Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Diphthamide biosynthesis protein 4

Gene

JJJ3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for the first step of diphthamide biosynthesis, the transfer of 3-amino-3-carboxypropyl from S-adenosyl-L-methionine to a histidine residue. Diphthamide is a post-translational modification of histidine which occurs in elongation factor 2.1 Publication

Miscellaneous

Present with 2010 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri91 – 164DPH-typePROSITE-ProRule annotationAdd BLAST74

GO - Molecular functioni

  • ferrous iron binding Source: SGD

GO - Biological processi

  • peptidyl-diphthamide biosynthetic process from peptidyl-histidine Source: SGD

Keywordsi

LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:G3O-31725-MONOMER
YEAST:G3O-31725-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Diphthamide biosynthesis protein 4
Alternative name(s):
J protein type 3
Gene namesi
Name:JJJ3
Synonyms:DPH4
Ordered Locus Names:YJR097W
ORF Names:J1931
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

SGDiS000003858 JJJ3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000711542 – 172Diphthamide biosynthesis protein 4Add BLAST171

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP47138
PaxDbiP47138
PRIDEiP47138

PTM databases

iPTMnetiP47138

Interactioni

Protein-protein interaction databases

BioGridi33852, 131 interactors
DIPiDIP-4356N
IntActiP47138, 1 interactor
MINTiP47138
STRINGi4932.YJR097W

Structurei

3D structure databases

ProteinModelPortaliP47138
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 77JPROSITE-ProRule annotationAdd BLAST72

Sequence similaritiesi

Belongs to the DPH4 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri91 – 164DPH-typePROSITE-ProRule annotationAdd BLAST74

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOGENOMiHOG000189813
InParanoidiP47138
KOiK17867
OMAiCWYRGCR
OrthoDBiEOG092C4Z2R

Family and domain databases

CDDicd06257 DnaJ, 1 hit
Gene3Di1.10.287.110, 1 hit
3.10.660.10, 1 hit
InterProiView protein in InterPro
IPR001623 DnaJ_domain
IPR036869 J_dom_sf
IPR007872 Znf_DHP
IPR036671 Znf_DHP_sf
PfamiView protein in Pfam
PF00226 DnaJ, 1 hit
PF05207 zf-CSL, 1 hit
PRINTSiPR00625 JDOMAIN
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SUPFAMiSSF144217 SSF144217, 1 hit
SSF46565 SSF46565, 1 hit
PROSITEiView protein in PROSITE
PS50076 DNAJ_2, 1 hit
PS51074 ZF_DPH, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P47138-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSLVNSLTHY EILRIPSDAT QDEIKKAYRN RLLNTHPDKL SKSIHDTVSN
60 70 80 90 100
VTINKIQDAY KILSNIKTRR EYDRLILENY KRQGFHNCGD GLDEFSLDDF
110 120 130 140 150
SFDEDKLEFM MNCPRCQFVG GFHFSESLLD ECIDNVDAME RSHSGYQLLT
160 170
QCSACSLWLK VNFDIEEEQE GQ
Length:172
Mass (Da):20,027
Last modified:February 1, 1996 - v1
Checksum:i3BE6567ABC35EFF3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49597 Genomic DNA Translation: CAA89627.1
AY558542 Genomic DNA Translation: AAS56868.1
BK006943 Genomic DNA Translation: DAA08882.1
PIRiS57118
RefSeqiNP_012631.3, NM_001181755.3

Genome annotation databases

EnsemblFungiiYJR097W; YJR097W; YJR097W
GeneIDi853560
KEGGisce:YJR097W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49597 Genomic DNA Translation: CAA89627.1
AY558542 Genomic DNA Translation: AAS56868.1
BK006943 Genomic DNA Translation: DAA08882.1
PIRiS57118
RefSeqiNP_012631.3, NM_001181755.3

3D structure databases

ProteinModelPortaliP47138
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33852, 131 interactors
DIPiDIP-4356N
IntActiP47138, 1 interactor
MINTiP47138
STRINGi4932.YJR097W

PTM databases

iPTMnetiP47138

Proteomic databases

MaxQBiP47138
PaxDbiP47138
PRIDEiP47138

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR097W; YJR097W; YJR097W
GeneIDi853560
KEGGisce:YJR097W

Organism-specific databases

SGDiS000003858 JJJ3

Phylogenomic databases

HOGENOMiHOG000189813
InParanoidiP47138
KOiK17867
OMAiCWYRGCR
OrthoDBiEOG092C4Z2R

Enzyme and pathway databases

BioCyciMetaCyc:G3O-31725-MONOMER
YEAST:G3O-31725-MONOMER

Miscellaneous databases

PROiPR:P47138

Family and domain databases

CDDicd06257 DnaJ, 1 hit
Gene3Di1.10.287.110, 1 hit
3.10.660.10, 1 hit
InterProiView protein in InterPro
IPR001623 DnaJ_domain
IPR036869 J_dom_sf
IPR007872 Znf_DHP
IPR036671 Znf_DHP_sf
PfamiView protein in Pfam
PF00226 DnaJ, 1 hit
PF05207 zf-CSL, 1 hit
PRINTSiPR00625 JDOMAIN
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SUPFAMiSSF144217 SSF144217, 1 hit
SSF46565 SSF46565, 1 hit
PROSITEiView protein in PROSITE
PS50076 DNAJ_2, 1 hit
PS51074 ZF_DPH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDPH4_YEAST
AccessioniPrimary (citable) accession number: P47138
Secondary accession number(s): D6VWR6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 7, 2018
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again