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Protein

UvrABC system protein B

Gene

uvrB

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi42 – 49ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionExcision nuclease
Biological processDNA damage, DNA excision, DNA repair, SOS response
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMGEN243273:G1GJ2-85-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
UvrABC system protein BUniRule annotation
Short name:
Protein UvrBUniRule annotation
Alternative name(s):
Excinuclease ABC subunit BUniRule annotation
Gene namesi
Name:uvrBUniRule annotation
Ordered Locus Names:MG073
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000000807 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001384071 – 656UvrABC system protein BAdd BLAST656

Proteomic databases

PRIDEiP47319

Interactioni

Subunit structurei

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP47319
SMRiP47319
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 414Helicase ATP-bindingUniRule annotationAdd BLAST386
Domaini434 – 596Helicase C-terminalUniRule annotationAdd BLAST163
Domaini614 – 649UVRUniRule annotationAdd BLAST36

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi95 – 118Beta-hairpinAdd BLAST24

Domaini

The beta-hairpin motif is involved in DNA binding.UniRule annotation

Sequence similaritiesi

Belongs to the UvrB family.UniRule annotation

Phylogenomic databases

KOiK03702
OMAiRYMHSEI
OrthoDBiPOG091H02LK

Family and domain databases

CDDicd00079 HELICc, 1 hit
HAMAPiMF_00204 UvrB, 1 hit
InterProiView protein in InterPro
IPR006935 Helicase/UvrB_N
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR001943 UVR_dom
IPR036876 UVR_dom_sf
IPR004807 UvrB
IPR024759 UvrB_YAD/RRR_dom
PANTHERiPTHR24029 PTHR24029, 1 hit
PfamiView protein in Pfam
PF00271 Helicase_C, 1 hit
PF04851 ResIII, 1 hit
PF02151 UVR, 1 hit
PF12344 UvrB, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF46600 SSF46600, 1 hit
SSF52540 SSF52540, 3 hits
TIGRFAMsiTIGR00631 uvrb, 1 hit
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS50151 UVR, 1 hit

Sequencei

Sequence statusi: Complete.

P47319-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNQTKSNSL FQLSTNYIPT GDQPEAIKKL SEFKTKQQVL LGATGTGKTF
60 70 80 90 100
TIANVIQNSQ LPTVVIAHNK TLAGQLFNEL KQLFPKNAVE YFISYFDFYQ
110 120 130 140 150
PEAYLPSKGI YIEKSATVNE AIKRLRVSTL HSLSTRKDVI VVGSVASIYP
160 170 180 190 200
TSSPSDFVKY CLWFVVGKDY DLKTIKDRLV SLNYVVNKQQ LTPGKFRFQG
210 220 230 240 250
DVLEVFPGYS DAFVIRISFF DTKVEQICQI DPLTNKILNQ LFEIKIGPAD
260 270 280 290 300
EYVVNQSDLD IAIKNIKQEL QERVNYFNKQ NLVERAQRLA TITNHDLNDL
310 320 330 340 350
KAWGFCSGVE NYARHLELRM ANSTPYSIFD YFKGDWLLVI DESHQTLPQL
360 370 380 390 400
NGMYNTDLSR KQSLIDYGFR LPSALDNRPL SFAELQQKMQ KVIYVSATPR
410 420 430 440 450
DKEISLSQNN VIEQLVRPTY LVDPIIVVKP KDNQVEDLIE EIINQRQNNT
460 470 480 490 500
RTFVTVLTIK MAENLTEYLK ERKIKVAYIH KDIKALERLL LINDLRRGEY
510 520 530 540 550
ECLVGINLLR EGLDVPEVAL VCIFDADIPG LPRDERSLIQ IIGRAARNEH
560 570 580 590 600
GRVVMYANHV TEQMQKAIDE TKRRRTVQME YNKLHNKTPK TVVKPLTFVQ
610 620 630 640 650
PIKLKAKSNA EKNAALIKQL TKEMKKAAAN QNYELAIEIR DSIFELEKEI

GSKIKV
Length:656
Mass (Da):75,202
Last modified:February 1, 1996 - v1
Checksum:i59488ADF5D283493
GO

Sequence cautioni

The sequence AAD10554 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti45G → A (PubMed:8253680).Curated1
Sequence conflicti102 – 107EAYLPS → KLTYPD in AAD12393 (PubMed:8253680).Curated6
Sequence conflicti432 – 433DN → IT (PubMed:8253680).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA Translation: AAC71291.1
U01743 Genomic DNA Translation: AAD10554.1 Different initiation.
U02119 Genomic DNA Translation: AAD12393.1
U01698 Genomic DNA Translation: AAB01010.1
PIRiA64208
RefSeqiWP_010869318.1, NC_000908.2

Genome annotation databases

EnsemblBacteriaiAAC71291; AAC71291; MG_073
KEGGimge:MG_073

Similar proteinsi

Entry informationi

Entry nameiUVRB_MYCGE
AccessioniPrimary (citable) accession number: P47319
Secondary accession number(s): Q49254, Q49446, Q49506
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 23, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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