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Protein

Dihydrofolate reductase

Gene

folA

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.
Proteins known to be involved in this subpathway in this organism are:
  1. Dihydrofolate reductase (folA)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei27SubstrateBy similarity1
Binding sitei57SubstrateBy similarity1
Binding sitei120SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi6 – 7NADPBy similarity2
Nucleotide bindingi14 – 19NADPBy similarity6
Nucleotide bindingi43 – 46NADPBy similarity4
Nucleotide bindingi62 – 65NADPBy similarity4
Nucleotide bindingi101 – 106NADPBy similarity6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processOne-carbon metabolism
LigandNADP

Enzyme and pathway databases

BioCyciMGEN243273:G1GJ2-275-MONOMER
UniPathwayiUPA00077; UER00158

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase (EC:1.5.1.3)
Gene namesi
Name:folA
Synonyms:dhfR
Ordered Locus Names:MG228
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000000807 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863951 – 160Dihydrofolate reductaseAdd BLAST160

Interactioni

Protein-protein interaction databases

STRINGi243273.MgenG_010200001567

Structurei

3D structure databases

ProteinModelPortaliP47470
SMRiP47470
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 160DHFRPROSITE-ProRule annotationAdd BLAST160

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni5 – 7Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated

Phylogenomic databases

eggNOGiENOG41084DH Bacteria
COG0262 LUCA
KOiK00287
OMAiEECFVIG
OrthoDBiPOG091H01KY

Family and domain databases

CDDicd00209 DHFR, 1 hit
Gene3Di3.40.430.10, 1 hit
InterProiView protein in InterPro
IPR012259 DHFR
IPR024072 DHFR-like_dom_sf
IPR017925 DHFR_CS
IPR001796 DHFR_dom
PANTHERiPTHR22778:SF16 PTHR22778:SF16, 1 hit
PfamiView protein in Pfam
PF00186 DHFR_1, 1 hit
SUPFAMiSSF53597 SSF53597, 1 hit
PROSITEiView protein in PROSITE
PS00075 DHFR_1, 1 hit
PS51330 DHFR_2, 1 hit

Sequencei

Sequence statusi: Complete.

P47470-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIAIWAMTQ EGLIGNNNTL PWMIKQELAH FKKTTLFQAL LMGRKTYESL
60 70 80 90 100
PKVFEKRTIF LLSKDQNYRF EEKGSEVKVI NDFWPLIKSY QANKEKDLFI
110 120 130 140 150
CGGKSVYEQT INECDQLIVS IIKKKYKGDQ FLKVDLSKFV LNEVVEFEEF
160
NVNYYRKKQQ
Length:160
Mass (Da):18,966
Last modified:February 1, 1996 - v1
Checksum:iBE46EBAE24DDBE13
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA Translation: AAC71449.1
PIRiB64225
RefSeqiWP_009885765.1, NZ_AAGX01000005.1

Genome annotation databases

EnsemblBacteriaiAAC71449; AAC71449; MG_228
KEGGimge:MG_228

Similar proteinsi

Entry informationi

Entry nameiDYR_MYCGE
AccessioniPrimary (citable) accession number: P47470
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: March 28, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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