UniProtKB - P47110 (DPOD3_YEAST)
Protein
DNA polymerase delta subunit 3
Gene
POL32
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
DNA polymerase delta (DNA polymerase III) participates in chromosomal DNA replication. It is required during synthesis of the leading and lagging DNA strands at the replication fork and binds at/or near replication origins and moves along DNA with the replication fork. It has 3'-5' proofreading exonuclease activity that correct errors arising during DNA replication. It is also involved in DNA synthesis during DNA repair.
Miscellaneous
Present with 2410 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- DNA-directed DNA polymerase activity Source: SGD
GO - Biological processi
- base-excision repair Source: SGD
- DNA amplification Source: SGD
- DNA replication, removal of RNA primer Source: SGD
- double-strand break repair via break-induced replication Source: SGD
- lagging strand elongation Source: SGD
- leading strand elongation Source: SGD
- nucleic acid phosphodiester bond hydrolysis Source: GOC
- nucleotide-excision repair Source: SGD
- postreplication repair Source: SGD
- RNA-dependent DNA biosynthetic process Source: SGD
Keywordsi
Molecular function | DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase |
Biological process | DNA replication |
Names & Taxonomyi
Protein namesi | Recommended name: DNA polymerase delta subunit 3 |
Gene namesi | Name:POL32 Ordered Locus Names:YJR043C ORF Names:J1626 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YJR043C |
SGDi | S000003804, POL32 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Nucleus
- delta DNA polymerase complex Source: SGD
- nucleus Source: SGD
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000186050 | 1 – 350 | DNA polymerase delta subunit 3Add BLAST | 350 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 223 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 230 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P47110 |
PaxDbi | P47110 |
PRIDEi | P47110 |
PTM databases
iPTMneti | P47110 |
Interactioni
Subunit structurei
DNA polymerase delta is a heterotrimer of POL3, POL32 and HYS2. POL32 can form homodimers.
Binary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 33794, 456 interactors |
ComplexPortali | CPX-2101, DNA polymerase delta complex |
DIPi | DIP-2523N |
ELMi | P47110 |
IntActi | P47110, 12 interactors |
MINTi | P47110 |
STRINGi | 4932.YJR043C |
Miscellaneous databases
RNActi | P47110, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P47110 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Phylogenomic databases
eggNOGi | ENOG502QW2D, Eukaryota |
HOGENOMi | CLU_794694_0_0_1 |
InParanoidi | P47110 |
OMAi | DCFIYAF |
i Sequence
Sequence statusi: Complete.
P47110-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDQKASYFIN EKLFTEVKPV LFTDLIHHLK IGPSMAKKLM FDYYKQTTNA
60 70 80 90 100
KYNCVVICCY KDQTIKIIHD LSNIPQQDSI IDCFIYAFNP MDSFIPYYDI
110 120 130 140 150
IDQKDCLTIK NSYELKVSES SKIIERTKTL EEKSKPLVRP TARSKTTPEE
160 170 180 190 200
TTGRKSKSKD MGLRSTALLA KMKKDRDDKE TSRQNELRKR KEENLQKINK
210 220 230 240 250
QNPEREAQMK ELNNLFVEDD LDTEEVNGGS KPNSPKETDS NDKDKNNDDL
260 270 280 290 300
EDLLETTAED SLMDVPKIQQ TKPSETEHSK EPKSEEEPSS FIDEDGYIVT
310 320 330 340 350
KRPATSTPPR KPSPVVKRAL SSSKKQETPS SNKRLKKQGT LESFFKRKAK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L36344 Genomic DNA Translation: AAA88745.1 Z49543 Genomic DNA Translation: CAA89571.1 AY557918 Genomic DNA Translation: AAS56244.1 BK006943 Genomic DNA Translation: DAA08830.1 |
PIRi | S57062 |
RefSeqi | NP_012577.1, NM_001181701.1 |
Genome annotation databases
EnsemblFungii | YJR043C_mRNA; YJR043C; YJR043C |
GeneIDi | 853500 |
KEGGi | sce:YJR043C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L36344 Genomic DNA Translation: AAA88745.1 Z49543 Genomic DNA Translation: CAA89571.1 AY557918 Genomic DNA Translation: AAS56244.1 BK006943 Genomic DNA Translation: DAA08830.1 |
PIRi | S57062 |
RefSeqi | NP_012577.1, NM_001181701.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6P1H | electron microscopy | 3.20 | C | 1-350 | [»] | |
6V8P | electron microscopy | 4.10 | G | 1-350 | [»] | |
6V93 | electron microscopy | 3.10 | G | 1-350 | [»] | |
SMRi | P47110 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 33794, 456 interactors |
ComplexPortali | CPX-2101, DNA polymerase delta complex |
DIPi | DIP-2523N |
ELMi | P47110 |
IntActi | P47110, 12 interactors |
MINTi | P47110 |
STRINGi | 4932.YJR043C |
PTM databases
iPTMneti | P47110 |
Proteomic databases
MaxQBi | P47110 |
PaxDbi | P47110 |
PRIDEi | P47110 |
Genome annotation databases
EnsemblFungii | YJR043C_mRNA; YJR043C; YJR043C |
GeneIDi | 853500 |
KEGGi | sce:YJR043C |
Organism-specific databases
EuPathDBi | FungiDB:YJR043C |
SGDi | S000003804, POL32 |
Phylogenomic databases
eggNOGi | ENOG502QW2D, Eukaryota |
HOGENOMi | CLU_794694_0_0_1 |
InParanoidi | P47110 |
OMAi | DCFIYAF |
Miscellaneous databases
PROi | PR:P47110 |
RNActi | P47110, protein |
Family and domain databases
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DPOD3_YEAST | |
Accessioni | P47110Primary (citable) accession number: P47110 Secondary accession number(s): D6VWL4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1996 |
Last sequence update: | February 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 165 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome X
Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names