITEi | View protein in PROSITE PS00979, G_PROTEIN_RECEP_F3_1, 1 hit PS00980, G_PROTEIN_RECEP_F3_2, 1 hit PS00981, G_PROTEIN_RECEP_F3_3, 1 hit PS50259, G_PROTEIN_RECEP_F3_4, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GRM8_MOUSE | |
Accessioni | P47743Primary (citable) accession number: P47743 Secondary accession number(s): Q6B964 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1996 |
Last sequence update: | September 27, 2005 | |
Last modified: | February 23, 2022 | |
This is version 169 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families