UniProtKB - P47026 (GWT1_YEAST)
Protein
GPI-anchored wall transfer protein 1
Gene
GWT1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Probable acetyltransferase, which acetylates the inositol ring of phosphatidylinositol during biosynthesis of GPI-anchor. Acetylation during GPI-anchor biosynthesis is not essential for the subsequent mannosylation and is usually removed soon after the attachment of GPIs to proteins.2 Publications
Miscellaneous
Target of the antifungal compound 1-[4-butylbenzyl]isoquinoline that inhibits cell wall localization of GPI-anchored mannoproteins.
: glycosylphosphatidylinositol-anchor biosynthesis Pathwayi
This protein is involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis, which is part of Glycolipid biosynthesis.View all proteins of this organism that are known to be involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis and in Glycolipid biosynthesis.
GO - Molecular functioni
GO - Biological processi
- GPI anchor biosynthetic process Source: SGD
- protein localization to plasma membrane Source: GO_Central
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | GPI-anchor biosynthesis |
Enzyme and pathway databases
Reactomei | R-SCE-162710, Synthesis of glycosylphosphatidylinositol (GPI) |
UniPathwayi | UPA00196 |
Protein family/group databases
TCDBi | 9.B.315.1.5, the glycoslyphosphatidylinositol (gpi) membrane anchoring protein gwt1 (gwt1) family |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:GWT1 Ordered Locus Names:YJL091C ORF Names:J0916 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YJL091C |
SGDi | S000003627, GWT1 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 2 Publications; Multi-pass membrane protein 2 Publications
Endoplasmic reticulum
- endoplasmic reticulum Source: SGD
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: SGD
- nuclear outer membrane-endoplasmic reticulum membrane network Source: SGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 19 | LumenalSequence analysisAdd BLAST | 19 | |
Transmembranei | 20 – 40 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 41 – 54 | CytoplasmicSequence analysisAdd BLAST | 14 | |
Transmembranei | 55 – 75 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 76 | LumenalSequence analysis | 1 | |
Transmembranei | 77 – 97 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 98 – 128 | CytoplasmicSequence analysisAdd BLAST | 31 | |
Transmembranei | 129 – 149 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 150 – 157 | LumenalSequence analysis | 8 | |
Transmembranei | 158 – 178 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 179 – 199 | CytoplasmicSequence analysisAdd BLAST | 21 | |
Transmembranei | 200 – 220 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 221 – 231 | LumenalSequence analysisAdd BLAST | 11 | |
Transmembranei | 232 – 252 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 253 – 257 | CytoplasmicSequence analysis | 5 | |
Transmembranei | 258 – 278 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 279 – 301 | LumenalSequence analysisAdd BLAST | 23 | |
Transmembranei | 302 – 322 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 323 – 356 | CytoplasmicSequence analysisAdd BLAST | 34 | |
Transmembranei | 357 – 377 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 378 – 390 | LumenalSequence analysisAdd BLAST | 13 | |
Transmembranei | 391 – 411 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 412 – 435 | CytoplasmicSequence analysisAdd BLAST | 24 | |
Transmembranei | 436 – 456 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 457 – 460 | LumenalSequence analysis | 4 | |
Transmembranei | 461 – 481 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 482 – 490 | CytoplasmicSequence analysis | 9 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 8 | K → E in gwt1-10; impairs the transport of detergent-resistant microdomain-associated membrane proteins TAT2 and FUR4. 1 Publication | 1 | |
Mutagenesisi | 63 – 64 | WV → RA in gwt1-20; temperature sensitive mutant that induces a delay in export of GPI-anchored proteins. 1 Publication | 2 | |
Mutagenesisi | 132 | G → R: Resistant to the drug 1-[4-butylbenzyl]isoquinoline (BIQ). 1 Publication | 1 | |
Mutagenesisi | 209 | L → P in gwt1-28; temperature sensitive mutant that induces a delay in export of GPI-anchored proteins; when associated with D-259. 1 Publication | 1 | |
Mutagenesisi | 259 | V → D in gwt1-28; temperature sensitive mutant that induces a delay in export of GPI-anchored proteins; when associated with P-209. 1 Publication | 1 | |
Mutagenesisi | 330 | N → S in gwt1-16; temperature sensitive mutant that induces a delay in export of GPI-anchored proteins; when associated with P-362 and A-479. 1 Publication | 1 | |
Mutagenesisi | 362 | L → P in gwt1-16; temperature sensitive mutant that induces a delay in export of GPI-anchored proteins; when associated with S-330 and A-479. 1 Publication | 1 | |
Mutagenesisi | 397 | V → I: Resistant to the drug 1-[4-butylbenzyl]isoquinoline (BIQ). 1 Publication | 1 | |
Mutagenesisi | 479 | V → A in gwt1-16; temperature sensitive mutant that induces a delay in export of GPI-anchored proteins; when associated with S-330 and P-362. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000215188 | 1 – 490 | GPI-anchored wall transfer protein 1Add BLAST | 490 |
Proteomic databases
MaxQBi | P47026 |
PaxDbi | P47026 |
PRIDEi | P47026 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 33666, 457 interactors |
DIPi | DIP-4481N |
IntActi | P47026, 9 interactors |
MINTi | P47026 |
STRINGi | 4932.YJL091C |
Miscellaneous databases
RNActi | P47026, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the PIGW family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0411, Eukaryota |
GeneTreei | ENSGT00390000013520 |
HOGENOMi | CLU_020802_2_2_1 |
InParanoidi | P47026 |
OMAi | INLMVDT |
Family and domain databases
InterProi | View protein in InterPro IPR009447, PIGW/GWT1 |
PANTHERi | PTHR20661, PTHR20661, 1 hit |
Pfami | View protein in Pfam PF06423, GWT1, 1 hit |
PIRSFi | PIRSF017321, GWT1, 1 hit |
i Sequence
Sequence statusi: Complete.
P47026-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSTLKQRKED FVTGLNGGSI TEINAVTSIA LVTYISWNLL KNSNLMPPGI
60 70 80 90 100
SSVQYIIDFA LNWVALLLSI TIYASEPYLL NTLILLPCLL AFIYGKFTSS
110 120 130 140 150
SKPSNPIYNK KKMITQRFQL EKKPYITAYR GGMLILTAIA ILAVDFPIFP
160 170 180 190 200
RRFAKVETWG TSLMDLGVGS FVFSNGIVSS RALLKNLSLK SKPSFLKNAF
210 220 230 240 250
NALKSGGTLL FLGLLRLFFV KNLEYQEHVT EYGVHWNFFI TLSLLPLVLT
260 270 280 290 300
FIDPVTRMVP RCSIAIFISC IYEWLLLKDD RTLNFLILAD RNCFFSANRE
310 320 330 340 350
GIFSFLGYCS IFLWGQNTGF YLLGNKPTLN NLYKPSTQDV VAASKKSSTW
360 370 380 390 400
DYWTSVTPLS GLCIWSTIFL VISQLVFQYH PYSVSRRFAN LPYTLWVITY
410 420 430 440 450
NLLFLTGYCL TDKIFGNSSE YYKVAECLES INSNGLFLFL LANVSTGLVN
460 470 480 490
MSMVTIDSSP LKSFLVLLAY CSFIAVISVF LYRKRIFIKL
Sequence cautioni
The sequence CAA58476 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA89384 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z49366 Genomic DNA Translation: CAA89384.1 Different initiation. X83502 Genomic DNA Translation: CAA58476.1 Different initiation. BK006943 Genomic DNA Translation: DAA08709.1 |
PIRi | S56868 |
RefSeqi | NP_012444.2, NM_001181524.1 |
Genome annotation databases
EnsemblFungii | YJL091C_mRNA; YJL091C; YJL091C |
GeneIDi | 853354 |
KEGGi | sce:YJL091C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z49366 Genomic DNA Translation: CAA89384.1 Different initiation. X83502 Genomic DNA Translation: CAA58476.1 Different initiation. BK006943 Genomic DNA Translation: DAA08709.1 |
PIRi | S56868 |
RefSeqi | NP_012444.2, NM_001181524.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 33666, 457 interactors |
DIPi | DIP-4481N |
IntActi | P47026, 9 interactors |
MINTi | P47026 |
STRINGi | 4932.YJL091C |
Protein family/group databases
TCDBi | 9.B.315.1.5, the glycoslyphosphatidylinositol (gpi) membrane anchoring protein gwt1 (gwt1) family |
Proteomic databases
MaxQBi | P47026 |
PaxDbi | P47026 |
PRIDEi | P47026 |
Genome annotation databases
EnsemblFungii | YJL091C_mRNA; YJL091C; YJL091C |
GeneIDi | 853354 |
KEGGi | sce:YJL091C |
Organism-specific databases
EuPathDBi | FungiDB:YJL091C |
SGDi | S000003627, GWT1 |
Phylogenomic databases
eggNOGi | KOG0411, Eukaryota |
GeneTreei | ENSGT00390000013520 |
HOGENOMi | CLU_020802_2_2_1 |
InParanoidi | P47026 |
OMAi | INLMVDT |
Enzyme and pathway databases
UniPathwayi | UPA00196 |
Reactomei | R-SCE-162710, Synthesis of glycosylphosphatidylinositol (GPI) |
Miscellaneous databases
PROi | PR:P47026 |
RNActi | P47026, protein |
Family and domain databases
InterProi | View protein in InterPro IPR009447, PIGW/GWT1 |
PANTHERi | PTHR20661, PTHR20661, 1 hit |
Pfami | View protein in Pfam PF06423, GWT1, 1 hit |
PIRSFi | PIRSF017321, GWT1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GWT1_YEAST | |
Accessioni | P47026Primary (citable) accession number: P47026 Secondary accession number(s): D6VW93 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1996 |
Last sequence update: | October 31, 2006 | |
Last modified: | December 2, 2020 | |
This is version 147 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome X
Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names