UniProtKB - P47033 (PRY3_YEAST)
Protein
Cell wall protein PRY3
Gene
PRY3
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
The full-length isoform (isoform Long) is a daughter cell-specific cell wall protein required for efficient export of lipids such as acetylated sterols. Acts in detoxification of hydrophobic compounds. Involved in tolerance to organic solvents such as dimethyl sulfoxide (DMSO). Plays also a role as an inhibitor of mating. STE12 is utilized as a repressor of full-length PRY3 transcription, ensuring efficient mating.
There is no evidence that production of the short PRY3 transcript (isoform Short) is anything more than an adventitious by-product of the mechanism responsible for the repression of the full-length transcript. Moreover, no disadvantage is detectable for cells unable to make the short transcript (PubMed:16940175).1 Publication
GO - Biological processi
- fatty acid transport Source: SGD
- sterol transport Source: SGD
Keywordsi
Biological process | Lipid transport, Transport |
Names & Taxonomyi
Protein namesi | Recommended name: Cell wall protein PRY3Alternative name(s): Pathogenesis-related protein 3 |
Gene namesi | Name:PRY3 Ordered Locus Names:YJL078C ORF Names:J1027 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003614, PRY3 |
VEuPathDBi | FungiDB:YJL078C |
Subcellular locationi
Cell wall
Other locations
Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP). The short isoform lacks the predicted signal peptide and might have an alternate localization.
Cell Wall
- fungal-type cell wall Source: SGD
Extracellular region or secreted
- extracellular space Source: GO_Central
Other locations
- anchored component of membrane Source: UniProtKB-KW
- cell periphery Source: SGD
Keywords - Cellular componenti
Cell wall, Membrane, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | Sequence analysisAdd BLAST | 18 | |
ChainiPRO_0000211549 | 19 – 853 | Cell wall protein PRY3Add BLAST | 835 | |
PropeptideiPRO_0000372447 | 854 – 881 | Removed in mature formSequence analysisAdd BLAST | 28 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 101 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 360 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 488 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 535 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 547 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 569 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 625 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Lipidationi | 853 | GPI-anchor amidated glycineSequence analysis | 1 |
Post-translational modificationi
The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.
Keywords - PTMi
Glycoprotein, GPI-anchor, LipoproteinProteomic databases
PaxDbi | P47033 |
PRIDEi | P47033 |
PTM databases
iPTMneti | P47033 |
Expressioni
Inductioni
Expressed specifically in daughter cells through activation by the transcription factor ACE2. STE12 represses expression of the full-length transcript and induces expression of the short form. STE12-binding to pheromone response elements (PREs) at positions -175 and -161 prevents SPT15 from binding TATA-box 1 and thus it binds TATA-box 2 at position +385 and directs internal transcription initiation at position +452.2 Publications
Interactioni
Protein-protein interaction databases
BioGRIDi | 33678, 120 interactors |
DIPi | DIP-3925N |
STRINGi | 4932.YJL078C |
Miscellaneous databases
RNActi | P47033, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 30 – 144 | SCPAdd BLAST | 115 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 163 – 845 | Ser/Thr-richAdd BLAST | 683 |
Sequence similaritiesi
Belongs to the CRISP family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3017, Eukaryota |
GeneTreei | ENSGT00980000198854 |
HOGENOMi | CLU_015197_0_0_1 |
InParanoidi | P47033 |
OMAi | NSVKFAN |
Family and domain databases
CDDi | cd05384, CAP_PRY1-like, 1 hit |
Gene3Di | 3.40.33.10, 1 hit |
InterProi | View protein in InterPro IPR018244, Allrgn_V5/Tpx1_CS IPR014044, CAP_domain IPR035940, CAP_sf IPR001283, CRISP-related IPR034120, SCP_Pry1-like |
PANTHERi | PTHR10334, PTHR10334, 1 hit |
Pfami | View protein in Pfam PF00188, CAP, 1 hit |
PRINTSi | PR00837, V5TPXLIKE |
SMARTi | View protein in SMART SM00198, SCP, 1 hit |
SUPFAMi | SSF55797, SSF55797, 1 hit |
PROSITEi | View protein in PROSITE PS01009, CRISP_1, 1 hit PS01010, CRISP_2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative promoter usage. AlignAdd to basketIsoform Long (identifier: P47033-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MLEFPISVLL GCLVAVKAQT TFPNFESDVL NEHNKFRALH VDTAPLTWSD
60 70 80 90 100
TLATYAQNYA DQYDCSGVLT HSDGPYGENL ALGYTDTGAV DAWYGEISKY
110 120 130 140 150
NYSNPGFSES TGHFTQVVWK STAEIGCGYK YCGTTWNNYI VCSYNPPGNY
160 170 180 190 200
LGEFAEEVEP LISTVSSSSS SSSSTSTTSD TVSTISSSIM PAVAQGYTTT
210 220 230 240 250
VSSAASSSSL KSTTINPAKT ATLTASSSTV ITSSTESVGS STVSSASSSS
260 270 280 290 300
VTTSYATSSS TVVSSDATSS TTTTSSVATS SSTTSSDPTS STAAASSSDP
310 320 330 340 350
ASSSAAASSS ASTENAASSS SAISSSSSMV SAPLSSTLTT STASSRSVTS
360 370 380 390 400
NSVNSVKFAN TTVFSAQTTS SVSASLSSSV AADDIQGSTS KEATSSVSEH
410 420 430 440 450
TSIVTSATNA AQYATRLGSS SRSSSGAVSS SAVSQSVLNS VIAVNTDVSV
460 470 480 490 500
TSVSSTAHTT KDTATTSVTA SESITSETAQ ASSSTEKNIS NSAATSSSIY
510 520 530 540 550
SNSASVSGHG VTYAAEYAIT SEQSSALATS VPATNCSSIV KTTTLENSST
560 570 580 590 600
TTITAITKST TTLATTANNS TRAATAVTID PTLDPTDNSA SPTDNAKHTS
610 620 630 640 650
TYGSSSTGAS LDSLRTTTSI SVSSNTTQLV STCTSESDYS DSPSFAISTA
660 670 680 690 700
TTTESNLITN TITASCSTDS NFPTSAASST DETAFTRTIS TSCSTLNGAS
710 720 730 740 750
TQTSELTTSP MKTNTVVPAS SFPSTTTTCL ENDDTAFSSI YTEVNAATII
760 770 780 790 800
NPGETSSLAS DFATSEKPNE PTSVKSTSNE GTSSTTTTYQ QTVATLYAKP
810 820 830 840 850
SSTSLGARTT TGSNGRSTTS QQDGSAMHQP TSSIYTQLKE GTSTTAKLSA
860 870 880
YEGAATPLSI FQCNSLAGTI AAFVVAVLFA F
Isoform Short (identifier: P47033-2)
Also known as: +452 PRY3
Sequence is not available
Note: Produced by alternative promoter usage.Curated
Length:–
Mass (Da):–
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X83502 Genomic DNA Translation: CAA58492.1 X88851 Genomic DNA Translation: CAA61314.1 Z49353 Genomic DNA Translation: CAA89370.1 BK006943 Genomic DNA Translation: DAA08721.1 |
PIRi | S56032 |
RefSeqi | NP_012457.1, NM_001181511.1 [P47033-1] |
Genome annotation databases
EnsemblFungii | YJL078C_mRNA; YJL078C; YJL078C [P47033-1] |
GeneIDi | 853367 |
KEGGi | sce:YJL078C |
Keywords - Coding sequence diversityi
Alternative promoter usageSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X83502 Genomic DNA Translation: CAA58492.1 X88851 Genomic DNA Translation: CAA61314.1 Z49353 Genomic DNA Translation: CAA89370.1 BK006943 Genomic DNA Translation: DAA08721.1 |
PIRi | S56032 |
RefSeqi | NP_012457.1, NM_001181511.1 [P47033-1] |
3D structure databases
SMRi | P47033 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 33678, 120 interactors |
DIPi | DIP-3925N |
STRINGi | 4932.YJL078C |
PTM databases
iPTMneti | P47033 |
Proteomic databases
PaxDbi | P47033 |
PRIDEi | P47033 |
Genome annotation databases
EnsemblFungii | YJL078C_mRNA; YJL078C; YJL078C [P47033-1] |
GeneIDi | 853367 |
KEGGi | sce:YJL078C |
Organism-specific databases
SGDi | S000003614, PRY3 |
VEuPathDBi | FungiDB:YJL078C |
Phylogenomic databases
eggNOGi | KOG3017, Eukaryota |
GeneTreei | ENSGT00980000198854 |
HOGENOMi | CLU_015197_0_0_1 |
InParanoidi | P47033 |
OMAi | NSVKFAN |
Miscellaneous databases
PROi | PR:P47033 |
RNActi | P47033, protein |
Family and domain databases
CDDi | cd05384, CAP_PRY1-like, 1 hit |
Gene3Di | 3.40.33.10, 1 hit |
InterProi | View protein in InterPro IPR018244, Allrgn_V5/Tpx1_CS IPR014044, CAP_domain IPR035940, CAP_sf IPR001283, CRISP-related IPR034120, SCP_Pry1-like |
PANTHERi | PTHR10334, PTHR10334, 1 hit |
Pfami | View protein in Pfam PF00188, CAP, 1 hit |
PRINTSi | PR00837, V5TPXLIKE |
SMARTi | View protein in SMART SM00198, SCP, 1 hit |
SUPFAMi | SSF55797, SSF55797, 1 hit |
PROSITEi | View protein in PROSITE PS01009, CRISP_1, 1 hit PS01010, CRISP_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PRY3_YEAST | |
Accessioni | P47033Primary (citable) accession number: P47033 Secondary accession number(s): D6VWA5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1996 |
Last sequence update: | February 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 144 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome X
Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names - SIMILARITY comments
Index of protein domains and families