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Protein

CAAX prenyl protease 1

Gene

STE24

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Proteolytically removes the C-terminal three residues of farnesylated A-factor mating pheromone. Also acts to cleave the N-terminal extension of the pheromone. Does not act on Ras.5 Publications

Miscellaneous

Present with 19600 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

The peptide bond hydrolyzed can be designated -C-|-A-A-X in which C is an S-isoprenylated cysteine residue, A is usually aliphatic and X is the C-terminal residue of the substrate protein, and may be any of several amino acids.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi297Zinc; catalyticPROSITE-ProRule annotation1
Active sitei298PROSITE-ProRule annotation1
Metal bindingi301Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi390Zinc; catalyticPROSITE-ProRule annotation1
Active sitei394Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • metalloendopeptidase activity Source: SGD

GO - Biological processi

  • CAAX-box protein processing Source: SGD
  • peptide mating pheromone maturation involved in posititve regulation of conjugation with cellular fusion Source: SGD
  • protein processing Source: SGD
  • response to pheromone Source: UniProtKB-KW

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
Biological processPheromone response
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:YJR117W-MONOMER
YEAST:YJR117W-MONOMER
BRENDAi3.4.24.84 984

Protein family/group databases

MEROPSiM48.001
TCDBi9.B.1.1.3 the integral membrane caax protease (caax protease) family

Names & Taxonomyi

Protein namesi
Recommended name:
CAAX prenyl protease 1 (EC:3.4.24.84)
Alternative name(s):
A-factor-converting enzyme
Prenyl protein-specific endoprotease 1
Short name:
PPSEP 1
Gene namesi
Name:STE24
Synonyms:AFC1
Ordered Locus Names:YJR117W
ORF Names:J2032
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR117W
SGDiS000003878 STE24

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12LumenalSequence analysisAdd BLAST12
Transmembranei13 – 33HelicalSequence analysisAdd BLAST21
Topological domaini34 – 89CytoplasmicSequence analysisAdd BLAST56
Transmembranei90 – 110HelicalSequence analysisAdd BLAST21
Topological domaini111 – 121LumenalSequence analysisAdd BLAST11
Transmembranei122 – 142HelicalSequence analysisAdd BLAST21
Topological domaini143 – 167CytoplasmicSequence analysisAdd BLAST25
Transmembranei168 – 188HelicalSequence analysisAdd BLAST21
Topological domaini189 – 197LumenalSequence analysis9
Transmembranei198 – 218HelicalSequence analysisAdd BLAST21
Topological domaini219 – 306CytoplasmicSequence analysisAdd BLAST88
Transmembranei307 – 327HelicalSequence analysisAdd BLAST21
Topological domaini328 – 357LumenalSequence analysisAdd BLAST30
Transmembranei358 – 378HelicalSequence analysisAdd BLAST21
Topological domaini379 – 453CytoplasmicSequence analysisAdd BLAST75

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1741185

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001388461 – 453CAAX prenyl protease 1Add BLAST453

Proteomic databases

MaxQBiP47154
PaxDbiP47154
PRIDEiP47154

Interactioni

Protein-protein interaction databases

BioGridi33873, 362 interactors
DIPiDIP-1390N
IntActiP47154, 25 interactors
MINTiP47154
STRINGi4932.YJR117W

Structurei

3D structure databases

ProteinModelPortaliP47154
SMRiP47154
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M48A family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000002053
HOGENOMiHOG000257874
InParanoidiP47154
KOiK06013
OMAiRFEYQAD
OrthoDBiEOG092C38HU

Family and domain databases

CDDicd07343 M48A_Zmpste24p_like, 1 hit
InterProiView protein in InterPro
IPR027057 CAXX_Prtase_1
IPR001915 Peptidase_M48
IPR032456 Peptidase_M48_N
PANTHERiPTHR10120 PTHR10120, 1 hit
PfamiView protein in Pfam
PF01435 Peptidase_M48, 1 hit
PF16491 Peptidase_M48_N, 1 hit
PROSITEiView protein in PROSITE
PS00142 ZINC_PROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

P47154-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFDLKTILDH PNIPWKLIIS GFSIAQFSFE SYLTYRQYQK LSETKLPPVL
60 70 80 90 100
EDEIDDETFH KSRNYSRAKA KFSIFGDVYN LAQKLVFIKY DLFPKIWHMA
110 120 130 140 150
VSLLNAVLPV RFHMVSTVAQ SLCFLGLLSS LSTLVDLPLS YYSHFVLEEK
160 170 180 190 200
FGFNKLTVQL WITDMIKSLT LAYAIGGPIL YLFLKIFDKF PTDFLWYIMV
210 220 230 240 250
FLFVVQILAM TIIPVFIMPM FNKFTPLEDG ELKKSIESLA DRVGFPLDKI
260 270 280 290 300
FVIDGSKRSS HSNAYFTGLP FTSKRIVLFD TLVNSNSTDE ITAVLAHEIG
310 320 330 340 350
HWQKNHIVNM VIFSQLHTFL IFSLFTSIYR NTSFYNTFGF FLEKSTGSFV
360 370 380 390 400
DPVITKEFPI IIGFMLFNDL LTPLECAMQF VMSLISRTHE YQADAYAKKL
410 420 430 440 450
GYKQNLCRAL IDLQIKNLST MNVDPLYSSY HYSHPTLAER LTALDYVSEK

KKN
Length:453
Mass (Da):52,324
Last modified:February 1, 1996 - v1
Checksum:i331CC9AE2D7C99DA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77137 Genomic DNA Translation: AAB38271.1
Z49617 Genomic DNA Translation: CAA89647.1
BK006943 Genomic DNA Translation: DAA08902.1
PIRiS57140
RefSeqiNP_012651.1, NM_001181775.1

Genome annotation databases

EnsemblFungiiYJR117W; YJR117W; YJR117W
GeneIDi853581
KEGGisce:YJR117W

Similar proteinsi

Entry informationi

Entry nameiSTE24_YEAST
AccessioniPrimary (citable) accession number: P47154
Secondary accession number(s): D6VWT6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: July 18, 2018
This is version 161 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

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