UniProtKB - P47740 (AL3A2_MOUSE)
Protein
Aldehyde dehydrogenase family 3 member A2
Gene
Aldh3a2
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the oxidation of medium and long-chain aliphatic aldehydes to fatty acids. Active on a variety of saturated and unsaturated aliphatic aldehydes between 6 and 24 carbons in length (PubMed:25286108). Responsible for conversion of the sphingosine 1-phosphate (S1P) degradation product hexadecenal to hexadecenoic acid (PubMed:25286108).1 Publication
Catalytic activityi
- EC:1.2.1.31 Publication
- 2,6,10,14-tetramethylpentadecanal + H2O + NAD+ = 2,6,10,14-tetramethylpentadecanoate + 2 H+ + NADHBy similarity
- EC:1.2.1.94By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 207 | PROSITE-ProRule annotation | 1 | |
Active sitei | 241 | PROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 185 – 190 | NADSequence analysis | 6 |
GO - Molecular functioni
- 3-chloroallyl aldehyde dehydrogenase activity Source: MGI
- aldehyde dehydrogenase (NAD+) activity Source: MGI
- glyceraldehyde-3-phosphate dehydrogenase (NAD+) (non-phosphorylating) activity Source: UniProtKB-EC
- long-chain-alcohol oxidase activity Source: MGI
- long-chain-aldehyde dehydrogenase activity Source: UniProtKB
- medium-chain-aldehyde dehydrogenase activity Source: UniProtKB
- protein homodimerization activity Source: MGI
GO - Biological processi
- cellular aldehyde metabolic process Source: MGI
- central nervous system development Source: MGI
- epidermis development Source: MGI
- fatty acid metabolic process Source: UniProtKB-KW
- formaldehyde metabolic process Source: MGI
- hexadecanal metabolic process Source: UniProtKB
- oxidation-reduction process Source: MGI
- peripheral nervous system development Source: MGI
- phytol metabolic process Source: MGI
- response to reactive oxygen species Source: MGI
- sesquiterpenoid metabolic process Source: MGI
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Fatty acid metabolism, Lipid metabolism |
Ligand | NAD |
Enzyme and pathway databases
BRENDAi | 1.2.1.3, 3474 |
Reactomei | R-MMU-1660661, Sphingolipid de novo biosynthesis R-MMU-389599, Alpha-oxidation of phytanate R-MMU-9603798, Class I peroxisomal membrane protein import R-MMU-9609523, Insertion of tail-anchored proteins into the endoplasmic reticulum membrane |
Chemistry databases
SwissLipidsi | SLP:000001741 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Aldh3a2 Synonyms:Ahd-3, Ahd3, Aldh3, Aldh4 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1353452, Aldh3a2 |
Subcellular locationi
Endoplasmic reticulum
- Microsome membrane By similarity; Single-pass membrane protein By similarity
- Endoplasmic reticulum membrane By similarity; Single-pass membrane protein By similarity; Cytoplasmic side By similarity
Other locations
- Membrane 1 Publication
Cytosol
- cytosol Source: MGI
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
- extrinsic component of endoplasmic reticulum membrane Source: MGI
Mitochondrion
- mitochondrial inner membrane Source: MGI
- mitochondrion Source: MGI
Nucleus
- nucleus Source: MGI
Peroxisome
- peroxisome Source: MGI
Other locations
- integral component of membrane Source: MGI
- intracellular membrane-bounded organelle Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 463 | CytoplasmicAdd BLAST | 463 | |
Transmembranei | 464 – 484 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, MicrosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056475 | 1 – 484 | Aldehyde dehydrogenase family 3 member A2Add BLAST | 484 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 293 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | P47740 |
jPOSTi | P47740 |
MaxQBi | P47740 |
PaxDbi | P47740 |
PRIDEi | P47740 |
PTM databases
iPTMneti | P47740 |
PhosphoSitePlusi | P47740 |
SwissPalmi | P47740 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000010025, Expressed in prostate gland ventral lobe and 301 other tissues |
ExpressionAtlasi | P47740, baseline and differential |
Genevisiblei | P47740, MM |
Interactioni
Subunit structurei
Homodimer.
By similarityGO - Molecular functioni
- protein homodimerization activity Source: MGI
Protein-protein interaction databases
BioGRIDi | 198066, 3 interactors |
IntActi | P47740, 4 interactors |
MINTi | P47740 |
STRINGi | 10090.ENSMUSP00000073764 |
Miscellaneous databases
RNActi | P47740, protein |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 481 – 484 | Prevents secretion from ERBy similarity | 4 |
Sequence similaritiesi
Belongs to the aldehyde dehydrogenase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2456, Eukaryota |
GeneTreei | ENSGT00940000157944 |
HOGENOMi | CLU_005391_3_0_1 |
InParanoidi | P47740 |
OMAi | RHGKRWM |
OrthoDBi | 646662at2759 |
TreeFami | TF314264 |
Family and domain databases
Gene3Di | 3.40.309.10, 1 hit 3.40.605.10, 1 hit |
InterProi | View protein in InterPro IPR016161, Ald_DH/histidinol_DH IPR016163, Ald_DH_C IPR016160, Ald_DH_CS_CYS IPR029510, Ald_DH_CS_GLU IPR016162, Ald_DH_N IPR015590, Aldehyde_DH_dom IPR012394, Aldehyde_DH_NAD(P) |
Pfami | View protein in Pfam PF00171, Aldedh, 1 hit |
PIRSFi | PIRSF036492, ALDH, 1 hit |
SUPFAMi | SSF53720, SSF53720, 1 hit |
PROSITEi | View protein in PROSITE PS00070, ALDEHYDE_DEHYDR_CYS, 1 hit PS00687, ALDEHYDE_DEHYDR_GLU, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
P47740-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MERQVLRLRQ AFRSGRSRPL RFRLQQLEAL RRMVQEREKE ILAAIAADLS
60 70 80 90 100
KSELNAYSHE VITILGEIDF MLGNLPELAS ARPAKKNLLT MMDEAYVQPE
110 120 130 140 150
PLGVVLIIGA WNYPFVLTMQ PLVGAIAAGN AAIVKPSELS ENTAKILAEL
160 170 180 190 200
LPQYLDQDLY AIVNGGIPET TELLKQRFDH ILYTGNTAVG KIVMEAAAKH
210 220 230 240 250
LTPVTLELGG KSPCYIDRDC DLDVACRRIA WGKYMNCGQT CIAPDYILCE
260 270 280 290 300
ASLQNQIVQK IKETVKDFYG ENIKASPDYE RIINLRHFKR LQSLLKGQKI
310 320 330 340 350
AFGGEMDEAT RYLAPTILTD VDPNSKVMQE EIFGPILPIV SVKNVDEAIN
360 370 380 390 400
FINDREKPLA LYVFSRNNKL IKRVIDETSS GGVTGNDVIM HFTVNSLPFG
410 420 430 440 450
GVGASGMGAY HGKYSFDTFS HQRPCLLKGL KGESVNKLRY PPNSESKVSW
460 470 480
AKFFLLKQFN KGRLGMLLFV CLVAVAAVIV KDQL
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB1AV77 | B1AV77_MOUSE | Aldehyde dehydrogenase | Aldh3a2 | 487 | Annotation score: | ||
B1ATI0 | B1ATI0_MOUSE | Aldehyde dehydrogenase | Aldh3a2 | 532 | Annotation score: | ||
A0A140LJF9 | A0A140LJF9_MOUSE | Aldehyde dehydrogenase family 3 mem... | Aldh3a2 | 128 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 19 | P → L in AAB06232 (PubMed:8634152).Curated | 1 | |
Sequence conflicti | 229 | I → V in AAB06232 (PubMed:8634152).Curated | 1 | |
Sequence conflicti | 418 | T → A in AAB06232 (PubMed:8634152).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14390 mRNA Translation: AAB06232.1 AK079639 mRNA Translation: BAC37712.1 AK140932 mRNA Translation: BAE24523.1 AK159246 mRNA Translation: BAE34928.1 AK163040 mRNA Translation: BAE37166.1 AK169157 mRNA Translation: BAE40936.1 AK170195 mRNA Translation: BAE41628.1 AL672172 Genomic DNA No translation available. BC003797 mRNA Translation: AAH03797.1 |
CCDSi | CCDS24809.1 |
RefSeqi | NP_031463.2, NM_007437.5 |
Genome annotation databases
Ensembli | ENSMUST00000074127; ENSMUSP00000073764; ENSMUSG00000010025 |
GeneIDi | 11671 |
KEGGi | mmu:11671 |
UCSCi | uc007jhf.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14390 mRNA Translation: AAB06232.1 AK079639 mRNA Translation: BAC37712.1 AK140932 mRNA Translation: BAE24523.1 AK159246 mRNA Translation: BAE34928.1 AK163040 mRNA Translation: BAE37166.1 AK169157 mRNA Translation: BAE40936.1 AK170195 mRNA Translation: BAE41628.1 AL672172 Genomic DNA No translation available. BC003797 mRNA Translation: AAH03797.1 |
CCDSi | CCDS24809.1 |
RefSeqi | NP_031463.2, NM_007437.5 |
3D structure databases
SMRi | P47740 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 198066, 3 interactors |
IntActi | P47740, 4 interactors |
MINTi | P47740 |
STRINGi | 10090.ENSMUSP00000073764 |
Chemistry databases
SwissLipidsi | SLP:000001741 |
PTM databases
iPTMneti | P47740 |
PhosphoSitePlusi | P47740 |
SwissPalmi | P47740 |
Proteomic databases
EPDi | P47740 |
jPOSTi | P47740 |
MaxQBi | P47740 |
PaxDbi | P47740 |
PRIDEi | P47740 |
Protocols and materials databases
Antibodypediai | 2232, 442 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000074127; ENSMUSP00000073764; ENSMUSG00000010025 |
GeneIDi | 11671 |
KEGGi | mmu:11671 |
UCSCi | uc007jhf.2, mouse |
Organism-specific databases
CTDi | 224 |
MGIi | MGI:1353452, Aldh3a2 |
Phylogenomic databases
eggNOGi | KOG2456, Eukaryota |
GeneTreei | ENSGT00940000157944 |
HOGENOMi | CLU_005391_3_0_1 |
InParanoidi | P47740 |
OMAi | RHGKRWM |
OrthoDBi | 646662at2759 |
TreeFami | TF314264 |
Enzyme and pathway databases
BRENDAi | 1.2.1.3, 3474 |
Reactomei | R-MMU-1660661, Sphingolipid de novo biosynthesis R-MMU-389599, Alpha-oxidation of phytanate R-MMU-9603798, Class I peroxisomal membrane protein import R-MMU-9609523, Insertion of tail-anchored proteins into the endoplasmic reticulum membrane |
Miscellaneous databases
BioGRID-ORCSi | 11671, 0 hits in 17 CRISPR screens |
ChiTaRSi | Aldh3a2, mouse |
PROi | PR:P47740 |
RNActi | P47740, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000010025, Expressed in prostate gland ventral lobe and 301 other tissues |
ExpressionAtlasi | P47740, baseline and differential |
Genevisiblei | P47740, MM |
Family and domain databases
Gene3Di | 3.40.309.10, 1 hit 3.40.605.10, 1 hit |
InterProi | View protein in InterPro IPR016161, Ald_DH/histidinol_DH IPR016163, Ald_DH_C IPR016160, Ald_DH_CS_CYS IPR029510, Ald_DH_CS_GLU IPR016162, Ald_DH_N IPR015590, Aldehyde_DH_dom IPR012394, Aldehyde_DH_NAD(P) |
Pfami | View protein in Pfam PF00171, Aldedh, 1 hit |
PIRSFi | PIRSF036492, ALDH, 1 hit |
SUPFAMi | SSF53720, SSF53720, 1 hit |
PROSITEi | View protein in PROSITE PS00070, ALDEHYDE_DEHYDR_CYS, 1 hit PS00687, ALDEHYDE_DEHYDR_GLU, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AL3A2_MOUSE | |
Accessioni | P47740Primary (citable) accession number: P47740 Secondary accession number(s): Q99L64 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1996 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 163 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families