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UniProtKB - P47058 (TAD2_YEAST)
Protein
tRNA-specific adenosine deaminase subunit TAD2
Gene
TAD2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Deaminates adenosine-34 to inosine in many tRNAs.
Miscellaneous
Present with 830 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:3.5.4.33
Cofactori
Zn2+By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 54 | Zinc; catalyticBy similarity | 1 | |
Active sitei | 56 | Proton donorBy similarity | 1 | |
Metal bindingi | 88 | Zinc; catalyticBy similarity | 1 | |
Metal bindingi | 91 | Zinc; catalyticBy similarity | 1 |
GO - Molecular functioni
- tRNA-specific adenosine-34 deaminase activity Source: GO_Central
- tRNA-specific adenosine deaminase activity Source: SGD
- zinc ion binding Source: InterPro
GO - Biological processi
- tRNA modification Source: SGD
- tRNA wobble adenosine to inosine editing Source: ComplexPortal
Keywordsi
Molecular function | Hydrolase |
Biological process | tRNA processing |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 3.5.4.33, 984 |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA-specific adenosine deaminase subunit TAD2 (EC:3.5.4.33)Alternative name(s): tRNA-specific adenosine-34 deaminase subunit TAD2 |
Gene namesi | Name:TAD2 Ordered Locus Names:YJL035C ORF Names:J1246 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003572, TAD2 |
VEuPathDBi | FungiDB:YJL035C |
Subcellular locationi
Nucleus
- nucleus Source: SGD
Other locations
- cytoplasm Source: SGD
- tRNA-specific adenosine-34 deaminase complex Source: ComplexPortal
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000171740 | 1 – 250 | tRNA-specific adenosine deaminase subunit TAD2Add BLAST | 250 |
Proteomic databases
PaxDbi | P47058 |
PRIDEi | P47058 |
Interactioni
Subunit structurei
Heterodimer with TAD3.
Binary interactionsi
P47058
With | #Exp. | IntAct |
---|---|---|
TAD3 [Q9URQ3] | 2 | EBI-18939,EBI-2094330 |
Protein-protein interaction databases
BioGRIDi | 33725, 90 interactors |
ComplexPortali | CPX-1742, tRNA-specific adenosine-34 deaminase complex |
DIPi | DIP-5546N |
IntActi | P47058, 1 interactor |
MINTi | P47058 |
STRINGi | 4932.YJL035C |
Miscellaneous databases
RNActi | P47058, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P47058 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 119 | CMP/dCMP-type deaminasePROSITE-ProRule annotationAdd BLAST | 119 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1018, Eukaryota |
GeneTreei | ENSGT00940000164335 |
HOGENOMi | CLU_025810_8_1_1 |
InParanoidi | P47058 |
OMAi | PCQMCAG |
Family and domain databases
InterProi | View protein in InterPro IPR016192, APOBEC/CMP_deaminase_Zn-bd IPR002125, CMP_dCMP_dom IPR016193, Cytidine_deaminase-like |
Pfami | View protein in Pfam PF00383, dCMP_cyt_deam_1, 1 hit |
SUPFAMi | SSF53927, SSF53927, 1 hit |
PROSITEi | View protein in PROSITE PS00903, CYT_DCMP_DEAMINASES_1, 1 hit PS51747, CYT_DCMP_DEAMINASES_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P47058-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQHIKHMRTA VRLARYALDH DETPVACIFV HTPTGQVMAY GMNDTNKSLT
60 70 80 90 100
GVAHAEFMGI DQIKAMLGSR GVVDVFKDIT LYVTVEPCIM CASALKQLDI
110 120 130 140 150
GKVVFGCGNE RFGGNGTVLS VNHDTCTLVP KNNSAAGYES IPGILRKEAI
160 170 180 190 200
MLLRYFYVRQ NERAPKPRSK SDRVLDKNTF PPMEWSKYLN EEAFIETFGD
210 220 230 240 250
DYRTCFANKV DLSSNSVDWD LIDSHQDNII QELEEQCKMF KFNVHKKSKV
Sequence cautioni
The sequence CAA88261 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ242667 Genomic DNA Translation: CAB60629.1 Z49310 Genomic DNA Translation: CAA89326.1 AY693172 Genomic DNA Translation: AAT93191.1 Z48229 Genomic DNA Translation: CAA88261.1 Different initiation. BK006943 Genomic DNA Translation: DAA08764.1 |
PIRi | S56807 |
RefSeqi | NP_012499.1, NM_001181469.1 |
Genome annotation databases
EnsemblFungii | YJL035C_mRNA; YJL035C; YJL035C |
GeneIDi | 853417 |
KEGGi | sce:YJL035C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ242667 Genomic DNA Translation: CAB60629.1 Z49310 Genomic DNA Translation: CAA89326.1 AY693172 Genomic DNA Translation: AAT93191.1 Z48229 Genomic DNA Translation: CAA88261.1 Different initiation. BK006943 Genomic DNA Translation: DAA08764.1 |
PIRi | S56807 |
RefSeqi | NP_012499.1, NM_001181469.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
7BV5 | X-ray | 2.80 | A/B | 2-250 | [»] | |
SMRi | P47058 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 33725, 90 interactors |
ComplexPortali | CPX-1742, tRNA-specific adenosine-34 deaminase complex |
DIPi | DIP-5546N |
IntActi | P47058, 1 interactor |
MINTi | P47058 |
STRINGi | 4932.YJL035C |
Proteomic databases
PaxDbi | P47058 |
PRIDEi | P47058 |
Genome annotation databases
EnsemblFungii | YJL035C_mRNA; YJL035C; YJL035C |
GeneIDi | 853417 |
KEGGi | sce:YJL035C |
Organism-specific databases
SGDi | S000003572, TAD2 |
VEuPathDBi | FungiDB:YJL035C |
Phylogenomic databases
eggNOGi | KOG1018, Eukaryota |
GeneTreei | ENSGT00940000164335 |
HOGENOMi | CLU_025810_8_1_1 |
InParanoidi | P47058 |
OMAi | PCQMCAG |
Enzyme and pathway databases
BRENDAi | 3.5.4.33, 984 |
Miscellaneous databases
PROi | PR:P47058 |
RNActi | P47058, protein |
Family and domain databases
InterProi | View protein in InterPro IPR016192, APOBEC/CMP_deaminase_Zn-bd IPR002125, CMP_dCMP_dom IPR016193, Cytidine_deaminase-like |
Pfami | View protein in Pfam PF00383, dCMP_cyt_deam_1, 1 hit |
SUPFAMi | SSF53927, SSF53927, 1 hit |
PROSITEi | View protein in PROSITE PS00903, CYT_DCMP_DEAMINASES_1, 1 hit PS51747, CYT_DCMP_DEAMINASES_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TAD2_YEAST | |
Accessioni | P47058Primary (citable) accession number: P47058 Secondary accession number(s): D6VWE8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1996 |
Last sequence update: | February 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 169 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome X
Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families